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    Boster Bio mouse tnfsf11 rankl picokine elisa kit
    <t>OPG/RANKL</t> ratio in OBs/OCLs co-culture. In the graph is reported the ratio of the soluble factors measured by <t>ELISA</t> kit. Mean and standard error of three replicates are shown. Statistical significant differences among the samples are indicated in the graph: *p≤0.05.
    Mouse Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-12
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    OPG/RANKL ratio in OBs/OCLs co-culture. In the graph is reported the ratio of the soluble factors measured by ELISA kit. Mean and standard error of three replicates are shown. Statistical significant differences among the samples are indicated in the graph: *p≤0.05.

    Journal: PLoS ONE

    Article Title: Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis

    doi: 10.1371/journal.pone.0132633

    Figure Lengend Snippet: OPG/RANKL ratio in OBs/OCLs co-culture. In the graph is reported the ratio of the soluble factors measured by ELISA kit. Mean and standard error of three replicates are shown. Statistical significant differences among the samples are indicated in the graph: *p≤0.05.

    Article Snippet: RANKL was detected by using Mouse OPG ELISA Kit and Mouse TNFSF11/RANKL ELISA Kit (Boster Biological Technology, Fremont, CA).

    Techniques: Co-Culture Assay, Enzyme-linked Immunosorbent Assay

    Dopaminergic neurons suppress osteoclastogenesis. ( A ) The percentage of osteoclast precursor cells, characterized by the expression of the cell surface markers c-kit and c-fms, together with the absence or dull expression of CD11b, in bone marrow of control and MPTP-injected mice. Representative data (left) and quantification (right, control, n = 8; MPTP, n = 8) are shown. ( B ) Osteoclast differentiation of BMMs obtained from control and MPTP-injected mice. Left: representative images obtained from more than three independent experiments. Right: quantification of TRAP + multinuclear osteoclasts (MNC) (control, n = 6; MPTP, n = 6). ( C ) mRNA expression of NFATc1 of cells obtained from control and MPTP-injected mice 2 days after RANKL stimulation (control, n = 8; MPTP, n = 8). ( D ) Osteoclast differentiation in presence of 2% serum obtained from control and MPTP-injected mice and the effect of anti-prolactin neutralizing antibody in the presence of MPTP serum (left). Left: representative images obtained more than three independent experiments. Right: quantification of TRAP + MNC (control, n = 6; MPTP, n = 6; MPTP+anti-prolactin Ab, n = 6). ( E ) mRNA expression of NFATc1 of cells 2 days after RANKL stimulation in the presence of mouse serum (control, n = 8; MPTP, n = 8). ( F ) Ratio of RANKL/OPG in serum from control and MPTP-injected mice (control, n = 8; MPTP, n = 8). Statistical analyses were performed using Student’s t-test ( A – C , E , F ) or ANOVA with Dunnett’s multiple-comparison test ( D ). * P

    Journal: Scientific Reports

    Article Title: Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson’s disease model mice

    doi: 10.1038/s41598-019-50336-4

    Figure Lengend Snippet: Dopaminergic neurons suppress osteoclastogenesis. ( A ) The percentage of osteoclast precursor cells, characterized by the expression of the cell surface markers c-kit and c-fms, together with the absence or dull expression of CD11b, in bone marrow of control and MPTP-injected mice. Representative data (left) and quantification (right, control, n = 8; MPTP, n = 8) are shown. ( B ) Osteoclast differentiation of BMMs obtained from control and MPTP-injected mice. Left: representative images obtained from more than three independent experiments. Right: quantification of TRAP + multinuclear osteoclasts (MNC) (control, n = 6; MPTP, n = 6). ( C ) mRNA expression of NFATc1 of cells obtained from control and MPTP-injected mice 2 days after RANKL stimulation (control, n = 8; MPTP, n = 8). ( D ) Osteoclast differentiation in presence of 2% serum obtained from control and MPTP-injected mice and the effect of anti-prolactin neutralizing antibody in the presence of MPTP serum (left). Left: representative images obtained more than three independent experiments. Right: quantification of TRAP + MNC (control, n = 6; MPTP, n = 6; MPTP+anti-prolactin Ab, n = 6). ( E ) mRNA expression of NFATc1 of cells 2 days after RANKL stimulation in the presence of mouse serum (control, n = 8; MPTP, n = 8). ( F ) Ratio of RANKL/OPG in serum from control and MPTP-injected mice (control, n = 8; MPTP, n = 8). Statistical analyses were performed using Student’s t-test ( A – C , E , F ) or ANOVA with Dunnett’s multiple-comparison test ( D ). * P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The concentrations of RANKL, OPG and Hcy in obtained mouse serum were determined using a Mouse TNFSF11/RANKL EZ-Set™ ELISA Kit, Mouse OPG (TNFRSF11B) PicoKine™ ELISA Kit (Boster Biological Technology) and Hcy ELISA Kit (Cosmo Bio), respectively, according to the individual manufacturer’s instructions.

    Techniques: Expressing, Injection, Mouse Assay