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  • 92
    Alomone Labs achr inhibitor fasciculin ii
    Analysis of basal lamina and NMJ functionality in Pax7+/+ and Pax7−/− teratomas. a – c Expression of mRNAs encoding Lama4 , Lama5 , and Lamb2 (for each genotype n = 8 to 6). d , e Expression of mRNAs encoding neuronal markers RbFox3 and Otx2 (for each genotype n = 6 to n = 7). f Immunolocalization of neurofilament (green, left column, TL transmitted light), synaptophysin (red, middle column; BTX—green; nuclei—blue), and <t>Fasciculin</t> (red, right column; BTX—green; nuclei—blue) in teratoma muscles. Scale bar 10 or 100 μm, as indicated. White bars—values for teratomas obtained from Pax7+/+ ESCs, gray bars—values for teratomas obtained from Pax7−/− ESCs. Data are presented as mean ± SD. Stars symbolize results of Student’s unpaired two-tailed t test: * p < 0.05; ** p < 0.01
    Achr Inhibitor Fasciculin Ii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    JASCO Inc f650
    Purification and subsequent analysis of <t>F650/670</t> by HPLC. ( A ) F650/670 sample was injected onto a Delta Pak C18 column, 7.8 × 300 mm 2 , pre-equilibrated in 20 mM phosphate buffer, pH 7.0, flow rate 5 ml/min. The mobile phase was manually changed in the following order: 100% water at 11 min → 20% MeOH/80% water at 18 min → 30% MeOH/70% water at 23 min → 40% MeOH/60% water at 29 min → 50% MeOH/50% water at 35 min → 60% MeOH/40% water at 43 min → 100% MeOH at 52 min. The 41–45 min fraction (marked as F650/670 ) has the desired UV spectrum from an online photodiode array detector. ( B ) Analysis of the 41–45 min fraction by an Econosphere C18 column, 4.6 × 50 mm 2 , in 60% MeOH/40% 20 mM phosphate, pH 7.0, flow rate 0.5 ml/min. The insert shows the UV–visible spectrum of the peak.
    F650, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f650/product/JASCO Inc
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    86
    Beckman Coulter f650 rotor
    Purification and subsequent analysis of <t>F650/670</t> by HPLC. ( A ) F650/670 sample was injected onto a Delta Pak C18 column, 7.8 × 300 mm 2 , pre-equilibrated in 20 mM phosphate buffer, pH 7.0, flow rate 5 ml/min. The mobile phase was manually changed in the following order: 100% water at 11 min → 20% MeOH/80% water at 18 min → 30% MeOH/70% water at 23 min → 40% MeOH/60% water at 29 min → 50% MeOH/50% water at 35 min → 60% MeOH/40% water at 43 min → 100% MeOH at 52 min. The 41–45 min fraction (marked as F650/670 ) has the desired UV spectrum from an online photodiode array detector. ( B ) Analysis of the 41–45 min fraction by an Econosphere C18 column, 4.6 × 50 mm 2 , in 60% MeOH/40% 20 mM phosphate, pH 7.0, flow rate 0.5 ml/min. The insert shows the UV–visible spectrum of the peak.
    F650 Rotor, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f650 rotor/product/Beckman Coulter
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    86
    SAS institute ratio f650 f680
    Purification and subsequent analysis of <t>F650/670</t> by HPLC. ( A ) F650/670 sample was injected onto a Delta Pak C18 column, 7.8 × 300 mm 2 , pre-equilibrated in 20 mM phosphate buffer, pH 7.0, flow rate 5 ml/min. The mobile phase was manually changed in the following order: 100% water at 11 min → 20% MeOH/80% water at 18 min → 30% MeOH/70% water at 23 min → 40% MeOH/60% water at 29 min → 50% MeOH/50% water at 35 min → 60% MeOH/40% water at 43 min → 100% MeOH at 52 min. The 41–45 min fraction (marked as F650/670 ) has the desired UV spectrum from an online photodiode array detector. ( B ) Analysis of the 41–45 min fraction by an Econosphere C18 column, 4.6 × 50 mm 2 , in 60% MeOH/40% 20 mM phosphate, pH 7.0, flow rate 0.5 ml/min. The insert shows the UV–visible spectrum of the peak.
    Ratio F650 F680, supplied by SAS institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of basal lamina and NMJ functionality in Pax7+/+ and Pax7−/− teratomas. a – c Expression of mRNAs encoding Lama4 , Lama5 , and Lamb2 (for each genotype n = 8 to 6). d , e Expression of mRNAs encoding neuronal markers RbFox3 and Otx2 (for each genotype n = 6 to n = 7). f Immunolocalization of neurofilament (green, left column, TL transmitted light), synaptophysin (red, middle column; BTX—green; nuclei—blue), and Fasciculin (red, right column; BTX—green; nuclei—blue) in teratoma muscles. Scale bar 10 or 100 μm, as indicated. White bars—values for teratomas obtained from Pax7+/+ ESCs, gray bars—values for teratomas obtained from Pax7−/− ESCs. Data are presented as mean ± SD. Stars symbolize results of Student’s unpaired two-tailed t test: * p < 0.05; ** p < 0.01

    Journal: Stem Cell Research & Therapy

    Article Title: Pax7 as molecular switch regulating early and advanced stages of myogenic mouse ESC differentiation in teratomas

    doi: 10.1186/s13287-020-01742-3

    Figure Lengend Snippet: Analysis of basal lamina and NMJ functionality in Pax7+/+ and Pax7−/− teratomas. a – c Expression of mRNAs encoding Lama4 , Lama5 , and Lamb2 (for each genotype n = 8 to 6). d , e Expression of mRNAs encoding neuronal markers RbFox3 and Otx2 (for each genotype n = 6 to n = 7). f Immunolocalization of neurofilament (green, left column, TL transmitted light), synaptophysin (red, middle column; BTX—green; nuclei—blue), and Fasciculin (red, right column; BTX—green; nuclei—blue) in teratoma muscles. Scale bar 10 or 100 μm, as indicated. White bars—values for teratomas obtained from Pax7+/+ ESCs, gray bars—values for teratomas obtained from Pax7−/− ESCs. Data are presented as mean ± SD. Stars symbolize results of Student’s unpaired two-tailed t test: * p < 0.05; ** p < 0.01

    Article Snippet: The nonspecific antibody binding sites were blocked by incubation in 3% bovine serum albumin (BSA) in PBS at room temperature for 30 min. Next, specimens were incubated in primary antibody solutions, i.e., antibodies anti: Myf5 (ab125301, Abcam; 1:100), Laminin (L9393, Sigma-Aldrich; 1:500), Laminin (L8271, Sigma-Aldrich; 1:500), skeletal muscle myosin heavy chains (skMyh, M7523, Sigma-Aldrich; 1:100), embryonic isoforms of myosin heavy chains (Myh3, F1.652, DSHB; 1:10), slow isoforms of myosin heavy chains (Myh7, BA-D5, DSHB; 1:10), fast isoforms of myosin heavy chains IIa (Myh2a, 2F7, DSHB; 1:10), fast isoforms of myosin heavy chains IIb (Myh2b, BF-F3, DSHB, 1:50), neurofilament (NF-M, 2H3, DSHB, 1:50), synaptophysin 1 (101,004, Synaptic Systems, 1:50), and AChR inhibitor fasciculin-II (F-650, Alomone Lab, Rhodamine conjugated, 7 μg/ml) diluted in 0.5% BSA in PBS, at 4 °C overnight.

    Techniques: Expressing, Two Tailed Test

    Purification and subsequent analysis of F650/670 by HPLC. ( A ) F650/670 sample was injected onto a Delta Pak C18 column, 7.8 × 300 mm 2 , pre-equilibrated in 20 mM phosphate buffer, pH 7.0, flow rate 5 ml/min. The mobile phase was manually changed in the following order: 100% water at 11 min → 20% MeOH/80% water at 18 min → 30% MeOH/70% water at 23 min → 40% MeOH/60% water at 29 min → 50% MeOH/50% water at 35 min → 60% MeOH/40% water at 43 min → 100% MeOH at 52 min. The 41–45 min fraction (marked as F650/670 ) has the desired UV spectrum from an online photodiode array detector. ( B ) Analysis of the 41–45 min fraction by an Econosphere C18 column, 4.6 × 50 mm 2 , in 60% MeOH/40% 20 mM phosphate, pH 7.0, flow rate 0.5 ml/min. The insert shows the UV–visible spectrum of the peak.

    Journal: Nucleic Acids Research

    Article Title: Novel cyanine-AMP conjugates for efficient 5′ RNA fluorescent labeling by one-step transcription and replacement of [γ- 32 P]ATP in RNA structural investigation

    doi: 10.1093/nar/gni036

    Figure Lengend Snippet: Purification and subsequent analysis of F650/670 by HPLC. ( A ) F650/670 sample was injected onto a Delta Pak C18 column, 7.8 × 300 mm 2 , pre-equilibrated in 20 mM phosphate buffer, pH 7.0, flow rate 5 ml/min. The mobile phase was manually changed in the following order: 100% water at 11 min → 20% MeOH/80% water at 18 min → 30% MeOH/70% water at 23 min → 40% MeOH/60% water at 29 min → 50% MeOH/50% water at 35 min → 60% MeOH/40% water at 43 min → 100% MeOH at 52 min. The 41–45 min fraction (marked as F650/670 ) has the desired UV spectrum from an online photodiode array detector. ( B ) Analysis of the 41–45 min fraction by an Econosphere C18 column, 4.6 × 50 mm 2 , in 60% MeOH/40% 20 mM phosphate, pH 7.0, flow rate 0.5 ml/min. The insert shows the UV–visible spectrum of the peak.

    Article Snippet: UV absorbance spectra and molar extinction coefficiencies of F550/570 and F650/670 were obtained from a JASCO spectrometer (V-530) in 20 mM phosphate, pH 7.0.

    Techniques: Purification, Injection

    RNA fluorescent labeling by F550/570 and F650/670 under the T7 φ2.5 promoter ( , ). All RNA was also internally 32 P-labeled by [α- 32 P]ATP. After transcription, RNA samples were fractionated by PAGE. Lane 1, normal transcription; lanes 2 and 3, transcription in the presence of F550/570 and F650/670 , respectively. ( A ) 32 P-phosphorimaging reveals total RNA bands in different transcription experiments. ( B ) Scanning of the same gel under the excitation of a 532 nm laser shows only F550/570 -labeled RNA. ( C ) Under excitation with a 633 nm laser, only F650/670 -labeled RNA is visible. The RNA sequence was that of a thioester-synthesizing ribozyme TES33, 92 nt . RNAs from ∼5 μl transcription were used for the gel.

    Journal: Nucleic Acids Research

    Article Title: Novel cyanine-AMP conjugates for efficient 5′ RNA fluorescent labeling by one-step transcription and replacement of [γ- 32 P]ATP in RNA structural investigation

    doi: 10.1093/nar/gni036

    Figure Lengend Snippet: RNA fluorescent labeling by F550/570 and F650/670 under the T7 φ2.5 promoter ( , ). All RNA was also internally 32 P-labeled by [α- 32 P]ATP. After transcription, RNA samples were fractionated by PAGE. Lane 1, normal transcription; lanes 2 and 3, transcription in the presence of F550/570 and F650/670 , respectively. ( A ) 32 P-phosphorimaging reveals total RNA bands in different transcription experiments. ( B ) Scanning of the same gel under the excitation of a 532 nm laser shows only F550/570 -labeled RNA. ( C ) Under excitation with a 633 nm laser, only F650/670 -labeled RNA is visible. The RNA sequence was that of a thioester-synthesizing ribozyme TES33, 92 nt . RNAs from ∼5 μl transcription were used for the gel.

    Article Snippet: UV absorbance spectra and molar extinction coefficiencies of F550/570 and F650/670 were obtained from a JASCO spectrometer (V-530) in 20 mM phosphate, pH 7.0.

    Techniques: Labeling, Sequencing

    UV–visible absorption spectra (Ab) and fluorescence emission spectra (Em) of F550/570 and F650/670 . The spectra were measured in 20 mM phosphate buffer, pH 7.0. All spectra were normalized to 1 at their λ max . The λ max difference between excitation and emission is 20 nm for both F550/570 and F650/670 .

    Journal: Nucleic Acids Research

    Article Title: Novel cyanine-AMP conjugates for efficient 5′ RNA fluorescent labeling by one-step transcription and replacement of [γ- 32 P]ATP in RNA structural investigation

    doi: 10.1093/nar/gni036

    Figure Lengend Snippet: UV–visible absorption spectra (Ab) and fluorescence emission spectra (Em) of F550/570 and F650/670 . The spectra were measured in 20 mM phosphate buffer, pH 7.0. All spectra were normalized to 1 at their λ max . The λ max difference between excitation and emission is 20 nm for both F550/570 and F650/670 .

    Article Snippet: UV absorbance spectra and molar extinction coefficiencies of F550/570 and F650/670 were obtained from a JASCO spectrometer (V-530) in 20 mM phosphate, pH 7.0.

    Techniques: Fluorescence