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  • 99
    ATCC l wnt3a cells
    Luciferase activity in CHO cells transfected with WT-LRP5 or mutant LRP5 in the presence of <t>Wnt3a-CM</t> (white bars) or L1 control medium (grey bars) . The activities of the signaling pathway are presented in relative luciferase units (RLU) determined by the ratio between the luciferase and β-galactosidase activities and given as a fold change relative to corresponding samples treated with 10% FBS-DMEM. * p < 0.05, ** p < 0.01 as compared with WT-LRP5. Results are expressed as mean ± SD.
    L Wnt3a Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l wnt3a cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l wnt3a cells - by Bioz Stars, 2024-05
    99/100 stars
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    99
    ATCC control l cells
    a) Electron microscopy of Ex‐WNT3a and Ex‐C. Includes an overview and zoomed in images. Scale bars on all images represent 0.2 μm. b i and ii) Nanoparticle tracking analysis of representative Ex‐WNT3a and Ex‐C preparations using NanosightTM(NS300). b iii) Comparison of number of particles and modal size of the particles (nm) for both Ex‐C and Ex‐ENT3a across different batches. c) Western blot for WNT3a and Tsg101 (exosomal marker) of 20 ul of supernatant (SNo) and exosome pellet/fraction (Ex fract) of L‐WNT3a and control L‐cells, following 100,000 × g ultracentrifugation (n = 2). d) Western blot for WNT3a using a standard curve of R‐WNT3a [Rec] and varying amounts of Ex‐WNT3a [Ex] to determine concentration of WNT3a in exosome preparation (n = 3). Detailed explanation in supplementary Figure
    Control L Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control l cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control l cells - by Bioz Stars, 2024-05
    99/100 stars
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    86
    LGC Promochem crl 2647 wnt3aexpressing mouse l
    a) Electron microscopy of Ex‐WNT3a and Ex‐C. Includes an overview and zoomed in images. Scale bars on all images represent 0.2 μm. b i and ii) Nanoparticle tracking analysis of representative Ex‐WNT3a and Ex‐C preparations using NanosightTM(NS300). b iii) Comparison of number of particles and modal size of the particles (nm) for both Ex‐C and Ex‐ENT3a across different batches. c) Western blot for WNT3a and Tsg101 (exosomal marker) of 20 ul of supernatant (SNo) and exosome pellet/fraction (Ex fract) of L‐WNT3a and control L‐cells, following 100,000 × g ultracentrifugation (n = 2). d) Western blot for WNT3a using a standard curve of R‐WNT3a [Rec] and varying amounts of Ex‐WNT3a [Ex] to determine concentration of WNT3a in exosome preparation (n = 3). Detailed explanation in supplementary Figure
    Crl 2647 Wnt3aexpressing Mouse L, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crl 2647 wnt3aexpressing mouse l/product/LGC Promochem
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    crl 2647 wnt3aexpressing mouse l - by Bioz Stars, 2024-05
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    Image Search Results


    Luciferase activity in CHO cells transfected with WT-LRP5 or mutant LRP5 in the presence of Wnt3a-CM (white bars) or L1 control medium (grey bars) . The activities of the signaling pathway are presented in relative luciferase units (RLU) determined by the ratio between the luciferase and β-galactosidase activities and given as a fold change relative to corresponding samples treated with 10% FBS-DMEM. * p < 0.05, ** p < 0.01 as compared with WT-LRP5. Results are expressed as mean ± SD.

    Journal: BMC Medical Genetics

    Article Title: Mutations in LRP5 cause primary osteoporosis without features of OI by reducing Wnt signaling activity

    doi: 10.1186/1471-2350-13-26

    Figure Lengend Snippet: Luciferase activity in CHO cells transfected with WT-LRP5 or mutant LRP5 in the presence of Wnt3a-CM (white bars) or L1 control medium (grey bars) . The activities of the signaling pathway are presented in relative luciferase units (RLU) determined by the ratio between the luciferase and β-galactosidase activities and given as a fold change relative to corresponding samples treated with 10% FBS-DMEM. * p < 0.05, ** p < 0.01 as compared with WT-LRP5. Results are expressed as mean ± SD.

    Article Snippet: The control-conditioned medium (L1-CM) was prepared from a normal L1 cell line (ATCC CRL-2648) using the same protocol as for the L Wnt3a cells.

    Techniques: Luciferase, Activity Assay, Transfection, Mutagenesis

    A) Tph1 gene expression and B) 5- Htr1b gene expression in CHO cells transfected with wild type LRP5 (WT-LRP5) or mutant LRP5 and treated with Wnt3a-CM or L1-CM . Expression of the Tph1 and 5- Htr1b genes is shown as a fold change relative to the untreated control (pcDNA3.1+) and normalized to β-actin . The effects of the LRP5 mutants on the expression of these genes were examined by comparing the results for the mutants with those for WT-LRP5 using Student's t test. The statistical significance of each pair is denoted below the mutant columns; ** p < 0.01 as compared to WT-LRP5. Results are expressed as mean ± SD.

    Journal: BMC Medical Genetics

    Article Title: Mutations in LRP5 cause primary osteoporosis without features of OI by reducing Wnt signaling activity

    doi: 10.1186/1471-2350-13-26

    Figure Lengend Snippet: A) Tph1 gene expression and B) 5- Htr1b gene expression in CHO cells transfected with wild type LRP5 (WT-LRP5) or mutant LRP5 and treated with Wnt3a-CM or L1-CM . Expression of the Tph1 and 5- Htr1b genes is shown as a fold change relative to the untreated control (pcDNA3.1+) and normalized to β-actin . The effects of the LRP5 mutants on the expression of these genes were examined by comparing the results for the mutants with those for WT-LRP5 using Student's t test. The statistical significance of each pair is denoted below the mutant columns; ** p < 0.01 as compared to WT-LRP5. Results are expressed as mean ± SD.

    Article Snippet: The control-conditioned medium (L1-CM) was prepared from a normal L1 cell line (ATCC CRL-2648) using the same protocol as for the L Wnt3a cells.

    Techniques: Expressing, Transfection, Mutagenesis

    a) Electron microscopy of Ex‐WNT3a and Ex‐C. Includes an overview and zoomed in images. Scale bars on all images represent 0.2 μm. b i and ii) Nanoparticle tracking analysis of representative Ex‐WNT3a and Ex‐C preparations using NanosightTM(NS300). b iii) Comparison of number of particles and modal size of the particles (nm) for both Ex‐C and Ex‐ENT3a across different batches. c) Western blot for WNT3a and Tsg101 (exosomal marker) of 20 ul of supernatant (SNo) and exosome pellet/fraction (Ex fract) of L‐WNT3a and control L‐cells, following 100,000 × g ultracentrifugation (n = 2). d) Western blot for WNT3a using a standard curve of R‐WNT3a [Rec] and varying amounts of Ex‐WNT3a [Ex] to determine concentration of WNT3a in exosome preparation (n = 3). Detailed explanation in supplementary Figure

    Journal: Journal of Extracellular Vesicles

    Article Title: WNT3A‐loaded exosomes enable cartilage repair

    doi: 10.1002/jev2.12088

    Figure Lengend Snippet: a) Electron microscopy of Ex‐WNT3a and Ex‐C. Includes an overview and zoomed in images. Scale bars on all images represent 0.2 μm. b i and ii) Nanoparticle tracking analysis of representative Ex‐WNT3a and Ex‐C preparations using NanosightTM(NS300). b iii) Comparison of number of particles and modal size of the particles (nm) for both Ex‐C and Ex‐ENT3a across different batches. c) Western blot for WNT3a and Tsg101 (exosomal marker) of 20 ul of supernatant (SNo) and exosome pellet/fraction (Ex fract) of L‐WNT3a and control L‐cells, following 100,000 × g ultracentrifugation (n = 2). d) Western blot for WNT3a using a standard curve of R‐WNT3a [Rec] and varying amounts of Ex‐WNT3a [Ex] to determine concentration of WNT3a in exosome preparation (n = 3). Detailed explanation in supplementary Figure

    Article Snippet: Conditioned medium was generated using serum‐free medium containing ITS supplement (Sigma I3146), as per the manufacturer's instructions from L‐cells stably transfected with WNT3a and control L‐cells (ATCC CRL2647/ CRL2648).

    Techniques: Electron Microscopy, Western Blot, Marker, Concentration Assay