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  • 99
    New England Biolabs golden gate assembly
    Molbit design scheme. (a) Molecular bit (molbit) structure. The molbit sequence is attached to a spacer sequence via <t>Golden</t> <t>Gate</t> <t>assembly</t> to achieve a minimum length for sequencing and provide an additional encoding channel. Since the sequencing adapter is attached to both ends, the strand can be sensed from either direction. (b) The letters “UW” depicted visually in nanopore raw data (as opposed to encoded in the sequence contents). From top to bottom, the shown sequence was simulated using Scrappie and sequenced on the ONT Min-ION, demonstrating the viability of using simulations for designing intentional, arbitrary raw signal shapes. (c) Evolutionary model workflow. Each round of evolution begins with a set of sequences, their simulated squiggles, and pairwise Dynamic Time Warping (DTW) distances. The sequence order is randomized, and sequences are mutated one at a time, verifying DTW improvement (minimum and mean) after each attempt. (d) Dynamic time warping (DTW) scores before (left) and after (right) 31 iterations of the evolutionary model. After initialization, the minimum DTW similarity was 2.9 (mean 4.2 +/- 0.4), and after evolution the minimum was 4.2 (mean 5.8 +/- 0.8).
    Golden Gate Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/golden gate assembly/product/New England Biolabs
    Average 99 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    golden gate assembly - by Bioz Stars, 2020-08
    99/100 stars
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    91
    Nikon nikon e1600 microscope
    Effect of injury on vaginal elastic fibers with injury ± actinonin. Microscopic images of vaginal muscularis on 3d postpartum in ( A ) injury + PBS ( Inj 3d ) and injury + actinonin ( Inj + Act 3d ). The 4–5 µm sections of full-thickness vaginal rings were stained with Hart’s stain. Quantitative analysis performed using a Nikon <t>E1600</t> microscope with 40x objective and ImageJ software. Scale bars 50 µm. Graphical depiction represents ( B ) elastin fiber length (µm) and ( C ) percent area covered by elastic fibers in pregnant ( D21 ) controls compared to postpartum time points for injury with (triangles) and without actinonin (solid circles). n = 4–5 in all groups, *P
    Nikon E1600 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nikon e1600 microscope/product/Nikon
    Average 91 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    nikon e1600 microscope - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    90
    Nikon nikon e1600 microsocpe
    Effect of injury on vaginal elastic fibers with injury ± actinonin. Microscopic images of vaginal muscularis on 3d postpartum in ( A ) injury + PBS ( Inj 3d ) and injury + actinonin ( Inj + Act 3d ). The 4–5 µm sections of full-thickness vaginal rings were stained with Hart’s stain. Quantitative analysis performed using a Nikon <t>E1600</t> microscope with 40x objective and ImageJ software. Scale bars 50 µm. Graphical depiction represents ( B ) elastin fiber length (µm) and ( C ) percent area covered by elastic fibers in pregnant ( D21 ) controls compared to postpartum time points for injury with (triangles) and without actinonin (solid circles). n = 4–5 in all groups, *P
    Nikon E1600 Microsocpe, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nikon e1600 microsocpe/product/Nikon
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nikon e1600 microsocpe - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Molbit design scheme. (a) Molecular bit (molbit) structure. The molbit sequence is attached to a spacer sequence via Golden Gate assembly to achieve a minimum length for sequencing and provide an additional encoding channel. Since the sequencing adapter is attached to both ends, the strand can be sensed from either direction. (b) The letters “UW” depicted visually in nanopore raw data (as opposed to encoded in the sequence contents). From top to bottom, the shown sequence was simulated using Scrappie and sequenced on the ONT Min-ION, demonstrating the viability of using simulations for designing intentional, arbitrary raw signal shapes. (c) Evolutionary model workflow. Each round of evolution begins with a set of sequences, their simulated squiggles, and pairwise Dynamic Time Warping (DTW) distances. The sequence order is randomized, and sequences are mutated one at a time, verifying DTW improvement (minimum and mean) after each attempt. (d) Dynamic time warping (DTW) scores before (left) and after (right) 31 iterations of the evolutionary model. After initialization, the minimum DTW similarity was 2.9 (mean 4.2 +/- 0.4), and after evolution the minimum was 4.2 (mean 5.8 +/- 0.8).

    Journal: bioRxiv

    Article Title: Porcupine: Rapid and robust tagging of physical objects using nanopore-orthogonal DNA strands

    doi: 10.1101/2020.03.06.981514

    Figure Lengend Snippet: Molbit design scheme. (a) Molecular bit (molbit) structure. The molbit sequence is attached to a spacer sequence via Golden Gate assembly to achieve a minimum length for sequencing and provide an additional encoding channel. Since the sequencing adapter is attached to both ends, the strand can be sensed from either direction. (b) The letters “UW” depicted visually in nanopore raw data (as opposed to encoded in the sequence contents). From top to bottom, the shown sequence was simulated using Scrappie and sequenced on the ONT Min-ION, demonstrating the viability of using simulations for designing intentional, arbitrary raw signal shapes. (c) Evolutionary model workflow. Each round of evolution begins with a set of sequences, their simulated squiggles, and pairwise Dynamic Time Warping (DTW) distances. The sequence order is randomized, and sequences are mutated one at a time, verifying DTW improvement (minimum and mean) after each attempt. (d) Dynamic time warping (DTW) scores before (left) and after (right) 31 iterations of the evolutionary model. After initialization, the minimum DTW similarity was 2.9 (mean 4.2 +/- 0.4), and after evolution the minimum was 4.2 (mean 5.8 +/- 0.8).

    Article Snippet: To assemble the molbits, 600 ng of the desired annealed barcodes (at equimolar concentrations) and 600 ng of the spacer were ligated together using NEB’s Golden Gate Assembly Kit.

    Techniques: Sequencing

    Effect of injury on vaginal elastic fibers with injury ± actinonin. Microscopic images of vaginal muscularis on 3d postpartum in ( A ) injury + PBS ( Inj 3d ) and injury + actinonin ( Inj + Act 3d ). The 4–5 µm sections of full-thickness vaginal rings were stained with Hart’s stain. Quantitative analysis performed using a Nikon E1600 microscope with 40x objective and ImageJ software. Scale bars 50 µm. Graphical depiction represents ( B ) elastin fiber length (µm) and ( C ) percent area covered by elastic fibers in pregnant ( D21 ) controls compared to postpartum time points for injury with (triangles) and without actinonin (solid circles). n = 4–5 in all groups, *P

    Journal: Scientific Reports

    Article Title: Protease Inhibition Improves Healing of The Vaginal Wall after Obstetrical Injury: Results from a Preclinical Animal Model

    doi: 10.1038/s41598-020-63031-6

    Figure Lengend Snippet: Effect of injury on vaginal elastic fibers with injury ± actinonin. Microscopic images of vaginal muscularis on 3d postpartum in ( A ) injury + PBS ( Inj 3d ) and injury + actinonin ( Inj + Act 3d ). The 4–5 µm sections of full-thickness vaginal rings were stained with Hart’s stain. Quantitative analysis performed using a Nikon E1600 microscope with 40x objective and ImageJ software. Scale bars 50 µm. Graphical depiction represents ( B ) elastin fiber length (µm) and ( C ) percent area covered by elastic fibers in pregnant ( D21 ) controls compared to postpartum time points for injury with (triangles) and without actinonin (solid circles). n = 4–5 in all groups, *P

    Article Snippet: Images of each Masson trichrome section were captured and analyzed using a Nikon E1600 microscope and Nikon NIS Elements AR software (Melville, NY).

    Techniques: Staining, Microscopy, Software