Journal: British Journal of Cancer

Article Title: Glucocorticoid resistance in T-lineage acute lymphoblastic leukaemia is associated with a proliferative metabolism

doi: 10.1038/sj.bjc.6605072

Figure Lengend Snippet: Top 20 correlating probe sets associated with steroid resistance (IC50 scores) in T-ALL cell lines

Article Snippet: Sensitisation of the relatively DEX-resistant CEM-C1 cell line by rapamycin has been confirmed but the IC50 for these reported CEM variants is quoted as >10 μ M , substantially less than our own (spontaneously resistant) CEM line (>250 μ M ) ( Beesley et al , 2006 ).

Techniques: Migration

Journal: British Journal of Cancer

Article Title: Glucocorticoid resistance in T-lineage acute lymphoblastic leukaemia is associated with a proliferative metabolism

doi: 10.1038/sj.bjc.6605072

Figure Lengend Snippet: Top 10 gene sets from GSEA database C2 (top) and ICHR Curated (bottom) datasets enriched in the MPRED-resistance signature

Article Snippet: Sensitisation of the relatively DEX-resistant CEM-C1 cell line by rapamycin has been confirmed but the IC50 for these reported CEM variants is quoted as >10 μ M , substantially less than our own (spontaneously resistant) CEM line (>250 μ M ) ( Beesley et al , 2006 ).

Techniques: Functional Assay, Expressing

Journal: British Journal of Cancer

Article Title: Glucocorticoid resistance in T-lineage acute lymphoblastic leukaemia is associated with a proliferative metabolism

doi: 10.1038/sj.bjc.6605072

Figure Lengend Snippet: Effect of serum starvation on drug sensitivity in HSB2 cells. Cells were incubated in tissue culture medium containing either 10% or 1% fetal calf serum (FCS) for 7 days before measuring drug sensitivity. ( A ) Growth inhibitory effects of serum starvation after 7 days (arbitrary units, mean±s.e.m., n =9). ( B ) Cytotoxic effects of ARAC, DEX and MPRED (MTT assay, 4-day drug incubation). Data represent the mean percentage change in IC50 in serum-starved cells (1%) compared to control (10%) in 4–7 independent experiments.

Article Snippet: Sensitisation of the relatively DEX-resistant CEM-C1 cell line by rapamycin has been confirmed but the IC50 for these reported CEM variants is quoted as >10 μ M , substantially less than our own (spontaneously resistant) CEM line (>250 μ M ) ( Beesley et al , 2006 ).

Techniques: Incubation, MTT Assay

Journal: British Journal of Cancer

Article Title: Glucocorticoid resistance in T-lineage acute lymphoblastic leukaemia is associated with a proliferative metabolism

doi: 10.1038/sj.bjc.6605072

Figure Lengend Snippet: Prognostic significance of leading edge gene sets associated with MPRED resistance in T-ALL cell lines. Graphs show relapse-free survival in primary ALL patient cohorts (T lineage and B lineage as indicated) predicted based on expression of genes associated with MPRED resistance in T-ALL cell lines. The gene profiles represent the leading edges of the following GSEA gene sets: ( A ) CHOLESTEROL BIOSYNTHESIS; ( B ) STRING GLUL TOP100 ASSOCIATIONS (genes associated with glutamine synthetase); ( C and D ) V$MYC_Q2 (genes regulated by MYC ); ( E and F ) WEI_DOWN_IN_DEX_RESISTANCE (genes associated with DEX resistance); ( G ) VOXPHOS (oxidative phosphorylation); ( H ) PENG_RAPAMYCIN_DOWN (genes downregulated in response to rapamycin). Further details of these gene sets can be found in and .

Article Snippet: Sensitisation of the relatively DEX-resistant CEM-C1 cell line by rapamycin has been confirmed but the IC50 for these reported CEM variants is quoted as >10 μ M , substantially less than our own (spontaneously resistant) CEM line (>250 μ M ) ( Beesley et al , 2006 ).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Glucocorticoid resistance in T-lineage acute lymphoblastic leukaemia is associated with a proliferative metabolism

doi: 10.1038/sj.bjc.6605072

Figure Lengend Snippet: CMAP drugs significantly associated with MPRED- and DEX-resistance gene signatures in T-ALL cell lines

Article Snippet: Sensitisation of the relatively DEX-resistant CEM-C1 cell line by rapamycin has been confirmed but the IC50 for these reported CEM variants is quoted as >10 μ M , substantially less than our own (spontaneously resistant) CEM line (>250 μ M ) ( Beesley et al , 2006 ).

Techniques:

Journal: British Journal of Cancer

Article Title: Glucocorticoid resistance in T-lineage acute lymphoblastic leukaemia is associated with a proliferative metabolism

doi: 10.1038/sj.bjc.6605072

Figure Lengend Snippet: Synergy of CMAP-identified drugs with DEX in T-ALL cell lines. Graphs show cell survival following 48 h incubation. Comparisons were made in each data set to the respective control condition, which was set to 100%. Vehicle control (C) and the following drug treatments were analysed ( A ) 10 n M (R10), 25 n M (R25), 50 n M (R50) rapamycin and 1 μ M DEX (D1) in ALL-SIL; ( B ) 1 μ M (D1), 10 μ M (D10), 500 μ M (D500) DEX and 50 n M rapamycin (R50) in CEM; ( C ) 10 μ M (L10), 20 μ M (L20) LY294002 and 1 μ M DEX (D1) in ALL-SIL; ( D ) 10 μ M (Q10), 25 μ M (Q25) quercetin and 1 μ M DEX (D1) in ALL-SIL; ( E ) 10 μ M (R10), 50 μ M (R50), 100 μ M (R100) resveratrol and 1 μ M DEX (D1) in ALL-SIL; * P <0.05, ** P <0.01 (repeated measures ANOVA). Data represent the mean of 4–5 experiments measured in triplicate (±s.e.m.).

Article Snippet: Sensitisation of the relatively DEX-resistant CEM-C1 cell line by rapamycin has been confirmed but the IC50 for these reported CEM variants is quoted as >10 μ M , substantially less than our own (spontaneously resistant) CEM line (>250 μ M ) ( Beesley et al , 2006 ).

Techniques: Incubation