C-14053 Search Results


human aortic smc haosmc stocks  (PromoCell)
95
PromoCell human aortic smc haosmc stocks
The effect of iSN04 on proliferation and differentiation of hAoSMCs. ( A ) Representative fluorescence images of nucleolin staining of hAoSMCs in hGM (day 0) and hDM (day 4). Scale bar, 50 μm. ( B ) Representative fluorescence images of EdU staining of hAoSMCs pre-treated with 30 μM iSN04 in hGM for 48 h and then with 10 μM EdU in hGM for 12 h. Scale bar, 200 μm. The ratio of EdU + cells were quantified. ** p < 0.01 vs. control (Student’s t -test). n = 4. ( C ) qPCR results of a cell-cycle marker gene, Ki-67 ( MKI67 ), and contractile <t>SMC</t> marker genes, α-SMA ( ACTA2 ), SM22α ( TAGLN ), and caldesmon ( CALD1 ), in hAoSMCs treated with 30 μM iSN04 in hGM for 48 h. * p < 0.05; ** p < 0.01 vs. control (Student’s t -test). n = 3. DAPI, 4′,6-diamidino-2-phenylindole; DM, differentiation medium; EdU, 5-ethynyl-2′-deoxyuridine; GM, growth medium; <t>hAoSMC,</t> human aortic smooth muscle cell; hDM, differentiation medium for human aortic smooth muscle cell; hGM, growth medium for human aortic smooth muscle cell; qPCR, quantitative real-time RT-PCR; SMC, smooth muscle cell.
Human Aortic Smc Haosmc Stocks, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human aortic smc haosmc stocks/product/PromoCell
Average 95 stars, based on 1 article reviews
human aortic smc haosmc stocks - by Bioz Stars, 2025-11
95/100 stars
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aortic smooth muscle cells  (PromoCell)
95
PromoCell aortic smooth muscle cells
The effect of iSN04 on proliferation and differentiation of hAoSMCs. ( A ) Representative fluorescence images of nucleolin staining of hAoSMCs in hGM (day 0) and hDM (day 4). Scale bar, 50 μm. ( B ) Representative fluorescence images of EdU staining of hAoSMCs pre-treated with 30 μM iSN04 in hGM for 48 h and then with 10 μM EdU in hGM for 12 h. Scale bar, 200 μm. The ratio of EdU + cells were quantified. ** p < 0.01 vs. control (Student’s t -test). n = 4. ( C ) qPCR results of a cell-cycle marker gene, Ki-67 ( MKI67 ), and contractile <t>SMC</t> marker genes, α-SMA ( ACTA2 ), SM22α ( TAGLN ), and caldesmon ( CALD1 ), in hAoSMCs treated with 30 μM iSN04 in hGM for 48 h. * p < 0.05; ** p < 0.01 vs. control (Student’s t -test). n = 3. DAPI, 4′,6-diamidino-2-phenylindole; DM, differentiation medium; EdU, 5-ethynyl-2′-deoxyuridine; GM, growth medium; <t>hAoSMC,</t> human aortic smooth muscle cell; hDM, differentiation medium for human aortic smooth muscle cell; hGM, growth medium for human aortic smooth muscle cell; qPCR, quantitative real-time RT-PCR; SMC, smooth muscle cell.
Aortic Smooth Muscle Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aortic smooth muscle cells/product/PromoCell
Average 95 stars, based on 1 article reviews
aortic smooth muscle cells - by Bioz Stars, 2025-11
95/100 stars
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human aortic smooth muscle cells huasmcs  (PromoCell)
95
PromoCell human aortic smooth muscle cells huasmcs
The effect of iSN04 on proliferation and differentiation of hAoSMCs. ( A ) Representative fluorescence images of nucleolin staining of hAoSMCs in hGM (day 0) and hDM (day 4). Scale bar, 50 μm. ( B ) Representative fluorescence images of EdU staining of hAoSMCs pre-treated with 30 μM iSN04 in hGM for 48 h and then with 10 μM EdU in hGM for 12 h. Scale bar, 200 μm. The ratio of EdU + cells were quantified. ** p < 0.01 vs. control (Student’s t -test). n = 4. ( C ) qPCR results of a cell-cycle marker gene, Ki-67 ( MKI67 ), and contractile <t>SMC</t> marker genes, α-SMA ( ACTA2 ), SM22α ( TAGLN ), and caldesmon ( CALD1 ), in hAoSMCs treated with 30 μM iSN04 in hGM for 48 h. * p < 0.05; ** p < 0.01 vs. control (Student’s t -test). n = 3. DAPI, 4′,6-diamidino-2-phenylindole; DM, differentiation medium; EdU, 5-ethynyl-2′-deoxyuridine; GM, growth medium; <t>hAoSMC,</t> human aortic smooth muscle cell; hDM, differentiation medium for human aortic smooth muscle cell; hGM, growth medium for human aortic smooth muscle cell; qPCR, quantitative real-time RT-PCR; SMC, smooth muscle cell.
Human Aortic Smooth Muscle Cells Huasmcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human aortic smooth muscle cells huasmcs/product/PromoCell
Average 95 stars, based on 1 article reviews
human aortic smooth muscle cells huasmcs - by Bioz Stars, 2025-11
95/100 stars
  Buy from Supplier
osteoblastic differentiation human arterial smooth muscle cells  (PromoCell)
95
PromoCell osteoblastic differentiation human arterial smooth muscle cells
The effect of iSN04 on proliferation and differentiation of hAoSMCs. ( A ) Representative fluorescence images of nucleolin staining of hAoSMCs in hGM (day 0) and hDM (day 4). Scale bar, 50 μm. ( B ) Representative fluorescence images of EdU staining of hAoSMCs pre-treated with 30 μM iSN04 in hGM for 48 h and then with 10 μM EdU in hGM for 12 h. Scale bar, 200 μm. The ratio of EdU + cells were quantified. ** p < 0.01 vs. control (Student’s t -test). n = 4. ( C ) qPCR results of a cell-cycle marker gene, Ki-67 ( MKI67 ), and contractile <t>SMC</t> marker genes, α-SMA ( ACTA2 ), SM22α ( TAGLN ), and caldesmon ( CALD1 ), in hAoSMCs treated with 30 μM iSN04 in hGM for 48 h. * p < 0.05; ** p < 0.01 vs. control (Student’s t -test). n = 3. DAPI, 4′,6-diamidino-2-phenylindole; DM, differentiation medium; EdU, 5-ethynyl-2′-deoxyuridine; GM, growth medium; <t>hAoSMC,</t> human aortic smooth muscle cell; hDM, differentiation medium for human aortic smooth muscle cell; hGM, growth medium for human aortic smooth muscle cell; qPCR, quantitative real-time RT-PCR; SMC, smooth muscle cell.
Osteoblastic Differentiation Human Arterial Smooth Muscle Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/osteoblastic differentiation human arterial smooth muscle cells/product/PromoCell
Average 95 stars, based on 1 article reviews
osteoblastic differentiation human arterial smooth muscle cells - by Bioz Stars, 2025-11
95/100 stars
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human airway smooth muscle cells hasmcs  (PromoCell)
95
PromoCell human airway smooth muscle cells hasmcs
The effect of iSN04 on proliferation and differentiation of hAoSMCs. ( A ) Representative fluorescence images of nucleolin staining of hAoSMCs in hGM (day 0) and hDM (day 4). Scale bar, 50 μm. ( B ) Representative fluorescence images of EdU staining of hAoSMCs pre-treated with 30 μM iSN04 in hGM for 48 h and then with 10 μM EdU in hGM for 12 h. Scale bar, 200 μm. The ratio of EdU + cells were quantified. ** p < 0.01 vs. control (Student’s t -test). n = 4. ( C ) qPCR results of a cell-cycle marker gene, Ki-67 ( MKI67 ), and contractile <t>SMC</t> marker genes, α-SMA ( ACTA2 ), SM22α ( TAGLN ), and caldesmon ( CALD1 ), in hAoSMCs treated with 30 μM iSN04 in hGM for 48 h. * p < 0.05; ** p < 0.01 vs. control (Student’s t -test). n = 3. DAPI, 4′,6-diamidino-2-phenylindole; DM, differentiation medium; EdU, 5-ethynyl-2′-deoxyuridine; GM, growth medium; <t>hAoSMC,</t> human aortic smooth muscle cell; hDM, differentiation medium for human aortic smooth muscle cell; hGM, growth medium for human aortic smooth muscle cell; qPCR, quantitative real-time RT-PCR; SMC, smooth muscle cell.
Human Airway Smooth Muscle Cells Hasmcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human airway smooth muscle cells hasmcs/product/PromoCell
Average 95 stars, based on 1 article reviews
human airway smooth muscle cells hasmcs - by Bioz Stars, 2025-11
95/100 stars
  Buy from Supplier

Image Search Results


The effect of iSN04 on proliferation and differentiation of hAoSMCs. ( A ) Representative fluorescence images of nucleolin staining of hAoSMCs in hGM (day 0) and hDM (day 4). Scale bar, 50 μm. ( B ) Representative fluorescence images of EdU staining of hAoSMCs pre-treated with 30 μM iSN04 in hGM for 48 h and then with 10 μM EdU in hGM for 12 h. Scale bar, 200 μm. The ratio of EdU + cells were quantified. ** p < 0.01 vs. control (Student’s t -test). n = 4. ( C ) qPCR results of a cell-cycle marker gene, Ki-67 ( MKI67 ), and contractile SMC marker genes, α-SMA ( ACTA2 ), SM22α ( TAGLN ), and caldesmon ( CALD1 ), in hAoSMCs treated with 30 μM iSN04 in hGM for 48 h. * p < 0.05; ** p < 0.01 vs. control (Student’s t -test). n = 3. DAPI, 4′,6-diamidino-2-phenylindole; DM, differentiation medium; EdU, 5-ethynyl-2′-deoxyuridine; GM, growth medium; hAoSMC, human aortic smooth muscle cell; hDM, differentiation medium for human aortic smooth muscle cell; hGM, growth medium for human aortic smooth muscle cell; qPCR, quantitative real-time RT-PCR; SMC, smooth muscle cell.

Journal: Biomolecules

Article Title: Myogenic Anti-Nucleolin Aptamer iSN04 Inhibits Proliferation and Promotes Differentiation of Vascular Smooth Muscle Cells

doi: 10.3390/biom14060709

Figure Lengend Snippet: The effect of iSN04 on proliferation and differentiation of hAoSMCs. ( A ) Representative fluorescence images of nucleolin staining of hAoSMCs in hGM (day 0) and hDM (day 4). Scale bar, 50 μm. ( B ) Representative fluorescence images of EdU staining of hAoSMCs pre-treated with 30 μM iSN04 in hGM for 48 h and then with 10 μM EdU in hGM for 12 h. Scale bar, 200 μm. The ratio of EdU + cells were quantified. ** p < 0.01 vs. control (Student’s t -test). n = 4. ( C ) qPCR results of a cell-cycle marker gene, Ki-67 ( MKI67 ), and contractile SMC marker genes, α-SMA ( ACTA2 ), SM22α ( TAGLN ), and caldesmon ( CALD1 ), in hAoSMCs treated with 30 μM iSN04 in hGM for 48 h. * p < 0.05; ** p < 0.01 vs. control (Student’s t -test). n = 3. DAPI, 4′,6-diamidino-2-phenylindole; DM, differentiation medium; EdU, 5-ethynyl-2′-deoxyuridine; GM, growth medium; hAoSMC, human aortic smooth muscle cell; hDM, differentiation medium for human aortic smooth muscle cell; hGM, growth medium for human aortic smooth muscle cell; qPCR, quantitative real-time RT-PCR; SMC, smooth muscle cell.

Article Snippet: The commercially available human aortic SMC (hAoSMC) stocks from a healthy 55-year-old male and a 51-year-old female (C-12533, lot 437Z012.2 and 437Z016.2, respectively; PromoCell, Heidelberg, Germany) were maintained using the Smooth Muscle Cell Growth Medium 2 Kit (C-22162; PromoCell) as GM for hAoSMC (hGM) and induced to differentiate in DM for hAoSMC (hDM), consisting of DMEM, 1% FBS, and P/S.

Techniques: Fluorescence, Staining, Control, Marker, Quantitative RT-PCR