CCL-183 Search Results


95
ATCC d 17 ccl 183 cell line
D 17 Ccl 183 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC f5m2 osteosarcoma cell lines
Oct4 siRNA reduces Oct4 expression in <t>F5M2</t> cells. F5M2 cells were treated with Oct4 siRNA or scramble for 3 days. (A) mRNA and (B) protein expression levels were measured by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively (n=6). *P<0.05, **P<0.01 vs. the scramble group. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA.
F5m2 Osteosarcoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
LGC Promochem osteosarcoma cells ccl 183
Oct4 siRNA reduces Oct4 expression in <t>F5M2</t> cells. F5M2 cells were treated with Oct4 siRNA or scramble for 3 days. (A) mRNA and (B) protein expression levels were measured by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively (n=6). *P<0.05, **P<0.01 vs. the scramble group. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA.
Osteosarcoma Cells Ccl 183, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Oct4 siRNA reduces Oct4 expression in F5M2 cells. F5M2 cells were treated with Oct4 siRNA or scramble for 3 days. (A) mRNA and (B) protein expression levels were measured by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively (n=6). *P<0.05, **P<0.01 vs. the scramble group. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA.

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: Oct4 siRNA reduces Oct4 expression in F5M2 cells. F5M2 cells were treated with Oct4 siRNA or scramble for 3 days. (A) mRNA and (B) protein expression levels were measured by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively (n=6). *P<0.05, **P<0.01 vs. the scramble group. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, Small Interfering RNA

Oct4 downregulation decreases proliferation and invasion ability of F5M2 cells. F5M2 cells were treated with of Oct4 siRNA1 or scramble for 3 days. (A) Proliferation was measured by MTT assay, (B) cell apoptosis was assayed using Annexin V by flow cytometry, and (C) invasion ability was evaluated by Transwell assay. Magnification, ×200. (D) Quantified data of invasion has been presented (n=6). *P<0.01 vs. the control. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA; FITC, fluorescien isothiocyanate; PI, propidium iodide.

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: Oct4 downregulation decreases proliferation and invasion ability of F5M2 cells. F5M2 cells were treated with of Oct4 siRNA1 or scramble for 3 days. (A) Proliferation was measured by MTT assay, (B) cell apoptosis was assayed using Annexin V by flow cytometry, and (C) invasion ability was evaluated by Transwell assay. Magnification, ×200. (D) Quantified data of invasion has been presented (n=6). *P<0.01 vs. the control. Oct4, octamer-binding transcription factor 4; siRNA, small interfering RNA; FITC, fluorescien isothiocyanate; PI, propidium iodide.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: MTT Assay, Flow Cytometry, Transwell Assay, Binding Assay, Small Interfering RNA

Effects of Oct4 downregulation on the expression levels of numerous lncRNAs. F5M2 cells were treated with Oct4 siRNA1 or scramble for 3 days. The expressions of lncRNAs were measured by real-time RT-PCR (n=6). *P<0.01 vs. scramble. lncRNA, long non-coding RNA; siRNA, small interfering RNA; Oct4, octamer-binding transcription factor 4; BCAR4, breast cancer anti-estrogen resistance 4; CASC2, cancer susceptibility candidate 2; HIF1A-AS1, HIF1A antisense RNA 1; PCA3, prostate cancer associated 3; PVT1, Pvt1 oncogene; SNHG5, small nucleolar RNA host gene 5.

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: Effects of Oct4 downregulation on the expression levels of numerous lncRNAs. F5M2 cells were treated with Oct4 siRNA1 or scramble for 3 days. The expressions of lncRNAs were measured by real-time RT-PCR (n=6). *P<0.01 vs. scramble. lncRNA, long non-coding RNA; siRNA, small interfering RNA; Oct4, octamer-binding transcription factor 4; BCAR4, breast cancer anti-estrogen resistance 4; CASC2, cancer susceptibility candidate 2; HIF1A-AS1, HIF1A antisense RNA 1; PCA3, prostate cancer associated 3; PVT1, Pvt1 oncogene; SNHG5, small nucleolar RNA host gene 5.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Small Interfering RNA, Binding Assay

AK055347 downregulation decreases proliferation and invasion ability of F5M2 cells. F5M2 cells were treated with of AK055347 siRNA3 or scramble for 3 days. (A) Proliferation was measured by MTT assay, (B) cell apoptosis was assayed with Annexin V by flow cytometry and (C) invasion ability was evaluated by Transwell assay (n=6). *P<0.05, **P<0.01 vs. the scramble group. (D) AK055347 downregulation decreased cell invasion ability. Magnification, ×200. siRNA, small interfering RNA; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Journal: Oncology Letters

Article Title: Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

doi: 10.3892/ol.2016.5400

Figure Lengend Snippet: AK055347 downregulation decreases proliferation and invasion ability of F5M2 cells. F5M2 cells were treated with of AK055347 siRNA3 or scramble for 3 days. (A) Proliferation was measured by MTT assay, (B) cell apoptosis was assayed with Annexin V by flow cytometry and (C) invasion ability was evaluated by Transwell assay (n=6). *P<0.05, **P<0.01 vs. the scramble group. (D) AK055347 downregulation decreased cell invasion ability. Magnification, ×200. siRNA, small interfering RNA; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: SOSP-9607, MG63 and F5M2 osteosarcoma cell lines and the hFOB1.19 osteoblast cell line were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: MTT Assay, Flow Cytometry, Transwell Assay, Small Interfering RNA