CD68 Antibody Search Results


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  • 86
    Agilent technologies cd68
    Correlation of macrophage frequency with type of MPN. Scatter plot, <t>CD68</t> (A) and CD163 (B) frequencies in biopsies of MPN patients (PMF, PV, ET, CML) and reactive BM controls. CML and reactive BM contain few macrophages, while PMF biopsies contain higher frequencies of CD68- and CD163-positive macrophages (% per nucleated cells). PV was associated with a significant higher macrophage frequency than CML or ET. Brackets indicate significant differences between diagnostic subtypes ( p
    Cd68, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd68/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd68 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    cd68  (Abcam)
    99
    Abcam cd68
    Blocking IL-1β protected ConA-treated mice from acute hepatitis. (A) BALB/c mice ( n = 6 for each group) were pretreated with recombinant human interleukin-1 receptor antagonist (rhIL-1Ra) and subsequently exposed to ConA challenge for 12 h, then sacrificed for collecting samples. Representative H E images of liver tissues were shown (100×, magnification: 400×). (B) Serum ALT and aspartate transaminase (AST) activity were assessed. (C) Serum TNF-α was analyzed by enzyme-linked immune sorbent assay (ELISA). (D) Representative immunohistochemical images of <t>CD68</t> or CD11b staining in the liver tissue of mice. Quantification of the number of CD11b-positive cells and CD68-positive cells were obtained in four visual fields (×100) in each group. (E) Level of IL-17 in the serum and livers were assessed by ELISA. (F) Primary splenocytes were treated with 10 µg/ml rhIL-1Ra in the presence of 1 µg/ml ConA. The concentration of IL-17 in the supernatants was measured by ELISA. (G) Western blot was performed to detect JAK2, p-JAK2 (Tyr1007/1008), STAT3, and p-STAT3 (Tyr705) in the livers. Each lane represented a separate animal. The blots were representative of three experiments. Quantification of protein expression with Image J software. The data were presented as means ± SD (Student’s t -test, * p
    Cd68, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd68/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd68 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad cd68
    cbMg display a cell clearance phenotype (a) <t>CD68+</t> lysosome content (red) in IBA1+ microglia (green) in brain sections from 4/6mo wild-type mice (DAPI: blue). Scale: 10 μm. Representative image (left); quantification of lysosomal area/microglia area (right) are shown. stMg: mean=0.02437, SEM=0.01011; cbMg: mean=0.1424, SEM=0.02527; p=0.0166, F=6.245, t 2 =7.668; > 15 images/region from n=3 mice. (b) DAPI content (blue) in CD68+ lysosomes (red) of IBA1+ microglia (green) was determined using 3D reconstruction (Imaris) from 4/6mo wild-type mice. Scale: 5 μm. Representative reconstruction with side scatter views (left); quantification of percentage of microglia with DAPI+ CD68+ lysosomes (right). Arrows indicate DAPI+/CD68+ lysosome, which is shown with a zoomed in view. 3D axes are shown. stMg: mean=0.1675, SEM=0.06029; cbMg: mean=0.6960, SEM=0.0868; p=0.0300, F=2.077, t 2 =5.639; > 200 cells/region from n=3 mice. Bar graphs with individual data points show mean ± SEM, t-tests were two-tailed paired. (c) Schematic showing microglia-specific TRAP-sequencing. (d) Heatmap with hierarchical clustering distances shows the variation in the expression levels (z-scored log2 RPKM [z-score]) of 733 stMg- and 297 cbMg-enriched genes identified by TRAP (DESeq2, n=2/age/region). Selected Gene Ontology (GO) annotations (Enrichr) enriched for stMg-/cbMg-enriched genes are shown. y-axis: -log10 (p-value). Dotted lines: p=0.05. (e) Genome browser views (IGV) of normalized read counts of selected genes in stMg/cbMg by TRAP.
    Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd68/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd68 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    86
    Agilent technologies anti cd68
    Kaplan-Meier cancer-specific survival and relapse-free survival curves for total gastric cancer cases. The patients were divided into two groups according to their <t>CD169/CD68</t> ratio:
    Anti Cd68, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd68/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd68 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    N/A
    Mouse Anti Human CD68 Monoclonal IgG1
      Buy from Supplier

    N/A
    This gene encodes a 110 kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages It is a member of the lysosomal endosomal associated membrane glycoprotein LAMP
      Buy from Supplier

    N/A
    Mouse monoclonal CD68 antibody
      Buy from Supplier


    Image Search Results


    Correlation of macrophage frequency with type of MPN. Scatter plot, CD68 (A) and CD163 (B) frequencies in biopsies of MPN patients (PMF, PV, ET, CML) and reactive BM controls. CML and reactive BM contain few macrophages, while PMF biopsies contain higher frequencies of CD68- and CD163-positive macrophages (% per nucleated cells). PV was associated with a significant higher macrophage frequency than CML or ET. Brackets indicate significant differences between diagnostic subtypes ( p

    Journal: Annals of Hematology

    Article Title: Macrophage frequency in the bone marrow correlates with morphologic subtype of myeloproliferative neoplasm

    doi: 10.1007/s00277-020-04304-y

    Figure Lengend Snippet: Correlation of macrophage frequency with type of MPN. Scatter plot, CD68 (A) and CD163 (B) frequencies in biopsies of MPN patients (PMF, PV, ET, CML) and reactive BM controls. CML and reactive BM contain few macrophages, while PMF biopsies contain higher frequencies of CD68- and CD163-positive macrophages (% per nucleated cells). PV was associated with a significant higher macrophage frequency than CML or ET. Brackets indicate significant differences between diagnostic subtypes ( p

    Article Snippet: IHC was performed with primary antibodies against CD163 (clone MRQ-26, Medac, 1:1000) and CD68 (clone PGM1, Agilent, 1:100) and developed using a DAB IHC detection system on a semi-automatic immunohistochemistry stainer (Autostainer 480S; Medac, Germany).

    Techniques: Peptide Mass Fingerprinting, Diagnostic Assay

    Immunofluorescence co-staining of CD68 and CD163 in MPN biopsies. Multispectral microscopy reveals frequent co-localization of CD68- (green) and CD163 (red)-positive macrophages, nuclei highlighted in DAPI (blue), representative images (× 200) (white arrow: CD68/CD163 double-positive macrophages)

    Journal: Annals of Hematology

    Article Title: Macrophage frequency in the bone marrow correlates with morphologic subtype of myeloproliferative neoplasm

    doi: 10.1007/s00277-020-04304-y

    Figure Lengend Snippet: Immunofluorescence co-staining of CD68 and CD163 in MPN biopsies. Multispectral microscopy reveals frequent co-localization of CD68- (green) and CD163 (red)-positive macrophages, nuclei highlighted in DAPI (blue), representative images (× 200) (white arrow: CD68/CD163 double-positive macrophages)

    Article Snippet: IHC was performed with primary antibodies against CD163 (clone MRQ-26, Medac, 1:1000) and CD68 (clone PGM1, Agilent, 1:100) and developed using a DAB IHC detection system on a semi-automatic immunohistochemistry stainer (Autostainer 480S; Medac, Germany).

    Techniques: Immunofluorescence, Staining, Microscopy

    Correlation of CD68-positive and CD163-expressing macrophage frequency. Macrophage frequencies determined by CD68 and CD163 IHC staining (immunopositive cells per all nucleated cells, QuPath analysis)

    Journal: Annals of Hematology

    Article Title: Macrophage frequency in the bone marrow correlates with morphologic subtype of myeloproliferative neoplasm

    doi: 10.1007/s00277-020-04304-y

    Figure Lengend Snippet: Correlation of CD68-positive and CD163-expressing macrophage frequency. Macrophage frequencies determined by CD68 and CD163 IHC staining (immunopositive cells per all nucleated cells, QuPath analysis)

    Article Snippet: IHC was performed with primary antibodies against CD163 (clone MRQ-26, Medac, 1:1000) and CD68 (clone PGM1, Agilent, 1:100) and developed using a DAB IHC detection system on a semi-automatic immunohistochemistry stainer (Autostainer 480S; Medac, Germany).

    Techniques: Expressing, Immunohistochemistry, Staining

    Macrophage frequency and distribution in MPN and reactive bone marrow biopsies. Representative photomicrographs showing CD68-expressing macrophages by IHC (upper) and CD163 staining (lower). Percentages of CD68-positive cells determined by automatic cell counting in the upper row cases from left to right: PMF (30%), ET (2%), PV (18%), CML (2%), and control (2%). Percentages of CD163-positive cells in the lower row paired samples from left to right: PMF (38%), ET (0%), PV (23%), CML (2%), and control (2%). Evenly dispersed CD68- and CD163-expressing macrophages, with stellate morphology, are increased in Ph− MPN (especially in PMF and PV) and show a more ovaloid morphology in CML (× 400 magnification)

    Journal: Annals of Hematology

    Article Title: Macrophage frequency in the bone marrow correlates with morphologic subtype of myeloproliferative neoplasm

    doi: 10.1007/s00277-020-04304-y

    Figure Lengend Snippet: Macrophage frequency and distribution in MPN and reactive bone marrow biopsies. Representative photomicrographs showing CD68-expressing macrophages by IHC (upper) and CD163 staining (lower). Percentages of CD68-positive cells determined by automatic cell counting in the upper row cases from left to right: PMF (30%), ET (2%), PV (18%), CML (2%), and control (2%). Percentages of CD163-positive cells in the lower row paired samples from left to right: PMF (38%), ET (0%), PV (23%), CML (2%), and control (2%). Evenly dispersed CD68- and CD163-expressing macrophages, with stellate morphology, are increased in Ph− MPN (especially in PMF and PV) and show a more ovaloid morphology in CML (× 400 magnification)

    Article Snippet: IHC was performed with primary antibodies against CD163 (clone MRQ-26, Medac, 1:1000) and CD68 (clone PGM1, Agilent, 1:100) and developed using a DAB IHC detection system on a semi-automatic immunohistochemistry stainer (Autostainer 480S; Medac, Germany).

    Techniques: Expressing, Immunohistochemistry, Staining, Cell Counting, Peptide Mass Fingerprinting

    Myelofibrosis (MF) grade and macrophage frequency. Scatter plot comparing MF grade and macrophage frequency using CD68 (A) and CD163 (B) automatic cell counts (% positive cells by IHC per nucleated cells, QuPath analysis) in BM trephines. MF grade was determined using a four-tiered scale [ 16 ]: MF grade with no increase in reticulin fibers (grade 0), loose increase of reticulin (1), more diffuse and dense increase in reticulin and beginning collagen fibers (2), and dense reticulin and collagen fibrosis (3)

    Journal: Annals of Hematology

    Article Title: Macrophage frequency in the bone marrow correlates with morphologic subtype of myeloproliferative neoplasm

    doi: 10.1007/s00277-020-04304-y

    Figure Lengend Snippet: Myelofibrosis (MF) grade and macrophage frequency. Scatter plot comparing MF grade and macrophage frequency using CD68 (A) and CD163 (B) automatic cell counts (% positive cells by IHC per nucleated cells, QuPath analysis) in BM trephines. MF grade was determined using a four-tiered scale [ 16 ]: MF grade with no increase in reticulin fibers (grade 0), loose increase of reticulin (1), more diffuse and dense increase in reticulin and beginning collagen fibers (2), and dense reticulin and collagen fibrosis (3)

    Article Snippet: IHC was performed with primary antibodies against CD163 (clone MRQ-26, Medac, 1:1000) and CD68 (clone PGM1, Agilent, 1:100) and developed using a DAB IHC detection system on a semi-automatic immunohistochemistry stainer (Autostainer 480S; Medac, Germany).

    Techniques: Immunohistochemistry

    Blocking IL-1β protected ConA-treated mice from acute hepatitis. (A) BALB/c mice ( n = 6 for each group) were pretreated with recombinant human interleukin-1 receptor antagonist (rhIL-1Ra) and subsequently exposed to ConA challenge for 12 h, then sacrificed for collecting samples. Representative H E images of liver tissues were shown (100×, magnification: 400×). (B) Serum ALT and aspartate transaminase (AST) activity were assessed. (C) Serum TNF-α was analyzed by enzyme-linked immune sorbent assay (ELISA). (D) Representative immunohistochemical images of CD68 or CD11b staining in the liver tissue of mice. Quantification of the number of CD11b-positive cells and CD68-positive cells were obtained in four visual fields (×100) in each group. (E) Level of IL-17 in the serum and livers were assessed by ELISA. (F) Primary splenocytes were treated with 10 µg/ml rhIL-1Ra in the presence of 1 µg/ml ConA. The concentration of IL-17 in the supernatants was measured by ELISA. (G) Western blot was performed to detect JAK2, p-JAK2 (Tyr1007/1008), STAT3, and p-STAT3 (Tyr705) in the livers. Each lane represented a separate animal. The blots were representative of three experiments. Quantification of protein expression with Image J software. The data were presented as means ± SD (Student’s t -test, * p

    Journal: Frontiers in Immunology

    Article Title: NOD-Like Receptor Protein 3 Inflammasome-Dependent IL-1β Accelerated ConA-Induced Hepatitis

    doi: 10.3389/fimmu.2018.00758

    Figure Lengend Snippet: Blocking IL-1β protected ConA-treated mice from acute hepatitis. (A) BALB/c mice ( n = 6 for each group) were pretreated with recombinant human interleukin-1 receptor antagonist (rhIL-1Ra) and subsequently exposed to ConA challenge for 12 h, then sacrificed for collecting samples. Representative H E images of liver tissues were shown (100×, magnification: 400×). (B) Serum ALT and aspartate transaminase (AST) activity were assessed. (C) Serum TNF-α was analyzed by enzyme-linked immune sorbent assay (ELISA). (D) Representative immunohistochemical images of CD68 or CD11b staining in the liver tissue of mice. Quantification of the number of CD11b-positive cells and CD68-positive cells were obtained in four visual fields (×100) in each group. (E) Level of IL-17 in the serum and livers were assessed by ELISA. (F) Primary splenocytes were treated with 10 µg/ml rhIL-1Ra in the presence of 1 µg/ml ConA. The concentration of IL-17 in the supernatants was measured by ELISA. (G) Western blot was performed to detect JAK2, p-JAK2 (Tyr1007/1008), STAT3, and p-STAT3 (Tyr705) in the livers. Each lane represented a separate animal. The blots were representative of three experiments. Quantification of protein expression with Image J software. The data were presented as means ± SD (Student’s t -test, * p

    Article Snippet: The antibodies against CD11b (ab133357) and CD68 (ab955) were obtained from Abcam (Cambridge, UK).

    Techniques: Blocking Assay, Mouse Assay, Recombinant, AST Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining, Concentration Assay, Western Blot, Expressing, Software

    cbMg display a cell clearance phenotype (a) CD68+ lysosome content (red) in IBA1+ microglia (green) in brain sections from 4/6mo wild-type mice (DAPI: blue). Scale: 10 μm. Representative image (left); quantification of lysosomal area/microglia area (right) are shown. stMg: mean=0.02437, SEM=0.01011; cbMg: mean=0.1424, SEM=0.02527; p=0.0166, F=6.245, t 2 =7.668; > 15 images/region from n=3 mice. (b) DAPI content (blue) in CD68+ lysosomes (red) of IBA1+ microglia (green) was determined using 3D reconstruction (Imaris) from 4/6mo wild-type mice. Scale: 5 μm. Representative reconstruction with side scatter views (left); quantification of percentage of microglia with DAPI+ CD68+ lysosomes (right). Arrows indicate DAPI+/CD68+ lysosome, which is shown with a zoomed in view. 3D axes are shown. stMg: mean=0.1675, SEM=0.06029; cbMg: mean=0.6960, SEM=0.0868; p=0.0300, F=2.077, t 2 =5.639; > 200 cells/region from n=3 mice. Bar graphs with individual data points show mean ± SEM, t-tests were two-tailed paired. (c) Schematic showing microglia-specific TRAP-sequencing. (d) Heatmap with hierarchical clustering distances shows the variation in the expression levels (z-scored log2 RPKM [z-score]) of 733 stMg- and 297 cbMg-enriched genes identified by TRAP (DESeq2, n=2/age/region). Selected Gene Ontology (GO) annotations (Enrichr) enriched for stMg-/cbMg-enriched genes are shown. y-axis: -log10 (p-value). Dotted lines: p=0.05. (e) Genome browser views (IGV) of normalized read counts of selected genes in stMg/cbMg by TRAP.

    Journal: Nature neuroscience

    Article Title: Epigenetic regulation of brain region-specific microglia clearance activity

    doi: 10.1038/s41593-018-0192-3

    Figure Lengend Snippet: cbMg display a cell clearance phenotype (a) CD68+ lysosome content (red) in IBA1+ microglia (green) in brain sections from 4/6mo wild-type mice (DAPI: blue). Scale: 10 μm. Representative image (left); quantification of lysosomal area/microglia area (right) are shown. stMg: mean=0.02437, SEM=0.01011; cbMg: mean=0.1424, SEM=0.02527; p=0.0166, F=6.245, t 2 =7.668; > 15 images/region from n=3 mice. (b) DAPI content (blue) in CD68+ lysosomes (red) of IBA1+ microglia (green) was determined using 3D reconstruction (Imaris) from 4/6mo wild-type mice. Scale: 5 μm. Representative reconstruction with side scatter views (left); quantification of percentage of microglia with DAPI+ CD68+ lysosomes (right). Arrows indicate DAPI+/CD68+ lysosome, which is shown with a zoomed in view. 3D axes are shown. stMg: mean=0.1675, SEM=0.06029; cbMg: mean=0.6960, SEM=0.0868; p=0.0300, F=2.077, t 2 =5.639; > 200 cells/region from n=3 mice. Bar graphs with individual data points show mean ± SEM, t-tests were two-tailed paired. (c) Schematic showing microglia-specific TRAP-sequencing. (d) Heatmap with hierarchical clustering distances shows the variation in the expression levels (z-scored log2 RPKM [z-score]) of 733 stMg- and 297 cbMg-enriched genes identified by TRAP (DESeq2, n=2/age/region). Selected Gene Ontology (GO) annotations (Enrichr) enriched for stMg-/cbMg-enriched genes are shown. y-axis: -log10 (p-value). Dotted lines: p=0.05. (e) Genome browser views (IGV) of normalized read counts of selected genes in stMg/cbMg by TRAP.

    Article Snippet: Primary antibodies: GFP (1:2000, ab6556 and 1:500 ab13970 Abcam, Cambridge, MA) , , IBA1 (1:500, 019-19741, Wako Chemicals, Richmond, VA) , P2RY12 (1:5000, kind gift from O. Butovsky) , CD68 (1:250, MCA1957, Biorad, Hercules, CA) , NeuN (1:500, MAB377, EMD Millipore, Billerica, MA) , GFAP (1:500, G3893, Sigma) , OLIG2 (1:250, MABN50, EMD Millipore) , CD11b (1:1000, MCA711GT, Biorad, Hercules, CA) , H3K27me3 (1:500, C36B11, Cell Signaling) , CD74 (1:50, sc-5438, Santa Cruz) , MHCII (1:200, ab23990, Abcam) , ApoE (1:100, AB947, Millipore) , AXL (1:100, AF854, R & D Systems) , cCASP3 (1:400, #9661, Cell Signaling) and Ki67 (1:200, ab16667, Abcam) .

    Techniques: Mouse Assay, Two Tailed Test, Sequencing, Expressing, Significance Assay

    Kaplan-Meier cancer-specific survival and relapse-free survival curves for total gastric cancer cases. The patients were divided into two groups according to their CD169/CD68 ratio:

    Journal: Frontiers in Oncology

    Article Title: CD169 Expression on Lymph Node Macrophages Predicts in Patients With Gastric Cancer

    doi: 10.3389/fonc.2021.636751

    Figure Lengend Snippet: Kaplan-Meier cancer-specific survival and relapse-free survival curves for total gastric cancer cases. The patients were divided into two groups according to their CD169/CD68 ratio:

    Article Snippet: Anti-CD169 (clone HSn 7D2; Santa Cruz Biotechnology, CA, USA), anti- CD68 (clone PG-M; Agilent Technologies, CA, USA), and anti-CD8 (clone C8/144B; Nichirei, Tokyo, Japan) antibodies were used as primary antibodies for immunohistochemistry (IHC).

    Techniques:

    Immunohistochemistry of CD169 + and CD68 + macrophage in regional lymph nodes (RLN), and CD8 + cells in primary tumor. Scale bar = 100 μm. (A) Representative figures of immunohistochemistry (IHC) images from CD169 high and low cases are shown. Lymph node sinus macrophages (LySMs) were positive for CD68 in both two patients, although, CD169 expression differed. High infiltration of CD8 + T cells in primary tumor tissues was seen in a CD169 high case and low infiltration of CD8 + T cells in primary tumor tissues was seen in a CD169 low case. (B) Double IHC of CD68(green) and CD169(brown) showed CD169 was expressed on CD68-positive macrophages. Correlation between the number of CD8 + T cells in primary tumor tissues and CD169/CD68 ratio in LySMs were tested by Spearman’s correlation test. (C) Double IHC of CD8(green) and CD169(brown) showed the direct cell-cell interaction between LySM and T cells in sinus area. Scatter plots of total (D) and in advanced (E) gastric cancer cases were shown. RLN, regional lymph node.

    Journal: Frontiers in Oncology

    Article Title: CD169 Expression on Lymph Node Macrophages Predicts in Patients With Gastric Cancer

    doi: 10.3389/fonc.2021.636751

    Figure Lengend Snippet: Immunohistochemistry of CD169 + and CD68 + macrophage in regional lymph nodes (RLN), and CD8 + cells in primary tumor. Scale bar = 100 μm. (A) Representative figures of immunohistochemistry (IHC) images from CD169 high and low cases are shown. Lymph node sinus macrophages (LySMs) were positive for CD68 in both two patients, although, CD169 expression differed. High infiltration of CD8 + T cells in primary tumor tissues was seen in a CD169 high case and low infiltration of CD8 + T cells in primary tumor tissues was seen in a CD169 low case. (B) Double IHC of CD68(green) and CD169(brown) showed CD169 was expressed on CD68-positive macrophages. Correlation between the number of CD8 + T cells in primary tumor tissues and CD169/CD68 ratio in LySMs were tested by Spearman’s correlation test. (C) Double IHC of CD8(green) and CD169(brown) showed the direct cell-cell interaction between LySM and T cells in sinus area. Scatter plots of total (D) and in advanced (E) gastric cancer cases were shown. RLN, regional lymph node.

    Article Snippet: Anti-CD169 (clone HSn 7D2; Santa Cruz Biotechnology, CA, USA), anti- CD68 (clone PG-M; Agilent Technologies, CA, USA), and anti-CD8 (clone C8/144B; Nichirei, Tokyo, Japan) antibodies were used as primary antibodies for immunohistochemistry (IHC).

    Techniques: Immunohistochemistry, Expressing

    Kaplan-Meier cancer-specific survival (CSS) curves of CD169 + /CD68 + ratios in lymph node sinus macrophages (LySMs) of advanced gastric cancer patients with various tumor subtypes: intestinal type (A) or diffuse type (B) ; lymph node (LN) metastasis negative (C) and positive (D) ; distant metastasis negative (E) and positive (F) .

    Journal: Frontiers in Oncology

    Article Title: CD169 Expression on Lymph Node Macrophages Predicts in Patients With Gastric Cancer

    doi: 10.3389/fonc.2021.636751

    Figure Lengend Snippet: Kaplan-Meier cancer-specific survival (CSS) curves of CD169 + /CD68 + ratios in lymph node sinus macrophages (LySMs) of advanced gastric cancer patients with various tumor subtypes: intestinal type (A) or diffuse type (B) ; lymph node (LN) metastasis negative (C) and positive (D) ; distant metastasis negative (E) and positive (F) .

    Article Snippet: Anti-CD169 (clone HSn 7D2; Santa Cruz Biotechnology, CA, USA), anti- CD68 (clone PG-M; Agilent Technologies, CA, USA), and anti-CD8 (clone C8/144B; Nichirei, Tokyo, Japan) antibodies were used as primary antibodies for immunohistochemistry (IHC).

    Techniques:

    Kaplan-Meier cancer-specific survival and relapse-free survival curves for patients with advanced or early gastric cancer. For all Kaplan-Meier curves, the patients were divided into two groups according to their CD169/CD68 ratio:

    Journal: Frontiers in Oncology

    Article Title: CD169 Expression on Lymph Node Macrophages Predicts in Patients With Gastric Cancer

    doi: 10.3389/fonc.2021.636751

    Figure Lengend Snippet: Kaplan-Meier cancer-specific survival and relapse-free survival curves for patients with advanced or early gastric cancer. For all Kaplan-Meier curves, the patients were divided into two groups according to their CD169/CD68 ratio:

    Article Snippet: Anti-CD169 (clone HSn 7D2; Santa Cruz Biotechnology, CA, USA), anti- CD68 (clone PG-M; Agilent Technologies, CA, USA), and anti-CD8 (clone C8/144B; Nichirei, Tokyo, Japan) antibodies were used as primary antibodies for immunohistochemistry (IHC).

    Techniques:

    Kaplan-Meier relapse free survival (RFS) curves of CD169 + /CD68 + ratios in lymph node sinus macrophages (LySMs) of advanced gastric cancer patients with various tumor subtypes: intestinal type (A) and diffuse type (B) ; lymph node (LN) metastasis negative (C) and positive (D) ; distant metastasis negative (E) and positive (F) , tumor-stroma ratio; low (G) and high (H) .

    Journal: Frontiers in Oncology

    Article Title: CD169 Expression on Lymph Node Macrophages Predicts in Patients With Gastric Cancer

    doi: 10.3389/fonc.2021.636751

    Figure Lengend Snippet: Kaplan-Meier relapse free survival (RFS) curves of CD169 + /CD68 + ratios in lymph node sinus macrophages (LySMs) of advanced gastric cancer patients with various tumor subtypes: intestinal type (A) and diffuse type (B) ; lymph node (LN) metastasis negative (C) and positive (D) ; distant metastasis negative (E) and positive (F) , tumor-stroma ratio; low (G) and high (H) .

    Article Snippet: Anti-CD169 (clone HSn 7D2; Santa Cruz Biotechnology, CA, USA), anti- CD68 (clone PG-M; Agilent Technologies, CA, USA), and anti-CD8 (clone C8/144B; Nichirei, Tokyo, Japan) antibodies were used as primary antibodies for immunohistochemistry (IHC).

    Techniques:

    Immunohistochemistry and cell counting system. The numbers of CD169 + and CD68 + cells in regional lymph nodes (RLNs) and CD8 + cells in tumors were evaluated using HALO 2.3 as described in Materials and Methods. Scale bar = 100 μm. Representative immunohistochemistry (IHC) stains of CD169 (A) and CD8 (B) are shown. Positive cells in selected areas surrounded by yellow lines were counted automatically by HALO 2.3. (C) Number of CD169/CD68 ratio in lymph node sinus macrophages (LySMs). (D) Number of CD8 expressions in primary tumor. Patients were divided into two groups according to their CD169/CD68 ratio:

    Journal: Frontiers in Oncology

    Article Title: CD169 Expression on Lymph Node Macrophages Predicts in Patients With Gastric Cancer

    doi: 10.3389/fonc.2021.636751

    Figure Lengend Snippet: Immunohistochemistry and cell counting system. The numbers of CD169 + and CD68 + cells in regional lymph nodes (RLNs) and CD8 + cells in tumors were evaluated using HALO 2.3 as described in Materials and Methods. Scale bar = 100 μm. Representative immunohistochemistry (IHC) stains of CD169 (A) and CD8 (B) are shown. Positive cells in selected areas surrounded by yellow lines were counted automatically by HALO 2.3. (C) Number of CD169/CD68 ratio in lymph node sinus macrophages (LySMs). (D) Number of CD8 expressions in primary tumor. Patients were divided into two groups according to their CD169/CD68 ratio:

    Article Snippet: Anti-CD169 (clone HSn 7D2; Santa Cruz Biotechnology, CA, USA), anti- CD68 (clone PG-M; Agilent Technologies, CA, USA), and anti-CD8 (clone C8/144B; Nichirei, Tokyo, Japan) antibodies were used as primary antibodies for immunohistochemistry (IHC).

    Techniques: Immunohistochemistry, Cell Counting

    (A) Hematoxylin and eosin staining of tumor-stroma ratio (TSR)- low and high. Scale bar = 100 μm. (B) Number of CD8 + T cells in TSR-low or high advanced cancer tissues. Correlation between the number of CD8 + T cells in TSR-low or high tumor tissues and the CD169/CD68 ratio in lymph node sinus macrophages (LySMs) were tested by Spearman’s correlation test. Scatter plot in TSR-low advanced gastric cancer cases (C) and in TSR-high advanced gastric cancer cases (D) were shown. (E) Kaplan-Meier cancer-specific survival (CSS) curves in patients with TSR-low advanced gastric cancer. (F) Kaplan-Meier CSS curves in patients with TSR-high advanced gastric cancer.

    Journal: Frontiers in Oncology

    Article Title: CD169 Expression on Lymph Node Macrophages Predicts in Patients With Gastric Cancer

    doi: 10.3389/fonc.2021.636751

    Figure Lengend Snippet: (A) Hematoxylin and eosin staining of tumor-stroma ratio (TSR)- low and high. Scale bar = 100 μm. (B) Number of CD8 + T cells in TSR-low or high advanced cancer tissues. Correlation between the number of CD8 + T cells in TSR-low or high tumor tissues and the CD169/CD68 ratio in lymph node sinus macrophages (LySMs) were tested by Spearman’s correlation test. Scatter plot in TSR-low advanced gastric cancer cases (C) and in TSR-high advanced gastric cancer cases (D) were shown. (E) Kaplan-Meier cancer-specific survival (CSS) curves in patients with TSR-low advanced gastric cancer. (F) Kaplan-Meier CSS curves in patients with TSR-high advanced gastric cancer.

    Article Snippet: Anti-CD169 (clone HSn 7D2; Santa Cruz Biotechnology, CA, USA), anti- CD68 (clone PG-M; Agilent Technologies, CA, USA), and anti-CD8 (clone C8/144B; Nichirei, Tokyo, Japan) antibodies were used as primary antibodies for immunohistochemistry (IHC).

    Techniques: Staining