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  • 90
    Alomone Labs sligkv nh2
    Sligkv Nh2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sligkv nh2/product/Alomone Labs
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    93
    R&D Systems recombinant human sgp130 fc
    IL6 trans-signaling converts non-stem cells into stem-like cells. a MCF 10A spheres cultured without or with IL6, IL6 plus <t>sgp130-Fc</t> or with HIL6. b CFSE-labeled MCF 10A cells were cultured as spheres with or without activators (IL6, HIL6) and inhibitors of classical (an anti-IL6 antibody) and trans-signaling (sgp130-Fc). CFSE-dilution in CD44 high CD24 low , CD44 high CD24 high and CD44 low CD24 high/intermediate cells was determined by flow cytometry at day 4. The CFSE-fluorescence intensity of all cells at day one is included as reference. Data are representative for three 3 independently performed experiments. c The absolute number of CD44 high CD24 low , CD44 high CD24 high , CD44 low CD24 high/intermediate cells (upper panel) and LRCs (CFSE high , lower panel) was determined as cell/bead ratio at day 4 by flow cytometry (N=4-5 per group). d Fold-change correlation analysis comparing gene expression changes induced by IL6 plus sgp130 (classical signaling) and HIL6 (trans signaling) in MCF 10 A cells at 12 and 24 hrs with the gene expression signatures of luminal progenitor (LumProg), mature luminal (MatLum) and mammary stem cell enriched cells (MaSC) according to the study of Lim et al. 35 Nc cor: non-centered correlation between fold-changes, Num: number of common differentially expressed genes. e nLRCs from primary, PKH26-labelled control mammosphere-cultures were sorted by flow cytometry as PKH - cells. f Primary HMECs were cultured as spheres for two consecutive rounds in the absence (N=26) or presence of HIL6 and IL6 (HIL6+HIL6, N=18; IL6+HIL6, N=15; HIL6+IL6, N=14; IL6+IL6, N=17). P values in panel c: one-way ANOVA with Dunett’s multiple comparisons test (post-hoc); panel d: P values according to Student’s t-distribution for Nc cor and hypergeometric testing for Num. panel f: one-way ANOVA with Tukey’s multiple comparisons test (post-hoc); comparisons between groups labeled in red are depicted in the bar graph. Asterisks indicate significance between groups (* P
    Recombinant Human Sgp130 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    IL6 trans-signaling converts non-stem cells into stem-like cells. a MCF 10A spheres cultured without or with IL6, IL6 plus sgp130-Fc or with HIL6. b CFSE-labeled MCF 10A cells were cultured as spheres with or without activators (IL6, HIL6) and inhibitors of classical (an anti-IL6 antibody) and trans-signaling (sgp130-Fc). CFSE-dilution in CD44 high CD24 low , CD44 high CD24 high and CD44 low CD24 high/intermediate cells was determined by flow cytometry at day 4. The CFSE-fluorescence intensity of all cells at day one is included as reference. Data are representative for three 3 independently performed experiments. c The absolute number of CD44 high CD24 low , CD44 high CD24 high , CD44 low CD24 high/intermediate cells (upper panel) and LRCs (CFSE high , lower panel) was determined as cell/bead ratio at day 4 by flow cytometry (N=4-5 per group). d Fold-change correlation analysis comparing gene expression changes induced by IL6 plus sgp130 (classical signaling) and HIL6 (trans signaling) in MCF 10 A cells at 12 and 24 hrs with the gene expression signatures of luminal progenitor (LumProg), mature luminal (MatLum) and mammary stem cell enriched cells (MaSC) according to the study of Lim et al. 35 Nc cor: non-centered correlation between fold-changes, Num: number of common differentially expressed genes. e nLRCs from primary, PKH26-labelled control mammosphere-cultures were sorted by flow cytometry as PKH - cells. f Primary HMECs were cultured as spheres for two consecutive rounds in the absence (N=26) or presence of HIL6 and IL6 (HIL6+HIL6, N=18; IL6+HIL6, N=15; HIL6+IL6, N=14; IL6+IL6, N=17). P values in panel c: one-way ANOVA with Dunett’s multiple comparisons test (post-hoc); panel d: P values according to Student’s t-distribution for Nc cor and hypergeometric testing for Num. panel f: one-way ANOVA with Tukey’s multiple comparisons test (post-hoc); comparisons between groups labeled in red are depicted in the bar graph. Asterisks indicate significance between groups (* P

    Journal: bioRxiv

    Article Title: Interleukin-6 trans-signaling is a candidate mechanism to drive progression of human DCCs during periods of clinical latency

    doi: 10.1101/2020.05.28.121145

    Figure Lengend Snippet: IL6 trans-signaling converts non-stem cells into stem-like cells. a MCF 10A spheres cultured without or with IL6, IL6 plus sgp130-Fc or with HIL6. b CFSE-labeled MCF 10A cells were cultured as spheres with or without activators (IL6, HIL6) and inhibitors of classical (an anti-IL6 antibody) and trans-signaling (sgp130-Fc). CFSE-dilution in CD44 high CD24 low , CD44 high CD24 high and CD44 low CD24 high/intermediate cells was determined by flow cytometry at day 4. The CFSE-fluorescence intensity of all cells at day one is included as reference. Data are representative for three 3 independently performed experiments. c The absolute number of CD44 high CD24 low , CD44 high CD24 high , CD44 low CD24 high/intermediate cells (upper panel) and LRCs (CFSE high , lower panel) was determined as cell/bead ratio at day 4 by flow cytometry (N=4-5 per group). d Fold-change correlation analysis comparing gene expression changes induced by IL6 plus sgp130 (classical signaling) and HIL6 (trans signaling) in MCF 10 A cells at 12 and 24 hrs with the gene expression signatures of luminal progenitor (LumProg), mature luminal (MatLum) and mammary stem cell enriched cells (MaSC) according to the study of Lim et al. 35 Nc cor: non-centered correlation between fold-changes, Num: number of common differentially expressed genes. e nLRCs from primary, PKH26-labelled control mammosphere-cultures were sorted by flow cytometry as PKH - cells. f Primary HMECs were cultured as spheres for two consecutive rounds in the absence (N=26) or presence of HIL6 and IL6 (HIL6+HIL6, N=18; IL6+HIL6, N=15; HIL6+IL6, N=14; IL6+IL6, N=17). P values in panel c: one-way ANOVA with Dunett’s multiple comparisons test (post-hoc); panel d: P values according to Student’s t-distribution for Nc cor and hypergeometric testing for Num. panel f: one-way ANOVA with Tukey’s multiple comparisons test (post-hoc); comparisons between groups labeled in red are depicted in the bar graph. Asterisks indicate significance between groups (* P

    Article Snippet: For some analyses mammosphere media was supplemented additionally with 10 ng/ml IL6 (Sigma-Aldrich, Germany), 1.5 µg/ml anti-IL6 antibody (Sigma-Aldrich, Germany), 20 ng/ml Hyper-IL6, 0.1 or 10 ng/ml recombinant human sgp130-Fc (R & D Systems, Germany).

    Techniques: Cell Culture, Labeling, Flow Cytometry, Fluorescence, Expressing

    IL6 trans-signaling regulates the frequency of MCF 10A, hTERT-HME1 and primary HMECs with sphere-forming ability. a MCF 10A cells were cultured as spheres in the absence (N=18) or presence of IL6 (N=18), an IL6-blocking antibody (N=6) or Hyper-IL6 (N=6). b hTERT-HME1 were cultured as spheres in the absence (N=3) or presence (N=3) of Hyper-IL6. c HMECs were cultured without or with IL6, with an IL6 blocking antibody or Hyper-IL6. N=3 patients, each patient analyzed in triplicate. d hTERT-HME1-EGFR Δ746–750 cells were cultured as spheres in the absence or presence of HIL6 (each N=12). e MCF 10A cells were cultured as spheres without (N=6) or with IL6 (N=6) and IL6 plus sgp130-Fc at indicated concentrations (each N=6). f Sphere formation of hTERT-HME1-EGFR Δ746-750 in the absence (N=10) or presence of an anti-IL6 antibody (N=9) or with sgp130-Fc at indicated concentrations (each N=12). Cumulative data of three experiments. P values in panel a, c, f: one-way ANOVA with Dunnett’s multiple comparisons test (post hoc); panel b, d: two-sided Student’s t-test; panel e: one-way ANOVA with Tukey’s multiple comparisons test (post hoc); asterisks indicate significance between groups (*P

    Journal: bioRxiv

    Article Title: Interleukin-6 trans-signaling is a candidate mechanism to drive progression of human DCCs during periods of clinical latency

    doi: 10.1101/2020.05.28.121145

    Figure Lengend Snippet: IL6 trans-signaling regulates the frequency of MCF 10A, hTERT-HME1 and primary HMECs with sphere-forming ability. a MCF 10A cells were cultured as spheres in the absence (N=18) or presence of IL6 (N=18), an IL6-blocking antibody (N=6) or Hyper-IL6 (N=6). b hTERT-HME1 were cultured as spheres in the absence (N=3) or presence (N=3) of Hyper-IL6. c HMECs were cultured without or with IL6, with an IL6 blocking antibody or Hyper-IL6. N=3 patients, each patient analyzed in triplicate. d hTERT-HME1-EGFR Δ746–750 cells were cultured as spheres in the absence or presence of HIL6 (each N=12). e MCF 10A cells were cultured as spheres without (N=6) or with IL6 (N=6) and IL6 plus sgp130-Fc at indicated concentrations (each N=6). f Sphere formation of hTERT-HME1-EGFR Δ746-750 in the absence (N=10) or presence of an anti-IL6 antibody (N=9) or with sgp130-Fc at indicated concentrations (each N=12). Cumulative data of three experiments. P values in panel a, c, f: one-way ANOVA with Dunnett’s multiple comparisons test (post hoc); panel b, d: two-sided Student’s t-test; panel e: one-way ANOVA with Tukey’s multiple comparisons test (post hoc); asterisks indicate significance between groups (*P

    Article Snippet: For some analyses mammosphere media was supplemented additionally with 10 ng/ml IL6 (Sigma-Aldrich, Germany), 1.5 µg/ml anti-IL6 antibody (Sigma-Aldrich, Germany), 20 ng/ml Hyper-IL6, 0.1 or 10 ng/ml recombinant human sgp130-Fc (R & D Systems, Germany).

    Techniques: Cell Culture, Blocking Assay

    ELISA analysis of soluble OSMR and soluble LIFR binding with LIF, OSM-WT, OSM-M1, and OSM-M2 or gp130-bound LIF (gp130-LIF), OSM-WT (gp130-OSM-WT), OSM-M1 (gp130-OSM-M1), and OSM-M2 (gp130-OSM-M2). Cytokines (LIF, OSM-WT, OSM-M1, or OSM-M2) immobilized

    Journal: The Journal of Biological Chemistry

    Article Title: A Unique Loop Structure in Oncostatin M Determines Binding Affinity toward Oncostatin M Receptor and Leukemia Inhibitory Factor Receptor *

    doi: 10.1074/jbc.M112.387324

    Figure Lengend Snippet: ELISA analysis of soluble OSMR and soluble LIFR binding with LIF, OSM-WT, OSM-M1, and OSM-M2 or gp130-bound LIF (gp130-LIF), OSM-WT (gp130-OSM-WT), OSM-M1 (gp130-OSM-M1), and OSM-M2 (gp130-OSM-M2). Cytokines (LIF, OSM-WT, OSM-M1, or OSM-M2) immobilized

    Article Snippet: For interactions of a higher order, soluble human gp130 (catalog no. 671-GP-100, R & D Systems) was immobilized on 96-well ELISA microplates by incubating the wells with 200 μl of 1 n m gp130 solution (in PBS (pH 7.4)) overnight at 4 °C.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    Kinetic analysis of soluble LIFR and soluble gp130 interaction with LIF, OSM-WT, OSM-M1, or OSM-M2. Soluble LIFR ( left panels ) or soluble gp130 ( right panels ) at various concentrations were injected over an SPR sensor chip with immobilized ligand (LIF,

    Journal: The Journal of Biological Chemistry

    Article Title: A Unique Loop Structure in Oncostatin M Determines Binding Affinity toward Oncostatin M Receptor and Leukemia Inhibitory Factor Receptor *

    doi: 10.1074/jbc.M112.387324

    Figure Lengend Snippet: Kinetic analysis of soluble LIFR and soluble gp130 interaction with LIF, OSM-WT, OSM-M1, or OSM-M2. Soluble LIFR ( left panels ) or soluble gp130 ( right panels ) at various concentrations were injected over an SPR sensor chip with immobilized ligand (LIF,

    Article Snippet: For interactions of a higher order, soluble human gp130 (catalog no. 671-GP-100, R & D Systems) was immobilized on 96-well ELISA microplates by incubating the wells with 200 μl of 1 n m gp130 solution (in PBS (pH 7.4)) overnight at 4 °C.

    Techniques: Injection, SPR Assay, Chromatin Immunoprecipitation