Journal: International Journal of Molecular Sciences
Article Title: The Annexin A1 Receptor FPR2 Regulates the Endosomal Export of Influenza Virus
Figure Lengend Snippet: Cell viability and antiviral activity of the anti-FPR2 mAb ( A ) A549 cells were incubated with 10 or 20 µg/mL of the anti-FPR2 mAb for 24 h, and percentage of cell viability was assessed by trypan blue staining; ( B ) A549 cells were preincubated with 20 µg/mL of anti-FPR2 mAb or 5 µM of the FPR2 antagonist WRW4 and then infected with IAV A/PR/8/34 virus (MOI 1). 24 h after infection, infectious virus titers were determined by plaque assay. The Manne Whitney test was used for statistical analysis and results were considered statistically significant at p
Article Snippet: The following reagents were used in this study: antiviral M2 protein (Santa Cruz, Heidelberg, Germany), Alexa Fluor secondary antibodies (Life Technologies, Villebon-sur-Yvette, France), DAPI (4′,6′-diamidino-2-phenylindole, Sigma-Aldrich, Darmstadt, Germany), phalloidin (Invitrogen, Villebon-sur-Yvette, France), purple crystal staining (Sigma, Darmstadt, Germany), FPR2 antagonists WRW4 (Alomone Labs, Jerusalem, Israel), monoclonal anti-FPR2 antibody (clone FN-1D6-AI, Genova, Italy), monoclonal anti-Nucleoprotein (NP, HB-65, invivomab), conjugated Alexa Fluor 488 anti-Rab5 antibody (Santa Cruz, Heidelberg, Germany), IgG control antibody (ThermoFisher, Villebon-sur-Yvette, France), ERK inhibitor pathway U0126 (Sigma-Aldrich, Darmstadt, Germany).
Techniques: Activity Assay, Incubation, Staining, Infection, Plaque Assay