CCL-21 Search Results


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  • 99
    ATCC hela 229 cells
    Hela 229 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hela 229 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
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    86
    Thermo Fisher gene exp ccl21 hs00171076 m1
    Gene Exp Ccl21 Hs00171076 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ccl21 hs00171076 m1/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
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    gene exp ccl21 hs00171076 m1 - by Bioz Stars, 2024-02
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    93
    R&D Systems ccl21
    Expression of <t>CCL21</t> and CCL19 in the spleen was reduced following chemotherapy. (A) Spleen cryostat sections from control mice or test mice (treated with Cis, Gem or 5-FU) were stained for CCL21 (red) and fibroblast antibodies (ER-TR7; green), which were imaged by confocal microscopy. Scale bar, 200 µm. Expression of (B) CCL21 and (C) CCL19 in the spleens of control and test mice in tissue homogenates was further detected by ELISA. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. *P<0.05, **P<0.01, ***P<0.001. n.s., not significant. Con, control; Cis, cisplatin; Gem, gemcitabine; 5-FU, fluorouracil, CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19.
    Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ccl21/product/R&D Systems
    Average 93 stars, based on 1 article reviews
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    ccl21 - by Bioz Stars, 2024-02
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    93
    R&D Systems human slc protein
    Expression of <t>CCL21</t> and CCL19 in the spleen was reduced following chemotherapy. (A) Spleen cryostat sections from control mice or test mice (treated with Cis, Gem or 5-FU) were stained for CCL21 (red) and fibroblast antibodies (ER-TR7; green), which were imaged by confocal microscopy. Scale bar, 200 µm. Expression of (B) CCL21 and (C) CCL19 in the spleens of control and test mice in tissue homogenates was further detected by ELISA. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. *P<0.05, **P<0.01, ***P<0.001. n.s., not significant. Con, control; Cis, cisplatin; Gem, gemcitabine; 5-FU, fluorouracil, CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19.
    Human Slc Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human slc protein/product/R&D Systems
    Average 93 stars, based on 1 article reviews
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    93
    R&D Systems mouse ccl21 6ckine duoset
    Viral but not bacterial infections suppress T cells responding to coinfections in spite of decreased <t>CCL21.</t> (A) Schematic of experimental design. Number of OVA-specific CD8+ T cells (B) and LLO190-specific CD4+ T cells (C) per spleen at the indicated time points. LOD = limit of detection. Each symbol in the day 14 plot is from one mouse. Data (mean+S.E.M.) from 5–6 are cumulative results from 2-independent experiments. Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test (* p<0.05, ** p<0.01, *** p<0.001.) (D) Schematic of experimental design. Number of NP396-, GP33- and GP276-specific CD8+ T cells (E) and GP61-specific CD4+ T cells (F) per spleen at the indicated time points. Data (mean+S.E.M.) from 4–6 mice are cumulative results from 2-independent experiments. Data were analyzed by unpaired two-tailed t test (n.s. = not significant.) (G) Levels of <t>CCL21</t> in various groups of mice. Data from are cumulative results from 2-independent experiments; each symbol represents an individual mouse. Data were analyzed by one-way ANOVA followed by Dunnet’s multiple comparison test (**** p<0.0001.)
    Mouse Ccl21 6ckine Duoset, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse ccl21 6ckine duoset/product/R&D Systems
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    mouse ccl21 6ckine duoset - by Bioz Stars, 2024-02
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    Image Search Results


    Expression of CCL21 and CCL19 in the spleen was reduced following chemotherapy. (A) Spleen cryostat sections from control mice or test mice (treated with Cis, Gem or 5-FU) were stained for CCL21 (red) and fibroblast antibodies (ER-TR7; green), which were imaged by confocal microscopy. Scale bar, 200 µm. Expression of (B) CCL21 and (C) CCL19 in the spleens of control and test mice in tissue homogenates was further detected by ELISA. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. *P<0.05, **P<0.01, ***P<0.001. n.s., not significant. Con, control; Cis, cisplatin; Gem, gemcitabine; 5-FU, fluorouracil, CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19.

    Journal: Oncology Letters

    Article Title: Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19

    doi: 10.3892/ol.2018.9287

    Figure Lengend Snippet: Expression of CCL21 and CCL19 in the spleen was reduced following chemotherapy. (A) Spleen cryostat sections from control mice or test mice (treated with Cis, Gem or 5-FU) were stained for CCL21 (red) and fibroblast antibodies (ER-TR7; green), which were imaged by confocal microscopy. Scale bar, 200 µm. Expression of (B) CCL21 and (C) CCL19 in the spleens of control and test mice in tissue homogenates was further detected by ELISA. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. *P<0.05, **P<0.01, ***P<0.001. n.s., not significant. Con, control; Cis, cisplatin; Gem, gemcitabine; 5-FU, fluorouracil, CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19.

    Article Snippet: The cryostat sections (10 µm) were fixed in ice-cold acetone for 10 min, prior to being incubated with the following antibodies at 4°C overnight: Anti-mouse ER-TR7 (cat. no. sc-73355; dilution, 1:100; Santa Cruz Biotechnology Inc., Dallas, TX, USA), and anti-mouse gp38 (cat. no. AF3244; dilution, 1:200; R&D Systems, Inc., Minneapolis, MN, USA); CCL21 (cat. no. AF457; dilution, 1:200; R&D Systems, Inc.).

    Techniques: Expressing, Staining, Confocal Microscopy, Enzyme-linked Immunosorbent Assay

    Chemotherapy does not affect the homing ability of T cells to the lymph nodes or chemokine expression in the lymph nodes. (A) The localization of CFSE + cells in the lymph nodes of control or test mice was ascertained 8 h after T cells transfer by immunofluorescence. Scale bar, 200 µm. (B) Counts of CFSE + cells in the in the lymph nodes of control or test mice. (C) Lymph node cryostat sections from control or test mice were stained for CCL21 (red) and fibroblast antibodies (ER-TR7; green). (D) Chemokine expression in the lymph nodes of control or test mice was quantified by reverse transcription-quantitative polymerase chain reaction. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. n.s., not significant. CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19; CFSE, carboxyfluorescein succinimidyl ester.

    Journal: Oncology Letters

    Article Title: Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19

    doi: 10.3892/ol.2018.9287

    Figure Lengend Snippet: Chemotherapy does not affect the homing ability of T cells to the lymph nodes or chemokine expression in the lymph nodes. (A) The localization of CFSE + cells in the lymph nodes of control or test mice was ascertained 8 h after T cells transfer by immunofluorescence. Scale bar, 200 µm. (B) Counts of CFSE + cells in the in the lymph nodes of control or test mice. (C) Lymph node cryostat sections from control or test mice were stained for CCL21 (red) and fibroblast antibodies (ER-TR7; green). (D) Chemokine expression in the lymph nodes of control or test mice was quantified by reverse transcription-quantitative polymerase chain reaction. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. n.s., not significant. CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19; CFSE, carboxyfluorescein succinimidyl ester.

    Article Snippet: The cryostat sections (10 µm) were fixed in ice-cold acetone for 10 min, prior to being incubated with the following antibodies at 4°C overnight: Anti-mouse ER-TR7 (cat. no. sc-73355; dilution, 1:100; Santa Cruz Biotechnology Inc., Dallas, TX, USA), and anti-mouse gp38 (cat. no. AF3244; dilution, 1:200; R&D Systems, Inc., Minneapolis, MN, USA); CCL21 (cat. no. AF457; dilution, 1:200; R&D Systems, Inc.).

    Techniques: Expressing, Immunofluorescence, Staining, Real-time Polymerase Chain Reaction

    Cytotoxic chemotherapy affects the function and survival of FRCs. (A) Spleen cryostat sections from control mice or chemotherapy-treated mice (Cis, Gem or 5-FU) were stained for TUNEL (red) and gp38 (green), which were imaged by confocal microscopy. Scale bar, 75 µm. (B) FRCs were co-cultured with Cis, Gem or 5-FU in vitro for 24 h, and then the expression in Annexin V + 7-AAD + cells were detected via flow cytometry. (C) The percentages of Annexin V + 7-AAD + FRCs in different groups. (D) Chemokine expression in the FRCs after 24 h of chemotherapeutic culture in vitro was quantified by reverse transcription-quantitative polymerase chain reaction. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. *P<0.05; **P<0.01; ***P<0.001; n.s., not significant. FRCs, fibroblastic reticular cells; Con, control; Cis, cisplatin; Gem, gemcitabine; 5-FU, fluorouracil CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19.

    Journal: Oncology Letters

    Article Title: Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19

    doi: 10.3892/ol.2018.9287

    Figure Lengend Snippet: Cytotoxic chemotherapy affects the function and survival of FRCs. (A) Spleen cryostat sections from control mice or chemotherapy-treated mice (Cis, Gem or 5-FU) were stained for TUNEL (red) and gp38 (green), which were imaged by confocal microscopy. Scale bar, 75 µm. (B) FRCs were co-cultured with Cis, Gem or 5-FU in vitro for 24 h, and then the expression in Annexin V + 7-AAD + cells were detected via flow cytometry. (C) The percentages of Annexin V + 7-AAD + FRCs in different groups. (D) Chemokine expression in the FRCs after 24 h of chemotherapeutic culture in vitro was quantified by reverse transcription-quantitative polymerase chain reaction. One-way analysis of variance, followed by Dunnett's multiple comparisons test, was used for all analyses. Error bars represent the standard error of the mean. *P<0.05; **P<0.01; ***P<0.001; n.s., not significant. FRCs, fibroblastic reticular cells; Con, control; Cis, cisplatin; Gem, gemcitabine; 5-FU, fluorouracil CCL21, C-C motif chemokine ligand 21; CCL19, C-C motif chemokine ligand 19.

    Article Snippet: The cryostat sections (10 µm) were fixed in ice-cold acetone for 10 min, prior to being incubated with the following antibodies at 4°C overnight: Anti-mouse ER-TR7 (cat. no. sc-73355; dilution, 1:100; Santa Cruz Biotechnology Inc., Dallas, TX, USA), and anti-mouse gp38 (cat. no. AF3244; dilution, 1:200; R&D Systems, Inc., Minneapolis, MN, USA); CCL21 (cat. no. AF457; dilution, 1:200; R&D Systems, Inc.).

    Techniques: Staining, TUNEL Assay, Confocal Microscopy, Cell Culture, In Vitro, Expressing, Flow Cytometry, Real-time Polymerase Chain Reaction

    Viral but not bacterial infections suppress T cells responding to coinfections in spite of decreased CCL21. (A) Schematic of experimental design. Number of OVA-specific CD8+ T cells (B) and LLO190-specific CD4+ T cells (C) per spleen at the indicated time points. LOD = limit of detection. Each symbol in the day 14 plot is from one mouse. Data (mean+S.E.M.) from 5–6 are cumulative results from 2-independent experiments. Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test (* p<0.05, ** p<0.01, *** p<0.001.) (D) Schematic of experimental design. Number of NP396-, GP33- and GP276-specific CD8+ T cells (E) and GP61-specific CD4+ T cells (F) per spleen at the indicated time points. Data (mean+S.E.M.) from 4–6 mice are cumulative results from 2-independent experiments. Data were analyzed by unpaired two-tailed t test (n.s. = not significant.) (G) Levels of CCL21 in various groups of mice. Data from are cumulative results from 2-independent experiments; each symbol represents an individual mouse. Data were analyzed by one-way ANOVA followed by Dunnet’s multiple comparison test (**** p<0.0001.)

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Plasmodium suppresses expansion of T cell responses to heterologous infections

    doi: 10.4049/jimmunol.1401745

    Figure Lengend Snippet: Viral but not bacterial infections suppress T cells responding to coinfections in spite of decreased CCL21. (A) Schematic of experimental design. Number of OVA-specific CD8+ T cells (B) and LLO190-specific CD4+ T cells (C) per spleen at the indicated time points. LOD = limit of detection. Each symbol in the day 14 plot is from one mouse. Data (mean+S.E.M.) from 5–6 are cumulative results from 2-independent experiments. Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test (* p<0.05, ** p<0.01, *** p<0.001.) (D) Schematic of experimental design. Number of NP396-, GP33- and GP276-specific CD8+ T cells (E) and GP61-specific CD4+ T cells (F) per spleen at the indicated time points. Data (mean+S.E.M.) from 4–6 mice are cumulative results from 2-independent experiments. Data were analyzed by unpaired two-tailed t test (n.s. = not significant.) (G) Levels of CCL21 in various groups of mice. Data from are cumulative results from 2-independent experiments; each symbol represents an individual mouse. Data were analyzed by one-way ANOVA followed by Dunnet’s multiple comparison test (**** p<0.0001.)

    Article Snippet: CCL21 was quantified using Mouse CCL21/6Ckine DuoSet (R&D Systems, Minneapolis, MN) according to the manufacturer’s recommendations.

    Techniques: Two Tailed Test