Journal: Internal Medicine
Article Title: An Autopsy Case of Myeloperoxidase-anti-neutrophil Cytoplasmic Antibody (MPO-ANCA)-associated Vasculitis Accompanied by Cryoglobulinemic Vasculitis Affecting the Kidneys, Skin, and Gastrointestinal Tract
Figure Lengend Snippet: The immunofluorescence findings of NETs in the kidney and skin purpura. The other lesions of glomerulitis (A-F) and cutaneous small arteries of the skin purpura (G-L). (A, G) Hematoxylin and Eosin staining; (B, H) Cit-H3 staining with anti-Cit-H3 and Alexa Fluor 594-conjugated goat anti-rabbit IgG H L (red); (C, I) MPO staining with FITC-conjugated anti-MPO (green); (D, J) DNA staining with DAPI (blue). Cit-H3 and extracellular DNA were co-localized with MPO in the glomeruli (F), but not in the cutaneous arteries (L). The small arteries of the gastric and duodenal ulcers were negative for Cit-H3.
Article Snippet: We used immunofluorescence to detect citrullinated histone 3 (Cit-H3) using anti-Cit-H3 (Abcam, ab5103) and goat anti-rabbit IgG H & L (Abcam, ab150080), DNA stained with 4'6-diamidino-2-phenylindole (DAPI), and MPO stained with anti-MPO (Gene Tex, GTX11729) to visualize NET formation in the skin biopsy and autopsy specimens ( ).
Techniques: Immunofluorescence, Staining