CD44 Antibody Search Results


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  • 99
    Thermo Fisher cd44
    Cd44, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd44/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd44 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    86
    biolegend cd44
    Identifying Neurl3 as a signature gene of HSC-primed HECs validated by functional and transcriptomic evaluation. (a) Dot plot showing the average and percentage expression of HEC signature genes in the indicated clusters. Genes are ordered by their median expression level in tif-HEC. Pre-HSC si gnature genes are marked as aquamarine. (b) Schematic model of the gene targeting strategy for generating Neurl3 EGFP/+ reporter mouse line via CRISPR/Cas9 system. (c) Representative FACS analysis of the E10.0 AGM region in Neurl3 EGFP/+ embryos, FACS plot to the right showing PK44 cells (red dots) mapped on it. (d) Representative FACS plot for sorting of the indicated cell populations from E10.0 caudal half of Neurl3 EGFP/+ embryos. (e) Graph showing the donor chimerism at 16 weeks after transplantation of the derivatives of the indicated populations from the caudal half of E10.0 Neurl3 EGFP/+ embryos. (f) Graph showing the donor chimerism at 4-16 weeks post-transplantation. The recipients were transplanted with the derivatives of CD41 - CD43 - CD45 - CD31 + <t>CD44</t> + Neurl3-EGFP + population from the caudal half of E10.0 Neurl3 EGFP/+ embryos. Number of repopulated/total recipients is shown in the brackets. (g) t-SNE plot of the cells included in the filtered initial dataset and additional PK44 and NE+ datasets, with clusters mapped on it. Cluster HEC and PK44 are combined as tif-HEC. NE+, CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from E10.0 AGM region. (h) Dot plot showing the average and percentage expression of selected HEC feature genes in the indicated clusters. Pre-HSC signature genes are marked as aquamarine. (i) Heatmap showing the correlation coefficient between each two clusters with hierarchical clustering using average method. Pearson correlation coefficient is calculated using average expression of highly variable genes in each cluster.
    Cd44, supplied by biolegend, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd44/product/biolegend
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd44 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    cd44  (Abcam)
    99
    Abcam cd44
    Identifying Neurl3 as a signature gene of HSC-primed HECs validated by functional and transcriptomic evaluation. (a) Dot plot showing the average and percentage expression of HEC signature genes in the indicated clusters. Genes are ordered by their median expression level in tif-HEC. Pre-HSC si gnature genes are marked as aquamarine. (b) Schematic model of the gene targeting strategy for generating Neurl3 EGFP/+ reporter mouse line via CRISPR/Cas9 system. (c) Representative FACS analysis of the E10.0 AGM region in Neurl3 EGFP/+ embryos, FACS plot to the right showing PK44 cells (red dots) mapped on it. (d) Representative FACS plot for sorting of the indicated cell populations from E10.0 caudal half of Neurl3 EGFP/+ embryos. (e) Graph showing the donor chimerism at 16 weeks after transplantation of the derivatives of the indicated populations from the caudal half of E10.0 Neurl3 EGFP/+ embryos. (f) Graph showing the donor chimerism at 4-16 weeks post-transplantation. The recipients were transplanted with the derivatives of CD41 - CD43 - CD45 - CD31 + <t>CD44</t> + Neurl3-EGFP + population from the caudal half of E10.0 Neurl3 EGFP/+ embryos. Number of repopulated/total recipients is shown in the brackets. (g) t-SNE plot of the cells included in the filtered initial dataset and additional PK44 and NE+ datasets, with clusters mapped on it. Cluster HEC and PK44 are combined as tif-HEC. NE+, CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from E10.0 AGM region. (h) Dot plot showing the average and percentage expression of selected HEC feature genes in the indicated clusters. Pre-HSC signature genes are marked as aquamarine. (i) Heatmap showing the correlation coefficient between each two clusters with hierarchical clustering using average method. Pearson correlation coefficient is calculated using average expression of highly variable genes in each cluster.
    Cd44, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd44/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd44 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    N/A
    Mouse Monoclonal Antibody Mab IgG2a K
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    N/A
    Rabbit anti CD44 Antibody Affinity Purified
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    N/A
    CD44 Antibody is a Rabbit Polyclonal antibody against CD44 The protein encoded by this gene is a cell surface glycoprotein involved in cell cell interactions cell adhesion and migration It
      Buy from Supplier


    Image Search Results


    Identifying Neurl3 as a signature gene of HSC-primed HECs validated by functional and transcriptomic evaluation. (a) Dot plot showing the average and percentage expression of HEC signature genes in the indicated clusters. Genes are ordered by their median expression level in tif-HEC. Pre-HSC si gnature genes are marked as aquamarine. (b) Schematic model of the gene targeting strategy for generating Neurl3 EGFP/+ reporter mouse line via CRISPR/Cas9 system. (c) Representative FACS analysis of the E10.0 AGM region in Neurl3 EGFP/+ embryos, FACS plot to the right showing PK44 cells (red dots) mapped on it. (d) Representative FACS plot for sorting of the indicated cell populations from E10.0 caudal half of Neurl3 EGFP/+ embryos. (e) Graph showing the donor chimerism at 16 weeks after transplantation of the derivatives of the indicated populations from the caudal half of E10.0 Neurl3 EGFP/+ embryos. (f) Graph showing the donor chimerism at 4-16 weeks post-transplantation. The recipients were transplanted with the derivatives of CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from the caudal half of E10.0 Neurl3 EGFP/+ embryos. Number of repopulated/total recipients is shown in the brackets. (g) t-SNE plot of the cells included in the filtered initial dataset and additional PK44 and NE+ datasets, with clusters mapped on it. Cluster HEC and PK44 are combined as tif-HEC. NE+, CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from E10.0 AGM region. (h) Dot plot showing the average and percentage expression of selected HEC feature genes in the indicated clusters. Pre-HSC signature genes are marked as aquamarine. (i) Heatmap showing the correlation coefficient between each two clusters with hierarchical clustering using average method. Pearson correlation coefficient is calculated using average expression of highly variable genes in each cluster.

    Journal: bioRxiv

    Article Title: Dissecting endothelial to haematopoietic stem cell transition by single-cell transcriptomic and functional analyses

    doi: 10.1101/2020.01.18.910356

    Figure Lengend Snippet: Identifying Neurl3 as a signature gene of HSC-primed HECs validated by functional and transcriptomic evaluation. (a) Dot plot showing the average and percentage expression of HEC signature genes in the indicated clusters. Genes are ordered by their median expression level in tif-HEC. Pre-HSC si gnature genes are marked as aquamarine. (b) Schematic model of the gene targeting strategy for generating Neurl3 EGFP/+ reporter mouse line via CRISPR/Cas9 system. (c) Representative FACS analysis of the E10.0 AGM region in Neurl3 EGFP/+ embryos, FACS plot to the right showing PK44 cells (red dots) mapped on it. (d) Representative FACS plot for sorting of the indicated cell populations from E10.0 caudal half of Neurl3 EGFP/+ embryos. (e) Graph showing the donor chimerism at 16 weeks after transplantation of the derivatives of the indicated populations from the caudal half of E10.0 Neurl3 EGFP/+ embryos. (f) Graph showing the donor chimerism at 4-16 weeks post-transplantation. The recipients were transplanted with the derivatives of CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from the caudal half of E10.0 Neurl3 EGFP/+ embryos. Number of repopulated/total recipients is shown in the brackets. (g) t-SNE plot of the cells included in the filtered initial dataset and additional PK44 and NE+ datasets, with clusters mapped on it. Cluster HEC and PK44 are combined as tif-HEC. NE+, CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from E10.0 AGM region. (h) Dot plot showing the average and percentage expression of selected HEC feature genes in the indicated clusters. Pre-HSC signature genes are marked as aquamarine. (i) Heatmap showing the correlation coefficient between each two clusters with hierarchical clustering using average method. Pearson correlation coefficient is calculated using average expression of highly variable genes in each cluster.

    Article Snippet: The primary antibodies were as follows: CD31 (BD Biosciences), CD44 (BD Biosciences), Endomucin (eBioscience), GFP (Cell Signaling), and Runx1 (Abcam).

    Techniques: Functional Assay, Expressing, CRISPR, FACS, Transplantation Assay

    In situ localization and in vitro function of the dynamic HECs marked by Neurl3-EGFP reporter. (a) Representative immunostaining on cross sections at AGM region of E9.5 (upper), E10.0 (middle) and E10.5 (lower) Neurl3 EGFP/+ embryos. Arrows indicate Neurl3 + aortic ECs. Yellow arrowheads indicate Neurl3 + bulging and bulged cells and IAHCs. Aquamarine arrowheads indicate CD44 + Runx1 + Neurl3 - haematopoietic cells distributed outside the aorta. nt, neural tube; DA, dorsal aorta. Scale bars, 100 μm. (b) Representative FACS analysis of the E10.0 AGM region of Neurl3 EGFP/+ embryos. FACS plots to the right showing PK44 cells (red dots, upper) and CD31 + Kit high cells (blue dots, lower) mapped on, respectively, with their contributions to each gated population indicated. (c) Representative CD31 and CD45 immunostaining on the cultures of single CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + cells from E10.0 AGM region of Neurl3 EGFP/+ embryos, showing typical morphologies regarding distinct differentiation potentials. Cell frequencies of each kind of potential are also shown. Data are from 5 independent experiments with totally 37 embryos used. Scale bars, 400 μm. (d) Column charts showing the proportions of positive wells in the indicated populations (lower) for each kind of potential. The experiments were performed with CD41 - CD43 - CD45 - CD31 + CD44 - Neurl3-EGFP + single cells from E9.5 caudal half or E10.0-E10.5 AGM region of Neurl3 EGFP/+ embryos with PK44 indexed. Progenies from PK44 and non-PK44 cells are represented by distinct fill patterns. (e) Expression of CD44 and Neurl3-EGFP and values of FSC-A and SSC-A in the index-sorted single CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + cells with differentiation potential based on in vitro functional evaluation. Cells with different kinds of potential are mapped onto the reference FACS plots (grey dots). Solid boxes (left of each stage) indicate the gates of the populations for FACS sorting. The enlarged views of solid boxes are shown below.

    Journal: bioRxiv

    Article Title: Dissecting endothelial to haematopoietic stem cell transition by single-cell transcriptomic and functional analyses

    doi: 10.1101/2020.01.18.910356

    Figure Lengend Snippet: In situ localization and in vitro function of the dynamic HECs marked by Neurl3-EGFP reporter. (a) Representative immunostaining on cross sections at AGM region of E9.5 (upper), E10.0 (middle) and E10.5 (lower) Neurl3 EGFP/+ embryos. Arrows indicate Neurl3 + aortic ECs. Yellow arrowheads indicate Neurl3 + bulging and bulged cells and IAHCs. Aquamarine arrowheads indicate CD44 + Runx1 + Neurl3 - haematopoietic cells distributed outside the aorta. nt, neural tube; DA, dorsal aorta. Scale bars, 100 μm. (b) Representative FACS analysis of the E10.0 AGM region of Neurl3 EGFP/+ embryos. FACS plots to the right showing PK44 cells (red dots, upper) and CD31 + Kit high cells (blue dots, lower) mapped on, respectively, with their contributions to each gated population indicated. (c) Representative CD31 and CD45 immunostaining on the cultures of single CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + cells from E10.0 AGM region of Neurl3 EGFP/+ embryos, showing typical morphologies regarding distinct differentiation potentials. Cell frequencies of each kind of potential are also shown. Data are from 5 independent experiments with totally 37 embryos used. Scale bars, 400 μm. (d) Column charts showing the proportions of positive wells in the indicated populations (lower) for each kind of potential. The experiments were performed with CD41 - CD43 - CD45 - CD31 + CD44 - Neurl3-EGFP + single cells from E9.5 caudal half or E10.0-E10.5 AGM region of Neurl3 EGFP/+ embryos with PK44 indexed. Progenies from PK44 and non-PK44 cells are represented by distinct fill patterns. (e) Expression of CD44 and Neurl3-EGFP and values of FSC-A and SSC-A in the index-sorted single CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + cells with differentiation potential based on in vitro functional evaluation. Cells with different kinds of potential are mapped onto the reference FACS plots (grey dots). Solid boxes (left of each stage) indicate the gates of the populations for FACS sorting. The enlarged views of solid boxes are shown below.

    Article Snippet: The primary antibodies were as follows: CD31 (BD Biosciences), CD44 (BD Biosciences), Endomucin (eBioscience), GFP (Cell Signaling), and Runx1 (Abcam).

    Techniques: In Situ, In Vitro, Immunostaining, FACS, Expressing, Functional Assay

    Efficiently isolating the HSC-competent and endothelial-haematopoietic bi-potent HECs before HSC emergence. (a) Gene lists of the top ten cell surface molecules significantly overrepresented in HEC as compared to the indicated cell populations (first 3 lines) and those positively correlated with Runx1 within 4 EC clusters (vEC, earlyAEC, lateAEC and HEC, last line). Non-HEC, cells except for HEC within 4 EC clusters. Highlights in red font indicate the candidates used for further functional analysis. (b) Representative whole-mount staining of CD44 at E10.0 AGM region, showing CD44 is expressed in the whole endothelial layer of the dorsal aorta and roots of its proximal branches. DA, dorsal aorta; Scale bar, 100 μm. (c) Representative FACS plots for cell sorting of the E9.5-E10.0 caudal half for co-culture/transplantation assay and the donor chimerism at 16 weeks after transplantation of the derivatives of the indicated cell populations. (d) Blood chimerism of the primary (I°) and corresponding secondary (II°) recipients at 16 weeks post-transplantation. The primary recipients were transplanted with the derivatives of the indicated cells from the caudal half of E9.5-E10.0 embryos. The paired primary and corresponding secondary repopulated mice are shown as the same symbol and color. (e) Bars represent the percent donor contribution to the granulocytes/monocytes (GM, red), B lymphocytes (green), and T lymphocytes (purple) in the peripheral blood of the primary (I°) and secondary (II°) recipients at 16 weeks post-transplantation. The paired primary and corresponding secondary repopulated mice are shown as the same colors below. (f) FACS plot of Flk1 expression in the indicated population of E10.0 AGM region, with PK44 (CD41 - CD43 - CD45 - CD31 + CD201 + Kit + CD44 + ) cells (red) mapped onto it. Red box indicates the gate of Flk1 + cells. (g) t-SNE plot of the cells included in the filtered initial dataset and PK44 dataset, with clusters mapped on it. PK44, CD41 - CD43 - CD45 - CD31 + CD201 + Kit + CD44 + population from E10.0 AGM region. (h) Heatmap showing the relative expressions of HEC feature genes, which are defined as those significantly highly expressed as compared to others including HC, vEC, earlyAEC and lateAEC, in the indicated cell populations. Selected HEC feature genes are shown to the right with pre-HSC signature genes marked as aquamarine. (i) Representative CD31 and CD45 immunostaining on the cultures of single PK44 cells from E10.0 AGM region, showing typical morphologies regarding distinct differentiation potentials. Cell frequencies of each kind of potential are also shown. Data are from 5 independent experiments with totally 15 embryos used. Scale bars, 400 μm. (j) Expression of Kit and CD201 in the index-sorted single PK44 cells with differentiation potential based on in vitro functional evaluation. Cells with different kinds of potential are mapped onto the reference FACS plots (grey dots). Box in the middle plot indicates the gate for FACS sorting of PK44 cells in E10.0 AGM region and its enlarged view is shown to the right.

    Journal: bioRxiv

    Article Title: Dissecting endothelial to haematopoietic stem cell transition by single-cell transcriptomic and functional analyses

    doi: 10.1101/2020.01.18.910356

    Figure Lengend Snippet: Efficiently isolating the HSC-competent and endothelial-haematopoietic bi-potent HECs before HSC emergence. (a) Gene lists of the top ten cell surface molecules significantly overrepresented in HEC as compared to the indicated cell populations (first 3 lines) and those positively correlated with Runx1 within 4 EC clusters (vEC, earlyAEC, lateAEC and HEC, last line). Non-HEC, cells except for HEC within 4 EC clusters. Highlights in red font indicate the candidates used for further functional analysis. (b) Representative whole-mount staining of CD44 at E10.0 AGM region, showing CD44 is expressed in the whole endothelial layer of the dorsal aorta and roots of its proximal branches. DA, dorsal aorta; Scale bar, 100 μm. (c) Representative FACS plots for cell sorting of the E9.5-E10.0 caudal half for co-culture/transplantation assay and the donor chimerism at 16 weeks after transplantation of the derivatives of the indicated cell populations. (d) Blood chimerism of the primary (I°) and corresponding secondary (II°) recipients at 16 weeks post-transplantation. The primary recipients were transplanted with the derivatives of the indicated cells from the caudal half of E9.5-E10.0 embryos. The paired primary and corresponding secondary repopulated mice are shown as the same symbol and color. (e) Bars represent the percent donor contribution to the granulocytes/monocytes (GM, red), B lymphocytes (green), and T lymphocytes (purple) in the peripheral blood of the primary (I°) and secondary (II°) recipients at 16 weeks post-transplantation. The paired primary and corresponding secondary repopulated mice are shown as the same colors below. (f) FACS plot of Flk1 expression in the indicated population of E10.0 AGM region, with PK44 (CD41 - CD43 - CD45 - CD31 + CD201 + Kit + CD44 + ) cells (red) mapped onto it. Red box indicates the gate of Flk1 + cells. (g) t-SNE plot of the cells included in the filtered initial dataset and PK44 dataset, with clusters mapped on it. PK44, CD41 - CD43 - CD45 - CD31 + CD201 + Kit + CD44 + population from E10.0 AGM region. (h) Heatmap showing the relative expressions of HEC feature genes, which are defined as those significantly highly expressed as compared to others including HC, vEC, earlyAEC and lateAEC, in the indicated cell populations. Selected HEC feature genes are shown to the right with pre-HSC signature genes marked as aquamarine. (i) Representative CD31 and CD45 immunostaining on the cultures of single PK44 cells from E10.0 AGM region, showing typical morphologies regarding distinct differentiation potentials. Cell frequencies of each kind of potential are also shown. Data are from 5 independent experiments with totally 15 embryos used. Scale bars, 400 μm. (j) Expression of Kit and CD201 in the index-sorted single PK44 cells with differentiation potential based on in vitro functional evaluation. Cells with different kinds of potential are mapped onto the reference FACS plots (grey dots). Box in the middle plot indicates the gate for FACS sorting of PK44 cells in E10.0 AGM region and its enlarged view is shown to the right.

    Article Snippet: The primary antibodies were as follows: CD31 (BD Biosciences), CD44 (BD Biosciences), Endomucin (eBioscience), GFP (Cell Signaling), and Runx1 (Abcam).

    Techniques: Functional Assay, Staining, FACS, Co-Culture Assay, Transplantation Assay, Mouse Assay, Expressing, Immunostaining, In Vitro

    Identification of the HSC-competent HECs marked by Neurl3-EGFP reporter. (a) Detailed information of the co-culture/transplantation assays performed with the caudal half cells from E10.0 Neurl3 EGFP/+ embryos. (b) Boxplot showing the transcriptional expression level of EGFP in NE+ cell population, NE+, CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from AGM region of E10.0 Neurl3 EGFP/+ embryos. (c) Scatter plots showing correlation of the expression of EGFP with that of Neurl3 and Runx1 , respectively. Fitted line and 95% confidence interval are shown in red. Pearson correlation coefficients and P values are also shown in blue text. (d) Stacked bar chart showing the constitution of different cell cycle phases in the indicated clusters. (e) Representative immunostaining on cross sections at AGM region of E11.0 Neurl3 EGFP/+ embryos. Arrow indicates Neurl3 + aortic ECs; Yellow arrowheads indicate Neurl3 + bulging and bulged cells and IAHCs. Aquamarine arrowheads indicate CD44 + Runx1 + Neurl3 - haematopoietic cells distributed outside the aorta. nt, neural tube; DA, dorsal aorta. Scale bars, 100 μm. (f) Detailed information of endothelial-haematopoietic bi-potential induction assays performed with cells from E9.5 caudal half and E10.0-E10.5 AGM region of Neurl3 EGFP/+ embryos.

    Journal: bioRxiv

    Article Title: Dissecting endothelial to haematopoietic stem cell transition by single-cell transcriptomic and functional analyses

    doi: 10.1101/2020.01.18.910356

    Figure Lengend Snippet: Identification of the HSC-competent HECs marked by Neurl3-EGFP reporter. (a) Detailed information of the co-culture/transplantation assays performed with the caudal half cells from E10.0 Neurl3 EGFP/+ embryos. (b) Boxplot showing the transcriptional expression level of EGFP in NE+ cell population, NE+, CD41 - CD43 - CD45 - CD31 + CD44 + Neurl3-EGFP + population from AGM region of E10.0 Neurl3 EGFP/+ embryos. (c) Scatter plots showing correlation of the expression of EGFP with that of Neurl3 and Runx1 , respectively. Fitted line and 95% confidence interval are shown in red. Pearson correlation coefficients and P values are also shown in blue text. (d) Stacked bar chart showing the constitution of different cell cycle phases in the indicated clusters. (e) Representative immunostaining on cross sections at AGM region of E11.0 Neurl3 EGFP/+ embryos. Arrow indicates Neurl3 + aortic ECs; Yellow arrowheads indicate Neurl3 + bulging and bulged cells and IAHCs. Aquamarine arrowheads indicate CD44 + Runx1 + Neurl3 - haematopoietic cells distributed outside the aorta. nt, neural tube; DA, dorsal aorta. Scale bars, 100 μm. (f) Detailed information of endothelial-haematopoietic bi-potential induction assays performed with cells from E9.5 caudal half and E10.0-E10.5 AGM region of Neurl3 EGFP/+ embryos.

    Article Snippet: The primary antibodies were as follows: CD31 (BD Biosciences), CD44 (BD Biosciences), Endomucin (eBioscience), GFP (Cell Signaling), and Runx1 (Abcam).

    Techniques: Co-Culture Assay, Transplantation Assay, Expressing, Immunostaining