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  • 86
    Abcam cit h3
    Neutrophil extracellular traps (NETs) in mouse kidney following I/R injury a ) Representative confocal images. Top panel: intact neutrophils in kidney sections of mice following sham surgery. Bottom panel: NETs formation in kidney sections of mice following 28-min ischemia and 24-h reperfusion. NETs are defined by co-localization of NE (green) and diffuse DAPI staining patterns. b ) Immunoblot analysis of <t>Cit-H3</t> expression. c ) Quantification of NETs in kidney sections from WT and PAD4 −/− mice following sham surgery or I/R injury (n = 3 – 4).
    Cit H3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Malvern Panalytical cit agnps
    Neutrophil extracellular traps (NETs) in mouse kidney following I/R injury a ) Representative confocal images. Top panel: intact neutrophils in kidney sections of mice following sham surgery. Bottom panel: NETs formation in kidney sections of mice following 28-min ischemia and 24-h reperfusion. NETs are defined by co-localization of NE (green) and diffuse DAPI staining patterns. b ) Immunoblot analysis of <t>Cit-H3</t> expression. c ) Quantification of NETs in kidney sections from WT and PAD4 −/− mice following sham surgery or I/R injury (n = 3 – 4).
    Cit Agnps, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cit agnps/product/Malvern Panalytical
    Average 86 stars, based on 1 article reviews
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    86
    GE Healthcare 123 i fp cit spect
    Neutrophil extracellular traps (NETs) in mouse kidney following I/R injury a ) Representative confocal images. Top panel: intact neutrophils in kidney sections of mice following sham surgery. Bottom panel: NETs formation in kidney sections of mice following 28-min ischemia and 24-h reperfusion. NETs are defined by co-localization of NE (green) and diffuse DAPI staining patterns. b ) Immunoblot analysis of <t>Cit-H3</t> expression. c ) Quantification of NETs in kidney sections from WT and PAD4 −/− mice following sham surgery or I/R injury (n = 3 – 4).
    123 I Fp Cit Spect, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/123 i fp cit spect/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    123 i fp cit spect - by Bioz Stars, 2021-05
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    99
    Abcam anti cit h3
    The immunofluorescence findings of NETs in the kidney and skin purpura. The other lesions of glomerulitis (A-F) and cutaneous small arteries of the skin purpura (G-L). (A, G) Hematoxylin and Eosin staining; (B, H) <t>Cit-H3</t> staining with anti-Cit-H3 and Alexa Fluor 594-conjugated goat anti-rabbit IgG H L (red); (C, I) MPO staining with FITC-conjugated anti-MPO (green); (D, J) DNA staining with DAPI (blue). Cit-H3 and extracellular DNA were co-localized with MPO in the glomeruli (F), but not in the cutaneous arteries (L). The small arteries of the gastric and duodenal ulcers were negative for Cit-H3.
    Anti Cit H3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cit h3/product/Abcam
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    N/A
    L BCME uncharged analog of the trypsin substrate L BAME is cleaved by human urinary arginine esterase 2 and trypsin
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    N/A
    citron rho interacting serine threonine kinase Recombinant Protein Epitope Signature Tag PrEST antigen sequence
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    Image Search Results


    Neutrophil extracellular traps (NETs) in mouse kidney following I/R injury a ) Representative confocal images. Top panel: intact neutrophils in kidney sections of mice following sham surgery. Bottom panel: NETs formation in kidney sections of mice following 28-min ischemia and 24-h reperfusion. NETs are defined by co-localization of NE (green) and diffuse DAPI staining patterns. b ) Immunoblot analysis of Cit-H3 expression. c ) Quantification of NETs in kidney sections from WT and PAD4 −/− mice following sham surgery or I/R injury (n = 3 – 4).

    Journal: Kidney international

    Article Title: Neutrophil peptidyl arginine deiminase-4 has a pivotal role in ischemia/reperfusion-induced acute kidney injury

    doi: 10.1016/j.kint.2017.08.014

    Figure Lengend Snippet: Neutrophil extracellular traps (NETs) in mouse kidney following I/R injury a ) Representative confocal images. Top panel: intact neutrophils in kidney sections of mice following sham surgery. Bottom panel: NETs formation in kidney sections of mice following 28-min ischemia and 24-h reperfusion. NETs are defined by co-localization of NE (green) and diffuse DAPI staining patterns. b ) Immunoblot analysis of Cit-H3 expression. c ) Quantification of NETs in kidney sections from WT and PAD4 −/− mice following sham surgery or I/R injury (n = 3 – 4).

    Article Snippet: Sections were blocked (60 min) in phosphate buffered saline (PBS) supplemented with 10% goat serum and 5% bovine serum albumin, incubated with antibodies against PAD4, Cit-H3, Ly-6G, and neutrophil elastase (NE; Abcam) for 16 h (4°C).

    Techniques: Mouse Assay, Staining, Expressing

    Expression of kidney PAD4 and citrullinated histone-H3 (Cit-H3) after ischemia and reperfusion (I/R) injury a ) qPCR analysis of PAD4 mRNA level in mouse kidneys 24 h after renal I/R injury compared to sham operations (n = 4/group). b ) Immunoblot analysis of Cit-H3 expression in wild-type (WT) mouse kidneys 24 h after I/R injury versus sham-operated control mice; actin was a loading control. Bottom panel: densitometric analysis of Cit-H3 band intensity (n = 3/group). c ) and d ) Immunofluorescence analysis of kidney sections from WT and PAD4 −/− (PAD4KO) mice following sham surgery (Sham) or after 28 min ischemia and 24 h reperfusion. c ) PAD4 (green); Ly-6G (red); DAPI (blue). d ) Cit-H3 (green); Ly-6G (red); DAPI (blue). Immunofluorescence signals double positive for PAD4 and Ly-6G (PAD4 + Ly6G + ), or Cit-H3 and Ly-6G (Cit-H3 + Ly-6G + ) were determined by averaging signal counts from 7 – 10 fields (20× objective) per kidney section per mouse. Data shown are mean ± SEM (n = 3/group). Data were compared one-way ANOVA using a significance level of p

    Journal: Kidney international

    Article Title: Neutrophil peptidyl arginine deiminase-4 has a pivotal role in ischemia/reperfusion-induced acute kidney injury

    doi: 10.1016/j.kint.2017.08.014

    Figure Lengend Snippet: Expression of kidney PAD4 and citrullinated histone-H3 (Cit-H3) after ischemia and reperfusion (I/R) injury a ) qPCR analysis of PAD4 mRNA level in mouse kidneys 24 h after renal I/R injury compared to sham operations (n = 4/group). b ) Immunoblot analysis of Cit-H3 expression in wild-type (WT) mouse kidneys 24 h after I/R injury versus sham-operated control mice; actin was a loading control. Bottom panel: densitometric analysis of Cit-H3 band intensity (n = 3/group). c ) and d ) Immunofluorescence analysis of kidney sections from WT and PAD4 −/− (PAD4KO) mice following sham surgery (Sham) or after 28 min ischemia and 24 h reperfusion. c ) PAD4 (green); Ly-6G (red); DAPI (blue). d ) Cit-H3 (green); Ly-6G (red); DAPI (blue). Immunofluorescence signals double positive for PAD4 and Ly-6G (PAD4 + Ly6G + ), or Cit-H3 and Ly-6G (Cit-H3 + Ly-6G + ) were determined by averaging signal counts from 7 – 10 fields (20× objective) per kidney section per mouse. Data shown are mean ± SEM (n = 3/group). Data were compared one-way ANOVA using a significance level of p

    Article Snippet: Sections were blocked (60 min) in phosphate buffered saline (PBS) supplemented with 10% goat serum and 5% bovine serum albumin, incubated with antibodies against PAD4, Cit-H3, Ly-6G, and neutrophil elastase (NE; Abcam) for 16 h (4°C).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, Immunofluorescence

    The immunofluorescence findings of NETs in the kidney and skin purpura. The other lesions of glomerulitis (A-F) and cutaneous small arteries of the skin purpura (G-L). (A, G) Hematoxylin and Eosin staining; (B, H) Cit-H3 staining with anti-Cit-H3 and Alexa Fluor 594-conjugated goat anti-rabbit IgG H L (red); (C, I) MPO staining with FITC-conjugated anti-MPO (green); (D, J) DNA staining with DAPI (blue). Cit-H3 and extracellular DNA were co-localized with MPO in the glomeruli (F), but not in the cutaneous arteries (L). The small arteries of the gastric and duodenal ulcers were negative for Cit-H3.

    Journal: Internal Medicine

    Article Title: An Autopsy Case of Myeloperoxidase-anti-neutrophil Cytoplasmic Antibody (MPO-ANCA)-associated Vasculitis Accompanied by Cryoglobulinemic Vasculitis Affecting the Kidneys, Skin, and Gastrointestinal Tract

    doi: 10.2169/internalmedicine.0720-17

    Figure Lengend Snippet: The immunofluorescence findings of NETs in the kidney and skin purpura. The other lesions of glomerulitis (A-F) and cutaneous small arteries of the skin purpura (G-L). (A, G) Hematoxylin and Eosin staining; (B, H) Cit-H3 staining with anti-Cit-H3 and Alexa Fluor 594-conjugated goat anti-rabbit IgG H L (red); (C, I) MPO staining with FITC-conjugated anti-MPO (green); (D, J) DNA staining with DAPI (blue). Cit-H3 and extracellular DNA were co-localized with MPO in the glomeruli (F), but not in the cutaneous arteries (L). The small arteries of the gastric and duodenal ulcers were negative for Cit-H3.

    Article Snippet: We used immunofluorescence to detect citrullinated histone 3 (Cit-H3) using anti-Cit-H3 (Abcam, ab5103) and goat anti-rabbit IgG H & L (Abcam, ab150080), DNA stained with 4'6-diamidino-2-phenylindole (DAPI), and MPO stained with anti-MPO (Gene Tex, GTX11729) to visualize NET formation in the skin biopsy and autopsy specimens ( ).

    Techniques: Immunofluorescence, Staining