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  • 85
    Thermo Fisher hla dr1
    Hla Dr1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hla dr1/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hla dr1 - by Bioz Stars, 2021-09
    85/100 stars
      Buy from Supplier

    86
    Merck & Co hla dr1
    <t>HLA-DR1</t> neonatal monocytes isolated from frozen PBMCs are deficient at MHC-II antigen processing and presentation of protein antigen and presentation of exogenous peptide
    Hla Dr1, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hla dr1/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hla dr1 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Beckman Coulter dr1 ha tetramer
    Frequencies of circulating <t>DR1-HA</t> tetramer-positive cells ex vivo. (A) Example of ex vivo DR1-HA tetramer staining of CD4 T cells before and after magnetic bead enrichment for PE-positive cells. PBMCs were stained with PE-conjugated DR1-HA tetramer, APC-conjugated anti-CD4 antibody, PerCP-conjugated anti-CD14 and -CD19 antibodies to exclude monocytes and B cells, respectively, and Via-Probe to exclude dead cells. Cells were incubated with anti-PE microbeads, and PE-positive cells were selected (enriched) on a magnetic column. Plots are gated on CD4 + CD14 − CD19 − Via-Probe − cells. The plot on the left shows DR1-HA tetramer staining of CD4 + cells before enrichment, and the plot on the right shows the same cell population after enrichment. The frequency of DR1-HA tetramer-positive cells after enrichment was calculated by dividing the number of CD4 + tetramer + cells after enrichment by the input number of CD4 cells. In this example, the input number of CD4 cells was 759,340 and the output number of CD4 + DR1-HA + cells was 46. (B) Reproducibility of the frequency estimations of DR1-HA tetramer-positive cells in six healthy DR1-positive individuals from a single time point. Between two and eight replicate tetramer staining assays were performed on a single PBMC collection from each subject. The frequency of tetramer-positive cells was analyzed as described above. The horizontal bar represents the mean tetramer-positive frequency for each individual. (C) Longitudinal analysis of DR1-HA tetramer-positive cells in two DR1-positive individuals. Four PBMC samples were obtained over a 12-month period and analyzed as described above. The mean frequency of DR1-HA tetramer-positive cells at each time point is plotted for subjects 1 and 4.
    Dr1 Ha Tetramer, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dr1 ha tetramer/product/Beckman Coulter
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dr1 ha tetramer - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    87
    Thermo Fisher gene exp dr1 hs00172424 m1
    Frequencies of circulating <t>DR1-HA</t> tetramer-positive cells ex vivo. (A) Example of ex vivo DR1-HA tetramer staining of CD4 T cells before and after magnetic bead enrichment for PE-positive cells. PBMCs were stained with PE-conjugated DR1-HA tetramer, APC-conjugated anti-CD4 antibody, PerCP-conjugated anti-CD14 and -CD19 antibodies to exclude monocytes and B cells, respectively, and Via-Probe to exclude dead cells. Cells were incubated with anti-PE microbeads, and PE-positive cells were selected (enriched) on a magnetic column. Plots are gated on CD4 + CD14 − CD19 − Via-Probe − cells. The plot on the left shows DR1-HA tetramer staining of CD4 + cells before enrichment, and the plot on the right shows the same cell population after enrichment. The frequency of DR1-HA tetramer-positive cells after enrichment was calculated by dividing the number of CD4 + tetramer + cells after enrichment by the input number of CD4 cells. In this example, the input number of CD4 cells was 759,340 and the output number of CD4 + DR1-HA + cells was 46. (B) Reproducibility of the frequency estimations of DR1-HA tetramer-positive cells in six healthy DR1-positive individuals from a single time point. Between two and eight replicate tetramer staining assays were performed on a single PBMC collection from each subject. The frequency of tetramer-positive cells was analyzed as described above. The horizontal bar represents the mean tetramer-positive frequency for each individual. (C) Longitudinal analysis of DR1-HA tetramer-positive cells in two DR1-positive individuals. Four PBMC samples were obtained over a 12-month period and analyzed as described above. The mean frequency of DR1-HA tetramer-positive cells at each time point is plotted for subjects 1 and 4.
    Gene Exp Dr1 Hs00172424 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp dr1 hs00172424 m1/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp dr1 hs00172424 m1 - by Bioz Stars, 2021-09
    87/100 stars
      Buy from Supplier

    Image Search Results


    HLA-DR1 neonatal monocytes isolated from frozen PBMCs are deficient at MHC-II antigen processing and presentation of protein antigen and presentation of exogenous peptide

    Journal:

    Article Title: Class II MHC antigen presentation defect in neonatal monocytes is not correlated with decreased MHC-II expression

    doi: 10.1016/j.cellimm.2007.01.003

    Figure Lengend Snippet: HLA-DR1 neonatal monocytes isolated from frozen PBMCs are deficient at MHC-II antigen processing and presentation of protein antigen and presentation of exogenous peptide

    Article Snippet: Using our new strategy, antigen processing and presentation function was analyzed in negatively selected monocytes derived from frozen PBMC samples that expressed HLA-DR1 (allele DRB1*0101, ) or HLA-DR3 (allele DRB1*0301, ).

    Techniques: Isolation

    Frequencies of circulating DR1-HA tetramer-positive cells ex vivo. (A) Example of ex vivo DR1-HA tetramer staining of CD4 T cells before and after magnetic bead enrichment for PE-positive cells. PBMCs were stained with PE-conjugated DR1-HA tetramer, APC-conjugated anti-CD4 antibody, PerCP-conjugated anti-CD14 and -CD19 antibodies to exclude monocytes and B cells, respectively, and Via-Probe to exclude dead cells. Cells were incubated with anti-PE microbeads, and PE-positive cells were selected (enriched) on a magnetic column. Plots are gated on CD4 + CD14 − CD19 − Via-Probe − cells. The plot on the left shows DR1-HA tetramer staining of CD4 + cells before enrichment, and the plot on the right shows the same cell population after enrichment. The frequency of DR1-HA tetramer-positive cells after enrichment was calculated by dividing the number of CD4 + tetramer + cells after enrichment by the input number of CD4 cells. In this example, the input number of CD4 cells was 759,340 and the output number of CD4 + DR1-HA + cells was 46. (B) Reproducibility of the frequency estimations of DR1-HA tetramer-positive cells in six healthy DR1-positive individuals from a single time point. Between two and eight replicate tetramer staining assays were performed on a single PBMC collection from each subject. The frequency of tetramer-positive cells was analyzed as described above. The horizontal bar represents the mean tetramer-positive frequency for each individual. (C) Longitudinal analysis of DR1-HA tetramer-positive cells in two DR1-positive individuals. Four PBMC samples were obtained over a 12-month period and analyzed as described above. The mean frequency of DR1-HA tetramer-positive cells at each time point is plotted for subjects 1 and 4.

    Journal: Journal of Virology

    Article Title: Ex Vivo Phenotype and Frequency of Influenza Virus-Specific CD4 Memory T Cells

    doi: 10.1128/JVI.78.13.7284-7287.2004

    Figure Lengend Snippet: Frequencies of circulating DR1-HA tetramer-positive cells ex vivo. (A) Example of ex vivo DR1-HA tetramer staining of CD4 T cells before and after magnetic bead enrichment for PE-positive cells. PBMCs were stained with PE-conjugated DR1-HA tetramer, APC-conjugated anti-CD4 antibody, PerCP-conjugated anti-CD14 and -CD19 antibodies to exclude monocytes and B cells, respectively, and Via-Probe to exclude dead cells. Cells were incubated with anti-PE microbeads, and PE-positive cells were selected (enriched) on a magnetic column. Plots are gated on CD4 + CD14 − CD19 − Via-Probe − cells. The plot on the left shows DR1-HA tetramer staining of CD4 + cells before enrichment, and the plot on the right shows the same cell population after enrichment. The frequency of DR1-HA tetramer-positive cells after enrichment was calculated by dividing the number of CD4 + tetramer + cells after enrichment by the input number of CD4 cells. In this example, the input number of CD4 cells was 759,340 and the output number of CD4 + DR1-HA + cells was 46. (B) Reproducibility of the frequency estimations of DR1-HA tetramer-positive cells in six healthy DR1-positive individuals from a single time point. Between two and eight replicate tetramer staining assays were performed on a single PBMC collection from each subject. The frequency of tetramer-positive cells was analyzed as described above. The horizontal bar represents the mean tetramer-positive frequency for each individual. (C) Longitudinal analysis of DR1-HA tetramer-positive cells in two DR1-positive individuals. Four PBMC samples were obtained over a 12-month period and analyzed as described above. The mean frequency of DR1-HA tetramer-positive cells at each time point is plotted for subjects 1 and 4.

    Article Snippet: All results shown were obtained using the DR1-HA tetramer purchased from Beckman Coulter, with the exception of the results for the first three time points for subjects 1 and 4 shown in Fig. , which were obtained using the DR1-HA tetramer constructed as previously described ( ).

    Techniques: Ex Vivo, Staining, Incubation