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  • ls174  (ATCC)
    99
    ATCC ls174
    Enhanced expression of MMP-13, TLR-9 and second messengers of the TLR signal transduction cascade in CRC cells. (A) <t>LS174</t> cells (black bars) showed an enhanced gene transcription of MMP-13 (84-fold, p=0.016) and TLR-9 (75-fold, p=0.016), and also of MyD88
    Ls174, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore mmp13 cl 82198
    Enhanced expression of MMP-13, TLR-9 and second messengers of the TLR signal transduction cascade in CRC cells. (A) <t>LS174</t> cells (black bars) showed an enhanced gene transcription of MMP-13 (84-fold, p=0.016) and TLR-9 (75-fold, p=0.016), and also of MyD88
    Mmp13 Cl 82198, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Selleck Chemicals cl amidine
    Enhanced expression of MMP-13, TLR-9 and second messengers of the TLR signal transduction cascade in CRC cells. (A) <t>LS174</t> cells (black bars) showed an enhanced gene transcription of MMP-13 (84-fold, p=0.016) and TLR-9 (75-fold, p=0.016), and also of MyD88
    Cl Amidine, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Nikon cl quant software
    Enhanced expression of MMP-13, TLR-9 and second messengers of the TLR signal transduction cascade in CRC cells. (A) <t>LS174</t> cells (black bars) showed an enhanced gene transcription of MMP-13 (84-fold, p=0.016) and TLR-9 (75-fold, p=0.016), and also of MyD88
    Cl Quant Software, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ls180  (ATCC)
    95
    ATCC ls180
    Knockdown of hPXR expression abolished MET-induced CYP3A4 and MDR1 expression in <t>LS180-hPXR-CYP3A4-luc</t> cells Human (A) CYP3A4 and (B) MDR1 mRNA expression levels were determined in LS180-hPXR-CYP3A4-luc cells transiently transfected with hPXR siRNA (siPXR) or non-target control siRNA (NTsiRNA) before 48-h treatments with the indicated compounds. (* p
    Ls180, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wrl68  (ATCC)
    93
    ATCC wrl68
    Cytotoxic effects of HAB16R13 against PC-12 and <t>WRL68</t> cell lines . Each point is expressed as the mean of three independent experiments.
    Wrl68, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Tocris cl 4as 1
    In vitro analysis of AR expression and treatment in RCC cell lines ( A ) Representative images of AR expression in two different RCC cell lines (ACHN: upper panel, Caki2: lower panel) as determined by IHC. ( B ) Percentage of negative (0+), weakly positive (1+), moderately positive (2+) and strongly positive (3+) cells. ( C ) MTT absorbance (cell viability) of ACHN and Caki2 cells after treatment with <t>Cl-4AS-1,</t> a steroidal androgen receptor agonist. ( D ) Cresylviolett (KRV) assay absorbance of ACHN and Caki2 cells after treatment with Cl-4AS-1, a steroidal androgen receptor agonist. [AU] = arbitrary unit.
    Cl 4as 1, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Tocris cl hibo
    (A) Binding of [ 3 <t>H]AMPA</t> to the S1S2 domains of GluR2 o and GluR3 i . The K D reported a K D of 24.8 ± 1.8 nM for [ 3 H]AMPA binding to GluR2. (B) Structures of the ligands used in the binding studies, (C) The inhibition of [ 3 H]AMPA binding by agonists, partial agonists, and antagonist to the S1S2 domains of GluR2 o and GluR3 i . Except for willardiine, the IC 50 values were within 2-fold for the two subtypes: (ligand, GluR2 IC 50 /GluR3 IC 50 ; IC 50 expressed in μM) fluorowillardiine, 0.0040±.0009/0.0062±0.0014; iodowillardiine, 0.46±0.05/0.79±0.14; <t>Cl-HIBO,</t> 5.0±0.3/55±4; willardiine, 3.1±0.2/0.99±0.18; UBP277, 135±12/69±10. In all cases, GluR2 o is shown with filled symbols, and GluR3 i is shown with open symbols.
    Cl Hibo, supplied by Tocris, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore cl 316 243 hydrate cl
    COX-2 activity is required for the ADRB3-stimulated CCL2 up-regulation in animal adipose tissues.  C57BL/6 mice (male, 8-week-old) were  i.p.  injected with celecoxib (100 mg/kg) or control vehicle for 1 h. Subsequently, mice were  i.p.  injected with or without CL 316–243 (10 nmol). Three hours later, EWAT were collected, and measured for levels of COX-2 ( A ), COX-1 ( B ), CCL2/MCP-1 ( D ), and IL-6 ( E ) by qPCR analysis. **,  p
    Cl 316 243 Hydrate Cl, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Cayman Chemical cl amidine cl a
    COX-2 activity is required for the ADRB3-stimulated CCL2 up-regulation in animal adipose tissues.  C57BL/6 mice (male, 8-week-old) were  i.p.  injected with celecoxib (100 mg/kg) or control vehicle for 1 h. Subsequently, mice were  i.p.  injected with or without CL 316–243 (10 nmol). Three hours later, EWAT were collected, and measured for levels of COX-2 ( A ), COX-1 ( B ), CCL2/MCP-1 ( D ), and IL-6 ( E ) by qPCR analysis. **,  p
    Cl Amidine Cl A, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Enhanced expression of MMP-13, TLR-9 and second messengers of the TLR signal transduction cascade in CRC cells. (A) LS174 cells (black bars) showed an enhanced gene transcription of MMP-13 (84-fold, p=0.016) and TLR-9 (75-fold, p=0.016), and also of MyD88

    Journal: Oncology Letters

    Article Title: Matrix metalloproteinase-13 is regulated by toll-like receptor-9 in colorectal cancer cells and mediates cellular migration

    doi: 10.3892/ol.2011.276

    Figure Lengend Snippet: Enhanced expression of MMP-13, TLR-9 and second messengers of the TLR signal transduction cascade in CRC cells. (A) LS174 cells (black bars) showed an enhanced gene transcription of MMP-13 (84-fold, p=0.016) and TLR-9 (75-fold, p=0.016), and also of MyD88

    Article Snippet: Human colon cancer cell lines SW620 (ATCC, CCL-227) and LS174 (ATCC, CL-188) were employed for this study.

    Techniques: Expressing, Transduction

    Specific inhibition of MMP-13 significantly reduces the migration of LS174 cells. LS174 cells were incubated in the presence of 10 and 20 μM CL-82198 or 10 μM BSA. Migration was assessed after 24 h of incubation with the respective substances.

    Journal: Oncology Letters

    Article Title: Matrix metalloproteinase-13 is regulated by toll-like receptor-9 in colorectal cancer cells and mediates cellular migration

    doi: 10.3892/ol.2011.276

    Figure Lengend Snippet: Specific inhibition of MMP-13 significantly reduces the migration of LS174 cells. LS174 cells were incubated in the presence of 10 and 20 μM CL-82198 or 10 μM BSA. Migration was assessed after 24 h of incubation with the respective substances.

    Article Snippet: Human colon cancer cell lines SW620 (ATCC, CCL-227) and LS174 (ATCC, CL-188) were employed for this study.

    Techniques: Inhibition, Migration, Incubation

    Effect of alisertib and TAK-733 alone and in combination on p53, p73, and p21 . Evaluation was performed in p53 mutant (DLD-1) and wild type (LS174T) CRC cell lines at 24 and 72 h, with a more robust increase in p53 observed at both 24 and 72 h in the p53 mutant DLD-1 cell line. An increase in p21 is demonstrated in the DLD-1 cells exposed to alisertib alone, with no change noted in the LS174T cell line.

    Journal: Frontiers in Pharmacology

    Article Title: Combined inhibition of MEK and Aurora A kinase in KRAS/PIK3CA double-mutant colorectal cancer models

    doi: 10.3389/fphar.2015.00120

    Figure Lengend Snippet: Effect of alisertib and TAK-733 alone and in combination on p53, p73, and p21 . Evaluation was performed in p53 mutant (DLD-1) and wild type (LS174T) CRC cell lines at 24 and 72 h, with a more robust increase in p53 observed at both 24 and 72 h in the p53 mutant DLD-1 cell line. An increase in p21 is demonstrated in the DLD-1 cells exposed to alisertib alone, with no change noted in the LS174T cell line.

    Article Snippet: Cell lines and culture The human colorectal cancer cell lines HCT116, DLD-1, LS174T, LS180, HCT-15, SW948, T84, Mip101, were obtained from American Type Culture Collection and SNU1544 was obtained from the Korean Cell Line Bank, and identities were confirmed by DNA profiling at the University of Colorado Cancer Center DNA Sequencing and Analysis Core.

    Techniques: Mutagenesis

    Trichostatin A-induced modulation of PMCA expression in various gastric/colon cancer cell lines: time course. KATO-III ( A ), DLD-1 ( B ) and LS-174T ( C ) cells were treated with the indicated concentrations of trichostatin A for 4 days (KATO-III and DLD-1 cells) or for 3 days (LS-174T cells). Total cellular lysates were prepared at the indicated time points and equal amounts of cellular proteins (from 20 to 30 μg/lane, depending on cell types and the antibody used for immunostaining) were analyzed for overall PMCA (5F10), PMCA4b (JA3) and Na + /K + -ATPase expressions. The time course of the trichostatin A-induced up-regulation of PMCA4b expression reached a plateau-phase at days 1-2 for KATO-III and LS-174T cells and at days 2-3 for DLD-1 cells.

    Journal: Cell calcium

    Article Title: Isoform-Specific Up-Regulation of Plasma Membrane Ca2+ATPase Expression During Colon and Gastric Cancer Cell Differentiation

    doi: 10.1016/j.ceca.2007.02.003

    Figure Lengend Snippet: Trichostatin A-induced modulation of PMCA expression in various gastric/colon cancer cell lines: time course. KATO-III ( A ), DLD-1 ( B ) and LS-174T ( C ) cells were treated with the indicated concentrations of trichostatin A for 4 days (KATO-III and DLD-1 cells) or for 3 days (LS-174T cells). Total cellular lysates were prepared at the indicated time points and equal amounts of cellular proteins (from 20 to 30 μg/lane, depending on cell types and the antibody used for immunostaining) were analyzed for overall PMCA (5F10), PMCA4b (JA3) and Na + /K + -ATPase expressions. The time course of the trichostatin A-induced up-regulation of PMCA4b expression reached a plateau-phase at days 1-2 for KATO-III and LS-174T cells and at days 2-3 for DLD-1 cells.

    Article Snippet: The epithelial HeLa, the kidney fibroblast-like COS-7, the gastric carcinoma KATO-III, as well as the DLD-1, HT-29, LS-174T and Caco-2 colon carcinoma cell lines were obtained from the ATCC (Manassas, VA, USA).

    Techniques: Expressing, Immunostaining

    Expression of PMCA-type Ca 2+ pumps in gastric and colon cancer cell lines. Equal amounts (30 μg/lane) of total cellular proteins from various gastric (KATO-III) and colon (DLD-1, Caco-2, LS-174T and HT-29) cancer cell lines were loaded for SDS-PAGE, electroblotted and immunostained for: overall PMCA expression using the 5F10 ( upper panel ); PMCA4b expression using the JA3 ( middle panel ) and for PMCA4 (4a and 4b) expression using the JA9 ( lower panel ) monoclonal antibodies. 5 μg of microsomal membrane protein from the epithelial HeLa cells was mixed with 25 ng of microsomal membrane protein from COS-7 cells transfected with the PMCA4a cDNA. This mixed sample was loaded onto the gel to mark the position of the PMCA1b, 4a and 4b isoforms within a single sample. PMCA1b is the major PMCA isoform in the gastric/colon cancer cell lines. The expression of PMCA4b, but not that of the PMCA4a protein could also be detected in these cells at a lower level.

    Journal: Cell calcium

    Article Title: Isoform-Specific Up-Regulation of Plasma Membrane Ca2+ATPase Expression During Colon and Gastric Cancer Cell Differentiation

    doi: 10.1016/j.ceca.2007.02.003

    Figure Lengend Snippet: Expression of PMCA-type Ca 2+ pumps in gastric and colon cancer cell lines. Equal amounts (30 μg/lane) of total cellular proteins from various gastric (KATO-III) and colon (DLD-1, Caco-2, LS-174T and HT-29) cancer cell lines were loaded for SDS-PAGE, electroblotted and immunostained for: overall PMCA expression using the 5F10 ( upper panel ); PMCA4b expression using the JA3 ( middle panel ) and for PMCA4 (4a and 4b) expression using the JA9 ( lower panel ) monoclonal antibodies. 5 μg of microsomal membrane protein from the epithelial HeLa cells was mixed with 25 ng of microsomal membrane protein from COS-7 cells transfected with the PMCA4a cDNA. This mixed sample was loaded onto the gel to mark the position of the PMCA1b, 4a and 4b isoforms within a single sample. PMCA1b is the major PMCA isoform in the gastric/colon cancer cell lines. The expression of PMCA4b, but not that of the PMCA4a protein could also be detected in these cells at a lower level.

    Article Snippet: The epithelial HeLa, the kidney fibroblast-like COS-7, the gastric carcinoma KATO-III, as well as the DLD-1, HT-29, LS-174T and Caco-2 colon carcinoma cell lines were obtained from the ATCC (Manassas, VA, USA).

    Techniques: Expressing, SDS Page, Transfection

    SCFA-induced modulation of PMCA expression in various gastric/colon cancer cell lines: concentration dependence. KATO-III ( A ), DLD-1 ( B ), HT-29 ( C ) and LS-174T ( D ) cells were cultured in the presence of various concentrations of Na + -valerate or Na + -butyrate, as indicated on the panels . Total cellular protein lysates were prepared from all treatments at day 3 for KATO-III, DLD-1 and HT-29, and at day 2 for LS-174T cells. Equal amounts of cellular proteins (from 20 to 50 μg/lane, depending on the cell lines and the antibody used for immunostaining) were loaded onto the SDS-polyacrylamide gels, electroblotted and immunostained for overall PMCA (5F10) and PMCA4b (JA3) expressions. The expression of the ubiquitous SERCA2 pump, and that of various established differentiation markers (CEA and/or SERCA3) were monitored in parallel. Treatment of the four cancer cell lines with these differentiation-inducing SCFAs led to the marked up-regulation of PMCA4b expression and to a less pronounced induction of PMCA1b expression.

    Journal: Cell calcium

    Article Title: Isoform-Specific Up-Regulation of Plasma Membrane Ca2+ATPase Expression During Colon and Gastric Cancer Cell Differentiation

    doi: 10.1016/j.ceca.2007.02.003

    Figure Lengend Snippet: SCFA-induced modulation of PMCA expression in various gastric/colon cancer cell lines: concentration dependence. KATO-III ( A ), DLD-1 ( B ), HT-29 ( C ) and LS-174T ( D ) cells were cultured in the presence of various concentrations of Na + -valerate or Na + -butyrate, as indicated on the panels . Total cellular protein lysates were prepared from all treatments at day 3 for KATO-III, DLD-1 and HT-29, and at day 2 for LS-174T cells. Equal amounts of cellular proteins (from 20 to 50 μg/lane, depending on the cell lines and the antibody used for immunostaining) were loaded onto the SDS-polyacrylamide gels, electroblotted and immunostained for overall PMCA (5F10) and PMCA4b (JA3) expressions. The expression of the ubiquitous SERCA2 pump, and that of various established differentiation markers (CEA and/or SERCA3) were monitored in parallel. Treatment of the four cancer cell lines with these differentiation-inducing SCFAs led to the marked up-regulation of PMCA4b expression and to a less pronounced induction of PMCA1b expression.

    Article Snippet: The epithelial HeLa, the kidney fibroblast-like COS-7, the gastric carcinoma KATO-III, as well as the DLD-1, HT-29, LS-174T and Caco-2 colon carcinoma cell lines were obtained from the ATCC (Manassas, VA, USA).

    Techniques: Expressing, Concentration Assay, Cell Culture, Immunostaining

    Trichostatin A-induced modulation of PMCA expression in various gastric/colon cancer cell lines: concentration dependence. KATO-III ( A ), DLD-1 ( B ) and LS-174T ( C ) cells were treated with various concentrations of trichostatin A or 3 mM Na + -butyrate as indicated on the panels . Total cellular lysates were prepared from all treatments at day 3 for KATO-III, at day 4 for DLD-1 and at day 2 for LS-174T cells. Equal amounts of cellular proteins (from 20 to 30 μg/lane, depending on cell types and the antibody used for immunostaining) were analyzed for overall PMCA (5F10) and PMCA4b (JA3) expressions. Treatment of the three cancer cell lines with the HDAC inhibitor trichostatin A led to a marked up-regulation of PMCA4b expression.

    Journal: Cell calcium

    Article Title: Isoform-Specific Up-Regulation of Plasma Membrane Ca2+ATPase Expression During Colon and Gastric Cancer Cell Differentiation

    doi: 10.1016/j.ceca.2007.02.003

    Figure Lengend Snippet: Trichostatin A-induced modulation of PMCA expression in various gastric/colon cancer cell lines: concentration dependence. KATO-III ( A ), DLD-1 ( B ) and LS-174T ( C ) cells were treated with various concentrations of trichostatin A or 3 mM Na + -butyrate as indicated on the panels . Total cellular lysates were prepared from all treatments at day 3 for KATO-III, at day 4 for DLD-1 and at day 2 for LS-174T cells. Equal amounts of cellular proteins (from 20 to 30 μg/lane, depending on cell types and the antibody used for immunostaining) were analyzed for overall PMCA (5F10) and PMCA4b (JA3) expressions. Treatment of the three cancer cell lines with the HDAC inhibitor trichostatin A led to a marked up-regulation of PMCA4b expression.

    Article Snippet: The epithelial HeLa, the kidney fibroblast-like COS-7, the gastric carcinoma KATO-III, as well as the DLD-1, HT-29, LS-174T and Caco-2 colon carcinoma cell lines were obtained from the ATCC (Manassas, VA, USA).

    Techniques: Expressing, Concentration Assay, Immunostaining

    Downregulation of miR-214-induced radioresistance of CRC. a Differentially expressed microRNAs in SW837 cells in response to IR treatment by microRNA microarray. U6 was used as internal control. b MiR-214 expression in HT29 and LS174.T CRC cell lines by quantitative real-time PCR analysis. Data represent mean ± SD. * p

    Journal: Oncogenesis

    Article Title: Inhibition of ATG12-mediated autophagy by miR-214 enhances radiosensitivity in colorectal cancer

    doi: 10.1038/s41389-018-0028-8

    Figure Lengend Snippet: Downregulation of miR-214-induced radioresistance of CRC. a Differentially expressed microRNAs in SW837 cells in response to IR treatment by microRNA microarray. U6 was used as internal control. b MiR-214 expression in HT29 and LS174.T CRC cell lines by quantitative real-time PCR analysis. Data represent mean ± SD. * p

    Article Snippet: Human CRC cell lines SW837, LoVo, SW620, SW480, HCT116, HT29, LS174.T, and human embryonal kidney 293 cells were purchased from American Type Culture Collection.

    Techniques: Microarray, Expressing, Real-time Polymerase Chain Reaction

    IR-induced downregulation of miR-214 was associated with increased autophagic activity in CRC cells. a , d Autophagy evaluation of SW480 and HCT116 ( a ) or Ls174.T and HT29 ( d ) cells by transmission electron microscopy (TEM). Arrow head indicated autophagosome-like vesicles induced by IR stimulation in CRC cells. Scale bar, 100 nm. The data were quantified by counting the number of autophagosome-like vesicles per cross-sectioned cell. The data are represented as mean ± SD of values obtained in three independent experiments. ** p

    Journal: Oncogenesis

    Article Title: Inhibition of ATG12-mediated autophagy by miR-214 enhances radiosensitivity in colorectal cancer

    doi: 10.1038/s41389-018-0028-8

    Figure Lengend Snippet: IR-induced downregulation of miR-214 was associated with increased autophagic activity in CRC cells. a , d Autophagy evaluation of SW480 and HCT116 ( a ) or Ls174.T and HT29 ( d ) cells by transmission electron microscopy (TEM). Arrow head indicated autophagosome-like vesicles induced by IR stimulation in CRC cells. Scale bar, 100 nm. The data were quantified by counting the number of autophagosome-like vesicles per cross-sectioned cell. The data are represented as mean ± SD of values obtained in three independent experiments. ** p

    Article Snippet: Human CRC cell lines SW837, LoVo, SW620, SW480, HCT116, HT29, LS174.T, and human embryonal kidney 293 cells were purchased from American Type Culture Collection.

    Techniques: Activity Assay, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy

    Knockdown of hPXR expression abolished MET-induced CYP3A4 and MDR1 expression in LS180-hPXR-CYP3A4-luc cells Human (A) CYP3A4 and (B) MDR1 mRNA expression levels were determined in LS180-hPXR-CYP3A4-luc cells transiently transfected with hPXR siRNA (siPXR) or non-target control siRNA (NTsiRNA) before 48-h treatments with the indicated compounds. (* p

    Journal: Biochemical pharmacology

    Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

    doi: 10.1016/j.bcp.2014.08.025

    Figure Lengend Snippet: Knockdown of hPXR expression abolished MET-induced CYP3A4 and MDR1 expression in LS180-hPXR-CYP3A4-luc cells Human (A) CYP3A4 and (B) MDR1 mRNA expression levels were determined in LS180-hPXR-CYP3A4-luc cells transiently transfected with hPXR siRNA (siPXR) or non-target control siRNA (NTsiRNA) before 48-h treatments with the indicated compounds. (* p

    Article Snippet: HepG2, LS180, and LS174T cells were maintained in modified Eagle’s minimal essential medium (ATCC) with 10% FBS, 2 mM L-glutamine, penicillin (100 units/mL), and streptomycin 100 μg/ml) at 37 °C in a humidified atmosphere containing 5% CO2 .

    Techniques: Expressing, Transfection

    MET activates the PXR-regulated CYP3A4 promoter (A) HepG2, (B) LS180, and (C) LS174T cells transiently transfected with hPXR, CYP3A4-luc, and CMV-Renilla were treated for 24 h with indicated concentrations of rifampicin or MET prior to luciferase assay.

    Journal: Biochemical pharmacology

    Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

    doi: 10.1016/j.bcp.2014.08.025

    Figure Lengend Snippet: MET activates the PXR-regulated CYP3A4 promoter (A) HepG2, (B) LS180, and (C) LS174T cells transiently transfected with hPXR, CYP3A4-luc, and CMV-Renilla were treated for 24 h with indicated concentrations of rifampicin or MET prior to luciferase assay.

    Article Snippet: HepG2, LS180, and LS174T cells were maintained in modified Eagle’s minimal essential medium (ATCC) with 10% FBS, 2 mM L-glutamine, penicillin (100 units/mL), and streptomycin 100 μg/ml) at 37 °C in a humidified atmosphere containing 5% CO2 .

    Techniques: Transfection, Luciferase

    Cytotoxicity of MET in (A) HepG2, (B) LS180, and (C) LS 174T cells Cells were treated with increasing concentrations (1.7 nM to 56 μM) of MET.

    Journal: Biochemical pharmacology

    Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

    doi: 10.1016/j.bcp.2014.08.025

    Figure Lengend Snippet: Cytotoxicity of MET in (A) HepG2, (B) LS180, and (C) LS 174T cells Cells were treated with increasing concentrations (1.7 nM to 56 μM) of MET.

    Article Snippet: HepG2, LS180, and LS174T cells were maintained in modified Eagle’s minimal essential medium (ATCC) with 10% FBS, 2 mM L-glutamine, penicillin (100 units/mL), and streptomycin 100 μg/ml) at 37 °C in a humidified atmosphere containing 5% CO2 .

    Techniques:

    MET induced mRNA and protein expression of CYP3A4 and MDR1 in LS180 and LS174T intestinal cells Real-time PCR analysis of (A) CYP3A4 and (B) MDR1 mRNA expression in LS180 cells and (C) CYP3A4 and (D) MDR1 mRNA expression in LS174T cells after 48-h treatment with different compounds as indicated (* p

    Journal: Biochemical pharmacology

    Article Title: Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1

    doi: 10.1016/j.bcp.2014.08.025

    Figure Lengend Snippet: MET induced mRNA and protein expression of CYP3A4 and MDR1 in LS180 and LS174T intestinal cells Real-time PCR analysis of (A) CYP3A4 and (B) MDR1 mRNA expression in LS180 cells and (C) CYP3A4 and (D) MDR1 mRNA expression in LS174T cells after 48-h treatment with different compounds as indicated (* p

    Article Snippet: HepG2, LS180, and LS174T cells were maintained in modified Eagle’s minimal essential medium (ATCC) with 10% FBS, 2 mM L-glutamine, penicillin (100 units/mL), and streptomycin 100 μg/ml) at 37 °C in a humidified atmosphere containing 5% CO2 .

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Specificity analysis of ND-1-conjugated QD probes. Notes: ( A ) Immunofluorescence analysis of the QD probes specificity. LEA-expressing CL187 cells were labeled with ND-1-biotin and QD605-SA sequentially, and IgG-biotin and PBS were used as controls. ( B ) Specific competitive assay. Before incubation with ND-1-biotin and QD605-SA, CL187 cells were treated with increasing concentrations of nonbiotinylated ND-1 (6.25, 12.5, 25.0, and 50.0 μg/mL) for competitive binding. The fluorescence images were taken (upper row) and changes in intensity were quantified with LSM510 software (Carl Zeiss Meditec AG, Jena, Germany; bottom row). (Magnification, ×400.) Abbreviations: LEA, large external antigen; QD, quantum dot; QD605-SA, quantum dot-conjugated streptavidin with a 605 nm emission wavelength; DAPI, 4,6-diamidino-2-phenylindole dihydrochloride; PBS, phosphate-buffered saline.

    Journal: International Journal of Nanomedicine

    Article Title: Quantitative detection of the tumor-associated antigen large external antigen in colorectal cancer tissues and cells using quantum dot probe

    doi: 10.2147/IJN.S97509

    Figure Lengend Snippet: Specificity analysis of ND-1-conjugated QD probes. Notes: ( A ) Immunofluorescence analysis of the QD probes specificity. LEA-expressing CL187 cells were labeled with ND-1-biotin and QD605-SA sequentially, and IgG-biotin and PBS were used as controls. ( B ) Specific competitive assay. Before incubation with ND-1-biotin and QD605-SA, CL187 cells were treated with increasing concentrations of nonbiotinylated ND-1 (6.25, 12.5, 25.0, and 50.0 μg/mL) for competitive binding. The fluorescence images were taken (upper row) and changes in intensity were quantified with LSM510 software (Carl Zeiss Meditec AG, Jena, Germany; bottom row). (Magnification, ×400.) Abbreviations: LEA, large external antigen; QD, quantum dot; QD605-SA, quantum dot-conjugated streptavidin with a 605 nm emission wavelength; DAPI, 4,6-diamidino-2-phenylindole dihydrochloride; PBS, phosphate-buffered saline.

    Article Snippet: The CL187 and HT29 cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Immunofluorescence, Expressing, Labeling, Incubation, Binding Assay, Fluorescence, Software

    QD-ICC combined with imaging quantitative analysis of LEA expression in various cell lines. Notes: ( A ) QD-ICC images taken by fluorescence microscopy. Colo205, CL187, HT29, and HeLa cells were separately incubated with ND-1-biotin followed by QD605-SA. The nuclei were stained with DAPI. (Magnification, ×400.) ( B ) Quantitative results based on QD-ICC. Five images of each cell type were taken and the fluorescence intensities were quantified. Data were presented as the mean ± standard deviation. ** P

    Journal: International Journal of Nanomedicine

    Article Title: Quantitative detection of the tumor-associated antigen large external antigen in colorectal cancer tissues and cells using quantum dot probe

    doi: 10.2147/IJN.S97509

    Figure Lengend Snippet: QD-ICC combined with imaging quantitative analysis of LEA expression in various cell lines. Notes: ( A ) QD-ICC images taken by fluorescence microscopy. Colo205, CL187, HT29, and HeLa cells were separately incubated with ND-1-biotin followed by QD605-SA. The nuclei were stained with DAPI. (Magnification, ×400.) ( B ) Quantitative results based on QD-ICC. Five images of each cell type were taken and the fluorescence intensities were quantified. Data were presented as the mean ± standard deviation. ** P

    Article Snippet: The CL187 and HT29 cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Immunocytochemistry, Imaging, Expressing, Fluorescence, Microscopy, Incubation, Staining, Standard Deviation

    Purity analysis and immunological activity assay of purified ND-1. Notes: ( A ) ND-1 was purified from mouse ascites fluids and subjected to SDS-PAGE analysis. ( B ) Immunological activity of purified ND-1 was assayed by immunofluorescence technology. CL187 cells were first incubated with ND-1, followed by FITC-labeled goat antimouse IgG. The nuclei were stained with DAPI. (Magnification, ×600.) Abbreviations: MW, molecular weight; kD, kilodalton; FITC, fluorescein isothiocyanate; DAPI, 4,6-diamidino-2-phenylindole dihydrochloride; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Journal: International Journal of Nanomedicine

    Article Title: Quantitative detection of the tumor-associated antigen large external antigen in colorectal cancer tissues and cells using quantum dot probe

    doi: 10.2147/IJN.S97509

    Figure Lengend Snippet: Purity analysis and immunological activity assay of purified ND-1. Notes: ( A ) ND-1 was purified from mouse ascites fluids and subjected to SDS-PAGE analysis. ( B ) Immunological activity of purified ND-1 was assayed by immunofluorescence technology. CL187 cells were first incubated with ND-1, followed by FITC-labeled goat antimouse IgG. The nuclei were stained with DAPI. (Magnification, ×600.) Abbreviations: MW, molecular weight; kD, kilodalton; FITC, fluorescein isothiocyanate; DAPI, 4,6-diamidino-2-phenylindole dihydrochloride; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Article Snippet: The CL187 and HT29 cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Activity Assay, Purification, SDS Page, Immunofluorescence, Incubation, Labeling, Staining, Molecular Weight, Polyacrylamide Gel Electrophoresis

    Cytotoxic effects of HAB16R13 against PC-12 and WRL68 cell lines . Each point is expressed as the mean of three independent experiments.

    Journal: BMC Complementary and Alternative Medicine

    Article Title: BACE1 inhibitory activity of fungal endophytic extracts from Malaysian medicinal plants

    doi: 10.1186/1472-6882-11-79

    Figure Lengend Snippet: Cytotoxic effects of HAB16R13 against PC-12 and WRL68 cell lines . Each point is expressed as the mean of three independent experiments.

    Article Snippet: The extract showing the greatest BACE1 inhibitory activity HAB16R13 was then tested (at various concentrations from 0.1 μg/ml - 1000 μg/ml) for cytotoxicity against PC-12 and WRL68 using the MTT assay [ ].

    Techniques:

    Effects of AA on WRL-68 viability. Notes: WRL-68 cells were treated with AA for 24 hours (gray bars) and 72 hours (dotted bars) at indicated concentrations. Viability was measured using MTT assay and data are the average of four independent experiments (± SD) and were analyzed using one-way ANOVA with Tukey’s post hoc test. *** P

    Journal: Drug Design, Development and Therapy

    Article Title: Immunomodulatory effects of 17-O-acetylacuminolide in RAW264.7 cells and HUVECs: involvement of MAPK and NF-κB pathways

    doi: 10.2147/DDDT.S68659

    Figure Lengend Snippet: Effects of AA on WRL-68 viability. Notes: WRL-68 cells were treated with AA for 24 hours (gray bars) and 72 hours (dotted bars) at indicated concentrations. Viability was measured using MTT assay and data are the average of four independent experiments (± SD) and were analyzed using one-way ANOVA with Tukey’s post hoc test. *** P

    Article Snippet: Cell lines and reagents Cell lines RAW264.7 and WRL-68 were obtained from American Type Culture Collection (ATCC), Manassas, VA, USA.

    Techniques: MTT Assay

    In vitro analysis of AR expression and treatment in RCC cell lines ( A ) Representative images of AR expression in two different RCC cell lines (ACHN: upper panel, Caki2: lower panel) as determined by IHC. ( B ) Percentage of negative (0+), weakly positive (1+), moderately positive (2+) and strongly positive (3+) cells. ( C ) MTT absorbance (cell viability) of ACHN and Caki2 cells after treatment with Cl-4AS-1, a steroidal androgen receptor agonist. ( D ) Cresylviolett (KRV) assay absorbance of ACHN and Caki2 cells after treatment with Cl-4AS-1, a steroidal androgen receptor agonist. [AU] = arbitrary unit.

    Journal: Oncotarget

    Article Title: Prognostic relevance of androgen receptor expression in renal cell carcinomas

    doi: 10.18632/oncotarget.20827

    Figure Lengend Snippet: In vitro analysis of AR expression and treatment in RCC cell lines ( A ) Representative images of AR expression in two different RCC cell lines (ACHN: upper panel, Caki2: lower panel) as determined by IHC. ( B ) Percentage of negative (0+), weakly positive (1+), moderately positive (2+) and strongly positive (3+) cells. ( C ) MTT absorbance (cell viability) of ACHN and Caki2 cells after treatment with Cl-4AS-1, a steroidal androgen receptor agonist. ( D ) Cresylviolett (KRV) assay absorbance of ACHN and Caki2 cells after treatment with Cl-4AS-1, a steroidal androgen receptor agonist. [AU] = arbitrary unit.

    Article Snippet: 5 µM of Cl-4AS-1, a steroidal androgen receptor agonist (Tocris, Bristol, UK) were used to treat the RCC cell lines for 48 h with subsequent cell viability measurements.

    Techniques: In Vitro, Expressing, Immunohistochemistry, MTT Assay

    (A) Binding of [ 3 H]AMPA to the S1S2 domains of GluR2 o and GluR3 i . The K D reported a K D of 24.8 ± 1.8 nM for [ 3 H]AMPA binding to GluR2. (B) Structures of the ligands used in the binding studies, (C) The inhibition of [ 3 H]AMPA binding by agonists, partial agonists, and antagonist to the S1S2 domains of GluR2 o and GluR3 i . Except for willardiine, the IC 50 values were within 2-fold for the two subtypes: (ligand, GluR2 IC 50 /GluR3 IC 50 ; IC 50 expressed in μM) fluorowillardiine, 0.0040±.0009/0.0062±0.0014; iodowillardiine, 0.46±0.05/0.79±0.14; Cl-HIBO, 5.0±0.3/55±4; willardiine, 3.1±0.2/0.99±0.18; UBP277, 135±12/69±10. In all cases, GluR2 o is shown with filled symbols, and GluR3 i is shown with open symbols.

    Journal: Proteins

    Article Title: Structure of the S1S2 Glutamate Binding Domain of GluR3

    doi: 10.1002/prot.22274

    Figure Lengend Snippet: (A) Binding of [ 3 H]AMPA to the S1S2 domains of GluR2 o and GluR3 i . The K D reported a K D of 24.8 ± 1.8 nM for [ 3 H]AMPA binding to GluR2. (B) Structures of the ligands used in the binding studies, (C) The inhibition of [ 3 H]AMPA binding by agonists, partial agonists, and antagonist to the S1S2 domains of GluR2 o and GluR3 i . Except for willardiine, the IC 50 values were within 2-fold for the two subtypes: (ligand, GluR2 IC 50 /GluR3 IC 50 ; IC 50 expressed in μM) fluorowillardiine, 0.0040±.0009/0.0062±0.0014; iodowillardiine, 0.46±0.05/0.79±0.14; Cl-HIBO, 5.0±0.3/55±4; willardiine, 3.1±0.2/0.99±0.18; UBP277, 135±12/69±10. In all cases, GluR2 o is shown with filled symbols, and GluR3 i is shown with open symbols.

    Article Snippet: AMPA and Cl-HIBO were purchased from Tocris (Ellisville, MO).

    Techniques: Binding Assay, Inhibition

    COX-2 activity is required for the ADRB3-stimulated CCL2 up-regulation in animal adipose tissues.  C57BL/6 mice (male, 8-week-old) were  i.p.  injected with celecoxib (100 mg/kg) or control vehicle for 1 h. Subsequently, mice were  i.p.  injected with or without CL 316–243 (10 nmol). Three hours later, EWAT were collected, and measured for levels of COX-2 ( A ), COX-1 ( B ), CCL2/MCP-1 ( D ), and IL-6 ( E ) by qPCR analysis. **,  p

    Journal: The Journal of Biological Chemistry

    Article Title: Characterization of Eicosanoids Produced by Adipocyte Lipolysis

    doi: 10.1074/jbc.M116.725937

    Figure Lengend Snippet: COX-2 activity is required for the ADRB3-stimulated CCL2 up-regulation in animal adipose tissues. C57BL/6 mice (male, 8-week-old) were i.p. injected with celecoxib (100 mg/kg) or control vehicle for 1 h. Subsequently, mice were i.p. injected with or without CL 316–243 (10 nmol). Three hours later, EWAT were collected, and measured for levels of COX-2 ( A ), COX-1 ( B ), CCL2/MCP-1 ( D ), and IL-6 ( E ) by qPCR analysis. **, p

    Article Snippet: CL 316-243 (CL) (Sigma) was dissolved in H2 O. Cyclooxygenase-2 inhibitor, celecoxib (Sigma), was dissolved in DMSO.

    Techniques: Activity Assay, Mouse Assay, Injection, Real-time Polymerase Chain Reaction

    COX-2 activity is required for the ADRB3-stimulated macrophage/monocyte infiltration in adipose tissues.  C57BL/6 mice (male, 8-week-old) were  i.p.  injected with celecoxib (100 mg/kg) or control vehicle for 1 h. Subsequently, mice were  i.p.  injected with or without CL 316–243 (10 nmol). Three hours later, EWAT were collected.  A , paraffin sections (5 μm) of EWAT were immunohistochemically stained with anti-F4/80.  a , vehicle;  b , CL 316-243;  c , celecoxib alone;  d , celecoxib + CL 316–243;  e  and  f , enlarged image of box area in  a  and  b , respectively.  Arrows , infiltrated macrophages/monocytes.  B , macrophages/monocytes present in each treatment (4–5 microscopic fields) were scored. *,  p

    Journal: The Journal of Biological Chemistry

    Article Title: Characterization of Eicosanoids Produced by Adipocyte Lipolysis

    doi: 10.1074/jbc.M116.725937

    Figure Lengend Snippet: COX-2 activity is required for the ADRB3-stimulated macrophage/monocyte infiltration in adipose tissues. C57BL/6 mice (male, 8-week-old) were i.p. injected with celecoxib (100 mg/kg) or control vehicle for 1 h. Subsequently, mice were i.p. injected with or without CL 316–243 (10 nmol). Three hours later, EWAT were collected. A , paraffin sections (5 μm) of EWAT were immunohistochemically stained with anti-F4/80. a , vehicle; b , CL 316-243; c , celecoxib alone; d , celecoxib + CL 316–243; e and f , enlarged image of box area in a and b , respectively. Arrows , infiltrated macrophages/monocytes. B , macrophages/monocytes present in each treatment (4–5 microscopic fields) were scored. *, p

    Article Snippet: CL 316-243 (CL) (Sigma) was dissolved in H2 O. Cyclooxygenase-2 inhibitor, celecoxib (Sigma), was dissolved in DMSO.

    Techniques: Activity Assay, Mouse Assay, Injection, Staining