Journal: Frontiers in Immunology

Article Title: Design of a Novel Multi-Epitope Vaccine Against Echinococcus granulosus in Immunoinformatics

doi: 10.3389/fimmu.2021.668492

Figure Lengend Snippet: The specific cellular immunity tested by ELISA. The PBMCs of the HC group and the CE group were separated and co-cultured with the vaccine designed in this study. The CD4 + T-cell immune response was evaluated by detecting the secretion of IFN-γ in the culture supernatant and the CD8 + T-cell immune response was evaluated by detecting the secretion of Perforin and Granzyme-B. (A) The relative level of IFN-γ in the HC group and the CE group. (B) The relative level of Perforin in the HC group and the CE group. (C) The relative level of Granzyme-B in the HC group and the CE group. The relative level of CE group was higher than that of HC group. (*** P < 0.001).

Article Snippet: The Human IFN-γ ELISA Kit (70-EK180-48, MultiSciences, Hangzhou, China) was adopted for the ELISA experiment.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: Molecular Therapy. Nucleic Acids

Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

doi: 10.1016/j.omtn.2022.08.003

Figure Lengend Snippet: Effect of LNP- Lbp UNA-siRNA treatment on liver lipid accumulation and lipogenesis induced by an HFHS diet (A–P) Effects of 6-months LNP- Lbp UNA-siRNA administration on representative standard Masson’s trichrome staining histological liver slides (20×), lipid droplet (LD) count and area (A and I), liver triglycerides (B and J), malonyl-CoA levels (C and K), expression of lipogenic (D and L) genes, FAS and ACC protein (E, F, M, and N), ACC activity (G and O), and MDA levels (H and P) in HFHS-fed male (A–H) and female (I–P) mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CD; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with HFHS.

Article Snippet: Liver (∼20 mg) was homogenized in PBS and centrifuged for 20 min at 2,000–3,000 RPM, and supernatant was used to measure malonyl-CoA levels using Mouse Malonyl-CoA ELISA KIT (cat. no. EK18900, Signalway Antibody, Greenbelt, MD, USA) and strictly following the manufacture’s protocol.

Techniques: Staining, Expressing, Activity Assay

Journal: Molecular Therapy. Nucleic Acids

Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

doi: 10.1016/j.omtn.2022.08.003

Figure Lengend Snippet: Result of LNP- Lbp UNA-siRNA treatment on liver lipid accumulation and lipogenesis in mice with established obesity (A–P) Effects of 8-weeks LNP- Lbp UNA-siRNA administration on Lbp mRNA levels (A and I), representative standard Masson’s trichrome staining histological liver slides (20×), LD count and area (B and J), liver triglycerides (C and K), malonyl-CoA levels (D and L), expression of lipogenic (E and M) genes, ACC protein (F and N), ACC activity (G and O), and MDA levels (H and P) in diet-induced obese male (A–H) and female (I–P) mice. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with vehicle-treated obese mice.

Article Snippet: Liver (∼20 mg) was homogenized in PBS and centrifuged for 20 min at 2,000–3,000 RPM, and supernatant was used to measure malonyl-CoA levels using Mouse Malonyl-CoA ELISA KIT (cat. no. EK18900, Signalway Antibody, Greenbelt, MD, USA) and strictly following the manufacture’s protocol.

Techniques: Staining, Expressing, Activity Assay

Journal: Molecular Therapy. Nucleic Acids

Article Title: Downregulation of hepatic lipopolysaccharide binding protein improves lipogenesis-induced liver lipid accumulation

doi: 10.1016/j.omtn.2022.08.003

Figure Lengend Snippet: LNP- Lbp UNA-siRNA treatment effects on liver lipogenesis and triglycerides in mice fed with standard diet (STD) and MCD (A–I) Effects of weekly LNP- Lbp UNA-siRNA administration in STD- and MCD-fed mice on liver Lbp and lipogenesis ( Fasn , Srebf1 , and Scd1 )-related gene expression (A and B), malonyl-CoA (C), FAS and ACC protein levels (D and E), ACC activity (F), NADPH production ( Idh1 , Me1 )-related gene expression (G), MDA (H), and triglycerides levels (I). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with vehicle; † p < 0.05, †† p < 0.01, and ††† p < 0.001 compared with STD. siC, weekly LNP-non-targeting UNA-siRNA (3 mg/kg)-treated mice; siLbp, weekly LNP- Lbp UNA-siRNA (3 mg/kg)-treated mice.

Article Snippet: Liver (∼20 mg) was homogenized in PBS and centrifuged for 20 min at 2,000–3,000 RPM, and supernatant was used to measure malonyl-CoA levels using Mouse Malonyl-CoA ELISA KIT (cat. no. EK18900, Signalway Antibody, Greenbelt, MD, USA) and strictly following the manufacture’s protocol.

Techniques: Expressing, Activity Assay