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  • 94
    Sino Biological sars cov sars cov 2 spike antibody chimeric mab
    <t>SARS-CoV-2</t> production was improved by a temperature reduction to 33 °C but not by the addition of 0.5% TrypLE (experiment 7 and 8). Before the time of infection (dashed vertical line), pH values and concentrations of glucose and lactate were measured before and after adjustment at each time point. After infection, glucose concentrations and pH values were measured before and after each harvest. Harvests were carried out twice per day, and cultures were maintained without (experiment 7, left panels) or with (experiment 8, right panels) 0.5% ( v/v ) TrypLE added to the cell culture medium. The two experiments were done independently. ( A ) Cell culture supernatant glucose concentrations (black dots) and pH values (grey dots); the dashed blue horizontal line represents a glucose concentration of 8.3 mM, the red horizontal line represents a pH value of 7, indicating the desired lower limits for these parameters. ( B ) Cell culture supernatant lactate concentrations. ( C ) Total cell numbers per CelCradle TM 500-AP calculated from a sample of five carriers (black dots) and GCR (grey dots). ( D ) RNA titers of individual harvests (red diamonds, only evaluated for experiment 7), infectivity titers of individual harvests (grey columns, showing the geometric mean of triplicate titrations and error bars indicating standard error of the mean), and SARS-CoV-2 yield expressed as accumulated TCID 50 units (black dots, sum of TCID 50 units obtained from each harvest of an experiment).
    Sars Cov Sars Cov 2 Spike Antibody Chimeric Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov sars cov 2 spike antibody chimeric mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov sars cov 2 spike antibody chimeric mab - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    92
    Gold Biotechnology Inc bp plus dna ladder
    <t>SARS-CoV-2</t> production was improved by a temperature reduction to 33 °C but not by the addition of 0.5% TrypLE (experiment 7 and 8). Before the time of infection (dashed vertical line), pH values and concentrations of glucose and lactate were measured before and after adjustment at each time point. After infection, glucose concentrations and pH values were measured before and after each harvest. Harvests were carried out twice per day, and cultures were maintained without (experiment 7, left panels) or with (experiment 8, right panels) 0.5% ( v/v ) TrypLE added to the cell culture medium. The two experiments were done independently. ( A ) Cell culture supernatant glucose concentrations (black dots) and pH values (grey dots); the dashed blue horizontal line represents a glucose concentration of 8.3 mM, the red horizontal line represents a pH value of 7, indicating the desired lower limits for these parameters. ( B ) Cell culture supernatant lactate concentrations. ( C ) Total cell numbers per CelCradle TM 500-AP calculated from a sample of five carriers (black dots) and GCR (grey dots). ( D ) RNA titers of individual harvests (red diamonds, only evaluated for experiment 7), infectivity titers of individual harvests (grey columns, showing the geometric mean of triplicate titrations and error bars indicating standard error of the mean), and SARS-CoV-2 yield expressed as accumulated TCID 50 units (black dots, sum of TCID 50 units obtained from each harvest of an experiment).
    Bp Plus Dna Ladder, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp plus dna ladder/product/Gold Biotechnology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bp plus dna ladder - by Bioz Stars, 2022-10
    92/100 stars
      Buy from Supplier

    80
    Sino Biological antibody d003
    <t>SARS-CoV-2</t> production was improved by a temperature reduction to 33 °C but not by the addition of 0.5% TrypLE (experiment 7 and 8). Before the time of infection (dashed vertical line), pH values and concentrations of glucose and lactate were measured before and after adjustment at each time point. After infection, glucose concentrations and pH values were measured before and after each harvest. Harvests were carried out twice per day, and cultures were maintained without (experiment 7, left panels) or with (experiment 8, right panels) 0.5% ( v/v ) TrypLE added to the cell culture medium. The two experiments were done independently. ( A ) Cell culture supernatant glucose concentrations (black dots) and pH values (grey dots); the dashed blue horizontal line represents a glucose concentration of 8.3 mM, the red horizontal line represents a pH value of 7, indicating the desired lower limits for these parameters. ( B ) Cell culture supernatant lactate concentrations. ( C ) Total cell numbers per CelCradle TM 500-AP calculated from a sample of five carriers (black dots) and GCR (grey dots). ( D ) RNA titers of individual harvests (red diamonds, only evaluated for experiment 7), infectivity titers of individual harvests (grey columns, showing the geometric mean of triplicate titrations and error bars indicating standard error of the mean), and SARS-CoV-2 yield expressed as accumulated TCID 50 units (black dots, sum of TCID 50 units obtained from each harvest of an experiment).
    Antibody D003, supplied by Sino Biological, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody d003/product/Sino Biological
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody d003 - by Bioz Stars, 2022-10
    80/100 stars
      Buy from Supplier

    Image Search Results


    SARS-CoV-2 production was improved by a temperature reduction to 33 °C but not by the addition of 0.5% TrypLE (experiment 7 and 8). Before the time of infection (dashed vertical line), pH values and concentrations of glucose and lactate were measured before and after adjustment at each time point. After infection, glucose concentrations and pH values were measured before and after each harvest. Harvests were carried out twice per day, and cultures were maintained without (experiment 7, left panels) or with (experiment 8, right panels) 0.5% ( v/v ) TrypLE added to the cell culture medium. The two experiments were done independently. ( A ) Cell culture supernatant glucose concentrations (black dots) and pH values (grey dots); the dashed blue horizontal line represents a glucose concentration of 8.3 mM, the red horizontal line represents a pH value of 7, indicating the desired lower limits for these parameters. ( B ) Cell culture supernatant lactate concentrations. ( C ) Total cell numbers per CelCradle TM 500-AP calculated from a sample of five carriers (black dots) and GCR (grey dots). ( D ) RNA titers of individual harvests (red diamonds, only evaluated for experiment 7), infectivity titers of individual harvests (grey columns, showing the geometric mean of triplicate titrations and error bars indicating standard error of the mean), and SARS-CoV-2 yield expressed as accumulated TCID 50 units (black dots, sum of TCID 50 units obtained from each harvest of an experiment).

    Journal: Vaccines

    Article Title: SARS-CoV-2 Production in a Scalable High Cell Density Bioreactor

    doi: 10.3390/vaccines9070706

    Figure Lengend Snippet: SARS-CoV-2 production was improved by a temperature reduction to 33 °C but not by the addition of 0.5% TrypLE (experiment 7 and 8). Before the time of infection (dashed vertical line), pH values and concentrations of glucose and lactate were measured before and after adjustment at each time point. After infection, glucose concentrations and pH values were measured before and after each harvest. Harvests were carried out twice per day, and cultures were maintained without (experiment 7, left panels) or with (experiment 8, right panels) 0.5% ( v/v ) TrypLE added to the cell culture medium. The two experiments were done independently. ( A ) Cell culture supernatant glucose concentrations (black dots) and pH values (grey dots); the dashed blue horizontal line represents a glucose concentration of 8.3 mM, the red horizontal line represents a pH value of 7, indicating the desired lower limits for these parameters. ( B ) Cell culture supernatant lactate concentrations. ( C ) Total cell numbers per CelCradle TM 500-AP calculated from a sample of five carriers (black dots) and GCR (grey dots). ( D ) RNA titers of individual harvests (red diamonds, only evaluated for experiment 7), infectivity titers of individual harvests (grey columns, showing the geometric mean of triplicate titrations and error bars indicating standard error of the mean), and SARS-CoV-2 yield expressed as accumulated TCID 50 units (black dots, sum of TCID 50 units obtained from each harvest of an experiment).

    Article Snippet: 96-well Maxisorp plates (Nunc) were coated with 2.5 µg/mL capture antibody (Sino Biologicals #40150-D003) diluted in PBS.

    Techniques: Infection, Cell Culture, Concentration Assay

    Higher virus yield obtained in SARS-CoV-2 production at 37 °C with two as compared to one harvest per day (experiments 4, 5 and 6). All culture bottles were seeded with 1.5 × 10 8 cells per bottle. Before the time of infection (dashed vertical line) pH values and concentrations of glucose and lactate were measured before and after adjustment at each time point. After infection, glucose concentrations and pH values were measured before harvest (experiments 4 and 5, left and middle panel) or before and after each harvest (experiment 6, right panel). Harvest frequency is indicated in the figure. The three experiments were done independently. ( A ) Cell culture supernatant glucose concentrations (black dots) and pH values (grey dots). The dashed blue horizontal line represents a glucose concentration of 8.3 mM, the red horizontal line represents a pH value of 7, indicating the desired lower limits for these parameters. ( B ) Cell culture supernatant lactate concentrations. ( C ) Total cell numbers per CelCradle TM 500-AP calculated from a sample of five carriers (black dots) and GCR (grey dots). ( D ) Infectivity titers of individual harvests (grey columns, showing the geometric mean of triplicate titrations and error bars indicating standard error of the mean) and SARS-CoV-2 yield expressed as accumulated 50% tissue culture infectious dose (TCID 50 ) units (black dots, sum of TCID 50 units obtained from each harvest of an experiment).

    Journal: Vaccines

    Article Title: SARS-CoV-2 Production in a Scalable High Cell Density Bioreactor

    doi: 10.3390/vaccines9070706

    Figure Lengend Snippet: Higher virus yield obtained in SARS-CoV-2 production at 37 °C with two as compared to one harvest per day (experiments 4, 5 and 6). All culture bottles were seeded with 1.5 × 10 8 cells per bottle. Before the time of infection (dashed vertical line) pH values and concentrations of glucose and lactate were measured before and after adjustment at each time point. After infection, glucose concentrations and pH values were measured before harvest (experiments 4 and 5, left and middle panel) or before and after each harvest (experiment 6, right panel). Harvest frequency is indicated in the figure. The three experiments were done independently. ( A ) Cell culture supernatant glucose concentrations (black dots) and pH values (grey dots). The dashed blue horizontal line represents a glucose concentration of 8.3 mM, the red horizontal line represents a pH value of 7, indicating the desired lower limits for these parameters. ( B ) Cell culture supernatant lactate concentrations. ( C ) Total cell numbers per CelCradle TM 500-AP calculated from a sample of five carriers (black dots) and GCR (grey dots). ( D ) Infectivity titers of individual harvests (grey columns, showing the geometric mean of triplicate titrations and error bars indicating standard error of the mean) and SARS-CoV-2 yield expressed as accumulated 50% tissue culture infectious dose (TCID 50 ) units (black dots, sum of TCID 50 units obtained from each harvest of an experiment).

    Article Snippet: 96-well Maxisorp plates (Nunc) were coated with 2.5 µg/mL capture antibody (Sino Biologicals #40150-D003) diluted in PBS.

    Techniques: Infection, Cell Culture, Concentration Assay