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  • 86
    MultiSciences Biotech Co Ltd human ifn γ elisa kit
    The specific cellular immunity tested by ELISA. The PBMCs of the HC group and the CE group were separated and co-cultured with the vaccine designed in this study. The CD4 + T-cell immune response was evaluated by detecting the secretion of <t>IFN-γ</t> in the culture supernatant and the CD8 + T-cell immune response was evaluated by detecting the secretion of Perforin and Granzyme-B. (A) The relative level of IFN-γ in the HC group and the CE group. (B) The relative level of Perforin in the HC group and the CE group. (C) The relative level of Granzyme-B in the HC group and the CE group. The relative level of CE group was higher than that of HC group. (*** P < 0.001).
    Human Ifn γ Elisa Kit, supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human ifn γ elisa kit/product/MultiSciences Biotech Co Ltd
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human ifn γ elisa kit - by Bioz Stars, 2023-01
    86/100 stars
      Buy from Supplier

    86
    Signalway Antibody mouse malonyl coa elisa kit
    Effect of LNP- Lbp UNA-siRNA treatment on liver lipid accumulation and lipogenesis induced by an HFHS diet (A–P) Effects of 6-months LNP- Lbp UNA-siRNA administration on representative standard Masson’s trichrome staining histological liver slides (20×), lipid droplet (LD) count and area (A and I), liver triglycerides (B and <t>J),</t> <t>malonyl-CoA</t> levels (C and K), expression of lipogenic (D and L) genes, FAS and ACC protein (E, F, M, and N), ACC activity (G and O), and MDA levels (H and P) in HFHS-fed male (A–H) and female (I–P) mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.
    Mouse Malonyl Coa Elisa Kit, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse malonyl coa elisa kit/product/Signalway Antibody
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse malonyl coa elisa kit - by Bioz Stars, 2023-01
    86/100 stars
      Buy from Supplier



    Image Search Results


    The specific cellular immunity tested by ELISA. The PBMCs of the HC group and the CE group were separated and co-cultured with the vaccine designed in this study. The CD4 + T-cell immune response was evaluated by detecting the secretion of IFN-γ in the culture supernatant and the CD8 + T-cell immune response was evaluated by detecting the secretion of Perforin and Granzyme-B. (A) The relative level of IFN-γ in the HC group and the CE group. (B) The relative level of Perforin in the HC group and the CE group. (C) The relative level of Granzyme-B in the HC group and the CE group. The relative level of CE group was higher than that of HC group. (*** P < 0.001).

    Journal: Frontiers in Immunology

    Article Title: Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics

    doi: 10.3389/fimmu.2021.668492

    Figure Lengend Snippet: The specific cellular immunity tested by ELISA. The PBMCs of the HC group and the CE group were separated and co-cultured with the vaccine designed in this study. The CD4 + T-cell immune response was evaluated by detecting the secretion of IFN-γ in the culture supernatant and the CD8 + T-cell immune response was evaluated by detecting the secretion of Perforin and Granzyme-B. (A) The relative level of IFN-γ in the HC group and the CE group. (B) The relative level of Perforin in the HC group and the CE group. (C) The relative level of Granzyme-B in the HC group and the CE group. The relative level of CE group was higher than that of HC group. (*** P < 0.001).

    Article Snippet: The Human IFN-γ ELISA Kit (70-EK180-48, MultiSciences, Hangzhou, China) was adopted for the ELISA experiment.

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture

    Effect of LNP- Lbp UNA-siRNA treatment on liver lipid accumulation and lipogenesis induced by an HFHS diet (A–P) Effects of 6-months LNP- Lbp UNA-siRNA administration on representative standard Masson’s trichrome staining histological liver slides (20×), lipid droplet (LD) count and area (A and I), liver triglycerides (B and J), malonyl-CoA levels (C and K), expression of lipogenic (D and L) genes, FAS and ACC protein (E, F, M, and N), ACC activity (G and O), and MDA levels (H and P) in HFHS-fed male (A–H) and female (I–P) mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

    doi: 10.1016/j.omtn.2022.08.003

    Figure Lengend Snippet: Effect of LNP- Lbp UNA-siRNA treatment on liver lipid accumulation and lipogenesis induced by an HFHS diet (A–P) Effects of 6-months LNP- Lbp UNA-siRNA administration on representative standard Masson’s trichrome staining histological liver slides (20×), lipid droplet (LD) count and area (A and I), liver triglycerides (B and J), malonyl-CoA levels (C and K), expression of lipogenic (D and L) genes, FAS and ACC protein (E, F, M, and N), ACC activity (G and O), and MDA levels (H and P) in HFHS-fed male (A–H) and female (I–P) mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.

    Article Snippet: Liver (∼20 mg) was homogenized in PBS and centrifuged for 20 min at 2,000–3,000 RPM, and supernatant was used to measure malonyl-CoA levels using Mouse Malonyl-CoA ELISA KIT (cat. no. EK18900, Signalway Antibody, Greenbelt, MD, USA) and strictly following the manufacture’s protocol.

    Techniques: Staining, Expressing, Activity Assay

    Result of LNP- Lbp UNA-siRNA treatment on liver lipid accumulation and lipogenesis in mice with established obesity (A–P) Effects of 8-weeks LNP- Lbp UNA-siRNA administration on Lbp mRNA levels (A and I), representative standard Masson’s trichrome staining histological liver slides (20×), LD count and area (B and J), liver triglycerides (C and K), malonyl-CoA levels (D and L), expression of lipogenic (E and M) genes, ACC protein (F and N), ACC activity (G and O), and MDA levels (H and P) in diet-induced obese male (A–H) and female (I–P) mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with vehicle-treated obese mice.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

    doi: 10.1016/j.omtn.2022.08.003

    Figure Lengend Snippet: Result of LNP- Lbp UNA-siRNA treatment on liver lipid accumulation and lipogenesis in mice with established obesity (A–P) Effects of 8-weeks LNP- Lbp UNA-siRNA administration on Lbp mRNA levels (A and I), representative standard Masson’s trichrome staining histological liver slides (20×), LD count and area (B and J), liver triglycerides (C and K), malonyl-CoA levels (D and L), expression of lipogenic (E and M) genes, ACC protein (F and N), ACC activity (G and O), and MDA levels (H and P) in diet-induced obese male (A–H) and female (I–P) mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with vehicle-treated obese mice.

    Article Snippet: Liver (∼20 mg) was homogenized in PBS and centrifuged for 20 min at 2,000–3,000 RPM, and supernatant was used to measure malonyl-CoA levels using Mouse Malonyl-CoA ELISA KIT (cat. no. EK18900, Signalway Antibody, Greenbelt, MD, USA) and strictly following the manufacture’s protocol.

    Techniques: Staining, Expressing, Activity Assay

    LNP- Lbp UNA-siRNA treatment effects on liver lipogenesis and triglycerides in mice fed with standard diet (STD) and MCD (A–I) Effects of weekly LNP- Lbp UNA-siRNA administration in STD- and MCD-fed mice on liver Lbp and lipogenesis ( Fasn , Srebf1 , and Scd1 )-related gene expression (A and B), malonyl-CoA (C), FAS and ACC protein levels (D and E), ACC activity (F), NADPH production ( Idh1 , Me1 )-related gene expression (G), MDA (H), and triglycerides levels (I). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with vehicle; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with STD. siC, weekly LNP-non-targeting UNA-siRNA (3 mg/kg)-treated mice; siLbp, weekly LNP- Lbp UNA-siRNA (3 mg/kg)-treated mice.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

    doi: 10.1016/j.omtn.2022.08.003

    Figure Lengend Snippet: LNP- Lbp UNA-siRNA treatment effects on liver lipogenesis and triglycerides in mice fed with standard diet (STD) and MCD (A–I) Effects of weekly LNP- Lbp UNA-siRNA administration in STD- and MCD-fed mice on liver Lbp and lipogenesis ( Fasn , Srebf1 , and Scd1 )-related gene expression (A and B), malonyl-CoA (C), FAS and ACC protein levels (D and E), ACC activity (F), NADPH production ( Idh1 , Me1 )-related gene expression (G), MDA (H), and triglycerides levels (I). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with vehicle; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with STD. siC, weekly LNP-non-targeting UNA-siRNA (3 mg/kg)-treated mice; siLbp, weekly LNP- Lbp UNA-siRNA (3 mg/kg)-treated mice.

    Article Snippet: Liver (∼20 mg) was homogenized in PBS and centrifuged for 20 min at 2,000–3,000 RPM, and supernatant was used to measure malonyl-CoA levels using Mouse Malonyl-CoA ELISA KIT (cat. no. EK18900, Signalway Antibody, Greenbelt, MD, USA) and strictly following the manufacture’s protocol.

    Techniques: Expressing, Activity Assay