ELISA Antibodies Search Results


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  • 86
    SouthernBiotech igg2b
    <t>IgG1/IgG3</t> ratios for different vaccine antigens. ( a ) IgG1 to <t>IgG3</t> ratios were calculated as log(IgG1/IgG3) for titers measured at wk14 of age. The calculated Pearson correlation coefficients of IgG1/IgG3 ratio (average value of each strain) between the four DTaP antigens were all significant as indicated by the p -values. ( b ) Number of genes identified by genome-wide association mapping algorithm for IgG1/IgG3 ratio were plotted by Venn diagram.
    Igg2b, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2b/product/SouthernBiotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg2b - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher igg2
    The anti-FIS IgM and <t>IgG</t> subclasses <t>(IgG1</t> and <t>IgG2)</t> ELISA titres of cattle are vaccinated at week 0 and 3 with either PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and Emulsigen-D ® /Alhydrogel ® adjuvants (NegCtl) (n = 4) are presented as box and whisker plots. The cattle sera samples were collected before the vaccinations at week 0 and 3 as well as at week 5 and 12. Sera dilution started at a concentration of 1:50 and values below the (
    Igg2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg2 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    pbs  (Abcam)
    99
    Abcam pbs
    The anti-FIS IgM and <t>IgG</t> subclasses <t>(IgG1</t> and <t>IgG2)</t> ELISA titres of cattle are vaccinated at week 0 and 3 with either PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and Emulsigen-D ® /Alhydrogel ® adjuvants (NegCtl) (n = 4) are presented as box and whisker plots. The cattle sera samples were collected before the vaccinations at week 0 and 3 as well as at week 5 and 12. Sera dilution started at a concentration of 1:50 and values below the (
    Pbs, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    99
    Millipore monoclonal anti flag m2 antibody
    The anti-FIS IgM and <t>IgG</t> subclasses <t>(IgG1</t> and <t>IgG2)</t> ELISA titres of cattle are vaccinated at week 0 and 3 with either PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and Emulsigen-D ® /Alhydrogel ® adjuvants (NegCtl) (n = 4) are presented as box and whisker plots. The cattle sera samples were collected before the vaccinations at week 0 and 3 as well as at week 5 and 12. Sera dilution started at a concentration of 1:50 and values below the (
    Monoclonal Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti flag m2 antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti flag m2 antibody - by Bioz Stars, 2021-05
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    N/A
    The Anti Transglutaminase Antibodies ELISA is intended for the quantitative measurement of anti transglutaminase antibodies in stool samples This ELISA is one of twelve that utilizes a single stool extraction
      Buy from Supplier

    N/A
    The Anti Gliadin Antibodies ELISA is intended for the quantitative determination of anti gliadin antibodies in human stool samples Compatible with the Easy Stool Extraction Device IgA subclass minimizes false
      Buy from Supplier

    N/A
    Human Anti Mouse Antibodies ELISA HAMA ELISA
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    N/A
    ELISA kit for the detection of Sperm Antibodies in the research laboratory
      Buy from Supplier

    Image Search Results


    IgG1/IgG3 ratios for different vaccine antigens. ( a ) IgG1 to IgG3 ratios were calculated as log(IgG1/IgG3) for titers measured at wk14 of age. The calculated Pearson correlation coefficients of IgG1/IgG3 ratio (average value of each strain) between the four DTaP antigens were all significant as indicated by the p -values. ( b ) Number of genes identified by genome-wide association mapping algorithm for IgG1/IgG3 ratio were plotted by Venn diagram.

    Journal: Vaccines

    Article Title: Genetic Variation in the Magnitude and Longevity of the IgG Subclass Response to a Diphtheria-Tetanus-Acellular Pertussis (DTaP) Vaccine in Mice

    doi: 10.3390/vaccines7040124

    Figure Lengend Snippet: IgG1/IgG3 ratios for different vaccine antigens. ( a ) IgG1 to IgG3 ratios were calculated as log(IgG1/IgG3) for titers measured at wk14 of age. The calculated Pearson correlation coefficients of IgG1/IgG3 ratio (average value of each strain) between the four DTaP antigens were all significant as indicated by the p -values. ( b ) Number of genes identified by genome-wide association mapping algorithm for IgG1/IgG3 ratio were plotted by Venn diagram.

    Article Snippet: The following are available online at https://www.mdpi.com/2076-393X/7/4/124/s1 , Figure S1: Longevity of IgG1, IgG2b and IgG3 to DTaP vaccine antigens in inbred mouse strains, Figure S2: IgG1, IgG2b and IgG3 titers at 24 weeks of age, 12 weeks after the last vaccination, Figure S3: Correlations between the magnitude of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S4: Correlations between the longevity of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S5: Correlations between the magnitude of IgG1 (a), IgG2b (b) and IgG3 (c) titers against different vaccine antigens, Figure S6: Correlations between the longevity of IgG1, IgG2b and IgG3 titers against different vaccine antigens, Figure S7: Venn diagrams for common genes associated with antibody titers and longevity, Figure S8: IgG1/IgG3 ratio against different vaccine antigens in BALB/cJ and C57BL/6J mice, Figure S9: Effect of TLR4-deficiency on the IgG1/IgG2a and IgG1/IgG3 ratios in mice immunized with DTaP.

    Techniques: GWAS

    Correlations between antigen-specific responses among IgG1, IgG2b, and IgG3. The correlations between antibody response to different vaccine antigens for IgG1, IgG2b, and IgG3 were plotted for antibody magnitude ( a ) and longevity ( b ). The correlations were presented by Pearson r values for each pair of antigen-specific antibody within the same IgG subclass. Significant values ( p

    Journal: Vaccines

    Article Title: Genetic Variation in the Magnitude and Longevity of the IgG Subclass Response to a Diphtheria-Tetanus-Acellular Pertussis (DTaP) Vaccine in Mice

    doi: 10.3390/vaccines7040124

    Figure Lengend Snippet: Correlations between antigen-specific responses among IgG1, IgG2b, and IgG3. The correlations between antibody response to different vaccine antigens for IgG1, IgG2b, and IgG3 were plotted for antibody magnitude ( a ) and longevity ( b ). The correlations were presented by Pearson r values for each pair of antigen-specific antibody within the same IgG subclass. Significant values ( p

    Article Snippet: The following are available online at https://www.mdpi.com/2076-393X/7/4/124/s1 , Figure S1: Longevity of IgG1, IgG2b and IgG3 to DTaP vaccine antigens in inbred mouse strains, Figure S2: IgG1, IgG2b and IgG3 titers at 24 weeks of age, 12 weeks after the last vaccination, Figure S3: Correlations between the magnitude of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S4: Correlations between the longevity of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S5: Correlations between the magnitude of IgG1 (a), IgG2b (b) and IgG3 (c) titers against different vaccine antigens, Figure S6: Correlations between the longevity of IgG1, IgG2b and IgG3 titers against different vaccine antigens, Figure S7: Venn diagrams for common genes associated with antibody titers and longevity, Figure S8: IgG1/IgG3 ratio against different vaccine antigens in BALB/cJ and C57BL/6J mice, Figure S9: Effect of TLR4-deficiency on the IgG1/IgG2a and IgG1/IgG3 ratios in mice immunized with DTaP.

    Techniques:

    Effect of TLR4 on the magnitude antigen-specific IgG subclass titers in mice immunized with DTaP. Antibody titers at wk14 of age were compared between C3H/HeJ (TLR4-deficient) and C3H/HeOuJ (TRL4-sufficient) mice. * p

    Journal: Vaccines

    Article Title: Genetic Variation in the Magnitude and Longevity of the IgG Subclass Response to a Diphtheria-Tetanus-Acellular Pertussis (DTaP) Vaccine in Mice

    doi: 10.3390/vaccines7040124

    Figure Lengend Snippet: Effect of TLR4 on the magnitude antigen-specific IgG subclass titers in mice immunized with DTaP. Antibody titers at wk14 of age were compared between C3H/HeJ (TLR4-deficient) and C3H/HeOuJ (TRL4-sufficient) mice. * p

    Article Snippet: The following are available online at https://www.mdpi.com/2076-393X/7/4/124/s1 , Figure S1: Longevity of IgG1, IgG2b and IgG3 to DTaP vaccine antigens in inbred mouse strains, Figure S2: IgG1, IgG2b and IgG3 titers at 24 weeks of age, 12 weeks after the last vaccination, Figure S3: Correlations between the magnitude of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S4: Correlations between the longevity of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S5: Correlations between the magnitude of IgG1 (a), IgG2b (b) and IgG3 (c) titers against different vaccine antigens, Figure S6: Correlations between the longevity of IgG1, IgG2b and IgG3 titers against different vaccine antigens, Figure S7: Venn diagrams for common genes associated with antibody titers and longevity, Figure S8: IgG1/IgG3 ratio against different vaccine antigens in BALB/cJ and C57BL/6J mice, Figure S9: Effect of TLR4-deficiency on the IgG1/IgG2a and IgG1/IgG3 ratios in mice immunized with DTaP.

    Techniques: Mouse Assay

    IgG1, IgG2b, and IgG3 titers to Diphtheria-Tetanus-Acellular Pertussis (DTaP) vaccine antigens in inbred mouse strains. Titers were determined at week 14 (two weeks after the third vaccination) against four antigens in the DTaP vaccine by ELISA. There was significant variation ( p

    Journal: Vaccines

    Article Title: Genetic Variation in the Magnitude and Longevity of the IgG Subclass Response to a Diphtheria-Tetanus-Acellular Pertussis (DTaP) Vaccine in Mice

    doi: 10.3390/vaccines7040124

    Figure Lengend Snippet: IgG1, IgG2b, and IgG3 titers to Diphtheria-Tetanus-Acellular Pertussis (DTaP) vaccine antigens in inbred mouse strains. Titers were determined at week 14 (two weeks after the third vaccination) against four antigens in the DTaP vaccine by ELISA. There was significant variation ( p

    Article Snippet: The following are available online at https://www.mdpi.com/2076-393X/7/4/124/s1 , Figure S1: Longevity of IgG1, IgG2b and IgG3 to DTaP vaccine antigens in inbred mouse strains, Figure S2: IgG1, IgG2b and IgG3 titers at 24 weeks of age, 12 weeks after the last vaccination, Figure S3: Correlations between the magnitude of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S4: Correlations between the longevity of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S5: Correlations between the magnitude of IgG1 (a), IgG2b (b) and IgG3 (c) titers against different vaccine antigens, Figure S6: Correlations between the longevity of IgG1, IgG2b and IgG3 titers against different vaccine antigens, Figure S7: Venn diagrams for common genes associated with antibody titers and longevity, Figure S8: IgG1/IgG3 ratio against different vaccine antigens in BALB/cJ and C57BL/6J mice, Figure S9: Effect of TLR4-deficiency on the IgG1/IgG2a and IgG1/IgG3 ratios in mice immunized with DTaP.

    Techniques: Enzyme-linked Immunosorbent Assay

    Ranking of mouse strains based on the mean of the IgG1/IgG3 ratios of the four vaccine antigens. A low IgG1/IgG3 ratio indicates Th1-prone and a high ratio indicates Th2-prone. NA: Antibodies induced against Prn in PWK/PhJ mice were below the cutoff and this was eliminated from the analysis.

    Journal: Vaccines

    Article Title: Genetic Variation in the Magnitude and Longevity of the IgG Subclass Response to a Diphtheria-Tetanus-Acellular Pertussis (DTaP) Vaccine in Mice

    doi: 10.3390/vaccines7040124

    Figure Lengend Snippet: Ranking of mouse strains based on the mean of the IgG1/IgG3 ratios of the four vaccine antigens. A low IgG1/IgG3 ratio indicates Th1-prone and a high ratio indicates Th2-prone. NA: Antibodies induced against Prn in PWK/PhJ mice were below the cutoff and this was eliminated from the analysis.

    Article Snippet: The following are available online at https://www.mdpi.com/2076-393X/7/4/124/s1 , Figure S1: Longevity of IgG1, IgG2b and IgG3 to DTaP vaccine antigens in inbred mouse strains, Figure S2: IgG1, IgG2b and IgG3 titers at 24 weeks of age, 12 weeks after the last vaccination, Figure S3: Correlations between the magnitude of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S4: Correlations between the longevity of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S5: Correlations between the magnitude of IgG1 (a), IgG2b (b) and IgG3 (c) titers against different vaccine antigens, Figure S6: Correlations between the longevity of IgG1, IgG2b and IgG3 titers against different vaccine antigens, Figure S7: Venn diagrams for common genes associated with antibody titers and longevity, Figure S8: IgG1/IgG3 ratio against different vaccine antigens in BALB/cJ and C57BL/6J mice, Figure S9: Effect of TLR4-deficiency on the IgG1/IgG2a and IgG1/IgG3 ratios in mice immunized with DTaP.

    Techniques: Mouse Assay

    Correlations between IgG1, IgG2b, and IgG3 against the same vaccine antigen. The correlations between IgG1, IgG2b, and IgG3 were plotted against the same vaccine antigen for antibody magnitude ( a ) and longevity ( b ). Mean value of the longevity of each strain was used to calculate for Pearson’s correlation coefficient (r) for the correlations between the magnitude and longevity of IgG1 and IgG2b, IgG1 and IgG3, IgG2b and IgG3. Significant values ( p

    Journal: Vaccines

    Article Title: Genetic Variation in the Magnitude and Longevity of the IgG Subclass Response to a Diphtheria-Tetanus-Acellular Pertussis (DTaP) Vaccine in Mice

    doi: 10.3390/vaccines7040124

    Figure Lengend Snippet: Correlations between IgG1, IgG2b, and IgG3 against the same vaccine antigen. The correlations between IgG1, IgG2b, and IgG3 were plotted against the same vaccine antigen for antibody magnitude ( a ) and longevity ( b ). Mean value of the longevity of each strain was used to calculate for Pearson’s correlation coefficient (r) for the correlations between the magnitude and longevity of IgG1 and IgG2b, IgG1 and IgG3, IgG2b and IgG3. Significant values ( p

    Article Snippet: The following are available online at https://www.mdpi.com/2076-393X/7/4/124/s1 , Figure S1: Longevity of IgG1, IgG2b and IgG3 to DTaP vaccine antigens in inbred mouse strains, Figure S2: IgG1, IgG2b and IgG3 titers at 24 weeks of age, 12 weeks after the last vaccination, Figure S3: Correlations between the magnitude of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S4: Correlations between the longevity of IgG1 and IgG2b (a), IgG1 and IgG3 (b), IgG2b and IgG3 (c) titers against the same vaccine antigen, Figure S5: Correlations between the magnitude of IgG1 (a), IgG2b (b) and IgG3 (c) titers against different vaccine antigens, Figure S6: Correlations between the longevity of IgG1, IgG2b and IgG3 titers against different vaccine antigens, Figure S7: Venn diagrams for common genes associated with antibody titers and longevity, Figure S8: IgG1/IgG3 ratio against different vaccine antigens in BALB/cJ and C57BL/6J mice, Figure S9: Effect of TLR4-deficiency on the IgG1/IgG2a and IgG1/IgG3 ratios in mice immunized with DTaP.

    Techniques:

    The anti-FIS IgM and IgG subclasses (IgG1 and IgG2) ELISA titres of cattle are vaccinated at week 0 and 3 with either PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and Emulsigen-D ® /Alhydrogel ® adjuvants (NegCtl) (n = 4) are presented as box and whisker plots. The cattle sera samples were collected before the vaccinations at week 0 and 3 as well as at week 5 and 12. Sera dilution started at a concentration of 1:50 and values below the (

    Journal: Pathogens

    Article Title: Immunogenicity of Non-Living Anthrax Vaccine Candidates in Cattle and Protective Efficacy of Immune Sera in A/J Mouse Model Compared to the Sterne Live Spore Vaccine

    doi: 10.3390/pathogens9070557

    Figure Lengend Snippet: The anti-FIS IgM and IgG subclasses (IgG1 and IgG2) ELISA titres of cattle are vaccinated at week 0 and 3 with either PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and Emulsigen-D ® /Alhydrogel ® adjuvants (NegCtl) (n = 4) are presented as box and whisker plots. The cattle sera samples were collected before the vaccinations at week 0 and 3 as well as at week 5 and 12. Sera dilution started at a concentration of 1:50 and values below the (

    Article Snippet: For IgG, the secondary antibody (goat anti-bovine IgG (ThermoFisher Scientific)) was diluted at the concentration of 1:10,000 in PTSMP, whereas for IgM, IgG1 and IgG2, sheep anti-bovine IgM (ThermoFisher Scientific), and sheep anti-bovine IgG1 and IgG2 (ThermoFisher Scientific) were all diluted at 1:4000 in PTSMP, respectively.

    Techniques: Enzyme-linked Immunosorbent Assay, Whisker Assay, Concentration Assay

    Anti-FIS IgG-titres in vaccinated cattle presented as box and whisker plots. The cattle were vaccinated twice at week 0 and 3 with PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and NegCtl (Emulsigen-D ® /Alhydrogel ® adjuvants) (n = 4) with sera collected before the vaccinations at week 0 and 3 as well as samples collected at week 5 and 12. Sera dilution started at a concentration of 1:100 and values

    Journal: Pathogens

    Article Title: Immunogenicity of Non-Living Anthrax Vaccine Candidates in Cattle and Protective Efficacy of Immune Sera in A/J Mouse Model Compared to the Sterne Live Spore Vaccine

    doi: 10.3390/pathogens9070557

    Figure Lengend Snippet: Anti-FIS IgG-titres in vaccinated cattle presented as box and whisker plots. The cattle were vaccinated twice at week 0 and 3 with PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and NegCtl (Emulsigen-D ® /Alhydrogel ® adjuvants) (n = 4) with sera collected before the vaccinations at week 0 and 3 as well as samples collected at week 5 and 12. Sera dilution started at a concentration of 1:100 and values

    Article Snippet: For IgG, the secondary antibody (goat anti-bovine IgG (ThermoFisher Scientific)) was diluted at the concentration of 1:10,000 in PTSMP, whereas for IgM, IgG1 and IgG2, sheep anti-bovine IgM (ThermoFisher Scientific), and sheep anti-bovine IgG1 and IgG2 (ThermoFisher Scientific) were all diluted at 1:4000 in PTSMP, respectively.

    Techniques: Whisker Assay, Concentration Assay

    The anti-rPA IgM and IgG subclasses (IgG1 and IgG2) ELISA titres of cattle are vaccinated at week 0 and 3 with either PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and Emulsigen-D ® /Alhydrogel ® adjuvants (NegCtl) (n = 4) are presented as box and whisker plots. The cattle sera samples were collected before the vaccinations at week 0 and 3 as well as at week 5 and 12. Sera dilution started at a concentration of 1:50 and values below the (

    Journal: Pathogens

    Article Title: Immunogenicity of Non-Living Anthrax Vaccine Candidates in Cattle and Protective Efficacy of Immune Sera in A/J Mouse Model Compared to the Sterne Live Spore Vaccine

    doi: 10.3390/pathogens9070557

    Figure Lengend Snippet: The anti-rPA IgM and IgG subclasses (IgG1 and IgG2) ELISA titres of cattle are vaccinated at week 0 and 3 with either PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), SLSV (n = 8) and Emulsigen-D ® /Alhydrogel ® adjuvants (NegCtl) (n = 4) are presented as box and whisker plots. The cattle sera samples were collected before the vaccinations at week 0 and 3 as well as at week 5 and 12. Sera dilution started at a concentration of 1:50 and values below the (

    Article Snippet: For IgG, the secondary antibody (goat anti-bovine IgG (ThermoFisher Scientific)) was diluted at the concentration of 1:10,000 in PTSMP, whereas for IgM, IgG1 and IgG2, sheep anti-bovine IgM (ThermoFisher Scientific), and sheep anti-bovine IgG1 and IgG2 (ThermoFisher Scientific) were all diluted at 1:4000 in PTSMP, respectively.

    Techniques: Recombinase Polymerase Amplification, Enzyme-linked Immunosorbent Assay, Whisker Assay, Concentration Assay

    The cumulative mice survival curve following passive in vivo transfer of polyclonal IgG purified from cattle immune sera A/J mice. The mice were lethally challenged with 2.16 × 10 5 B. anthracis 34F2 spores. The sera are from cattle vaccinated twice (week 0 and 3) with either PrPA+FIS, CrPA+FIS, SLSV groups adjuvanted with emulsigen-D ® /alhydrogel ® and a NegCtl group (also see Table 2 for the number of mice allocated to each group). The survival rate in the Log-rank (Mantel–Cox) test was compared to the NegCtl group. The significant values between groups are presented as **** p

    Journal: Pathogens

    Article Title: Immunogenicity of Non-Living Anthrax Vaccine Candidates in Cattle and Protective Efficacy of Immune Sera in A/J Mouse Model Compared to the Sterne Live Spore Vaccine

    doi: 10.3390/pathogens9070557

    Figure Lengend Snippet: The cumulative mice survival curve following passive in vivo transfer of polyclonal IgG purified from cattle immune sera A/J mice. The mice were lethally challenged with 2.16 × 10 5 B. anthracis 34F2 spores. The sera are from cattle vaccinated twice (week 0 and 3) with either PrPA+FIS, CrPA+FIS, SLSV groups adjuvanted with emulsigen-D ® /alhydrogel ® and a NegCtl group (also see Table 2 for the number of mice allocated to each group). The survival rate in the Log-rank (Mantel–Cox) test was compared to the NegCtl group. The significant values between groups are presented as **** p

    Article Snippet: For IgG, the secondary antibody (goat anti-bovine IgG (ThermoFisher Scientific)) was diluted at the concentration of 1:10,000 in PTSMP, whereas for IgM, IgG1 and IgG2, sheep anti-bovine IgM (ThermoFisher Scientific), and sheep anti-bovine IgG1 and IgG2 (ThermoFisher Scientific) were all diluted at 1:4000 in PTSMP, respectively.

    Techniques: Mouse Assay, In Vivo, Purification

    Anti-recombinant protective antigen (rPA) IgG-titres in vaccinated cattle presented as box and whisker plots. The cattle were vaccinated twice at week 0 and 3 with PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), Sterne live spore vaccine (SLSV) (n = 8) and NegCtl (Emulsigen-D ® /Alhydrogel ® adjuvants) (n = 4) with sera collected before the vaccinations at week 0 and 3 as well as samples collected at week 5 and 12. Sera dilution started at a concentration of 1:100 and values

    Journal: Pathogens

    Article Title: Immunogenicity of Non-Living Anthrax Vaccine Candidates in Cattle and Protective Efficacy of Immune Sera in A/J Mouse Model Compared to the Sterne Live Spore Vaccine

    doi: 10.3390/pathogens9070557

    Figure Lengend Snippet: Anti-recombinant protective antigen (rPA) IgG-titres in vaccinated cattle presented as box and whisker plots. The cattle were vaccinated twice at week 0 and 3 with PrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), CrPA+FIS+Emulsigen-D ® /Alhydrogel ® adjuvants (n = 8), Sterne live spore vaccine (SLSV) (n = 8) and NegCtl (Emulsigen-D ® /Alhydrogel ® adjuvants) (n = 4) with sera collected before the vaccinations at week 0 and 3 as well as samples collected at week 5 and 12. Sera dilution started at a concentration of 1:100 and values

    Article Snippet: For IgG, the secondary antibody (goat anti-bovine IgG (ThermoFisher Scientific)) was diluted at the concentration of 1:10,000 in PTSMP, whereas for IgM, IgG1 and IgG2, sheep anti-bovine IgM (ThermoFisher Scientific), and sheep anti-bovine IgG1 and IgG2 (ThermoFisher Scientific) were all diluted at 1:4000 in PTSMP, respectively.

    Techniques: Recombinant, Recombinase Polymerase Amplification, Whisker Assay, Concentration Assay