BrdU Antibody Search Results


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    Abcam rat anti brdu
    Tumors of JPH203-treated mice exhibit an increase in TUNEL-positive nuclei and activation of the amino acid stress response. H E pictures demonstrating PTC/ATC features in ( a ) a vehicle-treated mouse and PTC features in ( b ) a JPH203-treated mouse. c , d <t>Ki67-positive</t> cells and <t>BrdU-positive</t> cells were quantified from each mouse and plotted in the graphs. e TUNEL-positive nuclei were quantified from each mouse and plotted in the graph. f , g Atf5, Slc7a8 and Slc3a2 transcription levels were quantified in vehicle- and JPH203-treated animals. Transcription levels of each gene were normalized to Tuba1a and plotted as fold change relative to vehicle level. All statistical analyses were performed by using a two-tailed Mann-Whitney test
    Rat Anti Brdu, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti brdu/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat anti brdu - by Bioz Stars, 2021-05
    99/100 stars
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    86
    Bio-Rad rat anti brdu
    Altered bone growth dynamics in Tcf12 +/- ; Twist1 +/- mice. ( A ) Lateral views of E13.5 mouse heads show Alkaline phosphatase staining of developing frontal and parietal bones. Dotted lines indicate the bone fronts that will form the coronal suture. Compared to wild types ( n = 5), the fronts were accelerated in all Tcf12 +/- ; Twist1 +/- mutants ( n = 12). Scale bar, 1 mm. ( B ) Dorsal views of skull bones stained with Alizarin Red at birth (P0). Compared to wild types ( n = 5), the fronts were closer together in all Tcf12 +/- ; Twist1 +/- mutants ( n = 11). Scale bar, 1 mm. ( C ) Sections of E14.5 coronal sutures and E16.5 sagittal sutures stained for <t>BrdU</t> (magenta), <t>Sp7</t> protein (green), and DAPI (blue, nuclei). Boxed regions are magnified in lower panels, with yellow dotted lines indicating the regions of interest (ROI) used for quantification. fb, frontal bone; pb, parietal bone. Scale bar, 100 µm. ( D–F ) Quantification of Sp7 + osteoblasts per ROI ( D ), BrdU + Sp7 - bone front cells per ROI ( E ), and the percentage of Sp7 + osteoblasts that are BrdU + in the ROI ( F ). Cell counts were performed at the developing coronal sutures (cs, four wild types, three mutants) and sagittal sutures (ss, six wild types, six mutants). p values were determined by Student’s t-tests; error bars represent standard error of the mean.
    Rat Anti Brdu, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti brdu/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat anti brdu - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    N/A
    BrdU Antibody is a Mouse Monoclonal antibody against BrdU The halogenated pyrimidine thymidine analog bromodeoxyuridine BrdU is incorporated into newly synthesized DNA strands of S phase cells and is useful
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    N/A
    Mouse monoclonal BRDU antibody
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    N/A
    It reacts with Bromodeoxyuridine BrdU in single stranded DNA produced by partial denaturation of double stranded DNA BrdU coupled to a protein carrier as well as free BrdU BrdU is
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    N/A
    Rabbit polyclonal antibody to BrdU Isotype Note IgG Host Note Rabbit Conjugation Note Unconjugated Application Note ELISA IHC P IHC Fr IF ICC
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    Image Search Results


    Tumors of JPH203-treated mice exhibit an increase in TUNEL-positive nuclei and activation of the amino acid stress response. H E pictures demonstrating PTC/ATC features in ( a ) a vehicle-treated mouse and PTC features in ( b ) a JPH203-treated mouse. c , d Ki67-positive cells and BrdU-positive cells were quantified from each mouse and plotted in the graphs. e TUNEL-positive nuclei were quantified from each mouse and plotted in the graph. f , g Atf5, Slc7a8 and Slc3a2 transcription levels were quantified in vehicle- and JPH203-treated animals. Transcription levels of each gene were normalized to Tuba1a and plotted as fold change relative to vehicle level. All statistical analyses were performed by using a two-tailed Mann-Whitney test

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: The LAT1 inhibitor JPH203 reduces growth of thyroid carcinoma in a fully immunocompetent mouse model

    doi: 10.1186/s13046-018-0907-z

    Figure Lengend Snippet: Tumors of JPH203-treated mice exhibit an increase in TUNEL-positive nuclei and activation of the amino acid stress response. H E pictures demonstrating PTC/ATC features in ( a ) a vehicle-treated mouse and PTC features in ( b ) a JPH203-treated mouse. c , d Ki67-positive cells and BrdU-positive cells were quantified from each mouse and plotted in the graphs. e TUNEL-positive nuclei were quantified from each mouse and plotted in the graph. f , g Atf5, Slc7a8 and Slc3a2 transcription levels were quantified in vehicle- and JPH203-treated animals. Transcription levels of each gene were normalized to Tuba1a and plotted as fold change relative to vehicle level. All statistical analyses were performed by using a two-tailed Mann-Whitney test

    Article Snippet: Primary antibodies anti-Ki67 (Abcam cat. no. ab16667,) and anti-BrdU (Abcam cat. no. ab6326,) were incubated overnight at 4 °C.

    Techniques: Mouse Assay, TUNEL Assay, Activation Assay, Two Tailed Test, MANN-WHITNEY

    Altered bone growth dynamics in Tcf12 +/- ; Twist1 +/- mice. ( A ) Lateral views of E13.5 mouse heads show Alkaline phosphatase staining of developing frontal and parietal bones. Dotted lines indicate the bone fronts that will form the coronal suture. Compared to wild types ( n = 5), the fronts were accelerated in all Tcf12 +/- ; Twist1 +/- mutants ( n = 12). Scale bar, 1 mm. ( B ) Dorsal views of skull bones stained with Alizarin Red at birth (P0). Compared to wild types ( n = 5), the fronts were closer together in all Tcf12 +/- ; Twist1 +/- mutants ( n = 11). Scale bar, 1 mm. ( C ) Sections of E14.5 coronal sutures and E16.5 sagittal sutures stained for BrdU (magenta), Sp7 protein (green), and DAPI (blue, nuclei). Boxed regions are magnified in lower panels, with yellow dotted lines indicating the regions of interest (ROI) used for quantification. fb, frontal bone; pb, parietal bone. Scale bar, 100 µm. ( D–F ) Quantification of Sp7 + osteoblasts per ROI ( D ), BrdU + Sp7 - bone front cells per ROI ( E ), and the percentage of Sp7 + osteoblasts that are BrdU + in the ROI ( F ). Cell counts were performed at the developing coronal sutures (cs, four wild types, three mutants) and sagittal sutures (ss, six wild types, six mutants). p values were determined by Student’s t-tests; error bars represent standard error of the mean.

    Journal: eLife

    Article Title: Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome

    doi: 10.7554/eLife.37024

    Figure Lengend Snippet: Altered bone growth dynamics in Tcf12 +/- ; Twist1 +/- mice. ( A ) Lateral views of E13.5 mouse heads show Alkaline phosphatase staining of developing frontal and parietal bones. Dotted lines indicate the bone fronts that will form the coronal suture. Compared to wild types ( n = 5), the fronts were accelerated in all Tcf12 +/- ; Twist1 +/- mutants ( n = 12). Scale bar, 1 mm. ( B ) Dorsal views of skull bones stained with Alizarin Red at birth (P0). Compared to wild types ( n = 5), the fronts were closer together in all Tcf12 +/- ; Twist1 +/- mutants ( n = 11). Scale bar, 1 mm. ( C ) Sections of E14.5 coronal sutures and E16.5 sagittal sutures stained for BrdU (magenta), Sp7 protein (green), and DAPI (blue, nuclei). Boxed regions are magnified in lower panels, with yellow dotted lines indicating the regions of interest (ROI) used for quantification. fb, frontal bone; pb, parietal bone. Scale bar, 100 µm. ( D–F ) Quantification of Sp7 + osteoblasts per ROI ( D ), BrdU + Sp7 - bone front cells per ROI ( E ), and the percentage of Sp7 + osteoblasts that are BrdU + in the ROI ( F ). Cell counts were performed at the developing coronal sutures (cs, four wild types, three mutants) and sagittal sutures (ss, six wild types, six mutants). p values were determined by Student’s t-tests; error bars represent standard error of the mean.

    Article Snippet: Immunohistochemistry was performed using rat anti-BrdU (MCA2060 GA, Bio-Rad), rabbit anti-Osx/Sp7 (sc-22536-r, Santa Cruz), goat anti-Grem1 (PA5-47973, Thermo Fisher), or goat anti-Gli1 (AF3455, R and D Systems) diluted in 1% BSA/PBS and incubated overnight at 4°C.

    Techniques: Mouse Assay, Staining

    Altered proliferation and osteoblast production at mutant zebrafish bone fronts. ( A ) Dissected skullcaps were stained for BrdU (magenta) and Sp7 protein (green) at 9 mm. Top panels show maximum intensity projections of whole skull volumes, and middle panels are the same volumes but processed to extract the bone fronts (note that much of the BrdU staining in the center of the top images is in the skin). Bottom panels show enlarged regions of the osteogenic fronts (dotted rectangles). White arrows show proliferative osteoblasts (BrdU + /Sp7 + ) and magenta arrows show adjacent proliferative Sp7 - cells. fb, frontal bone; pb, parietal bone. Scale bars, 300 µm for whole skull view, 100 µm for enlarged view. ( B, C ) Based on the extracted osteogenic fronts (middle panels in A), we quantified the percentage of Sp7 + osteoblasts that were BrdU + ( B ) and the number of adjacent BrdU + /Sp7 - cells per area ( C ). Wild-type controls, n = 20; tcf12; twist1b mutants, n = 28. p values were determined by a Student’s t-test; error bars represent standard error of the mean.

    Journal: eLife

    Article Title: Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome

    doi: 10.7554/eLife.37024

    Figure Lengend Snippet: Altered proliferation and osteoblast production at mutant zebrafish bone fronts. ( A ) Dissected skullcaps were stained for BrdU (magenta) and Sp7 protein (green) at 9 mm. Top panels show maximum intensity projections of whole skull volumes, and middle panels are the same volumes but processed to extract the bone fronts (note that much of the BrdU staining in the center of the top images is in the skin). Bottom panels show enlarged regions of the osteogenic fronts (dotted rectangles). White arrows show proliferative osteoblasts (BrdU + /Sp7 + ) and magenta arrows show adjacent proliferative Sp7 - cells. fb, frontal bone; pb, parietal bone. Scale bars, 300 µm for whole skull view, 100 µm for enlarged view. ( B, C ) Based on the extracted osteogenic fronts (middle panels in A), we quantified the percentage of Sp7 + osteoblasts that were BrdU + ( B ) and the number of adjacent BrdU + /Sp7 - cells per area ( C ). Wild-type controls, n = 20; tcf12; twist1b mutants, n = 28. p values were determined by a Student’s t-test; error bars represent standard error of the mean.

    Article Snippet: Immunohistochemistry was performed using rat anti-BrdU (MCA2060 GA, Bio-Rad), rabbit anti-Osx/Sp7 (sc-22536-r, Santa Cruz), goat anti-Grem1 (PA5-47973, Thermo Fisher), or goat anti-Gli1 (AF3455, R and D Systems) diluted in 1% BSA/PBS and incubated overnight at 4°C.

    Techniques: Mutagenesis, Staining, BrdU Staining