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  • 97
    Alomone Labs trpv1
    Comparative graph of paw withdrawal threshold and latency of the Complete Freund’s Adjuvant (CFA)-induced inflammatory pain subjects after Electroacupuncture (EA) treatment and transient receptor vanilloid member 1 <t>(TRPV1)</t> gene deletion. n = 8 for five groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO underwent ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ tests. The results demonstrated parallel tendencies between the two tests. The CFA injection significantly increases hyperalgesia in mechanical and thermal sensitivity. The significant reducing threshold and latency were increased using 2 Hz EA at ST36 and TRPV1 gene deletion. * p
    Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore clozapine n oxide cno
    Comparative graph of paw withdrawal threshold and latency of the Complete Freund’s Adjuvant (CFA)-induced inflammatory pain subjects after Electroacupuncture (EA) treatment and transient receptor vanilloid member 1 <t>(TRPV1)</t> gene deletion. n = 8 for five groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO underwent ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ tests. The results demonstrated parallel tendencies between the two tests. The CFA injection significantly increases hyperalgesia in mechanical and thermal sensitivity. The significant reducing threshold and latency were increased using 2 Hz EA at ST36 and TRPV1 gene deletion. * p
    Clozapine N Oxide Cno, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC bacillus halodurans c 125
    Comparative graph of paw withdrawal threshold and latency of the Complete Freund’s Adjuvant (CFA)-induced inflammatory pain subjects after Electroacupuncture (EA) treatment and transient receptor vanilloid member 1 <t>(TRPV1)</t> gene deletion. n = 8 for five groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO underwent ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ tests. The results demonstrated parallel tendencies between the two tests. The CFA injection significantly increases hyperalgesia in mechanical and thermal sensitivity. The significant reducing threshold and latency were increased using 2 Hz EA at ST36 and TRPV1 gene deletion. * p
    Bacillus Halodurans C 125, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Comparative graph of paw withdrawal threshold and latency of the Complete Freund’s Adjuvant (CFA)-induced inflammatory pain subjects after Electroacupuncture (EA) treatment and transient receptor vanilloid member 1 (TRPV1) gene deletion. n = 8 for five groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO underwent ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ tests. The results demonstrated parallel tendencies between the two tests. The CFA injection significantly increases hyperalgesia in mechanical and thermal sensitivity. The significant reducing threshold and latency were increased using 2 Hz EA at ST36 and TRPV1 gene deletion. * p

    Journal: International Journal of Molecular Sciences

    Article Title: TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model

    doi: 10.3390/ijms21093312

    Figure Lengend Snippet: Comparative graph of paw withdrawal threshold and latency of the Complete Freund’s Adjuvant (CFA)-induced inflammatory pain subjects after Electroacupuncture (EA) treatment and transient receptor vanilloid member 1 (TRPV1) gene deletion. n = 8 for five groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO underwent ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ tests. The results demonstrated parallel tendencies between the two tests. The CFA injection significantly increases hyperalgesia in mechanical and thermal sensitivity. The significant reducing threshold and latency were increased using 2 Hz EA at ST36 and TRPV1 gene deletion. * p

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone, Jerusalem, Israel), TRPV1, pPKAIIα, pPI3K, and pCREB, prepared in 1% bovine serum albumin solution at 4 °C overnight.

    Techniques: Injection

    The expression levels of pain receptors and involved molecules in the cerebellum lobule V. The immunoblotting images contain five lanes of protein in the following order: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO groups. There are significant decreases of ( A ) TRPV1, ( B ) Voltaged-gated Sodium (Nav)1.7, ( C ) Nav1.8, ( D ) acid sensing ion channel subtype 3 (ASIC3), ( E ) pmTOR, ( F ) pPI3K, ( G ) pAkt, ( H ) pERK, ( I ) receptor for advanced glycation end products (RAGE), ( J ) pPKCε, ( K ) pPKAIIα, ( L ) pNFκB, ( M ) pCREB, and ( N ) S100B protein level in CFA + EA and CFA+KO groups compared to CFA and CFA + SHAM groups (* p

    Journal: International Journal of Molecular Sciences

    Article Title: TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model

    doi: 10.3390/ijms21093312

    Figure Lengend Snippet: The expression levels of pain receptors and involved molecules in the cerebellum lobule V. The immunoblotting images contain five lanes of protein in the following order: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO groups. There are significant decreases of ( A ) TRPV1, ( B ) Voltaged-gated Sodium (Nav)1.7, ( C ) Nav1.8, ( D ) acid sensing ion channel subtype 3 (ASIC3), ( E ) pmTOR, ( F ) pPI3K, ( G ) pAkt, ( H ) pERK, ( I ) receptor for advanced glycation end products (RAGE), ( J ) pPKCε, ( K ) pPKAIIα, ( L ) pNFκB, ( M ) pCREB, and ( N ) S100B protein level in CFA + EA and CFA+KO groups compared to CFA and CFA + SHAM groups (* p

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone, Jerusalem, Israel), TRPV1, pPKAIIα, pPI3K, and pCREB, prepared in 1% bovine serum albumin solution at 4 °C overnight.

    Techniques: Expressing

    Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VIa and VIb. There are 5 subject groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO. ( A ) The effectiveness of EA treatment involves significant reduction of TRPV1 and pNFkB densities in the cerebellum lobule VIa. ( B ) In contrast, there was no nsignificant variance of TRPV1 and pNFkB density in the cerebellum lobule VIb the among five groups of subjects. Scale bar is 50 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model

    doi: 10.3390/ijms21093312

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VIa and VIb. There are 5 subject groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO. ( A ) The effectiveness of EA treatment involves significant reduction of TRPV1 and pNFkB densities in the cerebellum lobule VIa. ( B ) In contrast, there was no nsignificant variance of TRPV1 and pNFkB density in the cerebellum lobule VIb the among five groups of subjects. Scale bar is 50 μm.

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone, Jerusalem, Israel), TRPV1, pPKAIIα, pPI3K, and pCREB, prepared in 1% bovine serum albumin solution at 4 °C overnight.

    Techniques: Immunofluorescence, Staining, Expressing

    The expression levels of pain receptors and involved molecules in the cerebellum lobule VI. The immunoblotting images contain five lanes of protein in the following order: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO groups. There are no significant difference for ( B ) Nav1.7, ( C ) Nav1.8, ( D ) ASIC3, ( E ) pmTOR, ( F ) pPI3K, ( G ) pAkt, ( I ) RAGE, ( J ) pPKCε, ( K ) pPKAIIα, ( L ) pNFκB, ( M ) pCREB, and ( N ) S100B protein level among the five groups of subjects ( p > 0.05). However, the protein density of ( A ) TRPV1 and ( H ) pERK reveal significant decreases in the CFA + KO group compared to the other four wild-type groups (* p

    Journal: International Journal of Molecular Sciences

    Article Title: TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model

    doi: 10.3390/ijms21093312

    Figure Lengend Snippet: The expression levels of pain receptors and involved molecules in the cerebellum lobule VI. The immunoblotting images contain five lanes of protein in the following order: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO groups. There are no significant difference for ( B ) Nav1.7, ( C ) Nav1.8, ( D ) ASIC3, ( E ) pmTOR, ( F ) pPI3K, ( G ) pAkt, ( I ) RAGE, ( J ) pPKCε, ( K ) pPKAIIα, ( L ) pNFκB, ( M ) pCREB, and ( N ) S100B protein level among the five groups of subjects ( p > 0.05). However, the protein density of ( A ) TRPV1 and ( H ) pERK reveal significant decreases in the CFA + KO group compared to the other four wild-type groups (* p

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone, Jerusalem, Israel), TRPV1, pPKAIIα, pPI3K, and pCREB, prepared in 1% bovine serum albumin solution at 4 °C overnight.

    Techniques: Expressing

    Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules V and VII. There are 5 subject groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO. The effectiveness of EA treatment involves significant reduction of TRPV1 and pNFkB densities in the cerebellum lobules ( A ) V and ( B ) VII. Scale bar is 50 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model

    doi: 10.3390/ijms21093312

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules V and VII. There are 5 subject groups: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO. The effectiveness of EA treatment involves significant reduction of TRPV1 and pNFkB densities in the cerebellum lobules ( A ) V and ( B ) VII. Scale bar is 50 μm.

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone, Jerusalem, Israel), TRPV1, pPKAIIα, pPI3K, and pCREB, prepared in 1% bovine serum albumin solution at 4 °C overnight.

    Techniques: Immunofluorescence, Staining, Expressing

    Illustration of inflammatory pain pathways attenuated by EA in the cerebellum. We found that EA at 1 mA, 2 Hz for 20 min and TRPV1 gene deletion can diminish the expression of TRPV1, Nav1.7, Nav1.8, ASIC3, and RAGE nociceptors in the CFA-induced inflammatory pain model in CFA + EA and CFA + KO groups. Furthermore, the reductions cause decreases in the signals of pPI3K, pmTOR, pAkt, pERK, pPKCε, pPKAIIα, pNFkB, pCREB, and S100B under conditions of inflammatory hyperalgesia.

    Journal: International Journal of Molecular Sciences

    Article Title: TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model

    doi: 10.3390/ijms21093312

    Figure Lengend Snippet: Illustration of inflammatory pain pathways attenuated by EA in the cerebellum. We found that EA at 1 mA, 2 Hz for 20 min and TRPV1 gene deletion can diminish the expression of TRPV1, Nav1.7, Nav1.8, ASIC3, and RAGE nociceptors in the CFA-induced inflammatory pain model in CFA + EA and CFA + KO groups. Furthermore, the reductions cause decreases in the signals of pPI3K, pmTOR, pAkt, pERK, pPKCε, pPKAIIα, pNFkB, pCREB, and S100B under conditions of inflammatory hyperalgesia.

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone, Jerusalem, Israel), TRPV1, pPKAIIα, pPI3K, and pCREB, prepared in 1% bovine serum albumin solution at 4 °C overnight.

    Techniques: Expressing

    The expression levels of pain receptors and involved molecules in the cerebellum lobule VII. The immunoblotting images contain five lanes of protein in following order: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO groups. There are significant decreases of ( A ) TRPV1, ( B ) Nav1.7, ( C ) Nav1.8, ( D ) ASIC3, ( E ) pmTOR, ( F ) pPI3K, ( G ) pAkt, ( H ) pERK, ( I ) RAGE, ( J ) pPKCε, ( K ) pPKAIIα, ( L ) pNFκB, ( M ) pCREB and, ( N ) S100B protein level in CFA + EA and CFA + KO groups compared to CFA and CFA + SHAM groups (* p

    Journal: International Journal of Molecular Sciences

    Article Title: TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model

    doi: 10.3390/ijms21093312

    Figure Lengend Snippet: The expression levels of pain receptors and involved molecules in the cerebellum lobule VII. The immunoblotting images contain five lanes of protein in following order: control, CFA, CFA + EA, CFA + SHAM, and CFA + KO groups. There are significant decreases of ( A ) TRPV1, ( B ) Nav1.7, ( C ) Nav1.8, ( D ) ASIC3, ( E ) pmTOR, ( F ) pPI3K, ( G ) pAkt, ( H ) pERK, ( I ) RAGE, ( J ) pPKCε, ( K ) pPKAIIα, ( L ) pNFκB, ( M ) pCREB and, ( N ) S100B protein level in CFA + EA and CFA + KO groups compared to CFA and CFA + SHAM groups (* p

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone, Jerusalem, Israel), TRPV1, pPKAIIα, pPI3K, and pCREB, prepared in 1% bovine serum albumin solution at 4 °C overnight.

    Techniques: Expressing

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Journal: Chinese Medicine

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    doi: 10.1186/s13020-021-00468-5

    Figure Lengend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Article Snippet: Consistent with the data obtained from animal experiments, 50 μg/ml CSBTA significantly inhibited protein contents of PKCε, p-p38 MAPK, and TRPV1, and reduced mRNA levels of PGE2, TNF-α, and CGRP (Figs. and ).

    Techniques: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Journal: Chinese Medicine

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    doi: 10.1186/s13020-021-00468-5

    Figure Lengend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Article Snippet: Consistent with the data obtained from animal experiments, 50 μg/ml CSBTA significantly inhibited protein contents of PKCε, p-p38 MAPK, and TRPV1, and reduced mRNA levels of PGE2, TNF-α, and CGRP (Figs. and ).

    Techniques: Expressing, Western Blot