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  • 91
    Sino Biological junctional adhesion molecule 1
    Effect of PECAM-1 on degranulation in CD177-negative neutrophils. a SHP-1 phosphorylation was detected in neutrophils incubated with platelet endothelial cell adhesion <t>molecule-1</t> (PECAM-1). b Effect of PECAM-1 on degranulation of CD177-negative and mixed neutrophils. Degranulation induced by proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA) was little influenced by preincubation with soluble PECAM-1 in CD177-negative neutrophils, but it was significantly inhibited by soluble PECAM-1 in mixed neutrophils. The level of lactoferrin was expressed as a percentage of control in each subset. The purity of the CD177-positive subset in mixed neutrophils was 72.7 ± 10.7% and the purity of CD177-negative neutrophils after selecting was 86.4 ± 8.5%. Bars represent mean ± SD of repeated measurements from four independent experiments. ** p
    Junctional Adhesion Molecule 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/junctional adhesion molecule 1/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    junctional adhesion molecule 1 - by Bioz Stars, 2021-04
    91/100 stars
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    99
    New England Biolabs kpni hf
    Effect of PECAM-1 on degranulation in CD177-negative neutrophils. a SHP-1 phosphorylation was detected in neutrophils incubated with platelet endothelial cell adhesion <t>molecule-1</t> (PECAM-1). b Effect of PECAM-1 on degranulation of CD177-negative and mixed neutrophils. Degranulation induced by proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA) was little influenced by preincubation with soluble PECAM-1 in CD177-negative neutrophils, but it was significantly inhibited by soluble PECAM-1 in mixed neutrophils. The level of lactoferrin was expressed as a percentage of control in each subset. The purity of the CD177-positive subset in mixed neutrophils was 72.7 ± 10.7% and the purity of CD177-negative neutrophils after selecting was 86.4 ± 8.5%. Bars represent mean ± SD of repeated measurements from four independent experiments. ** p
    Kpni Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpni hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kpni hf - by Bioz Stars, 2021-04
    99/100 stars
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    86
    Sino Biological comp cdna orf clone human c ha tag
    Effect of PECAM-1 on degranulation in CD177-negative neutrophils. a SHP-1 phosphorylation was detected in neutrophils incubated with platelet endothelial cell adhesion <t>molecule-1</t> (PECAM-1). b Effect of PECAM-1 on degranulation of CD177-negative and mixed neutrophils. Degranulation induced by proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA) was little influenced by preincubation with soluble PECAM-1 in CD177-negative neutrophils, but it was significantly inhibited by soluble PECAM-1 in mixed neutrophils. The level of lactoferrin was expressed as a percentage of control in each subset. The purity of the CD177-positive subset in mixed neutrophils was 72.7 ± 10.7% and the purity of CD177-negative neutrophils after selecting was 86.4 ± 8.5%. Bars represent mean ± SD of repeated measurements from four independent experiments. ** p
    Comp Cdna Orf Clone Human C Ha Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/comp cdna orf clone human c ha tag/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    comp cdna orf clone human c ha tag - by Bioz Stars, 2021-04
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    99
    Thermo Fisher opti mem
    Effect of PECAM-1 on degranulation in CD177-negative neutrophils. a SHP-1 phosphorylation was detected in neutrophils incubated with platelet endothelial cell adhesion <t>molecule-1</t> (PECAM-1). b Effect of PECAM-1 on degranulation of CD177-negative and mixed neutrophils. Degranulation induced by proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA) was little influenced by preincubation with soluble PECAM-1 in CD177-negative neutrophils, but it was significantly inhibited by soluble PECAM-1 in mixed neutrophils. The level of lactoferrin was expressed as a percentage of control in each subset. The purity of the CD177-positive subset in mixed neutrophils was 72.7 ± 10.7% and the purity of CD177-negative neutrophils after selecting was 86.4 ± 8.5%. Bars represent mean ± SD of repeated measurements from four independent experiments. ** p
    Opti Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opti mem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opti mem - by Bioz Stars, 2021-04
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    Image Search Results


    Effect of PECAM-1 on degranulation in CD177-negative neutrophils. a SHP-1 phosphorylation was detected in neutrophils incubated with platelet endothelial cell adhesion molecule-1 (PECAM-1). b Effect of PECAM-1 on degranulation of CD177-negative and mixed neutrophils. Degranulation induced by proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA) was little influenced by preincubation with soluble PECAM-1 in CD177-negative neutrophils, but it was significantly inhibited by soluble PECAM-1 in mixed neutrophils. The level of lactoferrin was expressed as a percentage of control in each subset. The purity of the CD177-positive subset in mixed neutrophils was 72.7 ± 10.7% and the purity of CD177-negative neutrophils after selecting was 86.4 ± 8.5%. Bars represent mean ± SD of repeated measurements from four independent experiments. ** p

    Journal: Arthritis Research & Therapy

    Article Title: Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA

    doi: 10.1186/s13075-018-1710-0

    Figure Lengend Snippet: Effect of PECAM-1 on degranulation in CD177-negative neutrophils. a SHP-1 phosphorylation was detected in neutrophils incubated with platelet endothelial cell adhesion molecule-1 (PECAM-1). b Effect of PECAM-1 on degranulation of CD177-negative and mixed neutrophils. Degranulation induced by proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA) was little influenced by preincubation with soluble PECAM-1 in CD177-negative neutrophils, but it was significantly inhibited by soluble PECAM-1 in mixed neutrophils. The level of lactoferrin was expressed as a percentage of control in each subset. The purity of the CD177-positive subset in mixed neutrophils was 72.7 ± 10.7% and the purity of CD177-negative neutrophils after selecting was 86.4 ± 8.5%. Bars represent mean ± SD of repeated measurements from four independent experiments. ** p

    Article Snippet: Recombinant PECAM-1, junctional adhesion molecule-1 (JAM-1), and CD177 were purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Incubation

    Downregulation of mPR3 after treating neutrophils with PECAM-1. a interaction between platelet endothelial cell adhesion molecule-1 (PECAM-1) and CD177 at various concentrations. b Representative histogram of the effect of PECAM-1 on membrane-bound proteinase-3 (mPR3) expression in a dose-dependent manner. c Incubation of TNF-α-primed neutrophils with PECAM-1 at 30 μg/ml significantly decreased mPR3 expression. Bars denote means ± SD of mPR3 expression (mean fluorescence intensity; MFI). d Incubation of TNF-α-primed neutrophils with PECAM-1 at 30 μg/ml significantly increased proteinase-3 (PR3) levels in the supernatant. Bars denote means ± SD of PR3 concentration (ng/ml). Neutrophils treated with phorbol myristate acetate (PMA) were employed as positive control. * p

    Journal: Arthritis Research & Therapy

    Article Title: Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA

    doi: 10.1186/s13075-018-1710-0

    Figure Lengend Snippet: Downregulation of mPR3 after treating neutrophils with PECAM-1. a interaction between platelet endothelial cell adhesion molecule-1 (PECAM-1) and CD177 at various concentrations. b Representative histogram of the effect of PECAM-1 on membrane-bound proteinase-3 (mPR3) expression in a dose-dependent manner. c Incubation of TNF-α-primed neutrophils with PECAM-1 at 30 μg/ml significantly decreased mPR3 expression. Bars denote means ± SD of mPR3 expression (mean fluorescence intensity; MFI). d Incubation of TNF-α-primed neutrophils with PECAM-1 at 30 μg/ml significantly increased proteinase-3 (PR3) levels in the supernatant. Bars denote means ± SD of PR3 concentration (ng/ml). Neutrophils treated with phorbol myristate acetate (PMA) were employed as positive control. * p

    Article Snippet: Recombinant PECAM-1, junctional adhesion molecule-1 (JAM-1), and CD177 were purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Expressing, Incubation, Fluorescence, Concentration Assay, Positive Control

    Platelet endothelial cell adhesion molecule-1 (PECAM-1) interaction with CD177 decreased GEnC activation and injury induced by patient-derived proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA). By preincubation with PECAM-1, the levels of soluble intercellular cell adhesion molecule-1 (sICAM-1) significantly decreased in the supernatants of GEnCs treated with neutrophil plus patient-derived PR3-ANCA-positive IgGs. Bars denote means ± SD of sICAM-1 concentration (pg/mL). * p

    Journal: Arthritis Research & Therapy

    Article Title: Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA

    doi: 10.1186/s13075-018-1710-0

    Figure Lengend Snippet: Platelet endothelial cell adhesion molecule-1 (PECAM-1) interaction with CD177 decreased GEnC activation and injury induced by patient-derived proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA). By preincubation with PECAM-1, the levels of soluble intercellular cell adhesion molecule-1 (sICAM-1) significantly decreased in the supernatants of GEnCs treated with neutrophil plus patient-derived PR3-ANCA-positive IgGs. Bars denote means ± SD of sICAM-1 concentration (pg/mL). * p

    Article Snippet: Recombinant PECAM-1, junctional adhesion molecule-1 (JAM-1), and CD177 were purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Activation Assay, Derivative Assay, Concentration Assay