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antimicrobial peptides human beta defensin 2  (Hycult Biotech)
90
Hycult Biotech antimicrobial peptides human beta defensin 2
B. clausii increases <t>HBD-2</t> and LL-37 expression in human enterocytes. Cells were exposed to B. clausii probiotic strains mix at different concentrations; B. clausii supernatant (Sup, dilution 1:100) or E. coli K12 (1 × 10 6 cells/mL) as control. Only the exposure to B. clausii strains was able to elicit a significant increase in HBD-2 ( A ) and LL-37 ( B ) production by human enterocytes. HBD 2, human beta defensin 2; LL-37, cathelicidin; NT, untreated. *p < 0.05 vs NT; **p < 0.001 vs NT.
Antimicrobial Peptides Human Beta Defensin 2, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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B. clausii increases HBD-2 and LL-37 expression in human enterocytes. Cells were exposed to B. clausii probiotic strains mix at different concentrations; B. clausii supernatant (Sup, dilution 1:100) or E. coli K12 (1 × 10 6 cells/mL) as control. Only the exposure to B. clausii strains was able to elicit a significant increase in HBD-2 ( A ) and LL-37 ( B ) production by human enterocytes. HBD 2, human beta defensin 2; LL-37, cathelicidin; NT, untreated. *p < 0.05 vs NT; **p < 0.001 vs NT.

Journal: Scientific Reports

Article Title: Protective action of Bacillus clausii probiotic strains in an in vitro model of Rotavirus infection

doi: 10.1038/s41598-020-69533-7

Figure Lengend Snippet: B. clausii increases HBD-2 and LL-37 expression in human enterocytes. Cells were exposed to B. clausii probiotic strains mix at different concentrations; B. clausii supernatant (Sup, dilution 1:100) or E. coli K12 (1 × 10 6 cells/mL) as control. Only the exposure to B. clausii strains was able to elicit a significant increase in HBD-2 ( A ) and LL-37 ( B ) production by human enterocytes. HBD 2, human beta defensin 2; LL-37, cathelicidin; NT, untreated. *p < 0.05 vs NT; **p < 0.001 vs NT.

Article Snippet: Concentrations of the antimicrobial peptides human beta defensin 2 (HBD-2) and cathelicidin LL-37 in the cell supernatant were determined using commercially available enzyme-linked immunosorbent assay (ELISA) kits specific for human HBD-2 (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) or LL-37 (Hycult biotechnology, Uden, The Netherlands), respectively.

Techniques: Expressing