Journal: Journal of Neuroinflammation

Article Title: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology

doi: 10.1186/1742-2094-10-25

Figure Lengend Snippet: Antibodies tested in the study

Article Snippet: C5L2 N 1-23 , Rabbit polyclonal (Hycult) , + , +.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology

doi: 10.1186/1742-2094-10-25

Figure Lengend Snippet: C5L2 is present in neurons of control brain and neurofibrillary tangles of AD brain. Immunostaining with anti C5L2 antibody N1-23 shows labeling of a subset of neurons in frontal cortex (FC) ( A ) and hippocampus ( B ) of control cases and strong staining of tangles (arrowheads) in frontal cortex ( C ) and hippocampus ( D ) of AD cases. Scale bar: 50 um. Arrow in insert ( D ) shows neuropil threads. C5L2 N1-23 antibody immunostaining in hippocampus of an AD case ( E ) disappears after antibody preabsorption with the peptide ( F ). Scale bars: A,B,C,D (and insert): 50 um; E,F: 100 um. AD, Alzheimer’s disease.

Article Snippet: C5L2 N 1-23 , Rabbit polyclonal (Hycult) , + , +.

Techniques: Immunostaining, Labeling, Staining

Journal: Journal of Neuroinflammation

Article Title: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology

doi: 10.1186/1742-2094-10-25

Figure Lengend Snippet: C5L2 antibodies label dystrophic neurites associated with plaques as well as neurofibrillary tangles. Representative pictures of C5L2 staining using antibody N1-50 in FC of VD ( A ) and hippocampus of control ( B ) and VD ( C ) brain, and FC ( D ) or hippocampus of two AD cases ( E, F ). Arrowhead in D shows dystrophic neurites associated with plaques in cortex (DNP). Insert in E shows DNP in hippocampus. Scale bars: 50 um. AD, Alzheimer’s disease; FC, frontal cortex; VD, vascular dementia.

Article Snippet: C5L2 N 1-23 , Rabbit polyclonal (Hycult) , + , +.

Techniques: Staining

Journal: Journal of Neuroinflammation

Article Title: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology

doi: 10.1186/1742-2094-10-25

Figure Lengend Snippet: C5L2 and CD88 expression is upregulated in AD cases. A . Control and AD extracts from hippocampus subjected to SDS polyacrylamide gel and transferred to PVDF membranes were probed sequentially with anti human C5L2 antibody (N1-23 epitope) upper lane, anti human CD88 (Abcam, C terminal epitope) (middle lane) and anti ß actin (lower lane). B . Western blot of hippocampus lysates of AD case probed with C5L2 N1-23 antibody with (left lane) and without (right lane) preadsorption of the antibody with the N1-23 peptide. The 37 k Mr band is eliminated by preadsorption. C . Western blot of hippocampus homogenates of control and AD cases (from the Rush Religious Order Study) using Abcam polyclonal antibody against aa248-311of C5L2 and ß actin antibody for further densitometric quantification. D . Densitometric quantification of C5L2 immunoblots. Bars show average of ratio of C5L2/ßActin +/− SE from Figure which are AD (n = 5) 0.31 +/−0.05 and C (n = 4) 0.12+/− 0.03, P <0.01 (left) or from Figure and data not shown AD (n = 12) 1.05+/−0.05 and C (n = 12) 0.72+/− 0.03, P <0.01 (right). AD, Alzheimer’s disease; C, control; n, number; PVDF, polyvinylidene difluoride.

Article Snippet: C5L2 N 1-23 , Rabbit polyclonal (Hycult) , + , +.

Techniques: Expressing, Western Blot

Journal: Journal of Neuroinflammation

Article Title: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology

doi: 10.1186/1742-2094-10-25

Figure Lengend Snippet: C5L2 and CD88 colocalize with markers of hyperphosphorylated tau and can be expressed in the same neurofibrillary tangles. Confocal pictures of hippocampus of AD cases showing colocalization of ( A ) C5L2 (Abcam, anti N1-50, red) and AT8 (green), ( B ) CD88 (Novus anti C300-350, red) and AT8 (green), and ( C ) CD88 (Novus anti C300-350, green) and C5L2 (anti N1-50 red) colocalize in tangles. Scale bar: 50 um. AD, Alzheimer’s disease.

Article Snippet: C5L2 N 1-23 , Rabbit polyclonal (Hycult) , + , +.

Techniques: