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  • 94
    ATCC 8e5 cells
    Specific BiKE-mediated killing. ( a ) Killing of CHO-ZA-gp160 SC cells in presence of NK cells. Target cells were pre-labelled by PHK26 and incubated with serially diluted BiKEs or mD1.22-Fc followed by adding NK cells. The dead cells were measured by PI staining before evaluation by FACS analysis. Experiments were performed in triplicate and the error bars denote ± SD, n = 3. Represents NK cells incubated with mbk6 and CHO-ZA-gp160 SC cells (NK cells + mbk6 + CHO-ZA-gp160 SC , denoted as mbk6 in the figure); , NK cells + mbk11 + CHO-ZA-gp160 SC (denoted as mbk11); , NK cells + mD1.22-Fc + CHO-ZA-gp160 SC (denoted as mD1.22-Fc); other groups are negative controls , in which NK cells incubation with CHO-ZA-gp160 SC cells in the absence of BiKEs, or NK cells incubated gp160 negative CHO-ZA cells in the presence of BiKEs or mD1.22-Fc. , NK cells + mD1.22-Fc + CHO-ZA; , NK cells + CHO-ZA-gp160 SC ; , NK cells + mbk6 + CHO-ZA; , NK cells + mbk11 + CHO-ZA. ( b ) Killing of chronically HIV-1 infected cells <t>(8E5)</t> by human PBMCs mediated by BiKEs. Experiments were performed by co-incubation of PBMCs with BiKE opsonized 8E5 cells and the assay was developed by detecting LDH activity using Promega CytoTox-ONE™ Homogeneous Membrane Integrity kit. The anti-MERS-CoV mAb, IgG1 m336 was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2.
    8e5 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8e5 cells/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8e5 cells - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    86
    ATCC intracellular hiv
    Specific BiKE-mediated killing. ( a ) Killing of CHO-ZA-gp160 SC cells in presence of NK cells. Target cells were pre-labelled by PHK26 and incubated with serially diluted BiKEs or mD1.22-Fc followed by adding NK cells. The dead cells were measured by PI staining before evaluation by FACS analysis. Experiments were performed in triplicate and the error bars denote ± SD, n = 3. Represents NK cells incubated with mbk6 and CHO-ZA-gp160 SC cells (NK cells + mbk6 + CHO-ZA-gp160 SC , denoted as mbk6 in the figure); , NK cells + mbk11 + CHO-ZA-gp160 SC (denoted as mbk11); , NK cells + mD1.22-Fc + CHO-ZA-gp160 SC (denoted as mD1.22-Fc); other groups are negative controls , in which NK cells incubation with CHO-ZA-gp160 SC cells in the absence of BiKEs, or NK cells incubated gp160 negative CHO-ZA cells in the presence of BiKEs or mD1.22-Fc. , NK cells + mD1.22-Fc + CHO-ZA; , NK cells + CHO-ZA-gp160 SC ; , NK cells + mbk6 + CHO-ZA; , NK cells + mbk11 + CHO-ZA. ( b ) Killing of chronically HIV-1 infected cells <t>(8E5)</t> by human PBMCs mediated by BiKEs. Experiments were performed by co-incubation of PBMCs with BiKE opsonized 8E5 cells and the assay was developed by detecting LDH activity using Promega CytoTox-ONE™ Homogeneous Membrane Integrity kit. The anti-MERS-CoV mAb, IgG1 m336 was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2.
    Intracellular Hiv, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intracellular hiv/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    intracellular hiv - by Bioz Stars, 2023-01
    86/100 stars
      Buy from Supplier

    Image Search Results


    Specific BiKE-mediated killing. ( a ) Killing of CHO-ZA-gp160 SC cells in presence of NK cells. Target cells were pre-labelled by PHK26 and incubated with serially diluted BiKEs or mD1.22-Fc followed by adding NK cells. The dead cells were measured by PI staining before evaluation by FACS analysis. Experiments were performed in triplicate and the error bars denote ± SD, n = 3. Represents NK cells incubated with mbk6 and CHO-ZA-gp160 SC cells (NK cells + mbk6 + CHO-ZA-gp160 SC , denoted as mbk6 in the figure); , NK cells + mbk11 + CHO-ZA-gp160 SC (denoted as mbk11); , NK cells + mD1.22-Fc + CHO-ZA-gp160 SC (denoted as mD1.22-Fc); other groups are negative controls , in which NK cells incubation with CHO-ZA-gp160 SC cells in the absence of BiKEs, or NK cells incubated gp160 negative CHO-ZA cells in the presence of BiKEs or mD1.22-Fc. , NK cells + mD1.22-Fc + CHO-ZA; , NK cells + CHO-ZA-gp160 SC ; , NK cells + mbk6 + CHO-ZA; , NK cells + mbk11 + CHO-ZA. ( b ) Killing of chronically HIV-1 infected cells (8E5) by human PBMCs mediated by BiKEs. Experiments were performed by co-incubation of PBMCs with BiKE opsonized 8E5 cells and the assay was developed by detecting LDH activity using Promega CytoTox-ONE™ Homogeneous Membrane Integrity kit. The anti-MERS-CoV mAb, IgG1 m336 was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2.

    Journal: Scientific Reports

    Article Title: One-domain CD4 Fused to Human Anti-CD16 Antibody Domain Mediates Effective Killing of HIV-1-Infected Cells

    doi: 10.1038/s41598-017-07966-3

    Figure Lengend Snippet: Specific BiKE-mediated killing. ( a ) Killing of CHO-ZA-gp160 SC cells in presence of NK cells. Target cells were pre-labelled by PHK26 and incubated with serially diluted BiKEs or mD1.22-Fc followed by adding NK cells. The dead cells were measured by PI staining before evaluation by FACS analysis. Experiments were performed in triplicate and the error bars denote ± SD, n = 3. Represents NK cells incubated with mbk6 and CHO-ZA-gp160 SC cells (NK cells + mbk6 + CHO-ZA-gp160 SC , denoted as mbk6 in the figure); , NK cells + mbk11 + CHO-ZA-gp160 SC (denoted as mbk11); , NK cells + mD1.22-Fc + CHO-ZA-gp160 SC (denoted as mD1.22-Fc); other groups are negative controls , in which NK cells incubation with CHO-ZA-gp160 SC cells in the absence of BiKEs, or NK cells incubated gp160 negative CHO-ZA cells in the presence of BiKEs or mD1.22-Fc. , NK cells + mD1.22-Fc + CHO-ZA; , NK cells + CHO-ZA-gp160 SC ; , NK cells + mbk6 + CHO-ZA; , NK cells + mbk11 + CHO-ZA. ( b ) Killing of chronically HIV-1 infected cells (8E5) by human PBMCs mediated by BiKEs. Experiments were performed by co-incubation of PBMCs with BiKE opsonized 8E5 cells and the assay was developed by detecting LDH activity using Promega CytoTox-ONE™ Homogeneous Membrane Integrity kit. The anti-MERS-CoV mAb, IgG1 m336 was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2.

    Article Snippet: 8E5 cells (ATCC) were plated on round-bottom 96-well plates at a density of 2.5 × 10 4 cells in 25 μL RPMI1640 complete medium containing 20 IU/mL hIL-2 per well.

    Techniques: Incubation, Staining, Infection, Activity Assay, Negative Control