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    • tbs t buffer  (Alomone Labs)
    88
    Alomone Labs tbs t buffer
    Enhanced P2X3 nociceptor expression in the DRG and blocking effect of P2X3 receptor antagonist on the empathic mechanical pain hypersensitivity in the CO rats. (A–C) showing representative immunoblotting bands and quantitative analysis of P2X3 and <t>TRPV1</t> expressions in the L4-L5 DRG neurons in the CC, CO and NCO rats (sample n = 6 for each group, however, each sample contains DRGs obtained from at least 9 rats) following social interaction. The relative value density for bands of P2X3 and TRPV1 receptors in the CO and NCO rats was normalized to the value of P2X3 and TRPV1 receptors in the CC rats, respectively. (D) showing peripheral effects of P2X3 receptor antagonist (A317491) on development of the empathic mechanical pain hypersensitivity in the CO rats (saline, n = 9) and A317491, n = 16). (E) showing peripheral effects of P2X3 receptor antagonist (A317491) on baseline PWMT in the CC rats (A317491 and saline, n = 8 for each group). CC, cagemate control; CO, cagemate observer; DRG, dorsal root ganglia; NCO, non-cagemate observer; PWMT, paw withdraw mechanical threshold. ††† P
    Tbs T Buffer, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Enhanced P2X3 nociceptor expression in the DRG and blocking effect of P2X3 receptor antagonist on the empathic mechanical pain hypersensitivity in the CO rats. (A–C) showing representative immunoblotting bands and quantitative analysis of P2X3 and TRPV1 expressions in the L4-L5 DRG neurons in the CC, CO and NCO rats (sample n = 6 for each group, however, each sample contains DRGs obtained from at least 9 rats) following social interaction. The relative value density for bands of P2X3 and TRPV1 receptors in the CO and NCO rats was normalized to the value of P2X3 and TRPV1 receptors in the CC rats, respectively. (D) showing peripheral effects of P2X3 receptor antagonist (A317491) on development of the empathic mechanical pain hypersensitivity in the CO rats (saline, n = 9) and A317491, n = 16). (E) showing peripheral effects of P2X3 receptor antagonist (A317491) on baseline PWMT in the CC rats (A317491 and saline, n = 8 for each group). CC, cagemate control; CO, cagemate observer; DRG, dorsal root ganglia; NCO, non-cagemate observer; PWMT, paw withdraw mechanical threshold. ††† P

    Journal: Frontiers in Neural Circuits

    Article Title: The Locus Coeruleus–Norepinephrine System Mediates Empathy for Pain through Selective Up-Regulation of P2X3 Receptor in Dorsal Root Ganglia in Rats

    doi: 10.3389/fncir.2017.00066

    Figure Lengend Snippet: Enhanced P2X3 nociceptor expression in the DRG and blocking effect of P2X3 receptor antagonist on the empathic mechanical pain hypersensitivity in the CO rats. (A–C) showing representative immunoblotting bands and quantitative analysis of P2X3 and TRPV1 expressions in the L4-L5 DRG neurons in the CC, CO and NCO rats (sample n = 6 for each group, however, each sample contains DRGs obtained from at least 9 rats) following social interaction. The relative value density for bands of P2X3 and TRPV1 receptors in the CO and NCO rats was normalized to the value of P2X3 and TRPV1 receptors in the CC rats, respectively. (D) showing peripheral effects of P2X3 receptor antagonist (A317491) on development of the empathic mechanical pain hypersensitivity in the CO rats (saline, n = 9) and A317491, n = 16). (E) showing peripheral effects of P2X3 receptor antagonist (A317491) on baseline PWMT in the CC rats (A317491 and saline, n = 8 for each group). CC, cagemate control; CO, cagemate observer; DRG, dorsal root ganglia; NCO, non-cagemate observer; PWMT, paw withdraw mechanical threshold. ††† P

    Article Snippet: Primary antibodies used in the present study were rabbit anti-P2X3 (1:400, Millipore, USA), mouse anti-TRPV1 (1:200, Alomone, Israel), mouse anti-actin (1:2000, Sigma, USA).

    Techniques: Expressing, Blocking Assay