Journal: Acta Neuropathologica Communications

Article Title: Targeted deletions of complement lectin pathway genes improve outcome in traumatic brain injury, with MASP-2 playing a major role

doi: 10.1186/s40478-020-01041-1

Figure Lengend Snippet: Summary table

Article Snippet: The brain coronal sections were incubated overnight at 4 °C with primary monoclonal antibody anti-mouse MBL-C (1 µg/ml; Hycult Biotechnology, Uden, The Netherlands) followed by a secondary biotinylated antibody against rat IgG.

Techniques:

Journal: Acta Neuropathologica Communications

Article Title: Targeted deletions of complement lectin pathway genes improve outcome in traumatic brain injury, with MASP-2 playing a major role

doi: 10.1186/s40478-020-01041-1

Figure Lengend Snippet: Experimental design. a WT or KOs mice (including: MASP-2 −/− , MBL −/− , FCN-A −/− , CL-11 −/− , MBL-C −/− , MASP-1/3 −/− and MBL-A −/− ) were subjected to TBI or sham injury. Sensorimotor deficits were assessed weekly by neuroscore and beam walk test. The sum of 4-week performances of each mouse genotype has been used to calculate the health score. b MBL brain presence and residual LP activity in plasma was assed in MASP-2 −/− and WT TBI mice 30′ after surgery. Histopathological analysis was done for MASP-2 −/− and WT mice at 6 weeks after TBI

Article Snippet: The brain coronal sections were incubated overnight at 4 °C with primary monoclonal antibody anti-mouse MBL-C (1 µg/ml; Hycult Biotechnology, Uden, The Netherlands) followed by a secondary biotinylated antibody against rat IgG.

Techniques: Activity Assay

Journal: Acta Neuropathologica Communications

Article Title: Targeted deletions of complement lectin pathway genes improve outcome in traumatic brain injury, with MASP-2 playing a major role

doi: 10.1186/s40478-020-01041-1

Figure Lengend Snippet: Brain MBL-C deposition and plasmatic LP activation 30′ after TBI in WT or MASP-2 −/− mice. a Representative low-magnification images of MBL-C immunolabeling at 30′ after TBI or sham surgery (the cortical edge is outlined in yellow). MBL-C quantification was done over an area of 350 µm from the contusion edge (Fig. ). Scale bars 50 μm. b MBL-C deposition in brains of MASP-2 −/− mice was similar to that of WT. Data is shown as a scatter dot plot, line at mean ± SEM ( n = 2–4). Two-way Anova followed by Sidak’s post hoc test, ** p <0.01 compared with Sham MASP-2 −/− , *** p < 0.001 compared with Sham WT. c In vitro assay for MBL-driven LP activation on mannan—plasma from MASP-2 −/− lack C4 convertase activity, resulting in minimal C4b deposition compared to WT mice. The data is shown as a scatter dot plot, line at mean ± SEM ( n = 2–4), Two-way Anova followed by Sidak’s post hoc test, *** p <0.001 compared with Sham or TBI WT

Article Snippet: The brain coronal sections were incubated overnight at 4 °C with primary monoclonal antibody anti-mouse MBL-C (1 µg/ml; Hycult Biotechnology, Uden, The Netherlands) followed by a secondary biotinylated antibody against rat IgG.

Techniques: Activation Assay, Immunolabeling, In Vitro, Activity Assay