Article Title: Expression of the onconeural protein CDR1 in cerebellum and ovarian cancer
Figure Lengend Snippet: CDR1 antibodies recognize cells overexpressing myc/DDK-CDR1 (A) Chicken CDR1 antibody (red) binds specifically to cells overexpressing myc-/DDK-CDR1. Anti-DDK (green) was used to identify transfected cells. The nucleus was visualized with DAPI (blue). Scale bar 20 μm. (B) Western blot with CDR1 rabbit antibody. Lane 1: Untransfected HeLa cell lysate, 4 μg; Lane 2: Lysate of HeLa cells overexpressing CDR1, 4 μg; Lane 3: Human cerebellum, 15 μg; Lane 4: Normal ovary, 20 μg; Lane 5: Ovarian tumor, 20 μg; Lane 6: Normal breast, 20 μg; Lane 7: Breast tumor, 20 μg; Lane 8: BT474 lysate, 20 μg; Lane 9: OVCAR 3 lysate, 15 μg; Lane 10: Human Purkinje cell lysate, 5 μg.
Article Snippet: BT474 cells (ATCC, #HTB-20) were grown in RPMI-1640 supplemented with 0.01 mg/ml insulin, 10% hi FBS, and 1% penicillin/streptomycin.
Techniques: Transfection, Western Blot