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  • 93
    Sino Biological cd34
    The proportion of <t>CD34</t> + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Cd34, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Sino Biological tnf α
    MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under <t>TNF-α</t> treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value
    Tnf α, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Sino Biological comp cdna orf clone human c ha tag
    MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under <t>TNF-α</t> treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value
    Comp Cdna Orf Clone Human C Ha Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Sino Biological tnf alpha cdna orf clone in cloning vector human
    MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under <t>TNF-α</t> treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value
    Tnf Alpha Cdna Orf Clone In Cloning Vector Human, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Journal: Biochemistry and Biophysics Reports

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    doi: 10.1016/j.bbrep.2016.11.006

    Figure Lengend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Article Snippet: It was classified as binding to the class II epitope of CD34 .

    Techniques: Flow Cytometry, Incubation

    MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under TNF-α treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under TNF-α treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: After 2 h, the cells were treated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h (SW1353 cells) or 6 h (C28/I2 cells), and then the cells were harvested for further analysis.

    Techniques: Activity Assay, Transfection, Sequencing, Expressing, Western Blot, Negative Control, MANN-WHITNEY

    HS6ST2 could regulate the matrix degradation depending on the activity of p38 MAPK. a SW1353 cells were transfected by si -HS6ST2 or negative control (si-NC) with or without TNF-α for 24 h, and the protein expression of p-p38 and p38 was detected by western blotting. Asterisk (*): compared with the si-NC group. b Under stimulation with TNF-α, SW1353 cells were transfected by empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) under treatment with mimic miR-23b-3p, and p-p38 and total p38 were determined by western blotting. Asterisk (*): compared with the mimic NC group. c SW1353 cells were transfected with HS6ST2 siRNA with or without p38 MAPK inhibitor SB203580 (10 μM) after treatment of TNF-α, and the protein expression of MMP13 was determined by western blotting. Each relative expression of phosphorylation form was normalized by the total form. d Schematic representation of miR-23b-3p–HS6ST2 axis-mediated catabolic effects in human chondrocyte. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: HS6ST2 could regulate the matrix degradation depending on the activity of p38 MAPK. a SW1353 cells were transfected by si -HS6ST2 or negative control (si-NC) with or without TNF-α for 24 h, and the protein expression of p-p38 and p38 was detected by western blotting. Asterisk (*): compared with the si-NC group. b Under stimulation with TNF-α, SW1353 cells were transfected by empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) under treatment with mimic miR-23b-3p, and p-p38 and total p38 were determined by western blotting. Asterisk (*): compared with the mimic NC group. c SW1353 cells were transfected with HS6ST2 siRNA with or without p38 MAPK inhibitor SB203580 (10 μM) after treatment of TNF-α, and the protein expression of MMP13 was determined by western blotting. Each relative expression of phosphorylation form was normalized by the total form. d Schematic representation of miR-23b-3p–HS6ST2 axis-mediated catabolic effects in human chondrocyte. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: After 2 h, the cells were treated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h (SW1353 cells) or 6 h (C28/I2 cells), and then the cells were harvested for further analysis.

    Techniques: Activity Assay, Transfection, Negative Control, Expressing, Western Blot, Plasmid Preparation, MANN-WHITNEY

    MiR-23b-3p could enhance matrix degradation in human chondrocytes via regulating HS6ST2. a , b SW1353 cells transfected with 50 nM HS6ST2 siRNA mixture (containing three target sequences, si- HS6ST2 ) or negative control (si-NC) were stimulated by 10 ng/ml TNF-α for 24 h. Protein level of HS6ST2 and MMP13 were assayed by western blotting ( a ) and matrix content of chondrocytes was determined by toluidine blue staining ( b ). Asterisk (*): compared with the si-NC group. c , d SW1353 cells transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for 24 h were stimulated by TNF-α for another 24 h. The protein expression of MMP13 was assayed by western blotting ( c ) and matrix content of chondrocytes was determined by toluidine blue staining ( d ). Asterisk (*): compared with the FLAG-Ctrl group. e , f Under stimulation with TNF-α, SW1353 cells were treated with mimic NC or mimic miR-23b-3p for 24 h and then transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for another 24 h to observe the rescuing effect of mimic miR-23b-3p in MMP13 expression ( e ) and matrix content ( f ). Asterisk (*): compared with the mimic NC group. GAPDH was used as internal controls in western blotting detection. In toluidine blue staining results, scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Bars represent standard error of the mean (SEM) from three independent experiments. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: MiR-23b-3p could enhance matrix degradation in human chondrocytes via regulating HS6ST2. a , b SW1353 cells transfected with 50 nM HS6ST2 siRNA mixture (containing three target sequences, si- HS6ST2 ) or negative control (si-NC) were stimulated by 10 ng/ml TNF-α for 24 h. Protein level of HS6ST2 and MMP13 were assayed by western blotting ( a ) and matrix content of chondrocytes was determined by toluidine blue staining ( b ). Asterisk (*): compared with the si-NC group. c , d SW1353 cells transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for 24 h were stimulated by TNF-α for another 24 h. The protein expression of MMP13 was assayed by western blotting ( c ) and matrix content of chondrocytes was determined by toluidine blue staining ( d ). Asterisk (*): compared with the FLAG-Ctrl group. e , f Under stimulation with TNF-α, SW1353 cells were treated with mimic NC or mimic miR-23b-3p for 24 h and then transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for another 24 h to observe the rescuing effect of mimic miR-23b-3p in MMP13 expression ( e ) and matrix content ( f ). Asterisk (*): compared with the mimic NC group. GAPDH was used as internal controls in western blotting detection. In toluidine blue staining results, scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Bars represent standard error of the mean (SEM) from three independent experiments. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: After 2 h, the cells were treated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h (SW1353 cells) or 6 h (C28/I2 cells), and then the cells were harvested for further analysis.

    Techniques: Transfection, Negative Control, Western Blot, Staining, Plasmid Preparation, Expressing, MANN-WHITNEY

    TNF-α-treated chondrocytes increases matrix degradation regulated by miR-23b-3p. a Stem-loop RT-qPCR result of miR-23b-3p (left panel) and protein levels of HS6ST2 and MMP13 (right panel) were assayed in SW1353 cells under stimulation by 10 ng/ml TNF-α for 24 h. b , c Western blotting result of HS6ST2 and MMP13 protein in SW1353 cells transfected with 10 nM mimic miR-23b-3p ( b ) or 50 nM anti-miR-23b-3p sequence ( c ) and stimulated by 10 ng/ml TNF-α for 24 h. d , e Toluidine blue staining results of SW1353 cells transfected with 10 nM mimic miR-23b-3p ( d ) or 50 nM anti-miR-23b-3p sequence ( e ) and stimulated by 10 ng/ml TNF-α for 24 h. Scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Asterisk (*): compared with mimic NC or anti-NC group. U6 snRNA and GAPDH were used as internal controls in RT-qPCR for miRNA and western blotting detection, respectively. Bars represent standard error of the mean (SEM) from three independent experiments. One representative result and quantitative data from three independent western blotting and toluidine blue staining. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: TNF-α-treated chondrocytes increases matrix degradation regulated by miR-23b-3p. a Stem-loop RT-qPCR result of miR-23b-3p (left panel) and protein levels of HS6ST2 and MMP13 (right panel) were assayed in SW1353 cells under stimulation by 10 ng/ml TNF-α for 24 h. b , c Western blotting result of HS6ST2 and MMP13 protein in SW1353 cells transfected with 10 nM mimic miR-23b-3p ( b ) or 50 nM anti-miR-23b-3p sequence ( c ) and stimulated by 10 ng/ml TNF-α for 24 h. d , e Toluidine blue staining results of SW1353 cells transfected with 10 nM mimic miR-23b-3p ( d ) or 50 nM anti-miR-23b-3p sequence ( e ) and stimulated by 10 ng/ml TNF-α for 24 h. Scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Asterisk (*): compared with mimic NC or anti-NC group. U6 snRNA and GAPDH were used as internal controls in RT-qPCR for miRNA and western blotting detection, respectively. Bars represent standard error of the mean (SEM) from three independent experiments. One representative result and quantitative data from three independent western blotting and toluidine blue staining. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: After 2 h, the cells were treated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h (SW1353 cells) or 6 h (C28/I2 cells), and then the cells were harvested for further analysis.

    Techniques: Quantitative RT-PCR, Western Blot, Transfection, Sequencing, Staining, MANN-WHITNEY