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    Thermo Fisher schneider s drosophila medium
    Schneider S Drosophila Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher drosophila s2 cells
    Drosophila S2 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Developmental Studies Hybridoma Bank bloomington drosophila stock center
    Bloomington Drosophila Stock Center, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher s2 cells
    Effect of selected RNAi treatments on Nef-induced CD4 downregulation in stably transfected S2 cells. (A) CD4 expression profiles of <t>S2</t> cells stably expressing CD4 and Nef, after treatment with dsRNAs targeting GFP (nontargeting control), CD4 (no CD4 expression
    S2 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of selected RNAi treatments on Nef-induced CD4 downregulation in stably transfected S2 cells. (A) CD4 expression profiles of S2 cells stably expressing CD4 and Nef, after treatment with dsRNAs targeting GFP (nontargeting control), CD4 (no CD4 expression

    Journal:

    Article Title: Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor ▿

    doi: 10.1128/JVI.02725-06

    Figure Lengend Snippet: Effect of selected RNAi treatments on Nef-induced CD4 downregulation in stably transfected S2 cells. (A) CD4 expression profiles of S2 cells stably expressing CD4 and Nef, after treatment with dsRNAs targeting GFP (nontargeting control), CD4 (no CD4 expression

    Article Snippet: One day after transient transfection, the expression of Nef was induced in S2 cells by treatment with 0.5 mM CuSO4 for 24 h. HeLa cells were transiently transfected with the indicated plasmids by using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer.

    Techniques: Stable Transfection, Transfection, Expressing

    Immunofluorescence microscopy of CD4 in S2 cells. Cells were transiently transfected with pAc.CD4 and pMt.NL4-3 Nef (wild type or the indicated mutant), incubated for 24 h, and left untreated (C, E, G, I, and K) or treated with CuSO 4  (D, F, H, J, and

    Journal:

    Article Title: Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor ▿

    doi: 10.1128/JVI.02725-06

    Figure Lengend Snippet: Immunofluorescence microscopy of CD4 in S2 cells. Cells were transiently transfected with pAc.CD4 and pMt.NL4-3 Nef (wild type or the indicated mutant), incubated for 24 h, and left untreated (C, E, G, I, and K) or treated with CuSO 4 (D, F, H, J, and

    Article Snippet: One day after transient transfection, the expression of Nef was induced in S2 cells by treatment with 0.5 mM CuSO4 for 24 h. HeLa cells were transiently transfected with the indicated plasmids by using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer.

    Techniques: Immunofluorescence, Microscopy, Transfection, Mutagenesis, Incubation

    Downregulation of human CD4 by HIV-1 Nef in Drosophila S2 cells. (A) Immunoblot analysis of S2 cells 48 h after transient transfection with pAc.CD4 and pMt.Nef and 24 h after induction of Nef expression with CuSO 4 . Lysates from an untransfected control

    Journal:

    Article Title: Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor ▿

    doi: 10.1128/JVI.02725-06

    Figure Lengend Snippet: Downregulation of human CD4 by HIV-1 Nef in Drosophila S2 cells. (A) Immunoblot analysis of S2 cells 48 h after transient transfection with pAc.CD4 and pMt.Nef and 24 h after induction of Nef expression with CuSO 4 . Lysates from an untransfected control

    Article Snippet: One day after transient transfection, the expression of Nef was induced in S2 cells by treatment with 0.5 mM CuSO4 for 24 h. HeLa cells were transiently transfected with the indicated plasmids by using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer.

    Techniques: Transfection, Expressing

    RNAi screen in S2 cells reveals a requirement for clathrin and AP2 in the Nef-induced downregulation of CD4.

    Journal:

    Article Title: Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor ▿

    doi: 10.1128/JVI.02725-06

    Figure Lengend Snippet: RNAi screen in S2 cells reveals a requirement for clathrin and AP2 in the Nef-induced downregulation of CD4.

    Article Snippet: One day after transient transfection, the expression of Nef was induced in S2 cells by treatment with 0.5 mM CuSO4 for 24 h. HeLa cells were transiently transfected with the indicated plasmids by using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer.

    Techniques:

    Comparison of the downregulation of CD4 by Nef from various HIV-1 and SIV variants in Drosophila S2 and human JM CD4 + T cells. (A) FACS histograms of Drosophila S2 cells that were doubly transfected with pAc.CD4 and pMt vectors encoding a variety

    Journal:

    Article Title: Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor ▿

    doi: 10.1128/JVI.02725-06

    Figure Lengend Snippet: Comparison of the downregulation of CD4 by Nef from various HIV-1 and SIV variants in Drosophila S2 and human JM CD4 + T cells. (A) FACS histograms of Drosophila S2 cells that were doubly transfected with pAc.CD4 and pMt vectors encoding a variety

    Article Snippet: One day after transient transfection, the expression of Nef was induced in S2 cells by treatment with 0.5 mM CuSO4 for 24 h. HeLa cells were transiently transfected with the indicated plasmids by using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer.

    Techniques: FACS, Transfection

    Determinants of Nef-induced CD4 downregulation in Drosophila S2 and human JM CD4 + T cells. (A) FACS histograms of Drosophila S2 cells cotransfected with pAc.CD4 (wild-type or LL413,414AA mutant) and pMt.Nef (wild-type or various mutants). FACS

    Journal:

    Article Title: Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor ▿

    doi: 10.1128/JVI.02725-06

    Figure Lengend Snippet: Determinants of Nef-induced CD4 downregulation in Drosophila S2 and human JM CD4 + T cells. (A) FACS histograms of Drosophila S2 cells cotransfected with pAc.CD4 (wild-type or LL413,414AA mutant) and pMt.Nef (wild-type or various mutants). FACS

    Article Snippet: One day after transient transfection, the expression of Nef was induced in S2 cells by treatment with 0.5 mM CuSO4 for 24 h. HeLa cells were transiently transfected with the indicated plasmids by using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer.

    Techniques: FACS, Mutagenesis

    Results of the RNAi screen of 68 components of the protein trafficking machinery for their involvement in Nef-induced CD4 downregulation in S2 cells. Mean ± the SEM ( n  = 2 to 10) CD4 levels of cells treated with dsRNAs targeting 68 candidate

    Journal:

    Article Title: Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor ▿

    doi: 10.1128/JVI.02725-06

    Figure Lengend Snippet: Results of the RNAi screen of 68 components of the protein trafficking machinery for their involvement in Nef-induced CD4 downregulation in S2 cells. Mean ± the SEM ( n = 2 to 10) CD4 levels of cells treated with dsRNAs targeting 68 candidate

    Article Snippet: One day after transient transfection, the expression of Nef was induced in S2 cells by treatment with 0.5 mM CuSO4 for 24 h. HeLa cells were transiently transfected with the indicated plasmids by using Lipofectamine 2000 (Invitrogen) according to the instructions of the manufacturer.

    Techniques: