C-12980 Search Results


placental pericytes  (PromoCell)
95
PromoCell placental pericytes
Mouse mural cells sub-cluster into five groups, resembling cell types previously described in the brain, with one (aaSMC) detected only day 5 after PSNL or sham surgery (A). Acta2 & Tagln high vascular smooth muscle cells (vSMC), arteriolar SMCs enriched for Slit3 and Ctnna3 (aaSMC), Vtn & Abcc9 & Kcnj8 positive <t>pericytes</t> (PC1&2), Adgrf5 negative (PC3) and Acta2 negative pericytes (PC-NC). Expression of marker genes is plotted in B. No prominent differences were detected between sham and PSNL groups at either timepoints.
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Mouse mural cells sub-cluster into five groups, resembling cell types previously described in the brain, with one (aaSMC) detected only day 5 after PSNL or sham surgery (A). Acta2 & Tagln high vascular smooth muscle cells (vSMC), arteriolar SMCs enriched for Slit3 and Ctnna3 (aaSMC), Vtn & Abcc9 & Kcnj8 positive pericytes (PC1&2), Adgrf5 negative (PC3) and Acta2 negative pericytes (PC-NC). Expression of marker genes is plotted in B. No prominent differences were detected between sham and PSNL groups at either timepoints.

Journal: bioRxiv

Article Title: A role for fibroblast and mural cell subsets in models of neuropathic pain

doi: 10.1101/2024.12.11.627455

Figure Lengend Snippet: Mouse mural cells sub-cluster into five groups, resembling cell types previously described in the brain, with one (aaSMC) detected only day 5 after PSNL or sham surgery (A). Acta2 & Tagln high vascular smooth muscle cells (vSMC), arteriolar SMCs enriched for Slit3 and Ctnna3 (aaSMC), Vtn & Abcc9 & Kcnj8 positive pericytes (PC1&2), Adgrf5 negative (PC3) and Acta2 negative pericytes (PC-NC). Expression of marker genes is plotted in B. No prominent differences were detected between sham and PSNL groups at either timepoints.

Article Snippet: A vial of human placental pericytes was purchased by PromoCell (#C-12980) and a human peripheral nerve pericyte line was obtained under MTA from Profs.

Techniques: Expressing, Marker

Plotted here is a selection of mediators, the majority of which we identified as differentially expressed in pericytes in our bulk sequencing experiment (Il6, Lif, Clcf1, Ccl2 and Tnc). Their expression patterns appeared to differ across mural cell populations, with significant enrichment of Il6, Ccl2 and Tnc in PC1&2 pericytes, and Clcf1 in Acta2 negative pericytes (PC-NC).

Journal: bioRxiv

Article Title: A role for fibroblast and mural cell subsets in models of neuropathic pain

doi: 10.1101/2024.12.11.627455

Figure Lengend Snippet: Plotted here is a selection of mediators, the majority of which we identified as differentially expressed in pericytes in our bulk sequencing experiment (Il6, Lif, Clcf1, Ccl2 and Tnc). Their expression patterns appeared to differ across mural cell populations, with significant enrichment of Il6, Ccl2 and Tnc in PC1&2 pericytes, and Clcf1 in Acta2 negative pericytes (PC-NC).

Article Snippet: A vial of human placental pericytes was purchased by PromoCell (#C-12980) and a human peripheral nerve pericyte line was obtained under MTA from Profs.

Techniques: Selection, Sequencing, Expressing

Each dot is a well of cells. Filled circles shows the expression levels obtained from media taken from a placental pericyte line, open circles those obtained from a nerve pericyte line. Ctrl = regular pericyte media; other columns: pericytes treated for four hours with TNF (10ng/ml), IL-17 (10ng/ml), TNF+IL-17 (both at 10ng/ml), IL-4 (10ng/ml) or IFNγ (100ng/ml). The red dotted lines represent the upper and lower limits of the standard curves for each protein, i.e. the upper and lower detection limits. Shown here are 1:100 dilutions for IL-6 and NGF and 1:2 dilutions for CCL2.

Journal: bioRxiv

Article Title: A role for fibroblast and mural cell subsets in models of neuropathic pain

doi: 10.1101/2024.12.11.627455

Figure Lengend Snippet: Each dot is a well of cells. Filled circles shows the expression levels obtained from media taken from a placental pericyte line, open circles those obtained from a nerve pericyte line. Ctrl = regular pericyte media; other columns: pericytes treated for four hours with TNF (10ng/ml), IL-17 (10ng/ml), TNF+IL-17 (both at 10ng/ml), IL-4 (10ng/ml) or IFNγ (100ng/ml). The red dotted lines represent the upper and lower limits of the standard curves for each protein, i.e. the upper and lower detection limits. Shown here are 1:100 dilutions for IL-6 and NGF and 1:2 dilutions for CCL2.

Article Snippet: A vial of human placental pericytes was purchased by PromoCell (#C-12980) and a human peripheral nerve pericyte line was obtained under MTA from Profs.

Techniques: Expressing

(A) Representative Western blot of neuronal pSTAT3 and STAT3 following conditioned media incubation. See Supplementary Materials for all full-length Western Blots. (B) Quantification of neuronal pSTAT3/STAT3 following incubation with cytokine-activated pericyte conditioned media (CM). Each dot represents an independent neuron differentiation (T36-43, n=5). N2 = neuronal control medium.

Journal: bioRxiv

Article Title: A role for fibroblast and mural cell subsets in models of neuropathic pain

doi: 10.1101/2024.12.11.627455

Figure Lengend Snippet: (A) Representative Western blot of neuronal pSTAT3 and STAT3 following conditioned media incubation. See Supplementary Materials for all full-length Western Blots. (B) Quantification of neuronal pSTAT3/STAT3 following incubation with cytokine-activated pericyte conditioned media (CM). Each dot represents an independent neuron differentiation (T36-43, n=5). N2 = neuronal control medium.

Article Snippet: A vial of human placental pericytes was purchased by PromoCell (#C-12980) and a human peripheral nerve pericyte line was obtained under MTA from Profs.

Techniques: Western Blot, Incubation, Control