GDH Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Proteintech glutamate transport system permease protein
    Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in nonadherent MIAPaCa-2 (mutant KRAS) and BxPC3 (wild-type KRAS) cancer cells treated with CPT. A total of 5×10 5 cells were seeded into poly-HEMA-coated 60 mm dishes and treated with CPT at 10 and 20 µ M for 48 h. GAPDH was used as the internal control. KRAS, Kirsten rat sarcoma viral oncogene homolog; CPT, cryptotanshinone; GOT, glutamic-oxaloacetic transaminase; GLUD, <t>glutamate</t> <t>transport</t> <t>system</t> <t>permease</t> <t>protein;</t> GLS, glutaminase; IDH, isocitrate dehydrogenase; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; p-, phosphorylated.
    Glutamate Transport System Permease Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutamate transport system permease protein/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glutamate transport system permease protein - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    85
    Rockland Immunochemicals gdh antibody
    Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in nonadherent MIAPaCa-2 (mutant KRAS) and BxPC3 (wild-type KRAS) cancer cells treated with CPT. A total of 5×10 5 cells were seeded into poly-HEMA-coated 60 mm dishes and treated with CPT at 10 and 20 µ M for 48 h. GAPDH was used as the internal control. KRAS, Kirsten rat sarcoma viral oncogene homolog; CPT, cryptotanshinone; GOT, glutamic-oxaloacetic transaminase; GLUD, <t>glutamate</t> <t>transport</t> <t>system</t> <t>permease</t> <t>protein;</t> GLS, glutaminase; IDH, isocitrate dehydrogenase; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; p-, phosphorylated.
    Gdh Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdh antibody/product/Rockland Immunochemicals
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdh antibody - by Bioz Stars, 2024-07
    85/100 stars
      Buy from Supplier

    93
    Proteintech glud1
    Resibufogenin induces RIP3-dependent necroptosis through activating PYGL, GLUL and <t>GLUD1.</t> a Immunoblot analysis of necrotic proteins were determined after 24 h of resibufogenin treatment. Level of Bax, P53, and PGAM5 in cytosol and mitochondrial fractions from HCT116 cells treated with resibufogenin at the indicated concentrations for 24 h were evaluated by western blot analysis. β-Actin was used as a loading control. See also Additional file and Additional file : Figures S7, S8. The gray value of each stripe has been calculated using quantity one software. b Confocal immunofluorescence of RIP3 (red, anti-RIP3) in the HCT116 and SW620 cells that were loaded with MitoTracker and DAPI (n = 3). ×1000 for all, scale bar = 1 μm. c RIP3 + / + and RIP3 − / − cells were treated with resibufogenin for 24 h. cells viability was measured by MTT assay (n = 6). See also Additional file and Additional file : Figure S9. d The activity of three energy metabolism enzymes in the two cell lines (n = 3). e The protein expression of three energy—metabolizing enzymes in two cell lines. Data represent mean ± SEM, # P < 0.05, * P < 0.01, as determined by one-way ANOVA followed by Tukey’s multiple comparison test
    Glud1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glud1/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glud1 - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    90
    Proteintech ugdh
    Identified differentially accumulated proteins related to glucose metabolism.
    Ugdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ugdh/product/Proteintech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ugdh - by Bioz Stars, 2024-07
    90/100 stars
      Buy from Supplier

    93
    Proteintech anti gdh
    Identified differentially accumulated proteins related to glucose metabolism.
    Anti Gdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gdh/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gdh - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in nonadherent MIAPaCa-2 (mutant KRAS) and BxPC3 (wild-type KRAS) cancer cells treated with CPT. A total of 5×10 5 cells were seeded into poly-HEMA-coated 60 mm dishes and treated with CPT at 10 and 20 µ M for 48 h. GAPDH was used as the internal control. KRAS, Kirsten rat sarcoma viral oncogene homolog; CPT, cryptotanshinone; GOT, glutamic-oxaloacetic transaminase; GLUD, glutamate transport system permease protein; GLS, glutaminase; IDH, isocitrate dehydrogenase; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; p-, phosphorylated.

    Journal: International Journal of Oncology

    Article Title: Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS-activated pancreatic cancer cells

    doi: 10.3892/ijo.2022.5398

    Figure Lengend Snippet: Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in nonadherent MIAPaCa-2 (mutant KRAS) and BxPC3 (wild-type KRAS) cancer cells treated with CPT. A total of 5×10 5 cells were seeded into poly-HEMA-coated 60 mm dishes and treated with CPT at 10 and 20 µ M for 48 h. GAPDH was used as the internal control. KRAS, Kirsten rat sarcoma viral oncogene homolog; CPT, cryptotanshinone; GOT, glutamic-oxaloacetic transaminase; GLUD, glutamate transport system permease protein; GLS, glutaminase; IDH, isocitrate dehydrogenase; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; p-, phosphorylated.

    Article Snippet: Rabbit monoclonal antibodies for GOT1 (1:3,000; cat. no. 14886-1-AP), isocitrate dehydrogenase (IDH)2 (15932-1-AP) and glutamate transport system permease protein (GLUD)1 (1:3,000; cat. no. 14299-1AP) and mouse monoclonal antibodies for GLS (1:3,000; cat. no. 66265-1-Ig) were purchased from ProteinTech Group, Inc.

    Techniques: Western Blot, Mutagenesis

    Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in MIAPaCa-2 cells treated with control (siC) and KRAS siRNA (siKRAS). (A) A total of 1×10 5 cells were treated with siRNA for 24 h, followed by nonadherent culture (48 h). GAPDH was used as the internal control. (B) Quantitative analysis of protein expression levels of K-Ras, FASN and ACC1 after siKRAS treatment in MIAPaCa-2 cells. The bars indicate the relative expression value normalized to those of GAPDH and are presented as mean ± standard deviation of three independent assays. * P<0.05 compared with siC using Welch's t-test. KRAS, Kirsten rat sarcoma viral oncogene homolog; si, short interfering; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; GOT, glutamic-oxaloacetic transaminase; IDH, isocitrate dehydrogenase; GLUD, glutamate transport system permease protein; GLS, glutaminase; p-, phosphorylated.

    Journal: International Journal of Oncology

    Article Title: Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS-activated pancreatic cancer cells

    doi: 10.3892/ijo.2022.5398

    Figure Lengend Snippet: Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in MIAPaCa-2 cells treated with control (siC) and KRAS siRNA (siKRAS). (A) A total of 1×10 5 cells were treated with siRNA for 24 h, followed by nonadherent culture (48 h). GAPDH was used as the internal control. (B) Quantitative analysis of protein expression levels of K-Ras, FASN and ACC1 after siKRAS treatment in MIAPaCa-2 cells. The bars indicate the relative expression value normalized to those of GAPDH and are presented as mean ± standard deviation of three independent assays. * P<0.05 compared with siC using Welch's t-test. KRAS, Kirsten rat sarcoma viral oncogene homolog; si, short interfering; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; GOT, glutamic-oxaloacetic transaminase; IDH, isocitrate dehydrogenase; GLUD, glutamate transport system permease protein; GLS, glutaminase; p-, phosphorylated.

    Article Snippet: Rabbit monoclonal antibodies for GOT1 (1:3,000; cat. no. 14886-1-AP), isocitrate dehydrogenase (IDH)2 (15932-1-AP) and glutamate transport system permease protein (GLUD)1 (1:3,000; cat. no. 14299-1AP) and mouse monoclonal antibodies for GLS (1:3,000; cat. no. 66265-1-Ig) were purchased from ProteinTech Group, Inc.

    Techniques: Western Blot, Expressing, Standard Deviation

    Schematic illustration of the antioncogenic effect of CPT mediated by mutant K-Ras protein. KRAS, Kirsten rat sarcoma viral oncogene homolog; CPT, cryptotanshinone; GOT, glutamic-oxaloacetic transaminase; GLUD, glutamate transport system permease protein; GLS, glutaminase; IDH, isocitrate dehydrogenase; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; ROS, oxygen species.

    Journal: International Journal of Oncology

    Article Title: Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS-activated pancreatic cancer cells

    doi: 10.3892/ijo.2022.5398

    Figure Lengend Snippet: Schematic illustration of the antioncogenic effect of CPT mediated by mutant K-Ras protein. KRAS, Kirsten rat sarcoma viral oncogene homolog; CPT, cryptotanshinone; GOT, glutamic-oxaloacetic transaminase; GLUD, glutamate transport system permease protein; GLS, glutaminase; IDH, isocitrate dehydrogenase; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; ROS, oxygen species.

    Article Snippet: Rabbit monoclonal antibodies for GOT1 (1:3,000; cat. no. 14886-1-AP), isocitrate dehydrogenase (IDH)2 (15932-1-AP) and glutamate transport system permease protein (GLUD)1 (1:3,000; cat. no. 14299-1AP) and mouse monoclonal antibodies for GLS (1:3,000; cat. no. 66265-1-Ig) were purchased from ProteinTech Group, Inc.

    Techniques: Mutagenesis

    Resibufogenin induces RIP3-dependent necroptosis through activating PYGL, GLUL and GLUD1. a Immunoblot analysis of necrotic proteins were determined after 24 h of resibufogenin treatment. Level of Bax, P53, and PGAM5 in cytosol and mitochondrial fractions from HCT116 cells treated with resibufogenin at the indicated concentrations for 24 h were evaluated by western blot analysis. β-Actin was used as a loading control. See also Additional file and Additional file : Figures S7, S8. The gray value of each stripe has been calculated using quantity one software. b Confocal immunofluorescence of RIP3 (red, anti-RIP3) in the HCT116 and SW620 cells that were loaded with MitoTracker and DAPI (n = 3). ×1000 for all, scale bar = 1 μm. c RIP3 + / + and RIP3 − / − cells were treated with resibufogenin for 24 h. cells viability was measured by MTT assay (n = 6). See also Additional file and Additional file : Figure S9. d The activity of three energy metabolism enzymes in the two cell lines (n = 3). e The protein expression of three energy—metabolizing enzymes in two cell lines. Data represent mean ± SEM, # P < 0.05, * P < 0.01, as determined by one-way ANOVA followed by Tukey’s multiple comparison test

    Journal: Journal of Translational Medicine

    Article Title: Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis

    doi: 10.1186/s12967-018-1580-x

    Figure Lengend Snippet: Resibufogenin induces RIP3-dependent necroptosis through activating PYGL, GLUL and GLUD1. a Immunoblot analysis of necrotic proteins were determined after 24 h of resibufogenin treatment. Level of Bax, P53, and PGAM5 in cytosol and mitochondrial fractions from HCT116 cells treated with resibufogenin at the indicated concentrations for 24 h were evaluated by western blot analysis. β-Actin was used as a loading control. See also Additional file and Additional file : Figures S7, S8. The gray value of each stripe has been calculated using quantity one software. b Confocal immunofluorescence of RIP3 (red, anti-RIP3) in the HCT116 and SW620 cells that were loaded with MitoTracker and DAPI (n = 3). ×1000 for all, scale bar = 1 μm. c RIP3 + / + and RIP3 − / − cells were treated with resibufogenin for 24 h. cells viability was measured by MTT assay (n = 6). See also Additional file and Additional file : Figure S9. d The activity of three energy metabolism enzymes in the two cell lines (n = 3). e The protein expression of three energy—metabolizing enzymes in two cell lines. Data represent mean ± SEM, # P < 0.05, * P < 0.01, as determined by one-way ANOVA followed by Tukey’s multiple comparison test

    Article Snippet: Protein expression was assessed by immunoblot analysis of cell lysates (30–60 μg) in RIPA buffer in the presence of rabbit antibodies to RIP3 (1:1000; Cell Signaling Technology), E-cadherin, fibronectin (FN), β-actin (1:500; Santa Cruz Biotech, CA, USA), GLUD1, PYGL, GLUL (1:500; Proteintech, Danvers, MA, USA), Cyt-c, and apoptosis-inducing factor (AIF) (1:500; Abcam, Cambridge, UK).

    Techniques: Western Blot, Software, Immunofluorescence, MTT Assay, Activity Assay, Expressing

    The expression of necroptosis protein in resibufogenin-, and parthenolide-treated mice. a RIP3, PYGL, GLUD1 and GLUL in tumor tissue lysates from vehicle-, resibufogenin-, and parthenolide-treated mice were detected by western blot analysis (n = 3). b Tumors were excised and processed for immunostaining with RIP3 (green, anti-RIP3), PYGL (green, anti-PYGL), GLUD1 (green, anti-GLUD1), GLUL (green, anti-GLUL) and DAPI (blue), and fluorescent images were obtained by Confocal, ×400 for all, scale bar = 100 μm. See also Additional file and Additional file : Figure S10. c Immunohistochemical staining of RIP3, PYGL, GLUD1 and GLUL expression in tumor tissues. ×200 for all, scale bar = 100 μm

    Journal: Journal of Translational Medicine

    Article Title: Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis

    doi: 10.1186/s12967-018-1580-x

    Figure Lengend Snippet: The expression of necroptosis protein in resibufogenin-, and parthenolide-treated mice. a RIP3, PYGL, GLUD1 and GLUL in tumor tissue lysates from vehicle-, resibufogenin-, and parthenolide-treated mice were detected by western blot analysis (n = 3). b Tumors were excised and processed for immunostaining with RIP3 (green, anti-RIP3), PYGL (green, anti-PYGL), GLUD1 (green, anti-GLUD1), GLUL (green, anti-GLUL) and DAPI (blue), and fluorescent images were obtained by Confocal, ×400 for all, scale bar = 100 μm. See also Additional file and Additional file : Figure S10. c Immunohistochemical staining of RIP3, PYGL, GLUD1 and GLUL expression in tumor tissues. ×200 for all, scale bar = 100 μm

    Article Snippet: Protein expression was assessed by immunoblot analysis of cell lysates (30–60 μg) in RIPA buffer in the presence of rabbit antibodies to RIP3 (1:1000; Cell Signaling Technology), E-cadherin, fibronectin (FN), β-actin (1:500; Santa Cruz Biotech, CA, USA), GLUD1, PYGL, GLUL (1:500; Proteintech, Danvers, MA, USA), Cyt-c, and apoptosis-inducing factor (AIF) (1:500; Abcam, Cambridge, UK).

    Techniques: Expressing, Western Blot, Immunostaining, Immunohistochemical staining, Staining

    Identified differentially accumulated proteins related to glucose metabolism.

    Journal: PLoS ONE

    Article Title: Quantitative Proteomic Analysis Reveals That Anti-Cancer Effects of Selenium-Binding Protein 1 In Vivo Are Associated with Metabolic Pathways

    doi: 10.1371/journal.pone.0126285

    Figure Lengend Snippet: Identified differentially accumulated proteins related to glucose metabolism.

    Article Snippet: Primary antibodies and dilutions including SBP1 at 1:1000 (mouse, MBL International, Woburn, MA), β-actin at 1:10000 (mouse, Sigma), DKK1 at 1:1000 (rabbit, Proteintech, Chicago, IL), ANXA4 at 1:1000 (rabbit, Proteintech, Chicago, IL), PPIA at 1:1000 (rabbit, Proteintech, Chicago, IL), FABP4 at 1:1000 (rabbit, Proteintech, Chicago, IL), UGDH at 1:1000 (rabbit, Proteintech, Chicago, IL), ALDH2 at 1:1000 (rabbit, Proteintech, Chicago, IL), p21 at 1:1000 (rabbit, Proteintech, Chicago, IL), phospho-Akt (Ser473) at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), Akt at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), phospho-FOXO1 at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), phospho-JNK at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), JNK at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), P-c-Jun at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), phospho-Cyclin D1 at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), Cyclin D1 at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), phospho-p53 (Ser15) at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), TWIST at 1:1000 (rabbit, Cell Signaling Technology, Danvers, MA), β–Catenin at 1:500 (goat, Santa Cruz, Santa Cruz, CA), and GPX1 at 1:1000 (mouse, MBL International, Woburn, MA) were incubated with membrane at 4°C overnight.

    Techniques: Activity Assay