CIT Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore l citrulline cit
    L Citrulline Cit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l citrulline cit/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l citrulline cit - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    89
    Abcam cit h3
    Immunohistochemical observation of NETs and fibrin markers in thick fibrils in inflammatory exudates in legionnaire’s pneumonia ( a–f ) and liver abscess ( g–l ). HE ( a, g ), <t>Cit-H3</t> ( b, h ), LF ( c, i ), MPO ( d, j ), NE ( e, k ) and FGG ( f, l ). Thick fibrils in legionnaire’s pneumonia show immunoreactivities of all the NETs markers and FGG ( b–f ). Thick fibrils in liver abscess are immunoreactive only for FGG ( l ). Neutrophils intermingled within the meshwork are labeled for the neutrophil markers. Dotted circles represent HE-stained thick fibrils. Bar=50 μm.
    Cit H3, supplied by Abcam, used in various techniques. Bioz Stars score: 89/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cit h3/product/Abcam
    Average 89 stars, based on 132 article reviews
    Price from $9.99 to $1999.99
    cit h3 - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

    91
    Malvern Panalytical cit agnps
    Immunohistochemical observation of NETs and fibrin markers in thick fibrils in inflammatory exudates in legionnaire’s pneumonia ( a–f ) and liver abscess ( g–l ). HE ( a, g ), <t>Cit-H3</t> ( b, h ), LF ( c, i ), MPO ( d, j ), NE ( e, k ) and FGG ( f, l ). Thick fibrils in legionnaire’s pneumonia show immunoreactivities of all the NETs markers and FGG ( b–f ). Thick fibrils in liver abscess are immunoreactive only for FGG ( l ). Neutrophils intermingled within the meshwork are labeled for the neutrophil markers. Dotted circles represent HE-stained thick fibrils. Bar=50 μm.
    Cit Agnps, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cit agnps/product/Malvern Panalytical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cit agnps - by Bioz Stars, 2020-11
    91/100 stars
      Buy from Supplier

    99
    Abcam anti cit h3
    The immunofluorescence findings of NETs in the kidney and skin purpura. The other lesions of glomerulitis (A-F) and cutaneous small arteries of the skin purpura (G-L). (A, G) Hematoxylin and Eosin staining; (B, H) <t>Cit-H3</t> staining with anti-Cit-H3 and Alexa Fluor 594-conjugated goat anti-rabbit IgG H L (red); (C, I) MPO staining with FITC-conjugated anti-MPO (green); (D, J) DNA staining with DAPI (blue). Cit-H3 and extracellular DNA were co-localized with MPO in the glomeruli (F), but not in the cutaneous arteries (L). The small arteries of the gastric and duodenal ulcers were negative for Cit-H3.
    Anti Cit H3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cit h3/product/Abcam
    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    anti cit h3 - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    92
    Levena Biopharma azide peg4 val cit pab monomethyl auristatin e
    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to α-amanitin and monomethyl <t>auristatin</t> E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of <t>maleimidocaproyl-Val-Cit-PAB-α-amanitin</t> and ( C ) <t>azide-PEG4-Val-Cit-PAB-monomethyl</t> auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.
    Azide Peg4 Val Cit Pab Monomethyl Auristatin E, supplied by Levena Biopharma, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azide peg4 val cit pab monomethyl auristatin e/product/Levena Biopharma
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    azide peg4 val cit pab monomethyl auristatin e - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    85
    Innovative Therapies itcc cit
    JARID2 is more highly expressed in fusion positive RMS patients and correlates with metastasis and poor clinical outcome ( A ) <t>ITCC/CIT</t> affymetrix microarray expression profiling data for RMS fusion gene positive and fusion gene negative patient samples (n=101). These were analyzed for fold differences (FD) by T-test (p
    Itcc Cit, supplied by Innovative Therapies, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/itcc cit/product/Innovative Therapies
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    itcc cit - by Bioz Stars, 2020-11
    85/100 stars
      Buy from Supplier

    99
    Abcam histone h3
    TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated <t>Histone</t> H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and
    Histone H3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 6615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/histone h3/product/Abcam
    Average 99 stars, based on 6615 article reviews
    Price from $9.99 to $1999.99
    histone h3 - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    99
    Becton Dickinson mouse monoclonal anti cit k
    TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated <t>Histone</t> H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and
    Mouse Monoclonal Anti Cit K, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti cit k/product/Becton Dickinson
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti cit k - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    94
    Abcam anti cit antibody
    TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated <t>Histone</t> H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and
    Anti Cit Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cit antibody/product/Abcam
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti cit antibody - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    98
    Abcam anti cit histone h3 ab
    TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated <t>Histone</t> H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and
    Anti Cit Histone H3 Ab, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cit histone h3 ab/product/Abcam
    Average 98 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti cit histone h3 ab - by Bioz Stars, 2020-11
    98/100 stars
      Buy from Supplier

    96
    Abnova anti cit mouse polyclonal antibody
    TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated <t>Histone</t> H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and
    Anti Cit Mouse Polyclonal Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cit mouse polyclonal antibody/product/Abnova
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cit mouse polyclonal antibody - by Bioz Stars, 2020-11
    96/100 stars
      Buy from Supplier

    89
    Drucker Diagnostics cit
    TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated <t>Histone</t> H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and
    Cit, supplied by Drucker Diagnostics, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cit/product/Drucker Diagnostics
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cit - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

    89
    Thermo Fisher cit
    The RR domain is dispensable for AID association with tightly held nuclear complexes. a Representative confocal microscopic images of <t>CH12</t> B cells either fixed directly (whole cell) or after nuclear washing (0.5% Triton+200 mM NaCl). Isolated nuclei were analysed by IF to detect endogenous AID and Spt5 and DNA stained by Dapi. Cells were stimulated <t>(+CIT)</t> to induce AID expression or not (−CIT). Representative of three experiments. b (Left) Representative confocal microscopic images of GFP, AID (detected by IF) and DNA (Dapi) in reconstituted AID-deficient CH12 B cells, imaged as in a . AID and GFP expression were linked via IRES. (Right) Nuclear AID signal was calculated relative to whole-cell intensity for each variant and normalized to the wt AID value. Plotted are means (bars) from 3 independent experiments (dots) with 20–77 cells per condition per experiment. a , b Magnification 630×. Scale bar, 10 μm. Laser power and/or gain were increased for imaging after nuclear wash (see Methods). c Scheme for biochemical fractionation of B cells. Fractions analysed are indicated in brackets. d . e Quantification of AID signal in each lane from d , normalized to the respective cytoplasmic AID. Means + s.d. from three independent experiments
    Cit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cit/product/Thermo Fisher
    Average 89 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    cit - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

    89
    Toronto Research Chemicals cit
    A schematic depiction of the experimental procedure in experiment 1. CAR, conditioned avoidance response; <t>CIT,</t> citalopram; <t>HAL,</t> haloperidol; VEH, vehicle.
    Cit, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cit/product/Toronto Research Chemicals
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cit - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

    85
    Abcam citrullinated histone h3 cit h3
    A schematic depiction of the experimental procedure in experiment 1. CAR, conditioned avoidance response; <t>CIT,</t> citalopram; <t>HAL,</t> haloperidol; VEH, vehicle.
    Citrullinated Histone H3 Cit H3, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/citrullinated histone h3 cit h3/product/Abcam
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    citrullinated histone h3 cit h3 - by Bioz Stars, 2020-11
    85/100 stars
      Buy from Supplier

    92
    Abcam rabbit anti cit h3 antibody
    Alvelestat or DNase attenuated NET formation in aspiration-induced ARDS model ( A ) NETs were located by confocal microscopy of lung section with immunofluorescence in the sham group, HCl group, HCl plus alvelestat group and HCl plus DNase group at 12 h. Visualization of the complex of <t>cit-H3</t> and NE (NETs, white arrow) was reduced in mice after administration of alvelestat and DNase. Scale bars: 50 μm. ( B ) An example of cit-H3 and tubulin detected by Western blot in the lung tissues of each group, and the cit-H3/tubulin ratio ( C ) decreased in mice with administration of alvelestat and DNase, with parallel results of NET-DNA in BALF(D). ( * p
    Rabbit Anti Cit H3 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cit h3 antibody/product/Abcam
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cit h3 antibody - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    92
    Levena Biopharma maleimid caproyl val cit pab α amanitin
    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to <t>α-amanitin</t> and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of <t>maleimidocaproyl-Val-Cit-PAB-α-amanitin</t> and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.
    Maleimid Caproyl Val Cit Pab α Amanitin, supplied by Levena Biopharma, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maleimid caproyl val cit pab α amanitin/product/Levena Biopharma
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    maleimid caproyl val cit pab α amanitin - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    91
    Levena Biopharma maleimidocaproyl val cit pab mmae
    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to <t>α-amanitin</t> and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of <t>maleimidocaproyl-Val-Cit-PAB-α-amanitin</t> and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.
    Maleimidocaproyl Val Cit Pab Mmae, supplied by Levena Biopharma, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maleimidocaproyl val cit pab mmae/product/Levena Biopharma
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    maleimidocaproyl val cit pab mmae - by Bioz Stars, 2020-11
    91/100 stars
      Buy from Supplier

    93
    4Gene pndm cit
    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to <t>α-amanitin</t> and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of <t>maleimidocaproyl-Val-Cit-PAB-α-amanitin</t> and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.
    Pndm Cit, supplied by 4Gene, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pndm cit/product/4Gene
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pndm cit - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

    92
    R-Biopharm ridascreen fast cit elisa kit
    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to <t>α-amanitin</t> and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of <t>maleimidocaproyl-Val-Cit-PAB-α-amanitin</t> and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.
    Ridascreen Fast Cit Elisa Kit, supplied by R-Biopharm, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ridascreen fast cit elisa kit/product/R-Biopharm
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ridascreen fast cit elisa kit - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    85
    Tocris 3h □ cit
    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to <t>α-amanitin</t> and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of <t>maleimidocaproyl-Val-Cit-PAB-α-amanitin</t> and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.
    3h □ Cit, supplied by Tocris, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3h □ cit/product/Tocris
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    3h □ cit - by Bioz Stars, 2020-11
    85/100 stars
      Buy from Supplier

    Image Search Results


    Immunohistochemical observation of NETs and fibrin markers in thick fibrils in inflammatory exudates in legionnaire’s pneumonia ( a–f ) and liver abscess ( g–l ). HE ( a, g ), Cit-H3 ( b, h ), LF ( c, i ), MPO ( d, j ), NE ( e, k ) and FGG ( f, l ). Thick fibrils in legionnaire’s pneumonia show immunoreactivities of all the NETs markers and FGG ( b–f ). Thick fibrils in liver abscess are immunoreactive only for FGG ( l ). Neutrophils intermingled within the meshwork are labeled for the neutrophil markers. Dotted circles represent HE-stained thick fibrils. Bar=50 μm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: I. Light Microscopic Study

    doi: 10.1267/ahc.16015

    Figure Lengend Snippet: Immunohistochemical observation of NETs and fibrin markers in thick fibrils in inflammatory exudates in legionnaire’s pneumonia ( a–f ) and liver abscess ( g–l ). HE ( a, g ), Cit-H3 ( b, h ), LF ( c, i ), MPO ( d, j ), NE ( e, k ) and FGG ( f, l ). Thick fibrils in legionnaire’s pneumonia show immunoreactivities of all the NETs markers and FGG ( b–f ). Thick fibrils in liver abscess are immunoreactive only for FGG ( l ). Neutrophils intermingled within the meshwork are labeled for the neutrophil markers. Dotted circles represent HE-stained thick fibrils. Bar=50 μm.

    Article Snippet: Cit-H3 (rabbit polyclonal, at 1:100 dilution, Abcam, Eugene, OR, USA), LF (rabbit polyclonal, at 1:300 dilution, GenWay Biotech, San Diego, CA, USA), MPO (rabbit polyclonal, at 1:2,000 dilution, Dako, Glostrup, Denmark), NE (rabbit polyclonal, at 1:400 dilution, Abcam), FGG (mouse monoclonal, clone 1F2, at 1:300 dilution, Abnova, Taipei, Taiwan), CC3 (rabbit polyclonal , at 1:100 dilution; Cell Signaling Technology, Danvers, MA, USA) and fractin (rabbit polyclonal, at 1:100 dilution , Abcam).

    Techniques: Immunohistochemistry, Labeling, Staining

    Immunohistochemical observation of NETs and fibrin markers in thin fibrils in inflammatory exudates in acute appendicitis ( a–f ) and acute cholecystitis ( g–l ). HE ( a, g ), Cit-H3 ( b, h ), LF ( c, i ), MPO ( d, j ), NE ( e, k ) and FGG ( f, l ). Eosinophilic thin fibrils ( a ) show immunoreactivities of all NETs markers ( b–e ), while FGG is negative ( f ). Chromatin-rich basophilic thin fibrils ( g ) express NETs immunoreactivities ( i–k ). Cit-H3 and FGG are negative ( h, l ). Neutrophils intermingled within the meshwork are labeled for the neutrophil markers (Cit-H3 in the nuclei and LF, MPO and NE in the cytoplasm). Dotted circles represent HE-stained thin fibrils. Bar=50 μm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: I. Light Microscopic Study

    doi: 10.1267/ahc.16015

    Figure Lengend Snippet: Immunohistochemical observation of NETs and fibrin markers in thin fibrils in inflammatory exudates in acute appendicitis ( a–f ) and acute cholecystitis ( g–l ). HE ( a, g ), Cit-H3 ( b, h ), LF ( c, i ), MPO ( d, j ), NE ( e, k ) and FGG ( f, l ). Eosinophilic thin fibrils ( a ) show immunoreactivities of all NETs markers ( b–e ), while FGG is negative ( f ). Chromatin-rich basophilic thin fibrils ( g ) express NETs immunoreactivities ( i–k ). Cit-H3 and FGG are negative ( h, l ). Neutrophils intermingled within the meshwork are labeled for the neutrophil markers (Cit-H3 in the nuclei and LF, MPO and NE in the cytoplasm). Dotted circles represent HE-stained thin fibrils. Bar=50 μm.

    Article Snippet: Cit-H3 (rabbit polyclonal, at 1:100 dilution, Abcam, Eugene, OR, USA), LF (rabbit polyclonal, at 1:300 dilution, GenWay Biotech, San Diego, CA, USA), MPO (rabbit polyclonal, at 1:2,000 dilution, Dako, Glostrup, Denmark), NE (rabbit polyclonal, at 1:400 dilution, Abcam), FGG (mouse monoclonal, clone 1F2, at 1:300 dilution, Abnova, Taipei, Taiwan), CC3 (rabbit polyclonal , at 1:100 dilution; Cell Signaling Technology, Danvers, MA, USA) and fractin (rabbit polyclonal, at 1:100 dilution , Abcam).

    Techniques: Immunohistochemistry, Labeling, Staining

    Involvement of apoptosis in NETs-positive inflammatory exudates in lung abscess. HE ( a ), Cit-H3 ( b ), LF ( c ), MPO ( d ), NE ( e ), CC3 ( f ), fractin ( g ) and FGG ( h ). Cytoplasmic positivity of CC3 and fractin is scattered among the inflammatory cells ( f, g ). The background thick fibrils are immunoreactive for Cit-H3, LF and FGG ( b, c, h ). MPO and NE immunoreactivities were undetectable on the fibrils ( d, e ). The nuclei and cytoplasm of neutrophils are labeled for Cit-H3 and LF, MPO and NE, respectively. Bar=50 μm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: I. Light Microscopic Study

    doi: 10.1267/ahc.16015

    Figure Lengend Snippet: Involvement of apoptosis in NETs-positive inflammatory exudates in lung abscess. HE ( a ), Cit-H3 ( b ), LF ( c ), MPO ( d ), NE ( e ), CC3 ( f ), fractin ( g ) and FGG ( h ). Cytoplasmic positivity of CC3 and fractin is scattered among the inflammatory cells ( f, g ). The background thick fibrils are immunoreactive for Cit-H3, LF and FGG ( b, c, h ). MPO and NE immunoreactivities were undetectable on the fibrils ( d, e ). The nuclei and cytoplasm of neutrophils are labeled for Cit-H3 and LF, MPO and NE, respectively. Bar=50 μm.

    Article Snippet: Cit-H3 (rabbit polyclonal, at 1:100 dilution, Abcam, Eugene, OR, USA), LF (rabbit polyclonal, at 1:300 dilution, GenWay Biotech, San Diego, CA, USA), MPO (rabbit polyclonal, at 1:2,000 dilution, Dako, Glostrup, Denmark), NE (rabbit polyclonal, at 1:400 dilution, Abcam), FGG (mouse monoclonal, clone 1F2, at 1:300 dilution, Abnova, Taipei, Taiwan), CC3 (rabbit polyclonal , at 1:100 dilution; Cell Signaling Technology, Danvers, MA, USA) and fractin (rabbit polyclonal, at 1:100 dilution , Abcam).

    Techniques: Labeling

    Immunohistochemical observation of NETs and fibrin markers in clustered thick fibrils in lobar pneumonia. HE ( a ), Cit-H3 ( b ), LF ( c ), MPO ( d ), NE ( e ) and FGG ( f ). Clustered thick fibrils seen in lobar pneumonia solely exhibit immunoreactivity of FGG ( f ). Neutrophils are scarcely seen among the deposit. Dotted circles represent HE-stained clustered thick fibrils. Bar=50 μm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: I. Light Microscopic Study

    doi: 10.1267/ahc.16015

    Figure Lengend Snippet: Immunohistochemical observation of NETs and fibrin markers in clustered thick fibrils in lobar pneumonia. HE ( a ), Cit-H3 ( b ), LF ( c ), MPO ( d ), NE ( e ) and FGG ( f ). Clustered thick fibrils seen in lobar pneumonia solely exhibit immunoreactivity of FGG ( f ). Neutrophils are scarcely seen among the deposit. Dotted circles represent HE-stained clustered thick fibrils. Bar=50 μm.

    Article Snippet: Cit-H3 (rabbit polyclonal, at 1:100 dilution, Abcam, Eugene, OR, USA), LF (rabbit polyclonal, at 1:300 dilution, GenWay Biotech, San Diego, CA, USA), MPO (rabbit polyclonal, at 1:2,000 dilution, Dako, Glostrup, Denmark), NE (rabbit polyclonal, at 1:400 dilution, Abcam), FGG (mouse monoclonal, clone 1F2, at 1:300 dilution, Abnova, Taipei, Taiwan), CC3 (rabbit polyclonal , at 1:100 dilution; Cell Signaling Technology, Danvers, MA, USA) and fractin (rabbit polyclonal, at 1:100 dilution , Abcam).

    Techniques: Immunohistochemistry, Staining

    The immunofluorescence findings of NETs in the kidney and skin purpura. The other lesions of glomerulitis (A-F) and cutaneous small arteries of the skin purpura (G-L). (A, G) Hematoxylin and Eosin staining; (B, H) Cit-H3 staining with anti-Cit-H3 and Alexa Fluor 594-conjugated goat anti-rabbit IgG H L (red); (C, I) MPO staining with FITC-conjugated anti-MPO (green); (D, J) DNA staining with DAPI (blue). Cit-H3 and extracellular DNA were co-localized with MPO in the glomeruli (F), but not in the cutaneous arteries (L). The small arteries of the gastric and duodenal ulcers were negative for Cit-H3.

    Journal: Internal Medicine

    Article Title: An Autopsy Case of Myeloperoxidase-anti-neutrophil Cytoplasmic Antibody (MPO-ANCA)-associated Vasculitis Accompanied by Cryoglobulinemic Vasculitis Affecting the Kidneys, Skin, and Gastrointestinal Tract

    doi: 10.2169/internalmedicine.0720-17

    Figure Lengend Snippet: The immunofluorescence findings of NETs in the kidney and skin purpura. The other lesions of glomerulitis (A-F) and cutaneous small arteries of the skin purpura (G-L). (A, G) Hematoxylin and Eosin staining; (B, H) Cit-H3 staining with anti-Cit-H3 and Alexa Fluor 594-conjugated goat anti-rabbit IgG H L (red); (C, I) MPO staining with FITC-conjugated anti-MPO (green); (D, J) DNA staining with DAPI (blue). Cit-H3 and extracellular DNA were co-localized with MPO in the glomeruli (F), but not in the cutaneous arteries (L). The small arteries of the gastric and duodenal ulcers were negative for Cit-H3.

    Article Snippet: We used immunofluorescence to detect citrullinated histone 3 (Cit-H3) using anti-Cit-H3 (Abcam, ab5103) and goat anti-rabbit IgG H & L (Abcam, ab150080), DNA stained with 4'6-diamidino-2-phenylindole (DAPI), and MPO stained with anti-MPO (Gene Tex, GTX11729) to visualize NET formation in the skin biopsy and autopsy specimens ( ).

    Techniques: Immunofluorescence, Staining

    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to α-amanitin and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of maleimidocaproyl-Val-Cit-PAB-α-amanitin and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.

    Journal: International Journal of Molecular Sciences

    Article Title: FGF2 Dual Warhead Conjugate with Monomethyl Auristatin E and α-Amanitin Displays a Cytotoxic Effect towards Cancer Cells Overproducing FGF Receptor 1

    doi: 10.3390/ijms19072098

    Figure Lengend Snippet: Site-specific conjugation of fibroblast growth factor 2 (FGF2) to α-amanitin and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of maleimidocaproyl-Val-Cit-PAB-α-amanitin and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.

    Article Snippet: The FGF2 Cys96PrK variant (20 µM, 10 nmol) was reacted with azide-PEG4-Val-Cit-PAB-monomethyl auristatin E (Levena Biopharma, San Diego, CA, USA) in Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition.

    Techniques: Conjugation Assay, SDS Page, Mass Spectrometry

    JARID2 is more highly expressed in fusion positive RMS patients and correlates with metastasis and poor clinical outcome ( A ) ITCC/CIT affymetrix microarray expression profiling data for RMS fusion gene positive and fusion gene negative patient samples (n=101). These were analyzed for fold differences (FD) by T-test (p

    Journal: Oncogene

    Article Title: JARID2 is a direct target of the PAX3-FOXO1 fusion protein and inhibits myogenic differentiation of rhabdomyosarcoma cells

    doi: 10.1038/onc.2013.46

    Figure Lengend Snippet: JARID2 is more highly expressed in fusion positive RMS patients and correlates with metastasis and poor clinical outcome ( A ) ITCC/CIT affymetrix microarray expression profiling data for RMS fusion gene positive and fusion gene negative patient samples (n=101). These were analyzed for fold differences (FD) by T-test (p

    Article Snippet: Histone demethylases are highly expressed in rhabdomyosarcomas To identify HDM gene family members that may be involved in maintaining the undifferentiated myogenic phenotype of RMS, we assessed mRNA levels of 32 HDMs in a panel of 101 RMS patient samples relative to 30 skeletal muscle samples using our previously published patient ITCC/CIT dataset (Innovative Therapies for Children with Cancer/Carte d’Identité des Tumeurs) and publicly available Affymetrix expression profiling data for skeletal muscle (SkM), embryonic (ESC) and mesenchymal (MSC) stem cells.

    Techniques: Microarray, Expressing

    TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated Histone H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and

    Journal: Molecular Medicine

    Article Title: Superoxide Induces Neutrophil Extracellular Trap Formation in a TLR-4 and NOX-Dependent Mechanism

    doi: 10.2119/molmed.2016.00054

    Figure Lengend Snippet: TLR-4 mediates NET induction by extracellular superoxide. (A) Flow cytometry analysis of citrullinated Histone H3 in WT neutrophils after treatment with media alone (control), eritoran TLR-4 antagonist (8 ng/mL), xanthine oxidase (10 mU/mL) and

    Article Snippet: Prior to chromatin extrusion as a NET web, citrullination of histone H3 (cit-H3) by the enzyme peptidyl-arginine-deiminase 4 (PAD4) is required to induce decondensation. ( , ) To further evaluate NET formation to superoxide, we measured this modification specific for NETs by western blot.

    Techniques: Flow Cytometry, Cytometry

    Histone-induced toxicity in mice. ( A ) Hematoxylin and eosin (H E)-stained sections: ( a ) lung from an untreated mouse and ( b ) lung, ( c ) liver, and ( d ) kidney from a mouse 4 hours after infusion with histones (60 mg/kg). Obvious pathological changes were found in the lungs, such as edema ( black arrows ), microvascular congestion ( red arrow ), and hemorrhage ( blue arrow ) ( b ). Less obvious changes were found in ( c ) the liver and ( d ) kidneys. ( B ) Survival curves of mice injected with calf thymus (Cth) histones (75 mg/kg; a lethal dose) preincubated without (none of seven survived) or with anti-histone scFv (ahscFv, 10 mg/kg) (seven of seven survived) and control scFv (cscFv, 10 mg/ml) (none of seven survived); log-rank test, P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Circulating Histones Are Mediators of Trauma-associated Lung Injury

    doi: 10.1164/rccm.201206-1037OC

    Figure Lengend Snippet: Histone-induced toxicity in mice. ( A ) Hematoxylin and eosin (H E)-stained sections: ( a ) lung from an untreated mouse and ( b ) lung, ( c ) liver, and ( d ) kidney from a mouse 4 hours after infusion with histones (60 mg/kg). Obvious pathological changes were found in the lungs, such as edema ( black arrows ), microvascular congestion ( red arrow ), and hemorrhage ( blue arrow ) ( b ). Less obvious changes were found in ( c ) the liver and ( d ) kidneys. ( B ) Survival curves of mice injected with calf thymus (Cth) histones (75 mg/kg; a lethal dose) preincubated without (none of seven survived) or with anti-histone scFv (ahscFv, 10 mg/kg) (seven of seven survived) and control scFv (cscFv, 10 mg/ml) (none of seven survived); log-rank test, P

    Article Snippet: After antigen retrieval with the PT link for pre-treatment system (Dako, Glostrup, Denmark), paraffin-embedded sections were stained with anti–histone H3, anti–citrullinated (cit) histone H3, and anti-MPO (Abcam, Cambridge, UK), and with anti-fibrin and an EnVision kit (Dako).

    Techniques: Mouse Assay, Staining, Injection

    Membrane binding and calcium influx determine histone toxicity in endothelial cells. ( A ) Confocal images of EA.hy926 cells 10 minutes after incubation with fluorescein isothiocyanate (FITC)–labeled histones (10 μg/ml) alone ( left ) or FITC-labeled histones preincubated with anti-histone scFv (ahscFv; 100 μg/ml) ( right ). Arrows indicate the membrane associated with FITC-labeled histones. Scale bar: 20 μm. ( B ) Immunohistochemical staining of histone H3 in tissues from a mouse 4 hours after infusion with histones at 60 mg/kg ( left ) and an untreated mouse ( right ). Red arrows indicate endothelial nuclei and blue arrows point to a continuous line between endothelial nuclei stained with anti-histones to indicate association of histones with the plasma membranes of endothelial cells. Scale bar: 20 μm. ( C ) Representative whole-cell currents recorded from EA.hy926 cells when histones (20 μg/ml) were applied to the extracellular bathing solution. Currents generated by application of histones were reversible by washing after a short exposure (30–60 s). ( D ) Example of elevation of intracellular Ca 2+ recorded with a Hitachi F-7000 fluorescence spectrometer when EA.hy926 cells were exposed to various histone concentrations. ( E ) Example of intracellular Ca 2+ elevation triggered by histones (20 μg/ml) that was nearly abolished by removal of Ca 2+ from extracellular medium. ( F ) Survival rates (mean ± SD) of cells incubated for 1 hour with medium containing 0–3 mM Ca 2+ in the presence or absence of histones (20 μg/ml), from three independent experiments. *Significant reduction in cell survival rate compared with that without Ca 2+ (analysis of variance test, P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Circulating Histones Are Mediators of Trauma-associated Lung Injury

    doi: 10.1164/rccm.201206-1037OC

    Figure Lengend Snippet: Membrane binding and calcium influx determine histone toxicity in endothelial cells. ( A ) Confocal images of EA.hy926 cells 10 minutes after incubation with fluorescein isothiocyanate (FITC)–labeled histones (10 μg/ml) alone ( left ) or FITC-labeled histones preincubated with anti-histone scFv (ahscFv; 100 μg/ml) ( right ). Arrows indicate the membrane associated with FITC-labeled histones. Scale bar: 20 μm. ( B ) Immunohistochemical staining of histone H3 in tissues from a mouse 4 hours after infusion with histones at 60 mg/kg ( left ) and an untreated mouse ( right ). Red arrows indicate endothelial nuclei and blue arrows point to a continuous line between endothelial nuclei stained with anti-histones to indicate association of histones with the plasma membranes of endothelial cells. Scale bar: 20 μm. ( C ) Representative whole-cell currents recorded from EA.hy926 cells when histones (20 μg/ml) were applied to the extracellular bathing solution. Currents generated by application of histones were reversible by washing after a short exposure (30–60 s). ( D ) Example of elevation of intracellular Ca 2+ recorded with a Hitachi F-7000 fluorescence spectrometer when EA.hy926 cells were exposed to various histone concentrations. ( E ) Example of intracellular Ca 2+ elevation triggered by histones (20 μg/ml) that was nearly abolished by removal of Ca 2+ from extracellular medium. ( F ) Survival rates (mean ± SD) of cells incubated for 1 hour with medium containing 0–3 mM Ca 2+ in the presence or absence of histones (20 μg/ml), from three independent experiments. *Significant reduction in cell survival rate compared with that without Ca 2+ (analysis of variance test, P

    Article Snippet: After antigen retrieval with the PT link for pre-treatment system (Dako, Glostrup, Denmark), paraffin-embedded sections were stained with anti–histone H3, anti–citrullinated (cit) histone H3, and anti-MPO (Abcam, Cambridge, UK), and with anti-fibrin and an EnVision kit (Dako).

    Techniques: Binding Assay, Incubation, Labeling, Immunohistochemistry, Staining, Generated, Fluorescence

    Histone-induced coagulation activation in vivo . ( A ) Box plot shows the medians of thrombin–anti-thrombin (TAT) levels in 20 healthy donors (Normal) and 52 patients with severe nonthoracic trauma. *Median test, P = 0.001. ( B ) Mean ± SD of TAT in mice infused with saline (Control), calf thymus histones (Histones, 50 mg/ml), or histones plus anti-histone scFv (ahscFv, 10 mg/kg) (Hist+ahscFv) (10 mice per group). *Analysis of variance (ANOVA) test, P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Circulating Histones Are Mediators of Trauma-associated Lung Injury

    doi: 10.1164/rccm.201206-1037OC

    Figure Lengend Snippet: Histone-induced coagulation activation in vivo . ( A ) Box plot shows the medians of thrombin–anti-thrombin (TAT) levels in 20 healthy donors (Normal) and 52 patients with severe nonthoracic trauma. *Median test, P = 0.001. ( B ) Mean ± SD of TAT in mice infused with saline (Control), calf thymus histones (Histones, 50 mg/ml), or histones plus anti-histone scFv (ahscFv, 10 mg/kg) (Hist+ahscFv) (10 mice per group). *Analysis of variance (ANOVA) test, P

    Article Snippet: After antigen retrieval with the PT link for pre-treatment system (Dako, Glostrup, Denmark), paraffin-embedded sections were stained with anti–histone H3, anti–citrullinated (cit) histone H3, and anti-MPO (Abcam, Cambridge, UK), and with anti-fibrin and an EnVision kit (Dako).

    Techniques: Coagulation, Activation Assay, In Vivo, Mouse Assay

    Histone-triggered cytokine release. ( A ) Circulating IL-6 levels in both the mouse trauma model (Trauma) and the histone infusion model with or without the coinfusion of anti-histone scFv (ahscFv, 10 mg/kg). With 10 mice per group, the means ± SD of IL-6 are shown. *Analysis of variance (ANOVA) test shows a significant increase compared with that before treatment (Before), P

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: Circulating Histones Are Mediators of Trauma-associated Lung Injury

    doi: 10.1164/rccm.201206-1037OC

    Figure Lengend Snippet: Histone-triggered cytokine release. ( A ) Circulating IL-6 levels in both the mouse trauma model (Trauma) and the histone infusion model with or without the coinfusion of anti-histone scFv (ahscFv, 10 mg/kg). With 10 mice per group, the means ± SD of IL-6 are shown. *Analysis of variance (ANOVA) test shows a significant increase compared with that before treatment (Before), P

    Article Snippet: After antigen retrieval with the PT link for pre-treatment system (Dako, Glostrup, Denmark), paraffin-embedded sections were stained with anti–histone H3, anti–citrullinated (cit) histone H3, and anti-MPO (Abcam, Cambridge, UK), and with anti-fibrin and an EnVision kit (Dako).

    Techniques: Mouse Assay

    The RR domain is dispensable for AID association with tightly held nuclear complexes. a Representative confocal microscopic images of CH12 B cells either fixed directly (whole cell) or after nuclear washing (0.5% Triton+200 mM NaCl). Isolated nuclei were analysed by IF to detect endogenous AID and Spt5 and DNA stained by Dapi. Cells were stimulated (+CIT) to induce AID expression or not (−CIT). Representative of three experiments. b (Left) Representative confocal microscopic images of GFP, AID (detected by IF) and DNA (Dapi) in reconstituted AID-deficient CH12 B cells, imaged as in a . AID and GFP expression were linked via IRES. (Right) Nuclear AID signal was calculated relative to whole-cell intensity for each variant and normalized to the wt AID value. Plotted are means (bars) from 3 independent experiments (dots) with 20–77 cells per condition per experiment. a , b Magnification 630×. Scale bar, 10 μm. Laser power and/or gain were increased for imaging after nuclear wash (see Methods). c Scheme for biochemical fractionation of B cells. Fractions analysed are indicated in brackets. d . e Quantification of AID signal in each lane from d , normalized to the respective cytoplasmic AID. Means + s.d. from three independent experiments

    Journal: Nature Communications

    Article Title: A licensing step links AID to transcription elongation for mutagenesis in B cells

    doi: 10.1038/s41467-018-03387-6

    Figure Lengend Snippet: The RR domain is dispensable for AID association with tightly held nuclear complexes. a Representative confocal microscopic images of CH12 B cells either fixed directly (whole cell) or after nuclear washing (0.5% Triton+200 mM NaCl). Isolated nuclei were analysed by IF to detect endogenous AID and Spt5 and DNA stained by Dapi. Cells were stimulated (+CIT) to induce AID expression or not (−CIT). Representative of three experiments. b (Left) Representative confocal microscopic images of GFP, AID (detected by IF) and DNA (Dapi) in reconstituted AID-deficient CH12 B cells, imaged as in a . AID and GFP expression were linked via IRES. (Right) Nuclear AID signal was calculated relative to whole-cell intensity for each variant and normalized to the wt AID value. Plotted are means (bars) from 3 independent experiments (dots) with 20–77 cells per condition per experiment. a , b Magnification 630×. Scale bar, 10 μm. Laser power and/or gain were increased for imaging after nuclear wash (see Methods). c Scheme for biochemical fractionation of B cells. Fractions analysed are indicated in brackets. d . e Quantification of AID signal in each lane from d , normalized to the respective cytoplasmic AID. Means + s.d. from three independent experiments

    Article Snippet: CSR in CH12 B cells was induced with CIT [1 μg mL−1 rat-anti-CD40 (clone 1C10, eBioscience), 10 ng mL−1 interleukin (IL)-4 and 1 ng mL−1 transforming growth factor-β1 (R & D Systems)].

    Techniques: Isolation, Staining, Expressing, Variant Assay, Imaging, Fractionation

    A schematic depiction of the experimental procedure in experiment 1. CAR, conditioned avoidance response; CIT, citalopram; HAL, haloperidol; VEH, vehicle.

    Journal: Behavioural pharmacology

    Article Title: Drug–drug conditioning between citalopram and haloperidol or olanzapine in a conditioned avoidance response model: implications for polypharmacy in schizophrenia

    doi: 10.1097/FBP.0b013e328358590d

    Figure Lengend Snippet: A schematic depiction of the experimental procedure in experiment 1. CAR, conditioned avoidance response; CIT, citalopram; HAL, haloperidol; VEH, vehicle.

    Article Snippet: The injection solutions of HAL (5 mg/ml ampoules, Shanghai Xudong Haipu Pharmaceutical Co., Shanghai, China) and CIT (Toronto Research Chemicals Inc., Toronto, Ontario, Canada) were obtained by mixing drugs with sterile water.

    Techniques:

    Alvelestat or DNase attenuated NET formation in aspiration-induced ARDS model ( A ) NETs were located by confocal microscopy of lung section with immunofluorescence in the sham group, HCl group, HCl plus alvelestat group and HCl plus DNase group at 12 h. Visualization of the complex of cit-H3 and NE (NETs, white arrow) was reduced in mice after administration of alvelestat and DNase. Scale bars: 50 μm. ( B ) An example of cit-H3 and tubulin detected by Western blot in the lung tissues of each group, and the cit-H3/tubulin ratio ( C ) decreased in mice with administration of alvelestat and DNase, with parallel results of NET-DNA in BALF(D). ( * p

    Journal: Oncotarget

    Article Title: Neutrophil extracellular traps contribute to the pathogenesis of acid-aspiration-induced ALI/ARDS

    doi: 10.18632/oncotarget.22744

    Figure Lengend Snippet: Alvelestat or DNase attenuated NET formation in aspiration-induced ARDS model ( A ) NETs were located by confocal microscopy of lung section with immunofluorescence in the sham group, HCl group, HCl plus alvelestat group and HCl plus DNase group at 12 h. Visualization of the complex of cit-H3 and NE (NETs, white arrow) was reduced in mice after administration of alvelestat and DNase. Scale bars: 50 μm. ( B ) An example of cit-H3 and tubulin detected by Western blot in the lung tissues of each group, and the cit-H3/tubulin ratio ( C ) decreased in mice with administration of alvelestat and DNase, with parallel results of NET-DNA in BALF(D). ( * p

    Article Snippet: For cit-H3 and NE detection, slides were blocked with PBS containing 2% BSA and 3% donkey serum and then incubated for 2 h at room temperature (RT) with rabbit anti-cit H3 antibody (Abcam) and goat anti-NE antibody (Santa Cruz) as primary antibodies.

    Techniques: Confocal Microscopy, Immunofluorescence, Mouse Assay, Western Blot

    NETs were observed to determine the clinical relevance to disease severity in gastric aspiration-induced ARDS patients NETs (white arrow in D ) were characterized with cit-H3 ( A ), NE ( B ) and DNA ( C ) in bronchial aspirates from gastric aspiration-induced ARDS patients. ( E ) PaO 2 /FiO 2 is highly correlated to NET concentration in bronchial aspirate from gastric aspiration-induced ARDS patients.

    Journal: Oncotarget

    Article Title: Neutrophil extracellular traps contribute to the pathogenesis of acid-aspiration-induced ALI/ARDS

    doi: 10.18632/oncotarget.22744

    Figure Lengend Snippet: NETs were observed to determine the clinical relevance to disease severity in gastric aspiration-induced ARDS patients NETs (white arrow in D ) were characterized with cit-H3 ( A ), NE ( B ) and DNA ( C ) in bronchial aspirates from gastric aspiration-induced ARDS patients. ( E ) PaO 2 /FiO 2 is highly correlated to NET concentration in bronchial aspirate from gastric aspiration-induced ARDS patients.

    Article Snippet: For cit-H3 and NE detection, slides were blocked with PBS containing 2% BSA and 3% donkey serum and then incubated for 2 h at room temperature (RT) with rabbit anti-cit H3 antibody (Abcam) and goat anti-NE antibody (Santa Cruz) as primary antibodies.

    Techniques: Concentration Assay

    Site-specific conjugation of fibroblast growth factor 2 (FGF2) to α-amanitin and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of maleimidocaproyl-Val-Cit-PAB-α-amanitin and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.

    Journal: International Journal of Molecular Sciences

    Article Title: FGF2 Dual Warhead Conjugate with Monomethyl Auristatin E and α-Amanitin Displays a Cytotoxic Effect towards Cancer Cells Overproducing FGF Receptor 1

    doi: 10.3390/ijms19072098

    Figure Lengend Snippet: Site-specific conjugation of fibroblast growth factor 2 (FGF2) to α-amanitin and monomethyl auristatin E (MMAE). ( A ) Schematic representation of a site-specific dual conjugation by thiol-maleimide and Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions; ( B ) Chemical structure of maleimidocaproyl-Val-Cit-PAB-α-amanitin and ( C ) azide-PEG4-Val-Cit-PAB-monomethyl auristatin E; ( D ) SDS-PAGE analysis confirmed the purity of obtained conjugates; ( E ) Mass spectrometry (MS) analysis of doubly conjugated FGF2 shows attachment of one α-amanitin and one MMAE compound per one protein molecule.

    Article Snippet: Thiol-maleimide reaction with FGF2 Cys96PrK variant (20 µM, 10 nmol) and maleimid caproyl-Val-Cit-PAB-α-amanitin (5 equiv, 100 µM, 50 nmol; Levena Biopharma, San Diego, CA, USA) was performed in 10 mM phosphate buffer pH 6.8 with 100 mM NaCl for 60 min at room temperature.

    Techniques: Conjugation Assay, SDS Page, Mass Spectrometry