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Journal: Frontiers in Immunology
Article Title: Complement Genetic Variants and FH Desialylation in S. pneumoniae -Haemolytic Uraemic Syndrome
doi: 10.3389/fimmu.2021.641656
Figure Lengend Snippet: Desialylation of FHR-1 and FHR-3 in patient H837 by 2D-WB analysis. FHR1 and FHR3 partially purified from sp1 and sp2 from patient H837 and from a control individual were subjected to 2D-electrophoresis and Western-blot with polyclonal antibodies. The characteristic ladder of FHR-3 spots (each having different Mw and isoelectric point) appeared as a few basic spots of lower Mw in the patient sample obtained 1 day after SP-HUS diagnosis (H837 sp1), and was recovered in the sample drawn 3 months later (H837 sp2). A similar situation applies to FHR-1. The small boxes within the gel images indicate the neuraminidase activity detected in samples sp1 and sp2 from patient H837.
Article Snippet: The anti-FH monoclonal antibodies (mAb) OX24 and C18 were from ThermoFisher (MA170057 and GAU0180302); the
Techniques: Purification, Two-Dimensional Gel Electrophoresis, Western Blot, Activity Assay

Journal: Frontiers in Immunology
Article Title: Complement Genetic Variants and FH Desialylation in S. pneumoniae -Haemolytic Uraemic Syndrome
doi: 10.3389/fimmu.2021.641656
Figure Lengend Snippet: Desialylated FH/FHRs in samples from Hungarian SP-HUS patients. WB analyses of FH/FHRs in two plasma samples (sp1 and sp2) from four Hungarian patients. Time between disease onset and extraction date was as follows: HUN156 (10 days and 11 months); HUN816 (9 days and 2 months); HUN1869 (5 days and 2 months), this patient is homozygous for FHR-3 and FHR-1 deficiency; HUN2638 (23 days and 43 days). WB analysis of human Transferrin (Mw 77 kDa) in sp1 and sp2 samples from patients H837, HUN156, and HUN 1869 is shown at the bottom inset.
Article Snippet: The anti-FH monoclonal antibodies (mAb) OX24 and C18 were from ThermoFisher (MA170057 and GAU0180302); the
Techniques: