Journal: Journal of Orthopaedic Surgery and Research
Article Title: Astaxanthin alleviates spinal cord ischemia-reperfusion injury via activation of PI3K/Akt/GSK-3β pathway in rats
Figure Lengend Snippet: AST partially inhibited proinflammatory cytokines at 72 h after SCII. The levels of IL-1β ( a ), TNF-α ( b ), and IL-18 ( c ) were substantially increased, and IL-10 ( d ) was decreased in the SCII group compared with the sham group. Although pretreatment with AST could moderately reverse the above changes in cytokines, only the level of TNF-α was significantly decreased. Values are mean ± SD ( n = 6/group). * P
Article Snippet: Determination of inflammatory factorsThe levels of IL-1β (RLB00), TNF-α (RTA00), and IL-10 (R1000) (R & D Systems, Minneapolis, MN, USA) as well as IL-18 (EK0592, Boster Biological Technology, Pleasanton, CA, USA) were determined by ELISA kits according to the manufacturer’s instructions.
Techniques: AST Assay