HG10356-CM Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Sino Biological survivin plasmid
    Archazolid A decreases the anti-apoptotic protein <t>survivin</t> and interferes with the cell cycle in leukemic cells A. Archazolid A decreases the anti-apoptotic protein survivin. Immunoblots from Jurkat cells treated with Archazolid A (Arch, 10 nM, left panel) or DBZ (50 μM, right panel) for the indicated times and probed with antibodies for survivin, XIAP, IAP1, and IAP2 are shown. Immunoblots for tubulin indicate equal loading. n = 3. Bar graphs display the quantitative evaluation of survivin expression. B. Immunostainings for survivin (green) and actin (red) after treatment with/without Archazolid A (Arch, 10 nM, 24h) and DBZ (50 μM, 24h) is shown. Scale bar 10 μm. C. Archazolid A (Arch) and DBZ do not interfere with survivin mRNA expression. Survivin mRNA levels from Jurkat cells treated with Archazolid A (1 and 10 nM) and DBZ (50 μM) for 24h are shown. Not significant (ns), Archazolid A: One-Way ANOVA, DBZ: paired t -test, n = 3. D. Archazolid A (Arch) induces S-phase cell cycle arrest of Jurkat cells. Cell cyle analysis and apoptosis measurement after aphidicolin synchronization (24h) and subsequent treatment with Archazolid A for indicated times is shown. Control cells (untreated, Archazolid A 0 nM) are indicated in red, Archazolid A (Arch, 10 nM) treated cells are indicated in blue. One representative out of three independent experiments is shown.
    Survivin Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/survivin plasmid/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    survivin plasmid - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Archazolid A decreases the anti-apoptotic protein survivin and interferes with the cell cycle in leukemic cells A. Archazolid A decreases the anti-apoptotic protein survivin. Immunoblots from Jurkat cells treated with Archazolid A (Arch, 10 nM, left panel) or DBZ (50 μM, right panel) for the indicated times and probed with antibodies for survivin, XIAP, IAP1, and IAP2 are shown. Immunoblots for tubulin indicate equal loading. n = 3. Bar graphs display the quantitative evaluation of survivin expression. B. Immunostainings for survivin (green) and actin (red) after treatment with/without Archazolid A (Arch, 10 nM, 24h) and DBZ (50 μM, 24h) is shown. Scale bar 10 μm. C. Archazolid A (Arch) and DBZ do not interfere with survivin mRNA expression. Survivin mRNA levels from Jurkat cells treated with Archazolid A (1 and 10 nM) and DBZ (50 μM) for 24h are shown. Not significant (ns), Archazolid A: One-Way ANOVA, DBZ: paired t -test, n = 3. D. Archazolid A (Arch) induces S-phase cell cycle arrest of Jurkat cells. Cell cyle analysis and apoptosis measurement after aphidicolin synchronization (24h) and subsequent treatment with Archazolid A for indicated times is shown. Control cells (untreated, Archazolid A 0 nM) are indicated in red, Archazolid A (Arch, 10 nM) treated cells are indicated in blue. One representative out of three independent experiments is shown.

    Journal: Oncotarget

    Article Title: Anti-leukemic effects of the V-ATPase inhibitor Archazolid A

    doi:

    Figure Lengend Snippet: Archazolid A decreases the anti-apoptotic protein survivin and interferes with the cell cycle in leukemic cells A. Archazolid A decreases the anti-apoptotic protein survivin. Immunoblots from Jurkat cells treated with Archazolid A (Arch, 10 nM, left panel) or DBZ (50 μM, right panel) for the indicated times and probed with antibodies for survivin, XIAP, IAP1, and IAP2 are shown. Immunoblots for tubulin indicate equal loading. n = 3. Bar graphs display the quantitative evaluation of survivin expression. B. Immunostainings for survivin (green) and actin (red) after treatment with/without Archazolid A (Arch, 10 nM, 24h) and DBZ (50 μM, 24h) is shown. Scale bar 10 μm. C. Archazolid A (Arch) and DBZ do not interfere with survivin mRNA expression. Survivin mRNA levels from Jurkat cells treated with Archazolid A (1 and 10 nM) and DBZ (50 μM) for 24h are shown. Not significant (ns), Archazolid A: One-Way ANOVA, DBZ: paired t -test, n = 3. D. Archazolid A (Arch) induces S-phase cell cycle arrest of Jurkat cells. Cell cyle analysis and apoptosis measurement after aphidicolin synchronization (24h) and subsequent treatment with Archazolid A for indicated times is shown. Control cells (untreated, Archazolid A 0 nM) are indicated in red, Archazolid A (Arch, 10 nM) treated cells are indicated in blue. One representative out of three independent experiments is shown.

    Article Snippet: Survivin plasmid was from Sino Biologicals (pCMV3-BIRC5-myc, HG10356-CM G09AU4M62).

    Techniques: Western Blot, Expressing

    Archazolid A interferes with the iron metabolism in leukemic cells A. Archazolid A increases Hif1α. Immunoblots show Hif1α levels of Jurkat cells with/without Archazolid A (Arch) treatment at indicated concentrations for 24h. Actin indicates equal loading. B. Immunostainings for Hif1α (green) and f-actin (red) after treatment with/without Archazolid A (Arch, 10 nM, 24h) is shown. Nuclei are labeled with Hoechst33342 (blue). Scale bar 7.5 μm. C. Archazolid A mediated Hif1α increase is abrogated by iron citrate. Immunoblots show Hif1α levels of Jurkat cells with/without Archazolid A (Arch) and iron citrate (FeCit) treatment at indicated concentrations for 24h. Actin indicates equal loading. n = 3. D. Inhibition of Notch by DBZ does not influence Hif1α. Immunoblots of Jurkat cells treated with DBZ and deferoxamine (DFO) at indicated concentrations for 24h for Hif1α and actin (loading control) are shown. E. Archazolid A mediated cell death is partially rescued by iron citrate. The graph shows cell death of Jurkat cells treated with/without Archazolid A (Arch) and iron citrate (FeCit) at indicated concentrations for 48 h. Mann Whitney test, ** p = 0.0022, n = 3. F. DFO induces cell death in Jurkat cells and is enhanced by Archazolid A. Nicoletti assay of cells treated with/without Archazolid A (Arch) and DFO at indicated concentrations for 48 h is shown. One-Way ANOVA, Tukey's post test, *** p ≤ 0.001, n = 3. G. Survivin is decreased by DFO which is enhanced by Archazolid A. Immunoblots for survivin and tubulin (loading control) from cells treated with/without DFO (100 μM) and Archazolid A (Arch, 10 nM) for 48h are shown; n = 3.

    Journal: Oncotarget

    Article Title: Anti-leukemic effects of the V-ATPase inhibitor Archazolid A

    doi:

    Figure Lengend Snippet: Archazolid A interferes with the iron metabolism in leukemic cells A. Archazolid A increases Hif1α. Immunoblots show Hif1α levels of Jurkat cells with/without Archazolid A (Arch) treatment at indicated concentrations for 24h. Actin indicates equal loading. B. Immunostainings for Hif1α (green) and f-actin (red) after treatment with/without Archazolid A (Arch, 10 nM, 24h) is shown. Nuclei are labeled with Hoechst33342 (blue). Scale bar 7.5 μm. C. Archazolid A mediated Hif1α increase is abrogated by iron citrate. Immunoblots show Hif1α levels of Jurkat cells with/without Archazolid A (Arch) and iron citrate (FeCit) treatment at indicated concentrations for 24h. Actin indicates equal loading. n = 3. D. Inhibition of Notch by DBZ does not influence Hif1α. Immunoblots of Jurkat cells treated with DBZ and deferoxamine (DFO) at indicated concentrations for 24h for Hif1α and actin (loading control) are shown. E. Archazolid A mediated cell death is partially rescued by iron citrate. The graph shows cell death of Jurkat cells treated with/without Archazolid A (Arch) and iron citrate (FeCit) at indicated concentrations for 48 h. Mann Whitney test, ** p = 0.0022, n = 3. F. DFO induces cell death in Jurkat cells and is enhanced by Archazolid A. Nicoletti assay of cells treated with/without Archazolid A (Arch) and DFO at indicated concentrations for 48 h is shown. One-Way ANOVA, Tukey's post test, *** p ≤ 0.001, n = 3. G. Survivin is decreased by DFO which is enhanced by Archazolid A. Immunoblots for survivin and tubulin (loading control) from cells treated with/without DFO (100 μM) and Archazolid A (Arch, 10 nM) for 48h are shown; n = 3.

    Article Snippet: Survivin plasmid was from Sino Biologicals (pCMV3-BIRC5-myc, HG10356-CM G09AU4M62).

    Techniques: Western Blot, Labeling, Inhibition, MANN-WHITNEY, Nicoletti Assay

    Archazolid A-induced apoptosis in PDX is in line with decreased levels of the anti-apoptotic protein survivin A. Upper panels show apoptosis rates (specific cell death) determined by PI exclusion staining of PDX leukemia samples treated with Archazolid A (Arch, 10 nM, 48 h). Lower panels display immunoblots from PDX cells from the same patients treated with Archazolid A (Arch, 10 nM, 24 h) and probed with antibodies for survivin. Immunoblots for actin indicate equal loading. B. Archazolid A mediated cell death is partially rescued by survivin overexpression. The graph shows early apoptosis (AnnexinV-positive and PI-negative cells) determined by AnnexinV/PI staining of Jurkat cells overexpressing either empty vector (ev) or survivin and treated with/without Archazolid A (Arch) at indicated concentrations for 48 h. One-Way ANOVA, Tukey, * p ≤ 0.05, *** p ≤ 0.001, n = 3. Immunoblots show overexpression of empty vector (ev) and survivin 24h after transfection; actin indicates equal loading.

    Journal: Oncotarget

    Article Title: Anti-leukemic effects of the V-ATPase inhibitor Archazolid A

    doi:

    Figure Lengend Snippet: Archazolid A-induced apoptosis in PDX is in line with decreased levels of the anti-apoptotic protein survivin A. Upper panels show apoptosis rates (specific cell death) determined by PI exclusion staining of PDX leukemia samples treated with Archazolid A (Arch, 10 nM, 48 h). Lower panels display immunoblots from PDX cells from the same patients treated with Archazolid A (Arch, 10 nM, 24 h) and probed with antibodies for survivin. Immunoblots for actin indicate equal loading. B. Archazolid A mediated cell death is partially rescued by survivin overexpression. The graph shows early apoptosis (AnnexinV-positive and PI-negative cells) determined by AnnexinV/PI staining of Jurkat cells overexpressing either empty vector (ev) or survivin and treated with/without Archazolid A (Arch) at indicated concentrations for 48 h. One-Way ANOVA, Tukey, * p ≤ 0.05, *** p ≤ 0.001, n = 3. Immunoblots show overexpression of empty vector (ev) and survivin 24h after transfection; actin indicates equal loading.

    Article Snippet: Survivin plasmid was from Sino Biologicals (pCMV3-BIRC5-myc, HG10356-CM G09AU4M62).

    Techniques: Staining, Western Blot, Over Expression, Plasmid Preparation, Transfection