Journal: Oncology Letters

Article Title: Extracts of Musa basjoo induce growth inhibition and changes in the protein expression of cell cycle control molecules in human colorectal cancer cell lines

doi: 10.3892/ol.2022.13219

Figure Lengend Snippet: Effects of the extracts of Musa basjoo .

Article Snippet: HT29 (HTB38) and HCT116 (CCL247) human colorectal cancer, HepG2 (HB8065) liver cancer/hepatoma, MCF-7 (HTB22) human breast cancer, and PC-3 (CRL1435) human prostate cancer cell lines [American Type Culture Collection (ATCC)] were maintained in Dulbecco's modified Eagle's medium (DMEM) (FUJIFILM Wako Pure Chemical Corp.) supplemented with 5% (v/v) fetal bovine serum (FBS) (Biowest) in an incubator with humidified air at 37°C with 5% CO 2 .

Techniques:

Journal: Oncology Letters

Article Title: Extracts of Musa basjoo induce growth inhibition and changes in the protein expression of cell cycle control molecules in human colorectal cancer cell lines

doi: 10.3892/ol.2022.13219

Figure Lengend Snippet: Effects of the extracts of Musa basjoo .

Article Snippet: HT29 (HTB38) and HCT116 (CCL247) human colorectal cancer, HepG2 (HB8065) liver cancer/hepatoma, MCF-7 (HTB22) human breast cancer, and PC-3 (CRL1435) human prostate cancer cell lines [American Type Culture Collection (ATCC)] were maintained in Dulbecco's modified Eagle's medium (DMEM) (FUJIFILM Wako Pure Chemical Corp.) supplemented with 5% (v/v) fetal bovine serum (FBS) (Biowest) in an incubator with humidified air at 37°C with 5% CO 2 .

Techniques:

Journal: Oncology Letters

Article Title: Extracts of Musa basjoo induce growth inhibition and changes in the protein expression of cell cycle control molecules in human colorectal cancer cell lines

doi: 10.3892/ol.2022.13219

Figure Lengend Snippet: Growth inhibition by the crude extracts of MB in HT29 and HCT116 cell lines in MTT assay. Exponentially dividing cells were treated with acetone/methanol extracts of MB at a dose range of 25-400 µg/ml for 96 h in DMEM/5% FBS. Results are the means ± SEs. The IC 50 values are presented in . MB, Musa basjoo .

Article Snippet: HT29 (HTB38) and HCT116 (CCL247) human colorectal cancer, HepG2 (HB8065) liver cancer/hepatoma, MCF-7 (HTB22) human breast cancer, and PC-3 (CRL1435) human prostate cancer cell lines [American Type Culture Collection (ATCC)] were maintained in Dulbecco's modified Eagle's medium (DMEM) (FUJIFILM Wako Pure Chemical Corp.) supplemented with 5% (v/v) fetal bovine serum (FBS) (Biowest) in an incubator with humidified air at 37°C with 5% CO 2 .

Techniques: Inhibition, MTT Assay

Journal: Oncology Letters

Article Title: Extracts of Musa basjoo induce growth inhibition and changes in the protein expression of cell cycle control molecules in human colorectal cancer cell lines

doi: 10.3892/ol.2022.13219

Figure Lengend Snippet: Representative results of flow cytometry analysis and graphs indicating the quantified results. HT29 and HCT116 cells were treated with DMSO (control) or 100 µg/ml acetone extract of MB for 96 h. The data indicate the percentage of cells in each phase of the cell cycle. Asterisk (*) indicates a statistically significant difference between cells treated with DMSO and those treated with the acetone extract (*P<0.05). Each assay was performed in triplicate. MB, Musa basjoo .

Article Snippet: HT29 (HTB38) and HCT116 (CCL247) human colorectal cancer, HepG2 (HB8065) liver cancer/hepatoma, MCF-7 (HTB22) human breast cancer, and PC-3 (CRL1435) human prostate cancer cell lines [American Type Culture Collection (ATCC)] were maintained in Dulbecco's modified Eagle's medium (DMEM) (FUJIFILM Wako Pure Chemical Corp.) supplemented with 5% (v/v) fetal bovine serum (FBS) (Biowest) in an incubator with humidified air at 37°C with 5% CO 2 .

Techniques: Flow Cytometry

Journal: Oncology Letters

Article Title: Extracts of Musa basjoo induce growth inhibition and changes in the protein expression of cell cycle control molecules in human colorectal cancer cell lines

doi: 10.3892/ol.2022.13219

Figure Lengend Snippet: Representative results of western blot assays (A) and densitometry indicating relative band intensity (B). HT29 and HCT116 cells were treated with 0, 50 or 100 µg/ml acetone extract of MB for 96 h in DMEM/5% FBS. Asterisk (*) indicates a statistically significant difference between cells treated with DMSO and those treated with 100 µg/ml acetone extract of MB (*P<0.05). Each assay was repeated in triplicate to confirm the results. MB, Musa basjoo .

Article Snippet: HT29 (HTB38) and HCT116 (CCL247) human colorectal cancer, HepG2 (HB8065) liver cancer/hepatoma, MCF-7 (HTB22) human breast cancer, and PC-3 (CRL1435) human prostate cancer cell lines [American Type Culture Collection (ATCC)] were maintained in Dulbecco's modified Eagle's medium (DMEM) (FUJIFILM Wako Pure Chemical Corp.) supplemented with 5% (v/v) fetal bovine serum (FBS) (Biowest) in an incubator with humidified air at 37°C with 5% CO 2 .

Techniques: Western Blot