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apy0201  (MedChemExpress)
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MedChemExpress apy0201
<t>APY0201</t> inhibits proliferation of GC cells. (a) Molecular structure of APY0201. (b) AGS, SGC7901, BGC823, MKN28, and SNU719 cells were treated with varied concentrations of APY0201 for 48 h and cell viability was determined. (c) Colony formation in AGS and SGC7901 cells treated with various concentrations of APY0201 for 14 days. (d)-(e) Statistical analysis of the numbers of colonies in part c. (f), (h) The EdU proliferation test was used to investigate the effects of varied concentrations of APY0201 on AGS and SGC7901 cells for 48 h. The scale bar is 300 μ m. (g), (i) Proportions of EdU-positive cells in AGS and SGC7901 cells. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 compared with 0 μ M APY0201 group.
Apy0201, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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APY0201 inhibits proliferation of GC cells. (a) Molecular structure of APY0201. (b) AGS, SGC7901, BGC823, MKN28, and SNU719 cells were treated with varied concentrations of APY0201 for 48 h and cell viability was determined. (c) Colony formation in AGS and SGC7901 cells treated with various concentrations of APY0201 for 14 days. (d)-(e) Statistical analysis of the numbers of colonies in part c. (f), (h) The EdU proliferation test was used to investigate the effects of varied concentrations of APY0201 on AGS and SGC7901 cells for 48 h. The scale bar is 300 μ m. (g), (i) Proportions of EdU-positive cells in AGS and SGC7901 cells. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 compared with 0 μ M APY0201 group.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201 inhibits proliferation of GC cells. (a) Molecular structure of APY0201. (b) AGS, SGC7901, BGC823, MKN28, and SNU719 cells were treated with varied concentrations of APY0201 for 48 h and cell viability was determined. (c) Colony formation in AGS and SGC7901 cells treated with various concentrations of APY0201 for 14 days. (d)-(e) Statistical analysis of the numbers of colonies in part c. (f), (h) The EdU proliferation test was used to investigate the effects of varied concentrations of APY0201 on AGS and SGC7901 cells for 48 h. The scale bar is 300 μ m. (g), (i) Proportions of EdU-positive cells in AGS and SGC7901 cells. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 compared with 0 μ M APY0201 group.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques:

APY0201 inhibits the growth of GC organoids. GC organoids were treated with various concentrations of APY0201 for 3 days. (a) Light microscope photos of GC organoids. The scale bar is 300 μ m. (b) GC organoids were tested for cell viability by the CCK8 assay, and a broken line graph was created to illustrate this.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201 inhibits the growth of GC organoids. GC organoids were treated with various concentrations of APY0201 for 3 days. (a) Light microscope photos of GC organoids. The scale bar is 300 μ m. (b) GC organoids were tested for cell viability by the CCK8 assay, and a broken line graph was created to illustrate this.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques: Light Microscopy, CCK-8 Assay

APY0201 inhibits the growth of GC xenografts. (a) Photographs of subcutaneous transplanted tumors and nude mice. (b) The average weight of transplanted tumors. (c) The volume of the transplanted tumor in each nude mouse was measured every 2 days, and a tumor growth curve during APY0201 treatment was plotted. (d) Mice were weighed every 2 days and a body weight change curve during APY0201 treatment was plotted.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201 inhibits the growth of GC xenografts. (a) Photographs of subcutaneous transplanted tumors and nude mice. (b) The average weight of transplanted tumors. (c) The volume of the transplanted tumor in each nude mouse was measured every 2 days, and a tumor growth curve during APY0201 treatment was plotted. (d) Mice were weighed every 2 days and a body weight change curve during APY0201 treatment was plotted.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques:

APY0201 induces autophagosome accumulation. (a)-(b) AGS and SGC7901 cells were treated with APY0201 at various concentrations for 24 h and the expressions of LC3 and p62 were determined by western blotting. (c)-(d) AGS and SGC7901 cells were treated with DMSO and APY0201 with or without BafA1 for 24 h and the expressions of LC3 and p62 were determined by western blotting. (e), (g) AGS and SGC7901 cells stably expressing StubRFP-SensGFP-LC3 were seeded onto confocal culture dishes and treated with different drugs for 24 h. Confocal microscopy revealed the development of LC3 dots in AGS and SGC7901 cells ( λ ex/green = 488 nm, λ em/green = 500–530 nm; λ ex/red = 545 nm, λ em/red = 590–630 nm). The scale bar is 25 μ m. (f), (h) Histograms were based on the numbers of yellow and red dots in different drug groups shown in parts (e) and (g). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 were compared with autophagosomes in the control group; # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 were compared with autolysosomes in the control group.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201 induces autophagosome accumulation. (a)-(b) AGS and SGC7901 cells were treated with APY0201 at various concentrations for 24 h and the expressions of LC3 and p62 were determined by western blotting. (c)-(d) AGS and SGC7901 cells were treated with DMSO and APY0201 with or without BafA1 for 24 h and the expressions of LC3 and p62 were determined by western blotting. (e), (g) AGS and SGC7901 cells stably expressing StubRFP-SensGFP-LC3 were seeded onto confocal culture dishes and treated with different drugs for 24 h. Confocal microscopy revealed the development of LC3 dots in AGS and SGC7901 cells ( λ ex/green = 488 nm, λ em/green = 500–530 nm; λ ex/red = 545 nm, λ em/red = 590–630 nm). The scale bar is 25 μ m. (f), (h) Histograms were based on the numbers of yellow and red dots in different drug groups shown in parts (e) and (g). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 were compared with autophagosomes in the control group; # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 were compared with autolysosomes in the control group.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques: Western Blot, Stable Transfection, Expressing, Confocal Microscopy, Control

APY0201 impairs the degradation function of lysosomes by inhibiting the activity of cathepsin in GC cells. (a)-(b) AGS and SGC7901 cells were treated with APY0201 at various concentrations for 24 h and the expressions of CTSD and CTSB were detected by western blotting.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201 impairs the degradation function of lysosomes by inhibiting the activity of cathepsin in GC cells. (a)-(b) AGS and SGC7901 cells were treated with APY0201 at various concentrations for 24 h and the expressions of CTSD and CTSB were detected by western blotting.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques: Activity Assay, Western Blot

APY0201-induced autophagosome accumulation inhibits proliferation of GC cells. AGS and SGC7901 cells were infected with lentiviruses carrying shNC and shATG5 genes. Subsequent experiments were carried out with AGS and SGC7901 cells stably expressing shNC and shATG5. (a, b) After 24 h of treatment with DMSO and APY0201, AGS and SGC7901 cells were examined by western blotting for ATG5 and LC3 expression. (c), (d) DMSO and APY0201 were applied to AGS and SGC7901 cells for 48 h and their viability was determined by CCK8 assay. Histograms were then drawn. ∗∗∗∗ P < 0.0001; ns, no statistically significant difference compared with the shNC group.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201-induced autophagosome accumulation inhibits proliferation of GC cells. AGS and SGC7901 cells were infected with lentiviruses carrying shNC and shATG5 genes. Subsequent experiments were carried out with AGS and SGC7901 cells stably expressing shNC and shATG5. (a, b) After 24 h of treatment with DMSO and APY0201, AGS and SGC7901 cells were examined by western blotting for ATG5 and LC3 expression. (c), (d) DMSO and APY0201 were applied to AGS and SGC7901 cells for 48 h and their viability was determined by CCK8 assay. Histograms were then drawn. ∗∗∗∗ P < 0.0001; ns, no statistically significant difference compared with the shNC group.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques: Infection, Stable Transfection, Expressing, Western Blot, CCK-8 Assay

APY0201 induces G1/S phase arrest of GC cells. (a), (c) After 24 h of DMSO and APY0201 treatment, the cell cycle of AGS and SGC7901 cells was examined by flow cytometry. (b), (d) Percentage distribution of cell cycle phases in the control group and APY0201 group. ∗ P < 0.05, ∗∗ P < 0.01; ns, no statistically significant difference compared with control group. (e), (f) AGS and SGC7901 cells were treated with APY0201 at various concentrations for 24 h. The expression levels of CDK2, CDK4, CDK6, cyclin D1, cyclin E1, p21, and p27 were analyzed by western blotting.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201 induces G1/S phase arrest of GC cells. (a), (c) After 24 h of DMSO and APY0201 treatment, the cell cycle of AGS and SGC7901 cells was examined by flow cytometry. (b), (d) Percentage distribution of cell cycle phases in the control group and APY0201 group. ∗ P < 0.05, ∗∗ P < 0.01; ns, no statistically significant difference compared with control group. (e), (f) AGS and SGC7901 cells were treated with APY0201 at various concentrations for 24 h. The expression levels of CDK2, CDK4, CDK6, cyclin D1, cyclin E1, p21, and p27 were analyzed by western blotting.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques: Flow Cytometry, Control, Expressing, Western Blot

APY0201 inhibits the proliferation of GC transplanted tumor cells by interrupting autophagic flux and cell cycle arrest. (a) The expression of LC3, p62, and p21 in transplanted tumors was analyzed by western blotting. (b) The expression of Ki67, LC3, p62, and p21 in transplanted tumors was detected by immunohistochemistry. The scale bar is 200 μ m.

Journal: Journal of Oncology

Article Title: APY0201 Represses Tumor Growth through Inhibiting Autophagy in Gastric Cancer Cells

doi: 10.1155/2022/7104592

Figure Lengend Snippet: APY0201 inhibits the proliferation of GC transplanted tumor cells by interrupting autophagic flux and cell cycle arrest. (a) The expression of LC3, p62, and p21 in transplanted tumors was analyzed by western blotting. (b) The expression of Ki67, LC3, p62, and p21 in transplanted tumors was detected by immunohistochemistry. The scale bar is 200 μ m.

Article Snippet: APY0201, bafilomycin A1 (BafA1), and rapamycin (Rapa) were obtained from MedChemExpress (MCE, Shanghai, China) and were dissolved in DMSO (Sigma, USA) for in vitro experiments.

Techniques: Expressing, Western Blot, Immunohistochemistry