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  • vhl  (ATCC)
    99
    ATCC vhl
    Schematic representation of the role of beta-catenin and MEK1 <t>in</t> <t>VHL-inactivated</t> renal cancer cells and potential miRNAs targeting beta-catenin and MEK1 based on miRDB. (A) When the VHL gene is mutated or inactivated, protein VHL (p-VHL) fails to regulate HIF-1 and beta-catenin, resulting in constitutive HIF activation and aberrant accumulation of cytoplasmic and nuclear beta-catenin. (B). Based on miRDB, several miRNAs targeting beta-catenin and HIF downstream genes (MEK1/2 and ERK1/2) were identified.
    Vhl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vhl/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vhl - by Bioz Stars, 2024-05
    99/100 stars
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    93
    ATCC transfection human kidney carcinoma epithelial cells a498
    Schematic representation of the role of beta-catenin and MEK1 <t>in</t> <t>VHL-inactivated</t> renal cancer cells and potential miRNAs targeting beta-catenin and MEK1 based on miRDB. (A) When the VHL gene is mutated or inactivated, protein VHL (p-VHL) fails to regulate HIF-1 and beta-catenin, resulting in constitutive HIF activation and aberrant accumulation of cytoplasmic and nuclear beta-catenin. (B). Based on miRDB, several miRNAs targeting beta-catenin and HIF downstream genes (MEK1/2 and ERK1/2) were identified.
    Transfection Human Kidney Carcinoma Epithelial Cells A498, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection human kidney carcinoma epithelial cells a498/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transfection human kidney carcinoma epithelial cells a498 - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Schematic representation of the role of beta-catenin and MEK1 in VHL-inactivated renal cancer cells and potential miRNAs targeting beta-catenin and MEK1 based on miRDB. (A) When the VHL gene is mutated or inactivated, protein VHL (p-VHL) fails to regulate HIF-1 and beta-catenin, resulting in constitutive HIF activation and aberrant accumulation of cytoplasmic and nuclear beta-catenin. (B). Based on miRDB, several miRNAs targeting beta-catenin and HIF downstream genes (MEK1/2 and ERK1/2) were identified.

    Journal: Carcinogenesis

    Article Title: MicroRNA-1826 directly targets beta-catenin (CTNNB1) and MEK1 (MAP2K1) in VHL-inactivated renal cancer

    doi: 10.1093/carcin/bgr302

    Figure Lengend Snippet: Schematic representation of the role of beta-catenin and MEK1 in VHL-inactivated renal cancer cells and potential miRNAs targeting beta-catenin and MEK1 based on miRDB. (A) When the VHL gene is mutated or inactivated, protein VHL (p-VHL) fails to regulate HIF-1 and beta-catenin, resulting in constitutive HIF activation and aberrant accumulation of cytoplasmic and nuclear beta-catenin. (B). Based on miRDB, several miRNAs targeting beta-catenin and HIF downstream genes (MEK1/2 and ERK1/2) were identified.

    Article Snippet: Cell culture Renal cancer cell lines with inactivated VHL [A-498, American Type Culture Collection (ATCC) number: HTB-44; 786-O, ATCC number: CRL-1932] and with intact VHL (Caki-1, ATCC number: HTB-46) were purchased from the ATCC (Manassas, VA).

    Techniques: Activation Assay

    Binding of miR-1826 to the 3′ UTR of CTNNB1 and MEK1 mRNAs, luciferase assays and an inverse correlation between miR-1826 and CTNNB1/MEK1 protein expression in human renal cancer tissues. (A) CTNNB1 and MEK1 3′ UTR position and complementary miR-1826-binding sequence. (B) 3′ UTR luciferase assay (miR-NC and miR-1826), MT stands for mutated plasmid sequence and WT stands for wild-type plasmid sequence. (C) CTNNB1, survivin, MEK1, beta-tubulin protein expression in miR-NC or miR-1826-transfected VHL-inactivated renal cancer cells (A-498 and 786-O). (D) Inverse correlation between miR-1826 and CTNNB1/MEK1 protein expression in renal cancer tissues.

    Journal: Carcinogenesis

    Article Title: MicroRNA-1826 directly targets beta-catenin (CTNNB1) and MEK1 (MAP2K1) in VHL-inactivated renal cancer

    doi: 10.1093/carcin/bgr302

    Figure Lengend Snippet: Binding of miR-1826 to the 3′ UTR of CTNNB1 and MEK1 mRNAs, luciferase assays and an inverse correlation between miR-1826 and CTNNB1/MEK1 protein expression in human renal cancer tissues. (A) CTNNB1 and MEK1 3′ UTR position and complementary miR-1826-binding sequence. (B) 3′ UTR luciferase assay (miR-NC and miR-1826), MT stands for mutated plasmid sequence and WT stands for wild-type plasmid sequence. (C) CTNNB1, survivin, MEK1, beta-tubulin protein expression in miR-NC or miR-1826-transfected VHL-inactivated renal cancer cells (A-498 and 786-O). (D) Inverse correlation between miR-1826 and CTNNB1/MEK1 protein expression in renal cancer tissues.

    Article Snippet: Cell culture Renal cancer cell lines with inactivated VHL [A-498, American Type Culture Collection (ATCC) number: HTB-44; 786-O, ATCC number: CRL-1932] and with intact VHL (Caki-1, ATCC number: HTB-46) were purchased from the ATCC (Manassas, VA).

    Techniques: Binding Assay, Luciferase, Expressing, Sequencing, Plasmid Preparation, Transfection

    Effect of microR-1826 overexpression on VHL-inactivated renal cancer cells (A-498 and 786-O). (A) Cell viability assay (miR-NC or miR-1826-transfected A-498 cells and 786-O cells), (B) Invasion assay, (C) Wound healing assay, (D) Flow cytometry analysis of apoptosis in miR-NC or miR-1826-transfected A-498 cells. Data are the mean ± SD of four independent experiments.

    Journal: Carcinogenesis

    Article Title: MicroRNA-1826 directly targets beta-catenin (CTNNB1) and MEK1 (MAP2K1) in VHL-inactivated renal cancer

    doi: 10.1093/carcin/bgr302

    Figure Lengend Snippet: Effect of microR-1826 overexpression on VHL-inactivated renal cancer cells (A-498 and 786-O). (A) Cell viability assay (miR-NC or miR-1826-transfected A-498 cells and 786-O cells), (B) Invasion assay, (C) Wound healing assay, (D) Flow cytometry analysis of apoptosis in miR-NC or miR-1826-transfected A-498 cells. Data are the mean ± SD of four independent experiments.

    Article Snippet: Cell culture Renal cancer cell lines with inactivated VHL [A-498, American Type Culture Collection (ATCC) number: HTB-44; 786-O, ATCC number: CRL-1932] and with intact VHL (Caki-1, ATCC number: HTB-46) were purchased from the ATCC (Manassas, VA).

    Techniques: Over Expression, Viability Assay, Transfection, Invasion Assay, Wound Healing Assay, Flow Cytometry