Journal: Journal of Applied Physiology

Article Title: Inflammatory responses to acute elevations of carbon dioxide in mice

doi: 10.1152/japplphysiol.00343.2017

Figure Lengend Snippet: Flow cytometry agent suppliers

Article Snippet: Anti-mouse MPO PE , Hycult Biotechnology (HM1051PE-100).

Techniques: Flow Cytometry, Activation Assay

Journal: Traffic (Copenhagen, Denmark)

Article Title: The Rab27a Effectors JFC1/Slp1 and Munc13-4 Regulate Exocytosis of Neutrophil Granules

doi: 10.1111/j.1600-0854.2008.00838.x

Figure Lengend Snippet: Munc13-4-deficient neutrophils show impaired mobilization of secretory organelles. A–C) Neutrophils were isolated from the peritoneum of wild-type (WT) or Munc13-4Jinx/Jinx(JINX) mice 4 h after intraperitoneal thioglycolate injections. The cells were exposed to HKLM or incubated at 37°C in the absence of stimuli (NS, non-stimulated). Next, cell surfaces were labeled with anti-MPO, anti-LAMP-2 or anti-IL10R. The cells were fixed and analyzed by flow cytometry. D) Exocytosis of readily mobilizable organelles was analyzed by following the surface expression of CD11b in PMA-stimulated neutrophils. Each symbol represents the result obtained using an individual mouse. *p < 0.05, **p < 0.01.

Article Snippet: We also used FITC-conjugated anti-mouse MPO (HyCult biotechnology), Alexa Fluor 647-conjugated anti-mouse LAMP-2 (CD107b) (Biolegend) and anti-human anti-MPO (HyCult biotechnology).

Techniques: Isolation, Incubation, Labeling, Flow Cytometry, Expressing