Article Title: Shared evolutionary origin of vertebrate neural crest and cranial placodes
Figure Lengend Snippet: Sensory cell lineages. a , Cell lineages of anterior blastomeres (a-line blastomeres) and a posterior blastomere (b-line) from the 16-cell to 110-cell stages. Green identifies Dmrt.a -expressing blastomeres, magenta identifies Msxb expression lineages, and yellow identifies Foxc expression. b, Schematic illustration of 16 cell stage embryos. Each of the blastomeres that was labeled with Dil or DiO is indicated by magenta or green. c-e , Head regions of larvae labeled with DiI or DiO at the 16 cell stage. c , Labeling of the a5.3 lineage. d , Labeling of the a5.4 lineage. e, labeling b5.3 blastomere. f-h , Head region of a larva that was co-injected with Dmrt.a > CFP and Msxb > mCherry reporter genes. Arrowhead identifies the boundary of the Dmrt.a/Msxb expression territories. i-k, Head region of a larva co-injected with Foxc > CFP and Dmrt.a > mCherry reporter genes. Anterior to the left; scale bars, 100μm.
Article Snippet: DiI (Celltracker™ CM-DiI Dye, C7000, Molecular probe, Eugene, OR) and DiO (D-275, Molecular probe, Eugene, OR) were dissolved in soybean oil at a concentration of 5mg/ml. (see ).
Techniques: Expressing, Labeling, Injection