CD47 Antibody Search Results


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  • 86
    Exosome Diagnostics cd47
    The structure of <t>CD47/IAP.</t>
    Cd47, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd47/product/Exosome Diagnostics
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd47 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    97
    Bio X Cell anti cd47
    Administration of RvD1 to WD-fed Ldlr −/− mice with established atherosclerosis decreases lesional <t>CD47</t> and necrosis. Ldlr −/− mice were fed a WD for 12 weeks, after which Veh or 100 ng/mouse of RvD1 (3x/week i.p.) was administered for 3 weeks. Aortic root lesions were immunostained with anti-CD47 ( a ) or anti-p-SHP1 ( b ) antibody and counterstained with Hoechst. The mean fluorescence intensity of CD47 was quantified. c Lesional necrosis was quantified with Olympus DP2-BSW software. All results are mean ± sem, n = 8 separate mice per group. * p
    Anti Cd47, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd47/product/Bio X Cell
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd47 - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    93
    Abcam anti cd47
    Model of the SRSF10-regulated mIL1RAP-NF-κB axis upregulating the “don’t eat me” signal <t>CD47</t> in HPV16/18-positive CC
    Anti Cd47, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd47/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd47 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier


    N/A
    Mouse monoclonal to CD47 DC46 reacts with CD47 gp42 a 45 55 kDa molecule expressed on broad tissue and cells including hemopoietic cells epithelial endothelial cells and other tissue cells
      Buy from Supplier

    N/A
    This antibody reacts with the Ig domain of CD47 protein It has been shown to inhibit polymorphonuclear neutrophil PMN transmigration across cell monolayers and matrix CD47 originally named integrin associated
      Buy from Supplier

    N/A
    CD47 Antibody is a Rabbit Polyclonal against CD47
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    Image Search Results


    The structure of CD47/IAP.

    Journal: OncoTargets and therapy

    Article Title: Checkpoint CD47 Function On Tumor Metastasis And Immune Therapy

    doi: 10.2147/OTT.S220196

    Figure Lengend Snippet: The structure of CD47/IAP.

    Article Snippet: Interestingly, CD47 binds to SIRP-α, SIRP-α is the specific receptor on macrophages and T cell surface and CD47 is the only upregulated protein and gene in CTCs compared with primary tumors, indicating that CD47 has the survival advantage in peripheral blood CTCs.

    Techniques:

    The relative pathway of CD47.

    Journal: OncoTargets and therapy

    Article Title: Checkpoint CD47 Function On Tumor Metastasis And Immune Therapy

    doi: 10.2147/OTT.S220196

    Figure Lengend Snippet: The relative pathway of CD47.

    Article Snippet: Interestingly, CD47 binds to SIRP-α, SIRP-α is the specific receptor on macrophages and T cell surface and CD47 is the only upregulated protein and gene in CTCs compared with primary tumors, indicating that CD47 has the survival advantage in peripheral blood CTCs.

    Techniques:

    The function of CD47 in tumors and exosome.

    Journal: OncoTargets and therapy

    Article Title: Checkpoint CD47 Function On Tumor Metastasis And Immune Therapy

    doi: 10.2147/OTT.S220196

    Figure Lengend Snippet: The function of CD47 in tumors and exosome.

    Article Snippet: Interestingly, CD47 binds to SIRP-α, SIRP-α is the specific receptor on macrophages and T cell surface and CD47 is the only upregulated protein and gene in CTCs compared with primary tumors, indicating that CD47 has the survival advantage in peripheral blood CTCs.

    Techniques:

    Administration of RvD1 to WD-fed Ldlr −/− mice with established atherosclerosis decreases lesional CD47 and necrosis. Ldlr −/− mice were fed a WD for 12 weeks, after which Veh or 100 ng/mouse of RvD1 (3x/week i.p.) was administered for 3 weeks. Aortic root lesions were immunostained with anti-CD47 ( a ) or anti-p-SHP1 ( b ) antibody and counterstained with Hoechst. The mean fluorescence intensity of CD47 was quantified. c Lesional necrosis was quantified with Olympus DP2-BSW software. All results are mean ± sem, n = 8 separate mice per group. * p

    Journal: Cell Death and Differentiation

    Article Title: Resolvin D1 promotes the targeting and clearance of necroptotic cells

    doi: 10.1038/s41418-019-0370-1

    Figure Lengend Snippet: Administration of RvD1 to WD-fed Ldlr −/− mice with established atherosclerosis decreases lesional CD47 and necrosis. Ldlr −/− mice were fed a WD for 12 weeks, after which Veh or 100 ng/mouse of RvD1 (3x/week i.p.) was administered for 3 weeks. Aortic root lesions were immunostained with anti-CD47 ( a ) or anti-p-SHP1 ( b ) antibody and counterstained with Hoechst. The mean fluorescence intensity of CD47 was quantified. c Lesional necrosis was quantified with Olympus DP2-BSW software. All results are mean ± sem, n = 8 separate mice per group. * p

    Article Snippet: The remaining mice were injected with 100 μg/mouse of IgG or anti-CD47 (BioXCell, cat# MOPC-21 or MIAP410, respectively) for 3 weeks (3×/week, i.p.) while still on the WD.

    Techniques: Mouse Assay, Fluorescence, Software

    Overall scheme. NCs have high levels of CD47 that are clustered on the cell surface. Macrophages nibble the NCs in regions devoid of CD47 in a RhoA dependent manner. The uptake of NC enhances the biosynthesis of SPM. SPM, like RvD1 then enhances the engulfment of NCs in a CDC42 dependent manner. Additionally, RvD1 stimulates the release of ER proteins like calreticulin to target and label the NCs for swift engulfment in a CDC42 dependent manner

    Journal: Cell Death and Differentiation

    Article Title: Resolvin D1 promotes the targeting and clearance of necroptotic cells

    doi: 10.1038/s41418-019-0370-1

    Figure Lengend Snippet: Overall scheme. NCs have high levels of CD47 that are clustered on the cell surface. Macrophages nibble the NCs in regions devoid of CD47 in a RhoA dependent manner. The uptake of NC enhances the biosynthesis of SPM. SPM, like RvD1 then enhances the engulfment of NCs in a CDC42 dependent manner. Additionally, RvD1 stimulates the release of ER proteins like calreticulin to target and label the NCs for swift engulfment in a CDC42 dependent manner

    Article Snippet: The remaining mice were injected with 100 μg/mouse of IgG or anti-CD47 (BioXCell, cat# MOPC-21 or MIAP410, respectively) for 3 weeks (3×/week, i.p.) while still on the WD.

    Techniques:

    Administration of IgG or anti-CD47 to WD-fed Ldlr −/− mice with established atherosclerosis decreases lesional necroptotic cells and increases SPM in plaques. a Aortic roots lesion sections from Ldlr −/− mice were immunostained with an anti-p-MLKL antibody and Hoechst and the percent of p-MLKL + cells was quantified. b Aortic root lesions were stained with H E and necrotic areas were quantified with Olympus DP2-BSW software. Necrotic core is abbreviated as NC. Baseline indicates mice that were sacrificed after 10 weeks of WD feeding. Results are mean ± sem, n = 5 for baseline, n = 6 for IgG, and n = 7 for anti-CD47 separate mice per group. * p

    Journal: Cell Death and Differentiation

    Article Title: Resolvin D1 promotes the targeting and clearance of necroptotic cells

    doi: 10.1038/s41418-019-0370-1

    Figure Lengend Snippet: Administration of IgG or anti-CD47 to WD-fed Ldlr −/− mice with established atherosclerosis decreases lesional necroptotic cells and increases SPM in plaques. a Aortic roots lesion sections from Ldlr −/− mice were immunostained with an anti-p-MLKL antibody and Hoechst and the percent of p-MLKL + cells was quantified. b Aortic root lesions were stained with H E and necrotic areas were quantified with Olympus DP2-BSW software. Necrotic core is abbreviated as NC. Baseline indicates mice that were sacrificed after 10 weeks of WD feeding. Results are mean ± sem, n = 5 for baseline, n = 6 for IgG, and n = 7 for anti-CD47 separate mice per group. * p

    Article Snippet: The remaining mice were injected with 100 μg/mouse of IgG or anti-CD47 (BioXCell, cat# MOPC-21 or MIAP410, respectively) for 3 weeks (3×/week, i.p.) while still on the WD.

    Techniques: Mouse Assay, Staining, Software

    Model of the SRSF10-regulated mIL1RAP-NF-κB axis upregulating the “don’t eat me” signal CD47 in HPV16/18-positive CC

    Journal: Oncogene

    Article Title: SRSF10-mediated IL1RAP alternative splicing regulates cervical cancer oncogenesis via mIL1RAP-NF-κB-CD47 axis

    doi: 10.1038/s41388-017-0119-6

    Figure Lengend Snippet: Model of the SRSF10-regulated mIL1RAP-NF-κB axis upregulating the “don’t eat me” signal CD47 in HPV16/18-positive CC

    Article Snippet: Anti-SRSF10 (1:200, GTX47232, GeneTex, San Antonio, USA) or anti-CD47 (1:100, ab175388, Abcam) antibodies were used.

    Techniques:

    The effects of CD47 knockdown on the expression of PD-L1 signaling-related proteins in thyroid cancer cell lines. Protein expression levels of PD-L1, SHP-1, p-SHP-1, SHP-2, p-SHP-2, ERK, p-ERK, AKT, and p-AKT of thyroid cancer cells that underwent CD47 knockdown were evaluated by Western blot analysis. * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway

    doi:

    Figure Lengend Snippet: The effects of CD47 knockdown on the expression of PD-L1 signaling-related proteins in thyroid cancer cell lines. Protein expression levels of PD-L1, SHP-1, p-SHP-1, SHP-2, p-SHP-2, ERK, p-ERK, AKT, and p-AKT of thyroid cancer cells that underwent CD47 knockdown were evaluated by Western blot analysis. * P

    Article Snippet: The membranes were blocked with 5% low fat dried milk for 2 h, then incubated overnight at 4°C with corresponding primary antibodies directed against CD47 (Rabbit, 1:2000, ab175388, Abcam); PD-L1 (Rabbit, 1:1000, ab213524, Abcam); AKT (Rabbit, 1:1000, ab38449, Abcam); p-AKT (1:500, ab38449, Abcam); SHP1 (Rabbit, 1:2000, ab124942, Abcam); p-SHP1 (1:1000, ab51171, Abcam); SHP2 (Rabbit, 1:2000, ab32083, Abcam); p-SHP2 (1:50000, ab62322, Abcam); ERK (Rabbit, 1:1000, ab17942, Abcam); and p-ERK (1:1000, ab131438, Abcam).

    Techniques: Expressing, Western Blot

    The expression of the CD47 protein and mRNA in thyroid cancer cell lines. A. The protein expression of CD47 in normal Nthy-ori-3-1 human thyroid follicular epithelial cells and TPC-1 and K1 thyroid cancer (TC) cell lines was evaluated by Western blot analysis. B. Relative mRNA expression of CD47 in Nthy-ori-3-1, TPC-1, and K1cells was determined by qRT-PCR analysis. C. Flow cytometry analysis was performed to identify CD47 positive Nthy-ori-3-1, TPC-1 ad K1 cells. * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway

    doi:

    Figure Lengend Snippet: The expression of the CD47 protein and mRNA in thyroid cancer cell lines. A. The protein expression of CD47 in normal Nthy-ori-3-1 human thyroid follicular epithelial cells and TPC-1 and K1 thyroid cancer (TC) cell lines was evaluated by Western blot analysis. B. Relative mRNA expression of CD47 in Nthy-ori-3-1, TPC-1, and K1cells was determined by qRT-PCR analysis. C. Flow cytometry analysis was performed to identify CD47 positive Nthy-ori-3-1, TPC-1 ad K1 cells. * P

    Article Snippet: The membranes were blocked with 5% low fat dried milk for 2 h, then incubated overnight at 4°C with corresponding primary antibodies directed against CD47 (Rabbit, 1:2000, ab175388, Abcam); PD-L1 (Rabbit, 1:1000, ab213524, Abcam); AKT (Rabbit, 1:1000, ab38449, Abcam); p-AKT (1:500, ab38449, Abcam); SHP1 (Rabbit, 1:2000, ab124942, Abcam); p-SHP1 (1:1000, ab51171, Abcam); SHP2 (Rabbit, 1:2000, ab32083, Abcam); p-SHP2 (1:50000, ab62322, Abcam); ERK (Rabbit, 1:1000, ab17942, Abcam); and p-ERK (1:1000, ab131438, Abcam).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Flow Cytometry, Cytometry

    Co-immunoprecipitation for PD-L1 and CD47. PD-L1 and CD47 co-immunoprecipitated in the thyroid cells but not in the Nthy-ori-3-1 cells.

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway

    doi:

    Figure Lengend Snippet: Co-immunoprecipitation for PD-L1 and CD47. PD-L1 and CD47 co-immunoprecipitated in the thyroid cells but not in the Nthy-ori-3-1 cells.

    Article Snippet: The membranes were blocked with 5% low fat dried milk for 2 h, then incubated overnight at 4°C with corresponding primary antibodies directed against CD47 (Rabbit, 1:2000, ab175388, Abcam); PD-L1 (Rabbit, 1:1000, ab213524, Abcam); AKT (Rabbit, 1:1000, ab38449, Abcam); p-AKT (1:500, ab38449, Abcam); SHP1 (Rabbit, 1:2000, ab124942, Abcam); p-SHP1 (1:1000, ab51171, Abcam); SHP2 (Rabbit, 1:2000, ab32083, Abcam); p-SHP2 (1:50000, ab62322, Abcam); ERK (Rabbit, 1:1000, ab17942, Abcam); and p-ERK (1:1000, ab131438, Abcam).

    Techniques: Immunoprecipitation

    Effects of PD-L1 knockdown on cell apoptosis and cell cycle in thyroid cancer cells. A. The cell apoptosis of thyroid cancer (TC) cells using CD47 knockdown was evaluated by Annexin V/PI double staining, followed by a flow cytometry analysis. B. The cell cycle of TC cells that underwent CD47 knockdown was assessed by flow cytometry analysis. * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway

    doi:

    Figure Lengend Snippet: Effects of PD-L1 knockdown on cell apoptosis and cell cycle in thyroid cancer cells. A. The cell apoptosis of thyroid cancer (TC) cells using CD47 knockdown was evaluated by Annexin V/PI double staining, followed by a flow cytometry analysis. B. The cell cycle of TC cells that underwent CD47 knockdown was assessed by flow cytometry analysis. * P

    Article Snippet: The membranes were blocked with 5% low fat dried milk for 2 h, then incubated overnight at 4°C with corresponding primary antibodies directed against CD47 (Rabbit, 1:2000, ab175388, Abcam); PD-L1 (Rabbit, 1:1000, ab213524, Abcam); AKT (Rabbit, 1:1000, ab38449, Abcam); p-AKT (1:500, ab38449, Abcam); SHP1 (Rabbit, 1:2000, ab124942, Abcam); p-SHP1 (1:1000, ab51171, Abcam); SHP2 (Rabbit, 1:2000, ab32083, Abcam); p-SHP2 (1:50000, ab62322, Abcam); ERK (Rabbit, 1:1000, ab17942, Abcam); and p-ERK (1:1000, ab131438, Abcam).

    Techniques: Double Staining, Flow Cytometry, Cytometry

    The effects of CD47 knockdown on cell apoptosis and cell cycle in thyroid cancer cells. A. The cell apoptosis of thyroid cancer (TC) cells using CD47 knockdown was evaluated by Annexin V/PI double staining, followed by flow cytometry analysis. B. The cell cycle of the CD47 knocked down TC cells was assessed by flow cytometry analysis. * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway

    doi:

    Figure Lengend Snippet: The effects of CD47 knockdown on cell apoptosis and cell cycle in thyroid cancer cells. A. The cell apoptosis of thyroid cancer (TC) cells using CD47 knockdown was evaluated by Annexin V/PI double staining, followed by flow cytometry analysis. B. The cell cycle of the CD47 knocked down TC cells was assessed by flow cytometry analysis. * P

    Article Snippet: The membranes were blocked with 5% low fat dried milk for 2 h, then incubated overnight at 4°C with corresponding primary antibodies directed against CD47 (Rabbit, 1:2000, ab175388, Abcam); PD-L1 (Rabbit, 1:1000, ab213524, Abcam); AKT (Rabbit, 1:1000, ab38449, Abcam); p-AKT (1:500, ab38449, Abcam); SHP1 (Rabbit, 1:2000, ab124942, Abcam); p-SHP1 (1:1000, ab51171, Abcam); SHP2 (Rabbit, 1:2000, ab32083, Abcam); p-SHP2 (1:50000, ab62322, Abcam); ERK (Rabbit, 1:1000, ab17942, Abcam); and p-ERK (1:1000, ab131438, Abcam).

    Techniques: Double Staining, Flow Cytometry, Cytometry

    The expression of CD47 in pathological thyroid cancer tissue and healthy thyroid tissue. A. CD47 expression in healthy thyroid tissues, ×10. B. CD47 expression in healthy thyroid tissues, ×100. C. CD47 expression in TC pathological tissues, ×10. D. CD47 expression in TC pathological tissues, ×100. E. CD47 expression was assessed by Image-Pro Plus 6.0. * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway

    doi:

    Figure Lengend Snippet: The expression of CD47 in pathological thyroid cancer tissue and healthy thyroid tissue. A. CD47 expression in healthy thyroid tissues, ×10. B. CD47 expression in healthy thyroid tissues, ×100. C. CD47 expression in TC pathological tissues, ×10. D. CD47 expression in TC pathological tissues, ×100. E. CD47 expression was assessed by Image-Pro Plus 6.0. * P

    Article Snippet: The membranes were blocked with 5% low fat dried milk for 2 h, then incubated overnight at 4°C with corresponding primary antibodies directed against CD47 (Rabbit, 1:2000, ab175388, Abcam); PD-L1 (Rabbit, 1:1000, ab213524, Abcam); AKT (Rabbit, 1:1000, ab38449, Abcam); p-AKT (1:500, ab38449, Abcam); SHP1 (Rabbit, 1:2000, ab124942, Abcam); p-SHP1 (1:1000, ab51171, Abcam); SHP2 (Rabbit, 1:2000, ab32083, Abcam); p-SHP2 (1:50000, ab62322, Abcam); ERK (Rabbit, 1:1000, ab17942, Abcam); and p-ERK (1:1000, ab131438, Abcam).

    Techniques: Expressing