Journal: Cell Stress & Chaperones

Article Title: Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways

doi: 10.1007/s12192-015-0654-4

Figure Lengend Snippet: Salidroside inhibits BLM-induced EMT in rat lung tissues. a Immunofluorescent staining was performed in rat lung tissue slices by using a specific antibody against E-cadherin (red). DAPI was used to visualize the cell nuclei (blue). Bars = 50 μM. b The mRNA expression levels of E-cadherin were determined with real-time quantitative RT-PCR. β-actin was used as the endogenous control. c Protein expression levels of E-cadherin, fibronectin, vimentin, and α-SMA were analyzed with Western blot assay and d calculated as ratios to that of β-actin in the same sample. The data were presented as mean ± standard deviation (SD). ††† P < 0.001 was compared to the control group; ns P > 0.05, *P < 0.05, **P < 0.01, and ***P < 0.001 were compared to the BLM group

Article Snippet: Both cell and tissue samples were blocked with goat serum and then incubated with rabbit polyclonal antibodies for E-cadherin (1:100 diluted for tissues and 1:200 diluted for cells; Boster, Wuhan, China) and fibronectin (1:200 diluted for cells; Boster) overnight (4 °C).

Techniques: Staining, Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation

Journal: Cell Stress & Chaperones

Article Title: Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways

doi: 10.1007/s12192-015-0654-4

Figure Lengend Snippet: Salidroside blocks recombinant TGF-β1-induced EMT-like shift in AECs in vitro. Recombinant TGF-β1 (5 or 10 ng/mL) and salidroside (50 μM) were utilized to co-treat the primarily cultured rat AECs or human A549 cells for 48 h. a–d Then, these cells were subjected to immunohistochemical analysis using antibodies against E-cadherin (green) and fibronectin (red). Cell nuclei were stained with DAPI (blue). e, f Protein expression levels of E-cadherin and fibronectin were analyzed with Western blot assay. β-Actin was used as the endogenous control

Article Snippet: Both cell and tissue samples were blocked with goat serum and then incubated with rabbit polyclonal antibodies for E-cadherin (1:100 diluted for tissues and 1:200 diluted for cells; Boster, Wuhan, China) and fibronectin (1:200 diluted for cells; Boster) overnight (4 °C).

Techniques: Recombinant, In Vitro, Cell Culture, Immunohistochemical staining, Staining, Expressing, Western Blot

Journal: Biological Research

Article Title: Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture

doi: 10.1186/s40659-015-0015-x

Figure Lengend Snippet: Microscopic morphologies of the microbial cells in mixed culture media (A) C. acetobutylicum ATCC 824 and E. cloacae ATCC 13047 in H1 medium; (B) C. acetobutylicum ATCC 824 in H2 medium.

Article Snippet: For the two-step biohydrogen production experiment, the H1 medium, after cultured the microbial strains E. cloacae ATCC 13047 and K. marxianus 15D, was centrifuged to remove the microbial cells and then mixed with GAM broth (H2 medium).

Techniques: