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  • 91
    Sino Biological pcmv3 n ofpspark
    Pcmv3 N Ofpspark, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv3 n ofpspark/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmv3 n ofpspark - by Bioz Stars, 2021-04
    91/100 stars
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    93
    Sino Biological sap18
    Binding of A671 and related compounds to <t>SAP18.</t> a Western blotting for SAP18 following pull-down of Sepharose 6B beads loaded with indicated compounds. Sepharose 6B beads without compound used as control. b Binding of A671 to recombinant SAP18 in pull-down assays. c Western blot for SAP18 expression 12 h after treatment with indicated concentrations of A670-A672 compounds. d , e Expression of SIRT3 in HEL cells treated for 24 h with indicated concentrations of A670 ( d ) or A672 ( e ). f , g Growth curves of HEL cells treated for indicated periods and concentrations of A670 ( f ) or A672 ( g ). h Pull-down assay for binding of A611 to SAP18 and SIRT3 in HEL cells. The input used as a positive control. P
    Sap18, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sap18/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sap18 - by Bioz Stars, 2021-04
    93/100 stars
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    86
    Genecopoeia pez m32 ecfp n impdh1
    Binding of A671 and related compounds to <t>SAP18.</t> a Western blotting for SAP18 following pull-down of Sepharose 6B beads loaded with indicated compounds. Sepharose 6B beads without compound used as control. b Binding of A671 to recombinant SAP18 in pull-down assays. c Western blot for SAP18 expression 12 h after treatment with indicated concentrations of A670-A672 compounds. d , e Expression of SIRT3 in HEL cells treated for 24 h with indicated concentrations of A670 ( d ) or A672 ( e ). f , g Growth curves of HEL cells treated for indicated periods and concentrations of A670 ( f ) or A672 ( g ). h Pull-down assay for binding of A611 to SAP18 and SIRT3 in HEL cells. The input used as a positive control. P
    Pez M32 Ecfp N Impdh1, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pez m32 ecfp n impdh1/product/Genecopoeia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pez m32 ecfp n impdh1 - by Bioz Stars, 2021-04
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    86
    Genecopoeia overexpression
    Binding of A671 and related compounds to <t>SAP18.</t> a Western blotting for SAP18 following pull-down of Sepharose 6B beads loaded with indicated compounds. Sepharose 6B beads without compound used as control. b Binding of A671 to recombinant SAP18 in pull-down assays. c Western blot for SAP18 expression 12 h after treatment with indicated concentrations of A670-A672 compounds. d , e Expression of SIRT3 in HEL cells treated for 24 h with indicated concentrations of A670 ( d ) or A672 ( e ). f , g Growth curves of HEL cells treated for indicated periods and concentrations of A670 ( f ) or A672 ( g ). h Pull-down assay for binding of A611 to SAP18 and SIRT3 in HEL cells. The input used as a positive control. P
    Overexpression, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/overexpression/product/Genecopoeia
    Average 86 stars, based on 1 article reviews
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    Image Search Results


    Binding of A671 and related compounds to SAP18. a Western blotting for SAP18 following pull-down of Sepharose 6B beads loaded with indicated compounds. Sepharose 6B beads without compound used as control. b Binding of A671 to recombinant SAP18 in pull-down assays. c Western blot for SAP18 expression 12 h after treatment with indicated concentrations of A670-A672 compounds. d , e Expression of SIRT3 in HEL cells treated for 24 h with indicated concentrations of A670 ( d ) or A672 ( e ). f , g Growth curves of HEL cells treated for indicated periods and concentrations of A670 ( f ) or A672 ( g ). h Pull-down assay for binding of A611 to SAP18 and SIRT3 in HEL cells. The input used as a positive control. P

    Journal: Communications Biology

    Article Title: A C21-steroidal derivative suppresses T-cell lymphoma in mice by inhibiting SIRT3 via SAP18-SIN3

    doi: 10.1038/s42003-020-01458-3

    Figure Lengend Snippet: Binding of A671 and related compounds to SAP18. a Western blotting for SAP18 following pull-down of Sepharose 6B beads loaded with indicated compounds. Sepharose 6B beads without compound used as control. b Binding of A671 to recombinant SAP18 in pull-down assays. c Western blot for SAP18 expression 12 h after treatment with indicated concentrations of A670-A672 compounds. d , e Expression of SIRT3 in HEL cells treated for 24 h with indicated concentrations of A670 ( d ) or A672 ( e ). f , g Growth curves of HEL cells treated for indicated periods and concentrations of A670 ( f ) or A672 ( g ). h Pull-down assay for binding of A611 to SAP18 and SIRT3 in HEL cells. The input used as a positive control. P

    Article Snippet: Similarly, SAP18 (pCMV3-SAP18-GFPSpark®; Sino Biological, China) overexpression studies conducted using Lipofectamine 3000 and hygromycin (250 µg/ml) used for cell selection.

    Techniques: Binding Assay, Western Blot, Recombinant, Expressing, Pull Down Assay, Positive Control

    Correlation between SAP18 and SIRT3 transcription in BCALL patients predicts drug response. a A model for the effect of A671 on SAP18/SIN3 and SIRT3, and its impact on leukemic cell survival. A671 binds and stabilizes SAP18, leading to activation of the SIN3 complex and transcriptional repression of SIRT3 expression, which culminates in apoptotic cell death. b A significant negative correlation ( P = 0.0001 by two tailed student t -test) of SIRT3 and SAP18 in patients with BCALL, and correlation ( r ) of 0.4. (Spearman’s rank analysis). c , d Overall survival rates for high SIRT3 and SAP18 expression in BCALL patients determined by Log-rank (Mantel-Cox) test.

    Journal: Communications Biology

    Article Title: A C21-steroidal derivative suppresses T-cell lymphoma in mice by inhibiting SIRT3 via SAP18-SIN3

    doi: 10.1038/s42003-020-01458-3

    Figure Lengend Snippet: Correlation between SAP18 and SIRT3 transcription in BCALL patients predicts drug response. a A model for the effect of A671 on SAP18/SIN3 and SIRT3, and its impact on leukemic cell survival. A671 binds and stabilizes SAP18, leading to activation of the SIN3 complex and transcriptional repression of SIRT3 expression, which culminates in apoptotic cell death. b A significant negative correlation ( P = 0.0001 by two tailed student t -test) of SIRT3 and SAP18 in patients with BCALL, and correlation ( r ) of 0.4. (Spearman’s rank analysis). c , d Overall survival rates for high SIRT3 and SAP18 expression in BCALL patients determined by Log-rank (Mantel-Cox) test.

    Article Snippet: Similarly, SAP18 (pCMV3-SAP18-GFPSpark®; Sino Biological, China) overexpression studies conducted using Lipofectamine 3000 and hygromycin (250 µg/ml) used for cell selection.

    Techniques: Activation Assay, Expressing, Two Tailed Test

    A671 binds SAP18 within the SIN3 suppressor complex to transcriptionally repress SIRT3 expression. a , b Efficient depletion of SAP18 in HEL cells using shRNA as determined by western blotting ( a ) and Q-RT-PCR ( b ). Untreated or scrambled transfected vector (Vector) cells used as control. c Depletion of SAP18 in HEL cells (Sh-SAP18) induces SIRT3 transcription. d Marginal effect of Sh-SAP18 on cell proliferation. e Survival rate of Sh-SAP18 and controls cells treated for 24 h with indicated concentrations of A671. f SIN3A transcription factor (TF) binding sites within the Sirtuin gene family promoters. DNA sequencing data derived from SIN3A chromatin immunoprecipitation (ChIP) for two cell types (GM12878, B-lymphocyte lymphoblastoid, and H1-hESC, human embryonic stem cells) obtained through the ENCODE database (for details see supplementary Fig. 8 ). Of the Sirtuin genes, SIN3A observed to bind within the promoters of all but SIRT7 . The greatest binding observed for SIRT3 was ~108% higher than SIRT1 in GM12878 cells and ~40% higher than SIRT2 in the H1-hESCs. g Expression of SAP18 in HEL cells treated with actinomycin D (10 μM) alone or in combination with A671 (0.5 μM) for 12 (top) or 24 h (bottom). GAPDH used as a loading control. h A three-dimensional view of the predicted interaction of A671 with SAP18. i Position of chemical interactions with the indicated amino acids within SAP18. j Predicted affinity properties of A671 to SAP18 and SIRT3. P

    Journal: Communications Biology

    Article Title: A C21-steroidal derivative suppresses T-cell lymphoma in mice by inhibiting SIRT3 via SAP18-SIN3

    doi: 10.1038/s42003-020-01458-3

    Figure Lengend Snippet: A671 binds SAP18 within the SIN3 suppressor complex to transcriptionally repress SIRT3 expression. a , b Efficient depletion of SAP18 in HEL cells using shRNA as determined by western blotting ( a ) and Q-RT-PCR ( b ). Untreated or scrambled transfected vector (Vector) cells used as control. c Depletion of SAP18 in HEL cells (Sh-SAP18) induces SIRT3 transcription. d Marginal effect of Sh-SAP18 on cell proliferation. e Survival rate of Sh-SAP18 and controls cells treated for 24 h with indicated concentrations of A671. f SIN3A transcription factor (TF) binding sites within the Sirtuin gene family promoters. DNA sequencing data derived from SIN3A chromatin immunoprecipitation (ChIP) for two cell types (GM12878, B-lymphocyte lymphoblastoid, and H1-hESC, human embryonic stem cells) obtained through the ENCODE database (for details see supplementary Fig. 8 ). Of the Sirtuin genes, SIN3A observed to bind within the promoters of all but SIRT7 . The greatest binding observed for SIRT3 was ~108% higher than SIRT1 in GM12878 cells and ~40% higher than SIRT2 in the H1-hESCs. g Expression of SAP18 in HEL cells treated with actinomycin D (10 μM) alone or in combination with A671 (0.5 μM) for 12 (top) or 24 h (bottom). GAPDH used as a loading control. h A three-dimensional view of the predicted interaction of A671 with SAP18. i Position of chemical interactions with the indicated amino acids within SAP18. j Predicted affinity properties of A671 to SAP18 and SIRT3. P

    Article Snippet: Similarly, SAP18 (pCMV3-SAP18-GFPSpark®; Sino Biological, China) overexpression studies conducted using Lipofectamine 3000 and hygromycin (250 µg/ml) used for cell selection.

    Techniques: Expressing, shRNA, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Plasmid Preparation, Binding Assay, DNA Sequencing, Derivative Assay, Chromatin Immunoprecipitation

    A671 is an HDAC type III inhibitor (HDACi-III) that attenuates SIRT3 by binding and activating SAP18. a HEL cells incubated for 30 min with the indicated concentrations of A671 followed by HDAC type I and IIA/IIB activity assays (Promega). b Trichostatin A used as a positive control. c HEL cells were treated with A671, followed by HDAC type III activity analysis. d Nicotinamide was used as a positive control. e – j Q-RT-PCR analysis of EL4 cells for the indicated genes following 12 h treatment with A671, DMSO, or medium. k , l Induction of SAP18 mRNA ( k ) and protein ( l ) levels by A671 in a dose-dependent manner. m , A671 treatment diminished SIRT3 transcription in a dose-dependent manner as determined by Q-RT-PCR. P

    Journal: Communications Biology

    Article Title: A C21-steroidal derivative suppresses T-cell lymphoma in mice by inhibiting SIRT3 via SAP18-SIN3

    doi: 10.1038/s42003-020-01458-3

    Figure Lengend Snippet: A671 is an HDAC type III inhibitor (HDACi-III) that attenuates SIRT3 by binding and activating SAP18. a HEL cells incubated for 30 min with the indicated concentrations of A671 followed by HDAC type I and IIA/IIB activity assays (Promega). b Trichostatin A used as a positive control. c HEL cells were treated with A671, followed by HDAC type III activity analysis. d Nicotinamide was used as a positive control. e – j Q-RT-PCR analysis of EL4 cells for the indicated genes following 12 h treatment with A671, DMSO, or medium. k , l Induction of SAP18 mRNA ( k ) and protein ( l ) levels by A671 in a dose-dependent manner. m , A671 treatment diminished SIRT3 transcription in a dose-dependent manner as determined by Q-RT-PCR. P

    Article Snippet: Similarly, SAP18 (pCMV3-SAP18-GFPSpark®; Sino Biological, China) overexpression studies conducted using Lipofectamine 3000 and hygromycin (250 µg/ml) used for cell selection.

    Techniques: Binding Assay, Incubation, Activity Assay, Positive Control, Reverse Transcription Polymerase Chain Reaction