Article Title: Blockade of MCP-1/CCR4 signaling-induced recruitment of activated regulatory cells evokes an antitumor immune response in head and neck squamous cell carcinoma
Figure Lengend Snippet: MCP-1 is increased in tumor tissues from HNSCC patients ( A ) Map of antibodies against chemokines on the RayBiotech Human Chemokine Antibody Array (AAH-CHE-G1). ( B ) Cell lysates from tumor and adjacent nontumor tissues were first applied to the array. Bound chemokines were then recognized by a pool of anti-chemokine antibodies corresponding to the antibodies spotted on the array. Similar results were observed in two independent arrays. ( C ) Semiquantification of scanned antibody arrays. The levels were normalized to internal positive controls present in each membrane. Semiquantitative levels are represented in the heat map. ( D ) Expression of CCR4, CCR5, CCR6, CCR7, CXCR4 ligands in tumor and adjacent nontumor tissues. Statistical differences were analyzed using Mann-Whitney U -tests. ( E ) Representative staining of HNSCC cases for MDC, TARC, and MCP-1 ( n = 15). Brown: positive staining × 100. Lower panels are magnified images of the boxed area in the corresponding upper panel. NT: nontumor; T: tumor.
Article Snippet: Using a RayBiotech human chemokine antibody array, we showed that monocyte chemoattractant protein-1 (MCP-1), an endogenous CCR4-binding ligand, was specifically upregulated in the HNSCC microenvironment compared to the other four CCR4-binding ligands.
Techniques: Ab Array, Expressing, MANN-WHITNEY, Staining