C-28077 Search Results


spheroid formation  (PromoCell)
94
PromoCell spheroid formation
Spheroid Formation, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spheroid formation/product/PromoCell
Average 94 stars, based on 1 article reviews
spheroid formation - by Bioz Stars, 2025-11
94/100 stars
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uwb1 cell lines  (PromoCell)
93
PromoCell uwb1 cell lines
BRCA1-deficient <t>UWB1.289</t> cells corrected with wild-type BRCA1 gene (BRCA1+; closed triangle) and parental BRCA1-deficient UWB1.289 (BRCA1-; closed circle). Two PARPi-resistant cell lines derived from UWB1.289, UWB1.289.SYr12 (closed diamond) and UWB1.SYr13 (X), were also examined. Cell lines were treated with (A) quinacrine, (B) mitoxantrone, and (C) doxorubicin as described in .
Uwb1 Cell Lines, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uwb1 cell lines/product/PromoCell
Average 93 stars, based on 1 article reviews
uwb1 cell lines - by Bioz Stars, 2025-11
93/100 stars
  Buy from Supplier

Image Search Results


BRCA1-deficient UWB1.289 cells corrected with wild-type BRCA1 gene (BRCA1+; closed triangle) and parental BRCA1-deficient UWB1.289 (BRCA1-; closed circle). Two PARPi-resistant cell lines derived from UWB1.289, UWB1.289.SYr12 (closed diamond) and UWB1.SYr13 (X), were also examined. Cell lines were treated with (A) quinacrine, (B) mitoxantrone, and (C) doxorubicin as described in .

Journal: PLoS ONE

Article Title: Selective killing of homologous recombination-deficient cancer cell lines by inhibitors of the RPA:RAD52 protein-protein interaction

doi: 10.1371/journal.pone.0248941

Figure Lengend Snippet: BRCA1-deficient UWB1.289 cells corrected with wild-type BRCA1 gene (BRCA1+; closed triangle) and parental BRCA1-deficient UWB1.289 (BRCA1-; closed circle). Two PARPi-resistant cell lines derived from UWB1.289, UWB1.289.SYr12 (closed diamond) and UWB1.SYr13 (X), were also examined. Cell lines were treated with (A) quinacrine, (B) mitoxantrone, and (C) doxorubicin as described in .

Article Snippet: The Mammary Epithelial growth media, along with SupplementMix solution for UWB1 cell lines, were obtained from PromoCell GmbH and mixed as described [ , ].

Techniques: Derivative Assay

UWB1.289 cells with or without restoration of the BRCA1 expression were treated with 0.2 μM of mitoxantrone (MX) for three days. Olaparib (Ola) at 1 μM was used as control for the induction of apoptosis. Near EC 50 concentrations were selected for each compound. After the treatment with each compound, cell lysates were prepared and cleaved PARP was detected with (A) anti-cleaved PARP antibody (Cell Signaling Technology #9541) and (B) anti-PARP antibody (Cell Signaling Technology #9542). Tubulin detected with anti-beta-tubulin antibody (Cell Signaling Technology #15115) was used as an internal control. (C) Ratios of cleaved PARP to tubulin were determined with ImageJ and graphed. Three independent experiments were performed and the mean values are shown. The bars indicate standard deviations. Statistical significance of differences was determined with Student t-test (* p<0.05 and ** p<0.01).

Journal: PLoS ONE

Article Title: Selective killing of homologous recombination-deficient cancer cell lines by inhibitors of the RPA:RAD52 protein-protein interaction

doi: 10.1371/journal.pone.0248941

Figure Lengend Snippet: UWB1.289 cells with or without restoration of the BRCA1 expression were treated with 0.2 μM of mitoxantrone (MX) for three days. Olaparib (Ola) at 1 μM was used as control for the induction of apoptosis. Near EC 50 concentrations were selected for each compound. After the treatment with each compound, cell lysates were prepared and cleaved PARP was detected with (A) anti-cleaved PARP antibody (Cell Signaling Technology #9541) and (B) anti-PARP antibody (Cell Signaling Technology #9542). Tubulin detected with anti-beta-tubulin antibody (Cell Signaling Technology #15115) was used as an internal control. (C) Ratios of cleaved PARP to tubulin were determined with ImageJ and graphed. Three independent experiments were performed and the mean values are shown. The bars indicate standard deviations. Statistical significance of differences was determined with Student t-test (* p<0.05 and ** p<0.01).

Article Snippet: The Mammary Epithelial growth media, along with SupplementMix solution for UWB1 cell lines, were obtained from PromoCell GmbH and mixed as described [ , ].

Techniques: Expressing