HG15238-ACR Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Sino Biological clic1 cdna orf clone human c ofpspark tag
    The vesicular transfer of <t>CLIC1</t> requires cytosolic Ca 2+ increases in HMEC (A) Line scan images of cytosolic [Ca 2+ ] increases in Fluo-4 loaded HMEC exposed to the same amount of EVs collected from the same homotypic U87 culture. HMEC were pre-treated with 2-APB (50 μM; lower panel). Tridimensional histogram (F/F 0 vs space/time) are representative of 3 experiments. (B) Transfer of vesicular CLIC1 was blocked by 2-APB (50 μM). HMEC silenced by siRNA CLIC1 were exposed to EVs from U87 (n = 3). After 24h of incubation with EVs, HMEC were stained for CLIC1 (red) and nuclei (blue). (C) Expression of TRPM7 in HMEC. Homotypic HMEC were loaded (M) or not (E) with miR-5096 upon transfection of control siRNA or siRNA targeting TRPM7. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2; 80μg proteins/lane). (D) Spatially average Ca 2+ profile showing the dynamic change of Ca 2+ signals with time and induced by EVs (applied at the beginning of the records) then EGF (10 ng/ml) applied at the time indicated by arrow. Cytosolic Ca 2+ store depletion was performed by the addition of thapsigargin (TSG, 5μM) at the end of recordings. Values are means of fluorescent ratio F/F 0 ± SD; n = 3. (E) Silencing TRPM7 in HMEC reduced the Ca 2+ signal induced by EVs collected from homotypic U87 for 48h. (F) Control and silenced TRPM7 HMEC were exposed to EVs and stained for CLIC1 (red) after 24 h of culture (representative of 3 experiments). (G) Expression of TRPM7 in homotypic HMEC upon transfection of control siRNA or siRNA targeting Kir4.1 [ 18 ]. HMEC were exposed to the effluent (soluble fraction) from homotypic U87. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2).
    Clic1 Cdna Orf Clone Human C Ofpspark Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clic1 cdna orf clone human c ofpspark tag/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    clic1 cdna orf clone human c ofpspark tag - by Bioz Stars, 2021-04
    91/100 stars
      Buy from Supplier

    86
    Sino Biological c ofpspark
    The vesicular transfer of <t>CLIC1</t> requires cytosolic Ca 2+ increases in HMEC (A) Line scan images of cytosolic [Ca 2+ ] increases in Fluo-4 loaded HMEC exposed to the same amount of EVs collected from the same homotypic U87 culture. HMEC were pre-treated with 2-APB (50 μM; lower panel). Tridimensional histogram (F/F 0 vs space/time) are representative of 3 experiments. (B) Transfer of vesicular CLIC1 was blocked by 2-APB (50 μM). HMEC silenced by siRNA CLIC1 were exposed to EVs from U87 (n = 3). After 24h of incubation with EVs, HMEC were stained for CLIC1 (red) and nuclei (blue). (C) Expression of TRPM7 in HMEC. Homotypic HMEC were loaded (M) or not (E) with miR-5096 upon transfection of control siRNA or siRNA targeting TRPM7. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2; 80μg proteins/lane). (D) Spatially average Ca 2+ profile showing the dynamic change of Ca 2+ signals with time and induced by EVs (applied at the beginning of the records) then EGF (10 ng/ml) applied at the time indicated by arrow. Cytosolic Ca 2+ store depletion was performed by the addition of thapsigargin (TSG, 5μM) at the end of recordings. Values are means of fluorescent ratio F/F 0 ± SD; n = 3. (E) Silencing TRPM7 in HMEC reduced the Ca 2+ signal induced by EVs collected from homotypic U87 for 48h. (F) Control and silenced TRPM7 HMEC were exposed to EVs and stained for CLIC1 (red) after 24 h of culture (representative of 3 experiments). (G) Expression of TRPM7 in homotypic HMEC upon transfection of control siRNA or siRNA targeting Kir4.1 [ 18 ]. HMEC were exposed to the effluent (soluble fraction) from homotypic U87. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2).
    C Ofpspark, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c ofpspark/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c ofpspark - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    The vesicular transfer of CLIC1 requires cytosolic Ca 2+ increases in HMEC (A) Line scan images of cytosolic [Ca 2+ ] increases in Fluo-4 loaded HMEC exposed to the same amount of EVs collected from the same homotypic U87 culture. HMEC were pre-treated with 2-APB (50 μM; lower panel). Tridimensional histogram (F/F 0 vs space/time) are representative of 3 experiments. (B) Transfer of vesicular CLIC1 was blocked by 2-APB (50 μM). HMEC silenced by siRNA CLIC1 were exposed to EVs from U87 (n = 3). After 24h of incubation with EVs, HMEC were stained for CLIC1 (red) and nuclei (blue). (C) Expression of TRPM7 in HMEC. Homotypic HMEC were loaded (M) or not (E) with miR-5096 upon transfection of control siRNA or siRNA targeting TRPM7. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2; 80μg proteins/lane). (D) Spatially average Ca 2+ profile showing the dynamic change of Ca 2+ signals with time and induced by EVs (applied at the beginning of the records) then EGF (10 ng/ml) applied at the time indicated by arrow. Cytosolic Ca 2+ store depletion was performed by the addition of thapsigargin (TSG, 5μM) at the end of recordings. Values are means of fluorescent ratio F/F 0 ± SD; n = 3. (E) Silencing TRPM7 in HMEC reduced the Ca 2+ signal induced by EVs collected from homotypic U87 for 48h. (F) Control and silenced TRPM7 HMEC were exposed to EVs and stained for CLIC1 (red) after 24 h of culture (representative of 3 experiments). (G) Expression of TRPM7 in homotypic HMEC upon transfection of control siRNA or siRNA targeting Kir4.1 [ 18 ]. HMEC were exposed to the effluent (soluble fraction) from homotypic U87. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2).

    Journal: Oncotarget

    Article Title: The vesicular transfer of CLIC1 from glioblastoma to microvascular endothelial cells requires TRPM7

    doi: 10.18632/oncotarget.26048

    Figure Lengend Snippet: The vesicular transfer of CLIC1 requires cytosolic Ca 2+ increases in HMEC (A) Line scan images of cytosolic [Ca 2+ ] increases in Fluo-4 loaded HMEC exposed to the same amount of EVs collected from the same homotypic U87 culture. HMEC were pre-treated with 2-APB (50 μM; lower panel). Tridimensional histogram (F/F 0 vs space/time) are representative of 3 experiments. (B) Transfer of vesicular CLIC1 was blocked by 2-APB (50 μM). HMEC silenced by siRNA CLIC1 were exposed to EVs from U87 (n = 3). After 24h of incubation with EVs, HMEC were stained for CLIC1 (red) and nuclei (blue). (C) Expression of TRPM7 in HMEC. Homotypic HMEC were loaded (M) or not (E) with miR-5096 upon transfection of control siRNA or siRNA targeting TRPM7. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2; 80μg proteins/lane). (D) Spatially average Ca 2+ profile showing the dynamic change of Ca 2+ signals with time and induced by EVs (applied at the beginning of the records) then EGF (10 ng/ml) applied at the time indicated by arrow. Cytosolic Ca 2+ store depletion was performed by the addition of thapsigargin (TSG, 5μM) at the end of recordings. Values are means of fluorescent ratio F/F 0 ± SD; n = 3. (E) Silencing TRPM7 in HMEC reduced the Ca 2+ signal induced by EVs collected from homotypic U87 for 48h. (F) Control and silenced TRPM7 HMEC were exposed to EVs and stained for CLIC1 (red) after 24 h of culture (representative of 3 experiments). (G) Expression of TRPM7 in homotypic HMEC upon transfection of control siRNA or siRNA targeting Kir4.1 [ 18 ]. HMEC were exposed to the effluent (soluble fraction) from homotypic U87. Numbers indicate mean OD values of TRPM7 related to β-actin (± SD; n = 2).

    Article Snippet: To overexpress fluorescent CLIC1 proteins, we transfected U87 cells with the human CLIC1/NCC27 gene ORF cDNA clone expression plasmid, C-OFPSpark (HG15242-ACR, Sino Biological Inc.; purchased from Interchim, Montluçon, Fr).

    Techniques: Incubation, Staining, Expressing, Transfection

    Active CLIC1 protein is transferred via vesicles from GBM to endothelial cells Immunoblot analysis of CLIC1 in whole cell lysates (WCL) from homotypic cultures of U87 and HMEC, 48 h after loading. Untreated cells were used as control (Co). Cells were loaded empty (E) or with 30nM miR5096 mimic (M) or inhibitor (I). β-actin as loading control (60μg proteins/lane). Numbers indicate mean values of optical densities (OD) of CLIC1 relative to β–actin (± SD; P > 0.05 vs Co; n = 3). (B) CLIC1 increased in HMEC after 24 h of incubation with EVs. Cell-conditioned media were collected from homotypic U87 miR-loaded (M) or not (E), 48h after loading. HMEC were exposed to EVs or effluent (soluble fraction) separated from U87-conditioned media. Numbers indicate mean OD values of CLIC1 relative to β-actin (± SD; * P

    Journal: Oncotarget

    Article Title: The vesicular transfer of CLIC1 from glioblastoma to microvascular endothelial cells requires TRPM7

    doi: 10.18632/oncotarget.26048

    Figure Lengend Snippet: Active CLIC1 protein is transferred via vesicles from GBM to endothelial cells Immunoblot analysis of CLIC1 in whole cell lysates (WCL) from homotypic cultures of U87 and HMEC, 48 h after loading. Untreated cells were used as control (Co). Cells were loaded empty (E) or with 30nM miR5096 mimic (M) or inhibitor (I). β-actin as loading control (60μg proteins/lane). Numbers indicate mean values of optical densities (OD) of CLIC1 relative to β–actin (± SD; P > 0.05 vs Co; n = 3). (B) CLIC1 increased in HMEC after 24 h of incubation with EVs. Cell-conditioned media were collected from homotypic U87 miR-loaded (M) or not (E), 48h after loading. HMEC were exposed to EVs or effluent (soluble fraction) separated from U87-conditioned media. Numbers indicate mean OD values of CLIC1 relative to β-actin (± SD; * P

    Article Snippet: To overexpress fluorescent CLIC1 proteins, we transfected U87 cells with the human CLIC1/NCC27 gene ORF cDNA clone expression plasmid, C-OFPSpark (HG15242-ACR, Sino Biological Inc.; purchased from Interchim, Montluçon, Fr).

    Techniques: Incubation