Journal: Oncotarget

Article Title: The PI3K inhibitor GDC-0941 displays promising in vitro and in vivo efficacy for targeted medulloblastoma therapy

doi:

Figure Lengend Snippet: GDC-0941 decreased the cell viability of the established medulloblastoma cell lines MEB-Med-8A, D283 Med, Daoy and D341 Med. The cell lines were treated with increasing concentrations of GDC-0941. Area shaded in gray indicates the range of GDC-0941 concentration detected in patient's levels. The vehicle DMSO served as control. After 48 h of drug exposure the cell viability was assessed by means of the MTS assay. Data points below asterisks differ significantly (* p < 0.05) from the control. Each experiment was performed in triplicates and repeated four times.

Article Snippet: The human medulloblastoma cell lines Daoy (HTB 186), D283 Med (HTB185) and D341 Med (HTB-187) were obtained from American Type Culture Collection.

Techniques: Concentration Assay, MTS Assay

Journal: Oncotarget

Article Title: The PI3K inhibitor GDC-0941 displays promising in vitro and in vivo efficacy for targeted medulloblastoma therapy

doi:

Figure Lengend Snippet: In a combined proliferation-apoptosis assay based on a CFSE-7AAD-Annexin-V staining the capacity of GDC-0941 to inhibit proliferation (A) and induce apoptosis (B) in the stated medulloblastoma cell lines was determined. The cells were treated with 1 μM and 10 μM of GDC-0941 for 24 and 48 h respectively. The vehicle DMSO served as control. In contrast to apoptosis, proliferation inhibition was normalized to the control DMSO. All stated average values for proliferation inhibition and apoptosis induction differed significantly ( p < 0.05) from the DMSO control except the value for induction of apoptosis at 1 μM for the cell line D341 Med. The data represents four independent experiments. (C) upper panel - GDC-0941 induces a G1-phase cell cycle arrest. Daoy and MEB-Med8A cells were exposed to 1 and 10 μM of GDC-0941 for 48 h. Subsequently the cell cycle distribution was determined by Hoechst 33342 staining. The vehicle DMSO served as control. The lower panel in Figure visualizes the effect of GDC-0941 on the adherent cell lines Daoy and MEB-Med-8A. Statistically significant differences are marked by an asterisk (* p < 0.05). The data shown represents four independent experiments. The reduction in cell density and change in cell morphology is depicted. Scale bar 100 μm.

Article Snippet: The human medulloblastoma cell lines Daoy (HTB 186), D283 Med (HTB185) and D341 Med (HTB-187) were obtained from American Type Culture Collection.

Techniques: Apoptosis Assay, Staining, Inhibition

Journal: Oncotarget

Article Title: The PI3K inhibitor GDC-0941 displays promising in vitro and in vivo efficacy for targeted medulloblastoma therapy

doi:

Figure Lengend Snippet: In a orthotopic xenograft mouse model we analyzed whether GDC-0941 could inhibit medulloblastoma growth in vivo . For this purpose 2 × 10 4 MEB-Med-8A cells were transplanted into the cerebellum. The mice analyzed for tumor growth by bioluminescent imagining at 1, 2, 3 and 4 weeks. One week after transplantation mice were treated with 100mg/kg of GDC-0941 once daily until symptoms occurred. (A) depicts the normalized tumor growth delay while (B) shows the survival of treated and untreated animals via Kaplan-Meier plot. GDC-0941 treatment prolonged the symptom-free survival of medulloblastoma bearing mice significantly. The data shown represent six independent experiments.

Article Snippet: The human medulloblastoma cell lines Daoy (HTB 186), D283 Med (HTB185) and D341 Med (HTB-187) were obtained from American Type Culture Collection.

Techniques: In Vivo, Transplantation Assay