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  • 92
    Boster Bio mice ptx3 elisa kit
    Mice Ptx3 Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Syd Labs human smica protein
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    Boster Bio serum kim 1
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    94
    Boster Bio tnfsf11 rankl picokine elisa kit
    Adipocytes are the principal source for <t>RANKL</t> after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H&E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H&E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Redox Biology

    Article Title: IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism

    doi: 10.1016/j.redox.2022.102373

    Figure Lengend Snippet: Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H&E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Serum levels of OCN, OPG, RANKL and insulin were measured by mouse Osteocalcin ELISA Kit (BioVision), OPG ELISA Kit (Boster Biological Technology), TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) and Ultra-Sensitive Insulin ELISA Kit (Crystal Chem), respectively, according to the manufacturer's instructions.

    Techniques: Expressing, Staining, Quantitative RT-PCR, Micro-CT