Journal: Experimental and Therapeutic Medicine
Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis
Figure Lengend Snippet: Western blot analysis of dysregulated genes following treatment with podofilox. Immunoblots of HK2, PKM2, p53, c-Myc, ATG10 and GAPDH following incubation with vehicle or 3.4 nM podofilox for 48 h are presented on the left, and the quantitative analysis is presented on the right. * P<0.05 and ** P<0.01 vs. vehicle. HK2, hexokinase 2; PKM2, pyruvate kinase M1/2; ATG10, autophagy-related 10.
Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).
Techniques: Western Blot, Incubation