Journal: Frontiers in Microbiology
Article Title: Genotypic and Phenotypic Characterization of Novel Sequence Types of Carbapenem-Resistant Acinetobacter baumannii, With Heterogeneous Resistance Determinants and Targeted Variations in Efflux Operons
Figure Lengend Snippet: (A) Linear genome map of A. baumannii strains DR1, DR2, and AB067 with other completely sequenced strains. Different strains used are A. baumannii strain 1461963 (JEWQ01), 1293320 (JFEE01), PR333 (NGDW01), OIFC111 (AMFY01), AB900 (ABXK01), AB900 (JAAVKC01), PR389 (NGEW01), PR373 (NGDA01), ARLG1941 (NGIN01), J9 (CP041587), DR1 (NOXP01), J15 (VKGL01), MRSN31461 (VHFT01), ABBL071 (LLGB01), PM2696 (JAAGSK01), VB25836 (RHLW01), BA18786 (JAAEGP01), AB_026 (RIAH01), AB067 (LZOC01), GML_KP7_Col_R_AB_TR (QHHE01), 4300STDY7045687 (UFIZ01), SGH0905 (PYEH01), 554L (NKXQ01), Aci00835 (VAFQ01), KUFAR39 (NEPK01), 461 (LCTE01), SP2107 (JAAGSY01), DR2 (NOXO01), SP2181 (JAAGTC01), XH731 (CP021321), AB252 (LXNE01), KT722 (RXIN01), GE_2017_62 (WIHB01), abaum000 (UWVS01), ACICU (CP031380), AYE (CU459141), SDF (CU468230), ATCC19606 (CZWC01), and ATCC_17978 (CP000521). Accession numbers are mentioned in parentheses for each genome. (B) Average nucleotide identity (ANI) analysis of A. baumannii strains DR1, DR2, and AB067 with other sequenced strains; values clearly indicate nucleotide level genome similarity in MDR strains used in this study belong to international clone 2. (C) Phylogenomic tree inferred based on single-nucleotide polymorphisms (SNP) in A. baumannii strains using maximum likelihood method. Unrooted phylogenomic tree was analyzed, and strains A. baumannii DR1 belong to ST128 (ST49) group, whereas both A. baumannii strains DR2 and AB067 that belonged to ST1816 (ST195) were distinctly clustered.
Article Snippet: The pmrAB locus was identified on (NOXP01000007.1, NOXO01000017.1, and LZOC01000005.1) in the genomes of strains DR1, DR2, and AB067, respectively, which exhibited > 99% identity with homologs from A. baumannii ATCC 17978.