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  • 94
    Sino Biological slc2a1 cdna orf clone human c his tag
    Slc2a1 Cdna Orf Clone Human C His Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slc2a1 cdna orf clone human c his tag/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    slc2a1 cdna orf clone human c his tag - by Bioz Stars, 2021-05
    94/100 stars
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    93
    Sino Biological kat2a cdna plasmids
    CPT1A binds to and succinylates LDHA at K222. a and b <t>KAT2A</t> does not bind to LDHA or its mutant K222E. AGS cells were transfected with Flag-KAT2A in combination with HA-LDHA or HA-K222E plasmid. KAT2A fusion protein was pulled down by Flag antibody, LDHA or K222E was detected using HA antibody. c - e CPT1A interacts with LDHA and its mutant K222E in AGS and 293 T cells. Cells were transfected with Flag-CPT1A in combination with HA-LDHA ( c , d ) or HA-K222E ( e ). The co-IP assay was performed similarly as above ( a , b ). f and g CPT1A promotes the succinylation of LDHA ( f ), but not of its mutant K222E ( g ). AGS cells were transfected with the indicated plasmids. Then, HA-LDHA was immunoprecipitated and its succinylation analyzed using either pan-succinylation or K222suc-specific antibody. h and i Knockdown efficiency of three CPT1A shRNAs in AGS cells was determined by western blot ( h ). CPT1A deficiency decreased the succinylation of LDHA ( i )
    Kat2a Cdna Plasmids, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kat2a cdna plasmids/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kat2a cdna plasmids - by Bioz Stars, 2021-05
    93/100 stars
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    94
    Sino Biological overexpression
    CPT1A binds to and succinylates LDHA at K222. a and b <t>KAT2A</t> does not bind to LDHA or its mutant K222E. AGS cells were transfected with Flag-KAT2A in combination with HA-LDHA or HA-K222E plasmid. KAT2A fusion protein was pulled down by Flag antibody, LDHA or K222E was detected using HA antibody. c - e CPT1A interacts with LDHA and its mutant K222E in AGS and 293 T cells. Cells were transfected with Flag-CPT1A in combination with HA-LDHA ( c , d ) or HA-K222E ( e ). The co-IP assay was performed similarly as above ( a , b ). f and g CPT1A promotes the succinylation of LDHA ( f ), but not of its mutant K222E ( g ). AGS cells were transfected with the indicated plasmids. Then, HA-LDHA was immunoprecipitated and its succinylation analyzed using either pan-succinylation or K222suc-specific antibody. h and i Knockdown efficiency of three CPT1A shRNAs in AGS cells was determined by western blot ( h ). CPT1A deficiency decreased the succinylation of LDHA ( i )
    Overexpression, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/overexpression/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    overexpression - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    80
    Sino Biological pcmv3 rrm2 flag
    CPT1A binds to and succinylates LDHA at K222. a and b <t>KAT2A</t> does not bind to LDHA or its mutant K222E. AGS cells were transfected with Flag-KAT2A in combination with HA-LDHA or HA-K222E plasmid. KAT2A fusion protein was pulled down by Flag antibody, LDHA or K222E was detected using HA antibody. c - e CPT1A interacts with LDHA and its mutant K222E in AGS and 293 T cells. Cells were transfected with Flag-CPT1A in combination with HA-LDHA ( c , d ) or HA-K222E ( e ). The co-IP assay was performed similarly as above ( a , b ). f and g CPT1A promotes the succinylation of LDHA ( f ), but not of its mutant K222E ( g ). AGS cells were transfected with the indicated plasmids. Then, HA-LDHA was immunoprecipitated and its succinylation analyzed using either pan-succinylation or K222suc-specific antibody. h and i Knockdown efficiency of three CPT1A shRNAs in AGS cells was determined by western blot ( h ). CPT1A deficiency decreased the succinylation of LDHA ( i )
    Pcmv3 Rrm2 Flag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv3 rrm2 flag/product/Sino Biological
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmv3 rrm2 flag - by Bioz Stars, 2021-05
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    Image Search Results


    CPT1A binds to and succinylates LDHA at K222. a and b KAT2A does not bind to LDHA or its mutant K222E. AGS cells were transfected with Flag-KAT2A in combination with HA-LDHA or HA-K222E plasmid. KAT2A fusion protein was pulled down by Flag antibody, LDHA or K222E was detected using HA antibody. c - e CPT1A interacts with LDHA and its mutant K222E in AGS and 293 T cells. Cells were transfected with Flag-CPT1A in combination with HA-LDHA ( c , d ) or HA-K222E ( e ). The co-IP assay was performed similarly as above ( a , b ). f and g CPT1A promotes the succinylation of LDHA ( f ), but not of its mutant K222E ( g ). AGS cells were transfected with the indicated plasmids. Then, HA-LDHA was immunoprecipitated and its succinylation analyzed using either pan-succinylation or K222suc-specific antibody. h and i Knockdown efficiency of three CPT1A shRNAs in AGS cells was determined by western blot ( h ). CPT1A deficiency decreased the succinylation of LDHA ( i )

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Lysine-222 succinylation reduces lysosomal degradation of lactate dehydrogenase a and is increased in gastric cancer

    doi: 10.1186/s13046-020-01681-0

    Figure Lengend Snippet: CPT1A binds to and succinylates LDHA at K222. a and b KAT2A does not bind to LDHA or its mutant K222E. AGS cells were transfected with Flag-KAT2A in combination with HA-LDHA or HA-K222E plasmid. KAT2A fusion protein was pulled down by Flag antibody, LDHA or K222E was detected using HA antibody. c - e CPT1A interacts with LDHA and its mutant K222E in AGS and 293 T cells. Cells were transfected with Flag-CPT1A in combination with HA-LDHA ( c , d ) or HA-K222E ( e ). The co-IP assay was performed similarly as above ( a , b ). f and g CPT1A promotes the succinylation of LDHA ( f ), but not of its mutant K222E ( g ). AGS cells were transfected with the indicated plasmids. Then, HA-LDHA was immunoprecipitated and its succinylation analyzed using either pan-succinylation or K222suc-specific antibody. h and i Knockdown efficiency of three CPT1A shRNAs in AGS cells was determined by western blot ( h ). CPT1A deficiency decreased the succinylation of LDHA ( i )

    Article Snippet: CPT1A and KAT2A cDNA plasmids were purchased from Sino Biological (Beijing, China) and subsequently cloned into the pRF-FLAG vector.

    Techniques: Mutagenesis, Transfection, Plasmid Preparation, Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot