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  • bt474  (ATCC)
    98
    ATCC bt474
    CDR1 expression in cancer cell lines (A) Overexpression of myc/DDK-CDR1 in transfected HeLa cells: Left image: Hela cells transfected with myc/DDK-CDR1 (18 h transfection) and stained with anti-myc (green). CDR1 accumulates in the front-line of the cell. Right image: Cells transfected with CDR1-GFP (24 h transfection). CDR1 accumulates in the protrusions of the cell. Scale bar 20 μm. (B) Both OVCAR3 and <t>BT474</t> express CDR1 endogenously. The staining is stronger in the cells making the outer border of the cell colony. (C) Endogenous CDR1 expression (green) in OVCAR3 cells co-stained with the actin marker phalloidin (red). CDR1 is strongly expressed close to the plasma membrane and in the cytoplasm. CDR1 is not present in an area between the cortical actin and the outer membrane ruffles. However, CDR1 is expressed in the membrane protrusions where actin is involved in lamellipodia/filopodia formation. Main images are flattened images of the stack. Boxed areas are zoom in on a single section if the confocal plane. CDR1 co-localizes with actin in the membrane ruffles. Scale bar 10 μm. Nuclei are stained with DAPI (blue).
    Bt474, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CDR1 expression in cancer cell lines (A) Overexpression of myc/DDK-CDR1 in transfected HeLa cells: Left image: Hela cells transfected with myc/DDK-CDR1 (18 h transfection) and stained with anti-myc (green). CDR1 accumulates in the front-line of the cell. Right image: Cells transfected with CDR1-GFP (24 h transfection). CDR1 accumulates in the protrusions of the cell. Scale bar 20 μm. (B) Both OVCAR3 and BT474 express CDR1 endogenously. The staining is stronger in the cells making the outer border of the cell colony. (C) Endogenous CDR1 expression (green) in OVCAR3 cells co-stained with the actin marker phalloidin (red). CDR1 is strongly expressed close to the plasma membrane and in the cytoplasm. CDR1 is not present in an area between the cortical actin and the outer membrane ruffles. However, CDR1 is expressed in the membrane protrusions where actin is involved in lamellipodia/filopodia formation. Main images are flattened images of the stack. Boxed areas are zoom in on a single section if the confocal plane. CDR1 co-localizes with actin in the membrane ruffles. Scale bar 10 μm. Nuclei are stained with DAPI (blue).

    Journal: Oncotarget

    Article Title: Expression of the onconeural protein CDR1 in cerebellum and ovarian cancer

    doi: 10.18632/oncotarget.25252

    Figure Lengend Snippet: CDR1 expression in cancer cell lines (A) Overexpression of myc/DDK-CDR1 in transfected HeLa cells: Left image: Hela cells transfected with myc/DDK-CDR1 (18 h transfection) and stained with anti-myc (green). CDR1 accumulates in the front-line of the cell. Right image: Cells transfected with CDR1-GFP (24 h transfection). CDR1 accumulates in the protrusions of the cell. Scale bar 20 μm. (B) Both OVCAR3 and BT474 express CDR1 endogenously. The staining is stronger in the cells making the outer border of the cell colony. (C) Endogenous CDR1 expression (green) in OVCAR3 cells co-stained with the actin marker phalloidin (red). CDR1 is strongly expressed close to the plasma membrane and in the cytoplasm. CDR1 is not present in an area between the cortical actin and the outer membrane ruffles. However, CDR1 is expressed in the membrane protrusions where actin is involved in lamellipodia/filopodia formation. Main images are flattened images of the stack. Boxed areas are zoom in on a single section if the confocal plane. CDR1 co-localizes with actin in the membrane ruffles. Scale bar 10 μm. Nuclei are stained with DAPI (blue).

    Article Snippet: BT474 cells (ATCC, #HTB-20) were grown in RPMI-1640 supplemented with 0.01 mg/ml insulin, 10% hi FBS, and 1% penicillin/streptomycin.

    Techniques: Expressing, Over Expression, Transfection, Staining, Marker

    CDR1 antibodies recognize cells overexpressing myc/DDK-CDR1 (A) Chicken CDR1 antibody (red) binds specifically to cells overexpressing myc-/DDK-CDR1. Anti-DDK (green) was used to identify transfected cells. The nucleus was visualized with DAPI (blue). Scale bar 20 μm. (B) Western blot with CDR1 rabbit antibody. Lane 1: Untransfected HeLa cell lysate, 4 μg; Lane 2: Lysate of HeLa cells overexpressing CDR1, 4 μg; Lane 3: Human cerebellum, 15 μg; Lane 4: Normal ovary, 20 μg; Lane 5: Ovarian tumor, 20 μg; Lane 6: Normal breast, 20 μg; Lane 7: Breast tumor, 20 μg; Lane 8: BT474 lysate, 20 μg; Lane 9: OVCAR 3 lysate, 15 μg; Lane 10: Human Purkinje cell lysate, 5 μg.

    Journal: Oncotarget

    Article Title: Expression of the onconeural protein CDR1 in cerebellum and ovarian cancer

    doi: 10.18632/oncotarget.25252

    Figure Lengend Snippet: CDR1 antibodies recognize cells overexpressing myc/DDK-CDR1 (A) Chicken CDR1 antibody (red) binds specifically to cells overexpressing myc-/DDK-CDR1. Anti-DDK (green) was used to identify transfected cells. The nucleus was visualized with DAPI (blue). Scale bar 20 μm. (B) Western blot with CDR1 rabbit antibody. Lane 1: Untransfected HeLa cell lysate, 4 μg; Lane 2: Lysate of HeLa cells overexpressing CDR1, 4 μg; Lane 3: Human cerebellum, 15 μg; Lane 4: Normal ovary, 20 μg; Lane 5: Ovarian tumor, 20 μg; Lane 6: Normal breast, 20 μg; Lane 7: Breast tumor, 20 μg; Lane 8: BT474 lysate, 20 μg; Lane 9: OVCAR 3 lysate, 15 μg; Lane 10: Human Purkinje cell lysate, 5 μg.

    Article Snippet: BT474 cells (ATCC, #HTB-20) were grown in RPMI-1640 supplemented with 0.01 mg/ml insulin, 10% hi FBS, and 1% penicillin/streptomycin.

    Techniques: Transfection, Western Blot