CD3 Antibody Search Results


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  • 99
    Thermo Fisher anti cd3
    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with <t>anti-CD3/anti-CD28</t> under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.
    Anti Cd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd3/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd3 - by Bioz Stars, 2021-05
    99/100 stars
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    99
    BioLegend anti cd3
    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with <t>anti-CD3/anti-CD28</t> under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.
    Anti Cd3, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd3/product/BioLegend
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd3 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher cd3
    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with <t>anti-CD3/anti-CD28</t> under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.
    Cd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd3/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd3 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    86
    Agilent technologies cd3
    Distribution of CD4 + CD25 + CD27 + regulatory T cells in lymph nodes and synovial tissues. (A–C) Serial sections of a reactive lymph node stained with antibodies to CD4 (A), CD25 (B), and CD27 (C). (D–H) Serial sections of synovial biopsy from a JIA patient showing a T and B lymphoid aggregate stained with antibodies to <t>CD3</t> (D), CD20 (E), CD27 (F), CD25 (G), and CD4 (H). (I) A consecutive section stained with CD25 (red) and CD27 (green) by two-color fluorescence.
    Cd3, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd3/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd3 - by Bioz Stars, 2021-05
    86/100 stars
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    N/A
    Mouse Monoclonal Antibody Mab IgG2a
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    N/A
    CD3 is a component of T cell surface receptor TCR it cantains 5 invariable chains gamma delta epsilon and zeta eta Clevers et al 1988 showed the TCR CD3 complex
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    N/A
    Mouse monoclonal CD3 antibody
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    N/A
    CD3 is expressed typically at high levels on peripheral T cells and majority of T cell neoplasms Thymocytes express CD3 at different level on the cell surface in the course
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    Image Search Results


    IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28 under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.

    Journal: Biology Open

    Article Title: IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model

    doi: 10.1242/bio.036244

    Figure Lengend Snippet: IL-31R signaling did not influence the differentiation of CD4 + T cell sub-populations. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28 under Th1-, Th2- or Th17- polarizing conditions. Cells were assayed for the transcription factors T-bet, GATA-3, ROR-γt and cytokines IFN-γ, IL-4, IL-17 by flow cytometry. No difference on the differentiation of Th1(A), Th2 (B) and Th17 (C) was found between WT ( n =4) and IL-31RA KO mice ( n =4). All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated three times. P, polarization; NP, non-polarization.

    Article Snippet: T cells at 2 million cells/ml were washed and resuspended in 2 μM CFSE (Invitrogen) staining solution for 10 min, and CFSE fluorescence was measured by flow cytometry at 96 h after culture with 1 μl/ml anti-CD3 (CAT#85-16-0032-81)/anti-CD28 (CAT#85-16-0281-81) (both eBioscience).

    Techniques: Purification, Mouse Assay, Flow Cytometry, Cytometry, Two Tailed Test

    IL-31R signaling suppressed the proliferation of CD4 + T cells. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28. The activation ( n =4) (A) and proliferation ( n =4) (B) of CD4 + T cells were assayed by flow cytometry. All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated twice. Ctrl, control; Act, activation.

    Journal: Biology Open

    Article Title: IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model

    doi: 10.1242/bio.036244

    Figure Lengend Snippet: IL-31R signaling suppressed the proliferation of CD4 + T cells. Purified CD4 + T cells from WT and IL-31RA KO mice were stimulated with anti-CD3/anti-CD28. The activation ( n =4) (A) and proliferation ( n =4) (B) of CD4 + T cells were assayed by flow cytometry. All values are plotted and lines indicate means. Statistical significance was determined by two-tailed Student's t - tests. The experiment was repeated twice. Ctrl, control; Act, activation.

    Article Snippet: T cells at 2 million cells/ml were washed and resuspended in 2 μM CFSE (Invitrogen) staining solution for 10 min, and CFSE fluorescence was measured by flow cytometry at 96 h after culture with 1 μl/ml anti-CD3 (CAT#85-16-0032-81)/anti-CD28 (CAT#85-16-0281-81) (both eBioscience).

    Techniques: Purification, Mouse Assay, Activation Assay, Flow Cytometry, Cytometry, Two Tailed Test, Activated Clotting Time Assay

    Distribution of CD4 + CD25 + CD27 + regulatory T cells in lymph nodes and synovial tissues. (A–C) Serial sections of a reactive lymph node stained with antibodies to CD4 (A), CD25 (B), and CD27 (C). (D–H) Serial sections of synovial biopsy from a JIA patient showing a T and B lymphoid aggregate stained with antibodies to CD3 (D), CD20 (E), CD27 (F), CD25 (G), and CD4 (H). (I) A consecutive section stained with CD25 (red) and CD27 (green) by two-color fluorescence.

    Journal: The Journal of Experimental Medicine

    Article Title: Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia

    doi: 10.1084/jem.20050085

    Figure Lengend Snippet: Distribution of CD4 + CD25 + CD27 + regulatory T cells in lymph nodes and synovial tissues. (A–C) Serial sections of a reactive lymph node stained with antibodies to CD4 (A), CD25 (B), and CD27 (C). (D–H) Serial sections of synovial biopsy from a JIA patient showing a T and B lymphoid aggregate stained with antibodies to CD3 (D), CD20 (E), CD27 (F), CD25 (G), and CD4 (H). (I) A consecutive section stained with CD25 (red) and CD27 (green) by two-color fluorescence.

    Article Snippet: Paraffin serial sections were stained for 30 min at room temperature with mouse antibodies to CD4 (4B12), CD25 (25C04), CD27 (137B4; obtained from Neomarkers), CD20 (L26), and CD3 (polyclonal antisera; obtained from DakoCytomation) followed by anti–mouse Ig antibody conjugated to peroxidase-labeled dextran polymer (EnVision; DakoCytomation) and chromogenic diaminobenzidine substrate (DakoCytomation).

    Techniques: Staining, Fluorescence

    CD27 is stably expressed in proliferating regulatory T cells, whereas it is down-regulated on activated conventional T cells. (A) CD4 + CD25 + CD27 + regulatory T cells and CD4 + CD25 − CD27 + T cells (comprising naive and memory cells) were sorted from peripheral blood to > 99% purity. (B and C) Cells were labeled with CFSE and stimulated with plastic-bound CD3 antibodies in the absence or presence of IL-2 or CD28 antibodies. CD27 expression was measured as a function of cell division on days 5 (B) and 10 (C). One representative experiment out of three performed is shown.

    Journal: The Journal of Experimental Medicine

    Article Title: Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia

    doi: 10.1084/jem.20050085

    Figure Lengend Snippet: CD27 is stably expressed in proliferating regulatory T cells, whereas it is down-regulated on activated conventional T cells. (A) CD4 + CD25 + CD27 + regulatory T cells and CD4 + CD25 − CD27 + T cells (comprising naive and memory cells) were sorted from peripheral blood to > 99% purity. (B and C) Cells were labeled with CFSE and stimulated with plastic-bound CD3 antibodies in the absence or presence of IL-2 or CD28 antibodies. CD27 expression was measured as a function of cell division on days 5 (B) and 10 (C). One representative experiment out of three performed is shown.

    Article Snippet: Paraffin serial sections were stained for 30 min at room temperature with mouse antibodies to CD4 (4B12), CD25 (25C04), CD27 (137B4; obtained from Neomarkers), CD20 (L26), and CD3 (polyclonal antisera; obtained from DakoCytomation) followed by anti–mouse Ig antibody conjugated to peroxidase-labeled dextran polymer (EnVision; DakoCytomation) and chromogenic diaminobenzidine substrate (DakoCytomation).

    Techniques: Stable Transfection, Labeling, Expressing

    FoxP3 mRNA in CD4 + CD25 + T cell subsets in samples containing limiting cell numbers. CD4 + T cell subsets were sorted according to the expression of CD25 and CD27 and resorted, collecting 16 replicates of five cells each that were subsequently analyzed for expression of FoxP3 by PCR. Amplification of CD3 was used as control.

    Journal: The Journal of Experimental Medicine

    Article Title: Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia

    doi: 10.1084/jem.20050085

    Figure Lengend Snippet: FoxP3 mRNA in CD4 + CD25 + T cell subsets in samples containing limiting cell numbers. CD4 + T cell subsets were sorted according to the expression of CD25 and CD27 and resorted, collecting 16 replicates of five cells each that were subsequently analyzed for expression of FoxP3 by PCR. Amplification of CD3 was used as control.

    Article Snippet: Paraffin serial sections were stained for 30 min at room temperature with mouse antibodies to CD4 (4B12), CD25 (25C04), CD27 (137B4; obtained from Neomarkers), CD20 (L26), and CD3 (polyclonal antisera; obtained from DakoCytomation) followed by anti–mouse Ig antibody conjugated to peroxidase-labeled dextran polymer (EnVision; DakoCytomation) and chromogenic diaminobenzidine substrate (DakoCytomation).

    Techniques: Expressing, Polymerase Chain Reaction, Amplification

    CD27 identifies potent suppressor cells within CD4 + CD25 + synovial T cells. (A) Proliferation of CFSE-labeled CD4 + CD25 − peripheral blood T cells stimulated by anti-CD3 and DCs in the absence (dashed lines) or presence (solid line) of equal numbers of the indicated autologous CD4 + T cells isolated from synovial fluid. The percentage inhibition was calculated as in Fig. 1 . Comparable results were obtained using synovial T cells isolated from patient no. 14 (depicted) and nos. 2, 5, and 7. (B) Proliferation of CFSE-labeled CD4 + CD25 − peripheral blood T cells in the presence of serial twofold dilutions of autologous CD4 + CD25 + CD27 + T cells from peripheral blood (open circle) or synovial fluid (closed square). Shown is the total number of responder T cells that had undergone more than one cell division. Mean ± SD of triplicate cultures. Comparable results were obtained with samples from patient no. 15 (depicted) and nos. 5 and 7.

    Journal: The Journal of Experimental Medicine

    Article Title: Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia

    doi: 10.1084/jem.20050085

    Figure Lengend Snippet: CD27 identifies potent suppressor cells within CD4 + CD25 + synovial T cells. (A) Proliferation of CFSE-labeled CD4 + CD25 − peripheral blood T cells stimulated by anti-CD3 and DCs in the absence (dashed lines) or presence (solid line) of equal numbers of the indicated autologous CD4 + T cells isolated from synovial fluid. The percentage inhibition was calculated as in Fig. 1 . Comparable results were obtained using synovial T cells isolated from patient no. 14 (depicted) and nos. 2, 5, and 7. (B) Proliferation of CFSE-labeled CD4 + CD25 − peripheral blood T cells in the presence of serial twofold dilutions of autologous CD4 + CD25 + CD27 + T cells from peripheral blood (open circle) or synovial fluid (closed square). Shown is the total number of responder T cells that had undergone more than one cell division. Mean ± SD of triplicate cultures. Comparable results were obtained with samples from patient no. 15 (depicted) and nos. 5 and 7.

    Article Snippet: Paraffin serial sections were stained for 30 min at room temperature with mouse antibodies to CD4 (4B12), CD25 (25C04), CD27 (137B4; obtained from Neomarkers), CD20 (L26), and CD3 (polyclonal antisera; obtained from DakoCytomation) followed by anti–mouse Ig antibody conjugated to peroxidase-labeled dextran polymer (EnVision; DakoCytomation) and chromogenic diaminobenzidine substrate (DakoCytomation).

    Techniques: Labeling, Isolation, Inhibition

    CD4 + CD25 + T cells are enriched in synovial fluid of JIA patients, express high amounts of FoxP3 mRNA, and efficiently suppress proliferation of CD4 + CD25 − autologous T cells. (A) Mononuclear cells from peripheral blood (PBMC) and synovial fluid (SFMC) were obtained from the same JIA patient and stained with antibodies to CD4 and CD25. Shown is the percentage of CD25 + and CD25 − cells within the CD4 + population. (B) Percentages of CD4 + CD25 + in PBMCs and SFMCs in 15 JIA patients. p-value determined by Wilcoxon rank test. (C) PBMCs and SFMCs were sorted based on expression of CD4 and CD25, and analyzed for expression of FoxP3 mRNA relative to 18S rRNA by quantitative real-time PCR. 1 representative experiment out of 10 is shown. (D) Proliferation of 1.5 × 10 4 CFSE-labeled CD4 + CD25 − peripheral blood T cells stimulated by anti-CD3 and DCs in the presence of equal numbers of autologous CD4 + CD25 − T cells (unshaded histograms) or CD4 + CD25 + T cells (shaded histograms) isolated from PBMCs or SFMCs. The total number of responder T cells that had performed one or more cell divisions was determined by CFSE dilution analysis. Responder T cells proliferated to a similar extent in the absence of CD4 + CD25 − control T cells (not depicted). The percentage inhibition was calculated from the number of dividing responder cells in presence of CD4 + CD25 + T cells as compared with their number in presence of CD4 + CD25 − control cells. One representative experiment out of five is shown.

    Journal: The Journal of Experimental Medicine

    Article Title: Coexpression of CD25 and CD27 identifies FoxP3+ regulatory T cells in inflamed synovia

    doi: 10.1084/jem.20050085

    Figure Lengend Snippet: CD4 + CD25 + T cells are enriched in synovial fluid of JIA patients, express high amounts of FoxP3 mRNA, and efficiently suppress proliferation of CD4 + CD25 − autologous T cells. (A) Mononuclear cells from peripheral blood (PBMC) and synovial fluid (SFMC) were obtained from the same JIA patient and stained with antibodies to CD4 and CD25. Shown is the percentage of CD25 + and CD25 − cells within the CD4 + population. (B) Percentages of CD4 + CD25 + in PBMCs and SFMCs in 15 JIA patients. p-value determined by Wilcoxon rank test. (C) PBMCs and SFMCs were sorted based on expression of CD4 and CD25, and analyzed for expression of FoxP3 mRNA relative to 18S rRNA by quantitative real-time PCR. 1 representative experiment out of 10 is shown. (D) Proliferation of 1.5 × 10 4 CFSE-labeled CD4 + CD25 − peripheral blood T cells stimulated by anti-CD3 and DCs in the presence of equal numbers of autologous CD4 + CD25 − T cells (unshaded histograms) or CD4 + CD25 + T cells (shaded histograms) isolated from PBMCs or SFMCs. The total number of responder T cells that had performed one or more cell divisions was determined by CFSE dilution analysis. Responder T cells proliferated to a similar extent in the absence of CD4 + CD25 − control T cells (not depicted). The percentage inhibition was calculated from the number of dividing responder cells in presence of CD4 + CD25 + T cells as compared with their number in presence of CD4 + CD25 − control cells. One representative experiment out of five is shown.

    Article Snippet: Paraffin serial sections were stained for 30 min at room temperature with mouse antibodies to CD4 (4B12), CD25 (25C04), CD27 (137B4; obtained from Neomarkers), CD20 (L26), and CD3 (polyclonal antisera; obtained from DakoCytomation) followed by anti–mouse Ig antibody conjugated to peroxidase-labeled dextran polymer (EnVision; DakoCytomation) and chromogenic diaminobenzidine substrate (DakoCytomation).

    Techniques: Staining, Expressing, Real-time Polymerase Chain Reaction, Labeling, Isolation, Inhibition